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The map label for this gene is ispH

Identifier: 239616620

GI number: 239616620

Start: 242006

End: 242902

Strand: Direct

Name: ispH

Synonym: Kole_0213

Alternate gene names: 239616620

Gene position: 242006-242902 (Clockwise)

Preceding gene: 239616619

Following gene: 239616621

Centisome position: 10.51

GC content: 40.8

Gene sequence:

>897_bases
ATGAAGATCGTTCTCGCTGAATCACTGGGTTTTTGTTACGGCGTGAATAGAGCCGTTGAAGCGACGAAAAGGCTTTTGAA
GGAAAAACGCGATGCCCGGGTAATCGGTGATATTGTTCACAATGAAATTGTGATGAAAAAACTTAAAGATATGGGATTGA
AGGTTTACCCGGATCTTTCTTCAATCGATTTCTCTGATACAGCTGAGAATTCCGTGGCGATAATCAGGGCTCACGGCGCT
TCACCCGATGTTGAGGATTTTCTAAAAAGGCGTTTTGGGTATGTTGTGGATTTAACCTGCCCGATAGTGTACAATGTATT
TAGTTTGGCGAAAGACCTTGAAAAAAAAGGTTATTTTATAGTTATATTCGGAAAAAAAGACCACGCAGAGGTTAAAGCCC
TGTGTGGAAGGCTAAATGATTACCTGATCGTTGAACCGGGTTATGATTTTGAAACCATAAAGGCTTTTTTAAGAGAAAAA
AAGCCCAAGAAAGTTGCCCTGATTTCCCAAACAACCATGAACAGCGAGAGTTTTGAAAAATTGGCAGAAAAGCTGTTAAA
ATTGGAAGGCATCGAAGTTTCTGTGAATAACACCATCTGCAATGTAACCATAAACCGCGAAAAGATGGCGATTCACTTAG
CACAAACCTGTGATGCTGTGGTGGTTGTTGGTGGTAAAAGAAGTTCAAATACAGGGAAGCTCGCGAAGATTGTCGAAAGC
CATGGCAAAAGGGCTTTTCACGTTCAGAATCCGGAGCAGCTTCCGGATTTGTCTATTTATAGAAAAGTCGGTATCATAAG
CGGAACATCAACACCGAAGGAACAAATCCTGAAAATCTTGGATTACATAAAATTTACCTATGAAGGGGAGGTTATCCGTA
ATGGAGGAGAAGTATAA

Upstream 100 bases:

>100_bases
CTTTAAAACCCGCGAAAGACGCTATCATAATCGACACAACGAATATGACAATAGACGAGGTTCTCGATCTGCTTGTTAGA
AAGGTCAGGGATAAAGGTAC

Downstream 100 bases:

>100_bases
CGGACAGAACGAAGAAAACCTCAGCATGGAGGATATTCTGAAACAGATGGATGAGGTTGATGAAAGAAGGGGGAGAAGCA
AAACCGCTGAAGTTCATTCG

Product: hydroxymethylbutenyl pyrophosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKIVLAESLGFCYGVNRAVEATKRLLKEKRDARVIGDIVHNEIVMKKLKDMGLKVYPDLSSIDFSDTAENSVAIIRAHGA
SPDVEDFLKRRFGYVVDLTCPIVYNVFSLAKDLEKKGYFIVIFGKKDHAEVKALCGRLNDYLIVEPGYDFETIKAFLREK
KPKKVALISQTTMNSESFEKLAEKLLKLEGIEVSVNNTICNVTINREKMAIHLAQTCDAVVVVGGKRSSNTGKLAKIVES
HGKRAFHVQNPEQLPDLSIYRKVGIISGTSTPKEQILKILDYIKFTYEGEVIRNGGEV

Sequences:

>Translated_298_residues
MKIVLAESLGFCYGVNRAVEATKRLLKEKRDARVIGDIVHNEIVMKKLKDMGLKVYPDLSSIDFSDTAENSVAIIRAHGA
SPDVEDFLKRRFGYVVDLTCPIVYNVFSLAKDLEKKGYFIVIFGKKDHAEVKALCGRLNDYLIVEPGYDFETIKAFLREK
KPKKVALISQTTMNSESFEKLAEKLLKLEGIEVSVNNTICNVTINREKMAIHLAQTCDAVVVVGGKRSSNTGKLAKIVES
HGKRAFHVQNPEQLPDLSIYRKVGIISGTSTPKEQILKILDYIKFTYEGEVIRNGGEV
>Mature_298_residues
MKIVLAESLGFCYGVNRAVEATKRLLKEKRDARVIGDIVHNEIVMKKLKDMGLKVYPDLSSIDFSDTAENSVAIIRAHGA
SPDVEDFLKRRFGYVVDLTCPIVYNVFSLAKDLEKKGYFIVIFGKKDHAEVKALCGRLNDYLIVEPGYDFETIKAFLREK
KPKKVALISQTTMNSESFEKLAEKLLKLEGIEVSVNNTICNVTINREKMAIHLAQTCDAVVVVGGKRSSNTGKLAKIVES
HGKRAFHVQNPEQLPDLSIYRKVGIISGTSTPKEQILKILDYIKFTYEGEVIRNGGEV

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=304, Percent_Identity=27.6315789473684, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_KOSOT (C5CIU9)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002939942.1
- GeneID:   7968365
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_0213
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 33343; Mature: 33343

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVLAESLGFCYGVNRAVEATKRLLKEKRDARVIGDIVHNEIVMKKLKDMGLKVYPDLS
CEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCC
SIDFSDTAENSVAIIRAHGASPDVEDFLKRRFGYVVDLTCPIVYNVFSLAKDLEKKGYFI
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCEE
VIFGKKDHAEVKALCGRLNDYLIVEPGYDFETIKAFLREKKPKKVALISQTTMNSESFEK
EEECCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
LAEKLLKLEGIEVSVNNTICNVTINREKMAIHLAQTCDAVVVVGGKRSSNTGKLAKIVES
HHHHHHHHCCEEEEECCEEEEEEECCHHEEEEEECCCCEEEEECCCCCCCCHHHHHHHHH
HGKRAFHVQNPEQLPDLSIYRKVGIISGTSTPKEQILKILDYIKFTYEGEVIRNGGEV
CCCEEEECCCHHHCCCHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCC
>Mature Secondary Structure
MKIVLAESLGFCYGVNRAVEATKRLLKEKRDARVIGDIVHNEIVMKKLKDMGLKVYPDLS
CEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCC
SIDFSDTAENSVAIIRAHGASPDVEDFLKRRFGYVVDLTCPIVYNVFSLAKDLEKKGYFI
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCEE
VIFGKKDHAEVKALCGRLNDYLIVEPGYDFETIKAFLREKKPKKVALISQTTMNSESFEK
EEECCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
LAEKLLKLEGIEVSVNNTICNVTINREKMAIHLAQTCDAVVVVGGKRSSNTGKLAKIVES
HHHHHHHHCCEEEEECCEEEEEEECCHHEEEEEECCCCEEEEECCCCCCCCHHHHHHHHH
HGKRAFHVQNPEQLPDLSIYRKVGIISGTSTPKEQILKILDYIKFTYEGEVIRNGGEV
CCCEEEECCCHHHCCCHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA