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The map label for this gene is dnaG [H]

Identifier: 239616749

GI number: 239616749

Start: 360672

End: 362405

Strand: Direct

Name: dnaG [H]

Synonym: Kole_0342

Alternate gene names: 239616749

Gene position: 360672-362405 (Clockwise)

Preceding gene: 239616748

Following gene: 239616750

Centisome position: 15.67

GC content: 38.41

Gene sequence:

>1734_bases
GTGTTCGATAAGGAGATAATTGACAGAATCAAAAGCTCCATTGATATTGTGGAGCTTGTTGGTCGTTATGTTAACCTCCA
GAAGTCTGGAAGTTATTACAGAGGACTATGCCCTTTTCATAGCGACAGCGATCCATCTTTTTATGTTAGTCCCGGCAAGC
AATTTTTCCATTGCTTTGGATGTGGTGTTTCTGGAGACGTGATAACTTTCTATGAAAAAATAGAAAATCTGCCCTTTTCC
GAGGCAGTGGAAAAGCTAGCTGAAATCGCAGGCATTGAGTTACCAAAAAAATATTCCGTCAGAACGGAATACGACAAATA
TACACAATCCCTAAATATTCTGGCTGAAGCCTATCATAAGGAACTTTTATCGGGAAAACATCCGGATGTCCTTGAGTACC
TTTTAAAAGAAAGGGGTTTCTCAGAAGAAACATTACGTGAATTTCGTATAGGTTTTTCACCAGGAAATCGAACTTTTTTG
AAGAAACTGGTGTCAAATTTCGGGGTAAAATTGGAAGAACTTTTAAAAGCAGGCGTTCTGGTTAAGAAAAATAGTGATTA
CTTCGACAGATTCAGCAAAAGAATTATAATACCAATACACAATGAGAATGGAAAAGTTATTGGTTTTGGCGGAAGAATAA
TACCTGGAGAAACTGGAAGTAAGTACATAAATTCACCTGAAACGCGGTATTTTGTTAAAGGTCGAGTGTTGTTCAATTTA
CACCGAGCAAAAAAGAGTGTAAAAGAACTTGATTATGTTGTCATAACAGAAGGTTACTTTGATTCGATGGCGCTTTATGA
AGCCGGAATTGAAAACAACGTAGGGGTTCTTGGAACAAATTTAACCCGCCAGCACTTAAAATTACTAAAAACCTATACAA
ACAATCTTCTTTTTTTAATGGACTCTGATAGTGCTGGAAAAAATGCAACTCTCCGTTCACTCGATCTAGCTGAAAGCGAG
GGGTTTTCAACCGCGGTAGCAAAAATAACCGGCACAAAAGATGCCGGAGAACTCTTTAAAAACGAGGGACCAAAAGCCCT
TGTGGATACACTCAAGAACGCGATACCCGGCGCAGTATTTCGAGTGGATTATCTCGCGCTAAAGGTAGATATTACTACCC
CCCAGGGAAAACAAAAACTTCTGGAATTCCTGATTCCCACATTATCCAGGTACCTCCAAAGAGCGGATAATGCGGGTTTC
CAACTCACAGTCAAAGCCATTTCTGAAAAAACAAATCTCCTCATCAAGGAAATAATAGCCGTGGCAAAAGGAAGGGAGAA
ACCACAAAAGATTTTAAGGGAACGTAGTACTTTTAAACTCAAACAAATAGAAAAAGACTTGCTAAGCATTTATTTTGGGC
ATCCAGAATTACGAAGCGAGGTCGCAAGAGTAGTTGATTTTTTAGAAACGTCAGAAGCTTTCAGGTATCTGGTGAAATTT
ATAAAGAAGAAACAGGACTTCAACGAGATTATTGATGAAATAGACGAAAAAATAGGACCTTCTCTTATGGAAGCGGTTGA
ATATTCTACCGATATTTCGATGGCACGTAAAATGTTAAAAGATATCGAGACGCATGTTGATAAAAAAATAATTCGCGAAC
AAATTGAGGAAATAGATAGAAAACTTAGAAAAGTAATCGACCCAGCTATGAAAGAATCACTGATGATTCGTCGCATGGAA
CTCACAAGGTTACTAAAAAGAACCTTGAAGGGTGGTGAAGCTGATGGCAGATAA

Upstream 100 bases:

>100_bases
TCAAAAAAGCTAGAAGAGCTCCTCAGTTCTCCAAACGTTAAGCTTCTTTTTGGTTTCAATGATGGGCCTCGTCGGCCCAT
CTTCTCTTGGTGGTGGTTGA

Downstream 100 bases:

>100_bases
AAAGAATAAGGCAAAAATGGTGGAGGAACCAAAAGAAAAAAACTCTTCACAAAAGAAAACAACTGAAGTAGTCATGCTTT
CAGACAAAGAGGTTCAATCA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MFDKEIIDRIKSSIDIVELVGRYVNLQKSGSYYRGLCPFHSDSDPSFYVSPGKQFFHCFGCGVSGDVITFYEKIENLPFS
EAVEKLAEIAGIELPKKYSVRTEYDKYTQSLNILAEAYHKELLSGKHPDVLEYLLKERGFSEETLREFRIGFSPGNRTFL
KKLVSNFGVKLEELLKAGVLVKKNSDYFDRFSKRIIIPIHNENGKVIGFGGRIIPGETGSKYINSPETRYFVKGRVLFNL
HRAKKSVKELDYVVITEGYFDSMALYEAGIENNVGVLGTNLTRQHLKLLKTYTNNLLFLMDSDSAGKNATLRSLDLAESE
GFSTAVAKITGTKDAGELFKNEGPKALVDTLKNAIPGAVFRVDYLALKVDITTPQGKQKLLEFLIPTLSRYLQRADNAGF
QLTVKAISEKTNLLIKEIIAVAKGREKPQKILRERSTFKLKQIEKDLLSIYFGHPELRSEVARVVDFLETSEAFRYLVKF
IKKKQDFNEIIDEIDEKIGPSLMEAVEYSTDISMARKMLKDIETHVDKKIIREQIEEIDRKLRKVIDPAMKESLMIRRME
LTRLLKRTLKGGEADGR

Sequences:

>Translated_577_residues
MFDKEIIDRIKSSIDIVELVGRYVNLQKSGSYYRGLCPFHSDSDPSFYVSPGKQFFHCFGCGVSGDVITFYEKIENLPFS
EAVEKLAEIAGIELPKKYSVRTEYDKYTQSLNILAEAYHKELLSGKHPDVLEYLLKERGFSEETLREFRIGFSPGNRTFL
KKLVSNFGVKLEELLKAGVLVKKNSDYFDRFSKRIIIPIHNENGKVIGFGGRIIPGETGSKYINSPETRYFVKGRVLFNL
HRAKKSVKELDYVVITEGYFDSMALYEAGIENNVGVLGTNLTRQHLKLLKTYTNNLLFLMDSDSAGKNATLRSLDLAESE
GFSTAVAKITGTKDAGELFKNEGPKALVDTLKNAIPGAVFRVDYLALKVDITTPQGKQKLLEFLIPTLSRYLQRADNAGF
QLTVKAISEKTNLLIKEIIAVAKGREKPQKILRERSTFKLKQIEKDLLSIYFGHPELRSEVARVVDFLETSEAFRYLVKF
IKKKQDFNEIIDEIDEKIGPSLMEAVEYSTDISMARKMLKDIETHVDKKIIREQIEEIDRKLRKVIDPAMKESLMIRRME
LTRLLKRTLKGGEADGR
>Mature_577_residues
MFDKEIIDRIKSSIDIVELVGRYVNLQKSGSYYRGLCPFHSDSDPSFYVSPGKQFFHCFGCGVSGDVITFYEKIENLPFS
EAVEKLAEIAGIELPKKYSVRTEYDKYTQSLNILAEAYHKELLSGKHPDVLEYLLKERGFSEETLREFRIGFSPGNRTFL
KKLVSNFGVKLEELLKAGVLVKKNSDYFDRFSKRIIIPIHNENGKVIGFGGRIIPGETGSKYINSPETRYFVKGRVLFNL
HRAKKSVKELDYVVITEGYFDSMALYEAGIENNVGVLGTNLTRQHLKLLKTYTNNLLFLMDSDSAGKNATLRSLDLAESE
GFSTAVAKITGTKDAGELFKNEGPKALVDTLKNAIPGAVFRVDYLALKVDITTPQGKQKLLEFLIPTLSRYLQRADNAGF
QLTVKAISEKTNLLIKEIIAVAKGREKPQKILRERSTFKLKQIEKDLLSIYFGHPELRSEVARVVDFLETSEAFRYLVKF
IKKKQDFNEIIDEIDEKIGPSLMEAVEYSTDISMARKMLKDIETHVDKKIIREQIEEIDRKLRKVIDPAMKESLMIRRME
LTRLLKRTLKGGEADGR

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=474, Percent_Identity=33.1223628691983, Blast_Score=239, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65704; Mature: 65704

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKEIIDRIKSSIDIVELVGRYVNLQKSGSYYRGLCPFHSDSDPSFYVSPGKQFFHCFG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHC
CGVSGDVITFYEKIENLPFSEAVEKLAEIAGIELPKKYSVRTEYDKYTQSLNILAEAYHK
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELLSGKHPDVLEYLLKERGFSEETLREFRIGFSPGNRTFLKKLVSNFGVKLEELLKAGVL
HHHCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCE
VKKNSDYFDRFSKRIIIPIHNENGKVIGFGGRIIPGETGSKYINSPETRYFVKGRVLFNL
EECCCHHHHHHCCEEEEEEECCCCCEEEECCEECCCCCCCCCCCCCCCEEEEEHHHHHHH
HRAKKSVKELDYVVITEGYFDSMALYEAGIENNVGVLGTNLTRQHLKLLKTYTNNLLFLM
HHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCEEEEE
DSDSAGKNATLRSLDLAESEGFSTAVAKITGTKDAGELFKNEGPKALVDTLKNAIPGAVF
ECCCCCCCCEEHCCCCHHCCCCHHHHHEECCCCHHHHHHHCCCCHHHHHHHHHHCCCHHE
RVDYLALKVDITTPQGKQKLLEFLIPTLSRYLQRADNAGFQLTVKAISEKTNLLIKEIIA
EEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
VAKGREKPQKILRERSTFKLKQIEKDLLSIYFGHPELRSEVARVVDFLETSEAFRYLVKF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IKKKQDFNEIIDEIDEKIGPSLMEAVEYSTDISMARKMLKDIETHVDKKIIREQIEEIDR
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRKVIDPAMKESLMIRRMELTRLLKRTLKGGEADGR
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFDKEIIDRIKSSIDIVELVGRYVNLQKSGSYYRGLCPFHSDSDPSFYVSPGKQFFHCFG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHC
CGVSGDVITFYEKIENLPFSEAVEKLAEIAGIELPKKYSVRTEYDKYTQSLNILAEAYHK
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELLSGKHPDVLEYLLKERGFSEETLREFRIGFSPGNRTFLKKLVSNFGVKLEELLKAGVL
HHHCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCE
VKKNSDYFDRFSKRIIIPIHNENGKVIGFGGRIIPGETGSKYINSPETRYFVKGRVLFNL
EECCCHHHHHHCCEEEEEEECCCCCEEEECCEECCCCCCCCCCCCCCCEEEEEHHHHHHH
HRAKKSVKELDYVVITEGYFDSMALYEAGIENNVGVLGTNLTRQHLKLLKTYTNNLLFLM
HHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCEEEEE
DSDSAGKNATLRSLDLAESEGFSTAVAKITGTKDAGELFKNEGPKALVDTLKNAIPGAVF
ECCCCCCCCEEHCCCCHHCCCCHHHHHEECCCCHHHHHHHCCCCHHHHHHHHHHCCCHHE
RVDYLALKVDITTPQGKQKLLEFLIPTLSRYLQRADNAGFQLTVKAISEKTNLLIKEIIA
EEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
VAKGREKPQKILRERSTFKLKQIEKDLLSIYFGHPELRSEVARVVDFLETSEAFRYLVKF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IKKKQDFNEIIDEIDEKIGPSLMEAVEYSTDISMARKMLKDIETHVDKKIIREQIEEIDR
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRKVIDPAMKESLMIRRMELTRLLKRTLKGGEADGR
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]