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The map label for this gene is dpnA [H]

Identifier: 239616524

GI number: 239616524

Start: 131539

End: 132291

Strand: Direct

Name: dpnA [H]

Synonym: Kole_0116

Alternate gene names: 239616524

Gene position: 131539-132291 (Clockwise)

Preceding gene: 239616522

Following gene: 239616525

Centisome position: 5.71

GC content: 34.13

Gene sequence:

>753_bases
ATGCTTAATATAAAACTTATTTTAGGGGACTGCATAGAGGAACTAAAAAAACTCGAAGCTGAAAGTGTAGATTTAATATT
TGCGGATCCACCTTATAATCTATCAGGCGAAAACCATCTTACTGTACATGCAGGTAAACCAGTAAAACTCGATAAAGGCG
AGTGGGATAAAATCGATAATATTCACGAATTTAATTTGAATTGGATAAAAGAATGTATCAGAGTTTTAAAGCCTCATGGA
ACAATATGGATCTCAGGGACATTACATAATCATCCTTCCATAGGAATGGCCCTTAAACAACTTGGTTTATGGATAATTAA
TGATGTGGTCTGGTATAAACCAAATGCCACCCCACTTTTATCAAAAAACAGATTCGTTCCCTCAACTGAGATCATTTGGG
TTGCAGGTAAAAGTAAGAAGTATTACTTCAACTATGAATTAGCAAAAGAAATGAACAAAGGAAAACAAATGAGAAATTTA
TGGACAATAAAAGCAAAAAGACATATAACTCCCCACCCCGCAGAAAAACCAGAAGAACTGCTGGAAAGAATAATCCTGAT
TGGTAGCAATGAAGCAGATACAATCCTGGATCCGTTTATGGGTTCAGGCACAACAGGTGTTGTAGCTAAAAGATATAATA
GAAACTTCATAGGAATAGAAATTAATCAAGAATATTTTGAATGGGCAAAAGAAAGAATTGAAAAAACGATCGTAAAACCA
TCTTTATTCGGGTCTATTAAAAAATCTGTGTAA

Upstream 100 bases:

>100_bases
GGTGTTGTGGCTGAGAGATTGAATCGTAAATGGATAGGCATTGAAATAAATGAGAAATTTATTAAAGTTGCAAAAAATAG
AGTTTACGGAGGCATAGGAC

Downstream 100 bases:

>100_bases
ACTCTCTTTGTCCCGAGATTTGGGATTGGAGCGAACTCCTGGTAAGAATCCGAGAGTCCGAGAATCGAGAGGGCCGCTGT
GCGGCGGATACGAATCCCTT

Product: DNA methylase N-4/N-6 domain protein

Products: NA

Alternate protein names: M.DpnIIB; Adenine-specific methyltransferase DpnIIB; M.DpnII 2 [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MLNIKLILGDCIEELKKLEAESVDLIFADPPYNLSGENHLTVHAGKPVKLDKGEWDKIDNIHEFNLNWIKECIRVLKPHG
TIWISGTLHNHPSIGMALKQLGLWIINDVVWYKPNATPLLSKNRFVPSTEIIWVAGKSKKYYFNYELAKEMNKGKQMRNL
WTIKAKRHITPHPAEKPEELLERIILIGSNEADTILDPFMGSGTTGVVAKRYNRNFIGIEINQEYFEWAKERIEKTIVKP
SLFGSIKKSV

Sequences:

>Translated_250_residues
MLNIKLILGDCIEELKKLEAESVDLIFADPPYNLSGENHLTVHAGKPVKLDKGEWDKIDNIHEFNLNWIKECIRVLKPHG
TIWISGTLHNHPSIGMALKQLGLWIINDVVWYKPNATPLLSKNRFVPSTEIIWVAGKSKKYYFNYELAKEMNKGKQMRNL
WTIKAKRHITPHPAEKPEELLERIILIGSNEADTILDPFMGSGTTGVVAKRYNRNFIGIEINQEYFEWAKERIEKTIVKP
SLFGSIKKSV
>Mature_250_residues
MLNIKLILGDCIEELKKLEAESVDLIFADPPYNLSGENHLTVHAGKPVKLDKGEWDKIDNIHEFNLNWIKECIRVLKPHG
TIWISGTLHNHPSIGMALKQLGLWIINDVVWYKPNATPLLSKNRFVPSTEIIWVAGKSKKYYFNYELAKEMNKGKQMRNL
WTIKAKRHITPHPAEKPEELLERIILIGSNEADTILDPFMGSGTTGVVAKRYNRNFIGIEINQEYFEWAKERIEKTIVKP
SLFGSIKKSV

Specific function: Essential for establishment of a previously unmethylated plasmid transformed into the cell as single stranded DNA. Recognizes the single- or double-stranded sequence GATC, causes specific methylation on A-2 on one or both strands (respectively), and prote

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=253, Percent_Identity=35.1778656126482, Blast_Score=126, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 28715; Mature: 28715

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNIKLILGDCIEELKKLEAESVDLIFADPPYNLSGENHLTVHAGKPVKLDKGEWDKIDN
CEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCC
IHEFNLNWIKECIRVLKPHGTIWISGTLHNHPSIGMALKQLGLWIINDVVWYKPNATPLL
EEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHEECCEEEECCCCCEEC
SKNRFVPSTEIIWVAGKSKKYYFNYELAKEMNKGKQMRNLWTIKAKRHITPHPAEKPEEL
CCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHCCHHHHHEEEEEECCCCCCCCCCCHHHH
LERIILIGSNEADTILDPFMGSGTTGVVAKRYNRNFIGIEINQEYFEWAKERIEKTIVKP
HHHHEEECCCCCCHHHHHCCCCCCCCEEEHHCCCCEEEEEECHHHHHHHHHHHHHHHCCH
SLFGSIKKSV
HHHHHHHHCC
>Mature Secondary Structure
MLNIKLILGDCIEELKKLEAESVDLIFADPPYNLSGENHLTVHAGKPVKLDKGEWDKIDN
CEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCC
IHEFNLNWIKECIRVLKPHGTIWISGTLHNHPSIGMALKQLGLWIINDVVWYKPNATPLL
EEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHEECCEEEECCCCCEEC
SKNRFVPSTEIIWVAGKSKKYYFNYELAKEMNKGKQMRNLWTIKAKRHITPHPAEKPEEL
CCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHCCHHHHHEEEEEECCCCCCCCCCCHHHH
LERIILIGSNEADTILDPFMGSGTTGVVAKRYNRNFIGIEINQEYFEWAKERIEKTIVKP
HHHHEEECCCCCCHHHHHCCCCCCCCEEEHHCCCCEEEEEECHHHHHHHHHHHHHHHCCH
SLFGSIKKSV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019562; 10712690; 2824782 [H]