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The map label for this gene is aspS [H]

Identifier: 239616618

GI number: 239616618

Start: 239587

End: 241362

Strand: Direct

Name: aspS [H]

Synonym: Kole_0211

Alternate gene names: 239616618

Gene position: 239587-241362 (Clockwise)

Preceding gene: 239616605

Following gene: 239616619

Centisome position: 10.41

GC content: 46.85

Gene sequence:

>1776_bases
TTGAAGAAGAGAACACATACCTGCGGAGAGTTGAGAAGTGAACACGCTGGAAAAACCGTCGTGTTGAACGGCTGGGTTGA
TAGGATCAGAGACCTCGGCGGCATAAAGTTCATTTTGCTCAGGGACCGTTACGGTTTCACTCAGGTCGTTTTTGATCCAT
CCAACGAGGATGTCTATAAGACGGCGCTGCAACTCGGTATCGAATATACTATATCTGTAACGGGCATCGTACGTGAGAGA
CCGAAGGATGCTGTTAACCCGAAGATGCCAACAGGCAAGATAGAGGTTCTGGCTACCGAAGTCGAGATCCTTTCGGAATC
AGATACACCACCCATATACGTTAATATCGATGATGACTCATCTGAAGAGCTAAAACTCAAATACCGATATCTTAATTTGA
GAAAGCCGAAGATGCAGAGAAATATAATCTTCAGGCATAAAGTGGTTCAGGCGGTCCGAAATTATCTCAACAAGAACAAT
TTTCTCGAGATAGAAACACCGGTCCTAACAAAATCAACTCCAGAAGGCGCAAGGGATTTTCTGGTCCCTTCGAGGTTAAG
GCCCGGCAAGTTTTATGCTTTACCCCAATCACCCCAACTTTTCAAACAACTCCTGATGGTTGCGGGTTTTGACAGGTATT
ACCAGATTGCTAAATGTTACCGCGATGAGGATTTCAGAGCTGACAGGCAACCGGAATTCACCCAGATAGATCTTGAGATG
TCCTTTGCCGACAGCGAAGACGTCTTTGCCATCGTTGAAGGTATGCTTTCCCACACCTTCAAAGAAACTCTGAATGTTGG
GCTAACGACACCTTTCCCCAGGTTAACGTATGAAGAAGCCATGGAAATGTACGGCTCCGATAGACCCGACAGGCGTTACG
GAATGGAGCTTCACGATGTGTCTGAAGCCTTTGTCGGAACCGGGTTCAGGGTTATATCGGAAGCCCTGGGACGCGGCGAG
ATCGTTAAAGGATTTCTCGCAAAGGGGCTTTCATCGAAGTTCTCGCGAAAGAGGCTGGACGAATACACGGCTTTTGTAAA
AGAAAACGGACTTGGCGGATTGATATGGATAAAGGTTGAAGACGAACTGAGATCGAGCATATTAAAACACTGCCCCGATG
AAATAAAGAAAGTTGTTGAGCGTTTCAATGCGAAAATTGGGGACATAATCTTCCTCGCTATTGGAAAGAGAGAAACTGTG
TCTAAAGCGCTGGGCAAGCTGCGAGAAAAACTGATCTCTGAGGAAAAACCGGAGGTTACAGGCTTTGACATACTCTGGGT
AACCGATTTTCCAATGTTCTCTTACAACGAGGAAGAAGGCAGGATAGAAGCGGAGCACCATCCCTTCACAATGCCGAACC
TTGGAGACCTGGACAAATTCGGTAAAACTGAGCCATTGAAGGTCAGGTCTCACGCTTACGACCTGGTGATAAATGGCTAC
GAACTGGCAAGCGGAAGCGTCAGAATTCACCGTAGGGATATCCAGAACAGGATATTCGAGATGATCGGTTTAACCCACGA
GGAAGCTCAAGAAAAATTCGGGTTTCTTCTTGAAGCCTTTAAATATGGCCCACCACCACACGCCGGCATCGCGTTCGGCC
TGGACAGATTGGTGGCTATAATGGTTGGAACCAGTTCCATACGCGATGTTATCGCTTTCCCGAAAGCGGCAAGCGGTAAC
GATCCTATGACCGGAGCCCCATCAGAGGTATCAGAACGTCAACTTGAGGAACTGAGAATCAAGATAATCGATGAAAAGGA
TGGGAAGAAAAGATGA

Upstream 100 bases:

>100_bases
CATTCACCTCCCAATGGTATATTCGCTTAAAATTCTACCATAAAACGTCACGCCTATTGTGGCCAATCGTGGTAATATTT
AAAATCAGGAGGTGGCAACA

Downstream 100 bases:

>100_bases
AGATCGCAATTGATGGGCCCGCCGGTTCCGGAAAATCAACCGTCGCAAAGATGCTTGCTGAACGGTTGGGATTCACCTAT
GTGGATACGGGCGCTATGTA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MKKRTHTCGELRSEHAGKTVVLNGWVDRIRDLGGIKFILLRDRYGFTQVVFDPSNEDVYKTALQLGIEYTISVTGIVRER
PKDAVNPKMPTGKIEVLATEVEILSESDTPPIYVNIDDDSSEELKLKYRYLNLRKPKMQRNIIFRHKVVQAVRNYLNKNN
FLEIETPVLTKSTPEGARDFLVPSRLRPGKFYALPQSPQLFKQLLMVAGFDRYYQIAKCYRDEDFRADRQPEFTQIDLEM
SFADSEDVFAIVEGMLSHTFKETLNVGLTTPFPRLTYEEAMEMYGSDRPDRRYGMELHDVSEAFVGTGFRVISEALGRGE
IVKGFLAKGLSSKFSRKRLDEYTAFVKENGLGGLIWIKVEDELRSSILKHCPDEIKKVVERFNAKIGDIIFLAIGKRETV
SKALGKLREKLISEEKPEVTGFDILWVTDFPMFSYNEEEGRIEAEHHPFTMPNLGDLDKFGKTEPLKVRSHAYDLVINGY
ELASGSVRIHRRDIQNRIFEMIGLTHEEAQEKFGFLLEAFKYGPPPHAGIAFGLDRLVAIMVGTSSIRDVIAFPKAASGN
DPMTGAPSEVSERQLEELRIKIIDEKDGKKR

Sequences:

>Translated_591_residues
MKKRTHTCGELRSEHAGKTVVLNGWVDRIRDLGGIKFILLRDRYGFTQVVFDPSNEDVYKTALQLGIEYTISVTGIVRER
PKDAVNPKMPTGKIEVLATEVEILSESDTPPIYVNIDDDSSEELKLKYRYLNLRKPKMQRNIIFRHKVVQAVRNYLNKNN
FLEIETPVLTKSTPEGARDFLVPSRLRPGKFYALPQSPQLFKQLLMVAGFDRYYQIAKCYRDEDFRADRQPEFTQIDLEM
SFADSEDVFAIVEGMLSHTFKETLNVGLTTPFPRLTYEEAMEMYGSDRPDRRYGMELHDVSEAFVGTGFRVISEALGRGE
IVKGFLAKGLSSKFSRKRLDEYTAFVKENGLGGLIWIKVEDELRSSILKHCPDEIKKVVERFNAKIGDIIFLAIGKRETV
SKALGKLREKLISEEKPEVTGFDILWVTDFPMFSYNEEEGRIEAEHHPFTMPNLGDLDKFGKTEPLKVRSHAYDLVINGY
ELASGSVRIHRRDIQNRIFEMIGLTHEEAQEKFGFLLEAFKYGPPPHAGIAFGLDRLVAIMVGTSSIRDVIAFPKAASGN
DPMTGAPSEVSERQLEELRIKIIDEKDGKKR
>Mature_591_residues
MKKRTHTCGELRSEHAGKTVVLNGWVDRIRDLGGIKFILLRDRYGFTQVVFDPSNEDVYKTALQLGIEYTISVTGIVRER
PKDAVNPKMPTGKIEVLATEVEILSESDTPPIYVNIDDDSSEELKLKYRYLNLRKPKMQRNIIFRHKVVQAVRNYLNKNN
FLEIETPVLTKSTPEGARDFLVPSRLRPGKFYALPQSPQLFKQLLMVAGFDRYYQIAKCYRDEDFRADRQPEFTQIDLEM
SFADSEDVFAIVEGMLSHTFKETLNVGLTTPFPRLTYEEAMEMYGSDRPDRRYGMELHDVSEAFVGTGFRVISEALGRGE
IVKGFLAKGLSSKFSRKRLDEYTAFVKENGLGGLIWIKVEDELRSSILKHCPDEIKKVVERFNAKIGDIIFLAIGKRETV
SKALGKLREKLISEEKPEVTGFDILWVTDFPMFSYNEEEGRIEAEHHPFTMPNLGDLDKFGKTEPLKVRSHAYDLVINGY
ELASGSVRIHRRDIQNRIFEMIGLTHEEAQEKFGFLLEAFKYGPPPHAGIAFGLDRLVAIMVGTSSIRDVIAFPKAASGN
DPMTGAPSEVSERQLEELRIKIIDEKDGKKR

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=600, Percent_Identity=40, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI194272210, Length=220, Percent_Identity=28.1818181818182, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI5031815, Length=220, Percent_Identity=28.1818181818182, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI45439306, Length=329, Percent_Identity=24.0121580547112, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4758762, Length=283, Percent_Identity=24.0282685512367, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788173, Length=595, Percent_Identity=47.3949579831933, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI1790571, Length=347, Percent_Identity=25.6484149855908, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789256, Length=307, Percent_Identity=25.7328990228013, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787161, Length=267, Percent_Identity=25.8426966292135, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=591, Percent_Identity=36.2098138747885, Blast_Score=347, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI71994340, Length=190, Percent_Identity=30, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=190, Percent_Identity=30, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=190, Percent_Identity=30, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71984122, Length=290, Percent_Identity=25.5172413793103, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=280, Percent_Identity=26.0714285714286, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=652, Percent_Identity=36.0429447852761, Blast_Score=311, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6323011, Length=315, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=287, Percent_Identity=23.6933797909408, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=198, Percent_Identity=29.2929292929293, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24584738, Length=603, Percent_Identity=40.2985074626866, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24640851, Length=179, Percent_Identity=29.0502793296089, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640849, Length=179, Percent_Identity=29.0502793296089, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136276, Length=252, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921528, Length=279, Percent_Identity=24.7311827956989, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67412; Mature: 67412

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRTHTCGELRSEHAGKTVVLNGWVDRIRDLGGIKFILLRDRYGFTQVVFDPSNEDVYK
CCCCCCCHHHHHHHCCCCEEEEECHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCHHHH
TALQLGIEYTISVTGIVRERPKDAVNPKMPTGKIEVLATEVEILSESDTPPIYVNIDDDS
HHHHHCCEEEEEEEEHHHHCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCEEEEECCCC
SEELKLKYRYLNLRKPKMQRNIIFRHKVVQAVRNYLNKNNFLEIETPVLTKSTPEGARDF
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCCHHHC
LVPSRLRPGKFYALPQSPQLFKQLLMVAGFDRYYQIAKCYRDEDFRADRQPEFTQIDLEM
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFADSEDVFAIVEGMLSHTFKETLNVGLTTPFPRLTYEEAMEMYGSDRPDRRYGMELHDV
ECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCHHHH
SEAFVGTGFRVISEALGRGEIVKGFLAKGLSSKFSRKRLDEYTAFVKENGLGGLIWIKVE
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
DELRSSILKHCPDEIKKVVERFNAKIGDIIFLAIGKRETVSKALGKLREKLISEEKPEVT
HHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCC
GFDILWVTDFPMFSYNEEEGRIEAEHHPFTMPNLGDLDKFGKTEPLKVRSHAYDLVINGY
CEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCEEECCCEEEEEEECE
ELASGSVRIHRRDIQNRIFEMIGLTHEEAQEKFGFLLEAFKYGPPPHAGIAFGLDRLVAI
EECCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
MVGTSSIRDVIAFPKAASGNDPMTGAPSEVSERQLEELRIKIIDEKDGKKR
HHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCC
>Mature Secondary Structure
MKKRTHTCGELRSEHAGKTVVLNGWVDRIRDLGGIKFILLRDRYGFTQVVFDPSNEDVYK
CCCCCCCHHHHHHHCCCCEEEEECHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCHHHH
TALQLGIEYTISVTGIVRERPKDAVNPKMPTGKIEVLATEVEILSESDTPPIYVNIDDDS
HHHHHCCEEEEEEEEHHHHCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCEEEEECCCC
SEELKLKYRYLNLRKPKMQRNIIFRHKVVQAVRNYLNKNNFLEIETPVLTKSTPEGARDF
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCCHHHC
LVPSRLRPGKFYALPQSPQLFKQLLMVAGFDRYYQIAKCYRDEDFRADRQPEFTQIDLEM
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFADSEDVFAIVEGMLSHTFKETLNVGLTTPFPRLTYEEAMEMYGSDRPDRRYGMELHDV
ECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCHHHH
SEAFVGTGFRVISEALGRGEIVKGFLAKGLSSKFSRKRLDEYTAFVKENGLGGLIWIKVE
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
DELRSSILKHCPDEIKKVVERFNAKIGDIIFLAIGKRETVSKALGKLREKLISEEKPEVT
HHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCC
GFDILWVTDFPMFSYNEEEGRIEAEHHPFTMPNLGDLDKFGKTEPLKVRSHAYDLVINGY
CEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCEEECCCEEEEEEECE
ELASGSVRIHRRDIQNRIFEMIGLTHEEAQEKFGFLLEAFKYGPPPHAGIAFGLDRLVAI
EECCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
MVGTSSIRDVIAFPKAASGNDPMTGAPSEVSERQLEELRIKIIDEKDGKKR
HHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA