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The map label for this gene is metK

Identifier: 239616692

GI number: 239616692

Start: 310941

End: 312125

Strand: Direct

Name: metK

Synonym: Kole_0285

Alternate gene names: 239616692

Gene position: 310941-312125 (Clockwise)

Preceding gene: 239616691

Following gene: 239616693

Centisome position: 13.51

GC content: 43.97

Gene sequence:

>1185_bases
ATGAAAAAATGGCTGTTCACCAGTGAAAGTGTTACAGAGGGTCACCCCGACAAAATTGCAGATCAGGTATCAGATGCTAT
TTTGGATGCGATGCTTGAGCAGGATCCTGAATCACGAGTAGCTGTTGAAACGTTGGTGGCAACAGGTGTTGCTGTCGTCG
CAGGAGAGGTAACTACAAAGGCTTACGTTGACATTCCGAGAATCGTAAGAGATACCATATTAGAGATAGGTTATACAAGA
GCCAAGTTTGGTTTTGATGGGGAAACCTGTGCCGTTCTGACAAGTATCGATGAACAGTCGCCGGATATTGCCCTTGGTGT
TAATAAATCTTTAGAAGCAAAGAATGACGGCAAGGATAAATATGACATTATAGGTGCCGGAGATCAGGGTATGATGTTTG
GTTATGCAACGAATGAAACAGAAGAGATGATGCCTCTTCCGATTGTCCTTGCTCACAATCTTGCACGGAGACTCGCAACG
GTCAGAAAAGAAAGGATCGTTGAAGGCTTCCGACCGGATGGTAAAACACAGGTAACTGTTCTCTATGAAGACGGGAAACC
AATCGGAGTCAAAACAATAGTTGTTTCCACACAGCACGATCCAGCCATGACTCAAGAAGAGATAGAACAGCTTGTGAAAG
AGCATGTTATCAAGGCTGTAGTACCTGAAGAAATGATGTTAGATGGAATAGAGACCTTTGTTAATCCCACAGGAAGGTTT
GTGAAAGGCGGCCCTGCGGCAGATACAGGACTTACAGGAAGAAAAATAATCGTAGATACTTACGGTGGATGGATACCTCA
TGGAGGTGGAGCCTTCAGCGGTAAGGATCCAACAAAGGTTGATAGATCAGCTCATTATATGGCGAGATACGTTGCCAAAA
ACATTGTAGCTGCCGGACTTGCTGATAGAGTCACTCTTCAGATTGCTTATGCTATTGGTGTCGCTCATCCTGTTTCATTC
ATGATTGATGCTCATGGAACGGAAAAGGTTGCCCTTGAAAAGCTTGAAAAAGTTGTCAAAGAGGTCTTTGATTTCAGGCC
TGCCGCTATCATCGACAAGCTCAATCTTAGAAGACCTATCTATAAACAGGTTGCCGCTTACGGGCATTTTGGGAGAATAG
ACGTAGAGGTTCCATGGGAAAAATTGGATGCCGTAGATGAACTGAAAAGGGCTTTCAATATGTAA

Upstream 100 bases:

>100_bases
CCATTTCTTTTGATATAATAGGTGGGCGGTTCATTTCTAAAGAAGAATACGAACGTGAAAGTCAACAAACATAGAATTAC
AGATAAAAGGAGGAACGCTG

Downstream 100 bases:

>100_bases
AATCTTGTGATAAAATGATGCAGTAGAGTTTGAAAGGAGGGCAAATGAGTGCCAAATATTAAGTCGGCTGCCAAGCGTGT
GAGGCAAAATAAGGTTAGAA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKKWLFTSESVTEGHPDKIADQVSDAILDAMLEQDPESRVAVETLVATGVAVVAGEVTTKAYVDIPRIVRDTILEIGYTR
AKFGFDGETCAVLTSIDEQSPDIALGVNKSLEAKNDGKDKYDIIGAGDQGMMFGYATNETEEMMPLPIVLAHNLARRLAT
VRKERIVEGFRPDGKTQVTVLYEDGKPIGVKTIVVSTQHDPAMTQEEIEQLVKEHVIKAVVPEEMMLDGIETFVNPTGRF
VKGGPAADTGLTGRKIIVDTYGGWIPHGGGAFSGKDPTKVDRSAHYMARYVAKNIVAAGLADRVTLQIAYAIGVAHPVSF
MIDAHGTEKVALEKLEKVVKEVFDFRPAAIIDKLNLRRPIYKQVAAYGHFGRIDVEVPWEKLDAVDELKRAFNM

Sequences:

>Translated_394_residues
MKKWLFTSESVTEGHPDKIADQVSDAILDAMLEQDPESRVAVETLVATGVAVVAGEVTTKAYVDIPRIVRDTILEIGYTR
AKFGFDGETCAVLTSIDEQSPDIALGVNKSLEAKNDGKDKYDIIGAGDQGMMFGYATNETEEMMPLPIVLAHNLARRLAT
VRKERIVEGFRPDGKTQVTVLYEDGKPIGVKTIVVSTQHDPAMTQEEIEQLVKEHVIKAVVPEEMMLDGIETFVNPTGRF
VKGGPAADTGLTGRKIIVDTYGGWIPHGGGAFSGKDPTKVDRSAHYMARYVAKNIVAAGLADRVTLQIAYAIGVAHPVSF
MIDAHGTEKVALEKLEKVVKEVFDFRPAAIIDKLNLRRPIYKQVAAYGHFGRIDVEVPWEKLDAVDELKRAFNM
>Mature_394_residues
MKKWLFTSESVTEGHPDKIADQVSDAILDAMLEQDPESRVAVETLVATGVAVVAGEVTTKAYVDIPRIVRDTILEIGYTR
AKFGFDGETCAVLTSIDEQSPDIALGVNKSLEAKNDGKDKYDIIGAGDQGMMFGYATNETEEMMPLPIVLAHNLARRLAT
VRKERIVEGFRPDGKTQVTVLYEDGKPIGVKTIVVSTQHDPAMTQEEIEQLVKEHVIKAVVPEEMMLDGIETFVNPTGRF
VKGGPAADTGLTGRKIIVDTYGGWIPHGGGAFSGKDPTKVDRSAHYMARYVAKNIVAAGLADRVTLQIAYAIGVAHPVSF
MIDAHGTEKVALEKLEKVVKEVFDFRPAAIIDKLNLRRPIYKQVAAYGHFGRIDVEVPWEKLDAVDELKRAFNM

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=381, Percent_Identity=54.0682414698163, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI4557737, Length=381, Percent_Identity=53.5433070866142, Blast_Score=383, Evalue=1e-106,
Organism=Escherichia coli, GI1789311, Length=396, Percent_Identity=57.3232323232323, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=51.1627906976744, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17538498, Length=383, Percent_Identity=52.4804177545692, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17538494, Length=383, Percent_Identity=52.2193211488251, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25145633, Length=384, Percent_Identity=52.6041666666667, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565905, Length=383, Percent_Identity=47.5195822454308, Blast_Score=319, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI32565911, Length=334, Percent_Identity=50.5988023952096, Blast_Score=308, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI25145635, Length=334, Percent_Identity=50.8982035928144, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI32565903, Length=309, Percent_Identity=50.4854368932039, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI32565909, Length=76, Percent_Identity=59.2105263157895, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323209, Length=382, Percent_Identity=52.3560209424084, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6320710, Length=386, Percent_Identity=51.8134715025907, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45552159, Length=384, Percent_Identity=51.5625, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580529, Length=384, Percent_Identity=51.5625, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580537, Length=384, Percent_Identity=51.5625, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580533, Length=384, Percent_Identity=51.5625, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580535, Length=384, Percent_Identity=51.5625, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580531, Length=384, Percent_Identity=51.5625, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580527, Length=382, Percent_Identity=50.7853403141361, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24580525, Length=382, Percent_Identity=50.7853403141361, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24580541, Length=369, Percent_Identity=49.5934959349593, Blast_Score=327, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24580539, Length=126, Percent_Identity=50.7936507936508, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_KOSOT (C5CD66)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002940014.1
- GeneID:   7969037
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_0285
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43196; Mature: 43196

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLFTSESVTEGHPDKIADQVSDAILDAMLEQDPESRVAVETLVATGVAVVAGEVTTK
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHEECCHHHH
AYVDIPRIVRDTILEIGYTRAKFGFDGETCAVLTSIDEQSPDIALGVNKSLEAKNDGKDK
HHHHHHHHHHHHHHHHCCHHEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCC
YDIIGAGDQGMMFGYATNETEEMMPLPIVLAHNLARRLATVRKERIVEGFRPDGKTQVTV
EEEEECCCCCEEEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LYEDGKPIGVKTIVVSTQHDPAMTQEEIEQLVKEHVIKAVVPEEMMLDGIETFVNPTGRF
EEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCE
VKGGPAADTGLTGRKIIVDTYGGWIPHGGGAFSGKDPTKVDRSAHYMARYVAKNIVAAGL
EECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ADRVTLQIAYAIGVAHPVSFMIDAHGTEKVALEKLEKVVKEVFDFRPAAIIDKLNLRRPI
HHHHHEEEHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHH
YKQVAAYGHFGRIDVEVPWEKLDAVDELKRAFNM
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKWLFTSESVTEGHPDKIADQVSDAILDAMLEQDPESRVAVETLVATGVAVVAGEVTTK
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHEECCHHHH
AYVDIPRIVRDTILEIGYTRAKFGFDGETCAVLTSIDEQSPDIALGVNKSLEAKNDGKDK
HHHHHHHHHHHHHHHHCCHHEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCC
YDIIGAGDQGMMFGYATNETEEMMPLPIVLAHNLARRLATVRKERIVEGFRPDGKTQVTV
EEEEECCCCCEEEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LYEDGKPIGVKTIVVSTQHDPAMTQEEIEQLVKEHVIKAVVPEEMMLDGIETFVNPTGRF
EEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCE
VKGGPAADTGLTGRKIIVDTYGGWIPHGGGAFSGKDPTKVDRSAHYMARYVAKNIVAAGL
EECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ADRVTLQIAYAIGVAHPVSFMIDAHGTEKVALEKLEKVVKEVFDFRPAAIIDKLNLRRPI
HHHHHEEEHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHH
YKQVAAYGHFGRIDVEVPWEKLDAVDELKRAFNM
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA