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The map label for this gene is speE [H]

Identifier: 239616678

GI number: 239616678

Start: 296023

End: 296889

Strand: Direct

Name: speE [H]

Synonym: Kole_0271

Alternate gene names: 239616678

Gene position: 296023-296889 (Clockwise)

Preceding gene: 239616677

Following gene: 239616679

Centisome position: 12.86

GC content: 39.56

Gene sequence:

>867_bases
GTGAAAAAAGATCTTAGAACGATGGATCATCTTATGTACATGGAATATTATACTGGTAGAAACGCAGGCATTTTTATGAA
GATAAAAAAGATGTTACACTCTTACCAAAGTCCTTATCAAAGGATAGATATATTCGAACATCCCGACTTTGGAAAGGTAT
TTGCTCTTGACGGTATAACGATGATGACGGAAGTGGATGAATTCATGTACCACGAAATGCTGGTTCATGTTCCGTTGTTC
GTACACCCTGAACCCAGGAGAGTCCTCATAATAGGTGGAGGGGATGGAGGAAGTCTGCGTGAGGCTTTGAAGCATCCATC
TATTGAAGAGGCAATACTTTGTGAAATCGATCCTTATGTTATAGAAGCTGCAAAGAAATACTTGCCAACGGCTGTAGAAA
TGGACAACCCAAAAGCCAAAATCGTAAACGAAAATGGTGCTGAATTCATCAAGAAGTACAAGGATTATTTTGATGCCATA
ATCGTTGATTCCACCGATCCGACGGCTGGGGAGGGTGGCCATCTTTTCACAAAAGAATTCTACAAAAATTGTTTTGAAGC
GTTAAAGGAAGACGGTGTTTTCTCTGCCGAAACGGAAGATCCTTTCTACGATAAAGGATGGGTGGCCATAGCGTACAACA
GAATTTCATCTGCTTTCCCGATTTCTAAATTATATACAGGCTTTATGACAACCTATCCATCAGGACTGTGGACATATGTT
TTCGCTTCCAAAAAGTACCACCCAATTGACGACTTTGATCCGTCGAGAGTGCAGAATTTTGATCATCAATTAAAATATTA
CAACGAAGACATTCACAAGGCTTGCTTTGTCTTGCCGAATTTTATAAAAGAGTTGATTAACGAATAA

Upstream 100 bases:

>100_bases
ATGCAAAAAAAACCAGCGTGGTTGAACACCTTAGAGGAATTTACTCCGAAATTGGGATTCCTGAAACTGCACCACACAAG
GTATAACGGAGGGGATTGGT

Downstream 100 bases:

>100_bases
AAACACGAGAATCCAAGAAATGGTAAGCTCGATTACGCTGAGGGTACGAATCCCTTTGGGATTGACTCGAACTCCTGCGG
AGTTGTTCCGAACCACTTCG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKKDLRTMDHLMYMEYYTGRNAGIFMKIKKMLHSYQSPYQRIDIFEHPDFGKVFALDGITMMTEVDEFMYHEMLVHVPLF
VHPEPRRVLIIGGGDGGSLREALKHPSIEEAILCEIDPYVIEAAKKYLPTAVEMDNPKAKIVNENGAEFIKKYKDYFDAI
IVDSTDPTAGEGGHLFTKEFYKNCFEALKEDGVFSAETEDPFYDKGWVAIAYNRISSAFPISKLYTGFMTTYPSGLWTYV
FASKKYHPIDDFDPSRVQNFDHQLKYYNEDIHKACFVLPNFIKELINE

Sequences:

>Translated_288_residues
MKKDLRTMDHLMYMEYYTGRNAGIFMKIKKMLHSYQSPYQRIDIFEHPDFGKVFALDGITMMTEVDEFMYHEMLVHVPLF
VHPEPRRVLIIGGGDGGSLREALKHPSIEEAILCEIDPYVIEAAKKYLPTAVEMDNPKAKIVNENGAEFIKKYKDYFDAI
IVDSTDPTAGEGGHLFTKEFYKNCFEALKEDGVFSAETEDPFYDKGWVAIAYNRISSAFPISKLYTGFMTTYPSGLWTYV
FASKKYHPIDDFDPSRVQNFDHQLKYYNEDIHKACFVLPNFIKELINE
>Mature_288_residues
MKKDLRTMDHLMYMEYYTGRNAGIFMKIKKMLHSYQSPYQRIDIFEHPDFGKVFALDGITMMTEVDEFMYHEMLVHVPLF
VHPEPRRVLIIGGGDGGSLREALKHPSIEEAILCEIDPYVIEAAKKYLPTAVEMDNPKAKIVNENGAEFIKKYKDYFDAI
IVDSTDPTAGEGGHLFTKEFYKNCFEALKEDGVFSAETEDPFYDKGWVAIAYNRISSAFPISKLYTGFMTTYPSGLWTYV
FASKKYHPIDDFDPSRVQNFDHQLKYYNEDIHKACFVLPNFIKELINE

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=272, Percent_Identity=39.7058823529412, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI1786312, Length=269, Percent_Identity=35.3159851301115, Blast_Score=179, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI25154106, Length=268, Percent_Identity=42.5373134328358, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI212645616, Length=220, Percent_Identity=45, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6325326, Length=271, Percent_Identity=37.2693726937269, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323175, Length=276, Percent_Identity=34.4202898550725, Blast_Score=158, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24645443, Length=263, Percent_Identity=34.9809885931559, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21355941, Length=216, Percent_Identity=33.7962962962963, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 33369; Mature: 33369

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDLRTMDHLMYMEYYTGRNAGIFMKIKKMLHSYQSPYQRIDIFEHPDFGKVFALDGIT
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEECCCH
MMTEVDEFMYHEMLVHVPLFVHPEPRRVLIIGGGDGGSLREALKHPSIEEAILCEIDPYV
HHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHCCCCCCCEEEECCCHHH
IEAAKKYLPTAVEMDNPKAKIVNENGAEFIKKYKDYFDAIIVDSTDPTAGEGGHLFTKEF
HHHHHHHCCCEEECCCCCEEEECCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCCHHHHH
YKNCFEALKEDGVFSAETEDPFYDKGWVAIAYNRISSAFPISKLYTGFMTTYPSGLWTYV
HHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCCCCEEEE
FASKKYHPIDDFDPSRVQNFDHQLKYYNEDIHKACFVLPNFIKELINE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKDLRTMDHLMYMEYYTGRNAGIFMKIKKMLHSYQSPYQRIDIFEHPDFGKVFALDGIT
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEECCCH
MMTEVDEFMYHEMLVHVPLFVHPEPRRVLIIGGGDGGSLREALKHPSIEEAILCEIDPYV
HHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHCCCCCCCEEEECCCHHH
IEAAKKYLPTAVEMDNPKAKIVNENGAEFIKKYKDYFDAIIVDSTDPTAGEGGHLFTKEF
HHHHHHHCCCEEECCCCCEEEECCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCCHHHHH
YKNCFEALKEDGVFSAETEDPFYDKGWVAIAYNRISSAFPISKLYTGFMTTYPSGLWTYV
HHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCCCCEEEE
FASKKYHPIDDFDPSRVQNFDHQLKYYNEDIHKACFVLPNFIKELINE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]