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The map label for this gene is holB [H]

Identifier: 238925618

GI number: 238925618

Start: 3109596

End: 3110585

Strand: Reverse

Name: holB [H]

Synonym: EUBREC_3274

Alternate gene names: 238925618

Gene position: 3110585-3109596 (Counterclockwise)

Preceding gene: 238925619

Following gene: 238925617

Centisome position: 90.17

GC content: 44.95

Gene sequence:

>990_bases
ATGGCAGGATTTAAGGATATAGTAGGAAATGAGCAGATAATAGAGCATCTGCAAAATGCCATATCAATGGGAAAGGTCTC
ACATGCATATATAATAAACGGACCACAGCTGTCCGGCAAAATGATGATAGCCGAGGCATTTGCCAGGGCACTGCAGTGTG
AGAAGGAGGGCACAGACGGTTGTGGAGAGTGCAAATCGTGCCATCAGGCAGATGACCACAATCATCCTGATATCATATAT
GTCAGTCATGAAAAGCCCAACAACATATCGGTTGACGACATCAGGACACAGCTCAACAACGATATAGTGATAAAGCCGTA
CAGCAGTAAATATAAGATTTACATAGTGGATGAGGCTGAAAAAATGAATCAGCAGGCGCAGAACGCGCTTTTAAAGACCA
TTGAGGAGCCACCGGCATATGCGGTCATCATGCTTCTCACCACCAATGCAGACAGCTTTCTGCAGACCATCAGGTCGCGC
TGCATCACACTCAATATGAAGTCGGTAAAGGACGAGGTGATAAAGGCATACCTGATGAACGAAAAGCATATTCCGGATTA
TCAGGCAGATATCAGTGCCGCATTTGCACAGGGCATCGTCGGAAAGGCTGTTAAGCTCGCCTCATCAGACGAGTTCAACG
AGCTAAAGGAGTCAGCACTGTCGCTTATAAAGCGCCTCGATGACATAGATCTCTACGAGCTGGGTGCAGCGGTAAAGCAG
ATAGCTGAGTATAAGCTTAAGGTGCAGGATTATTTTGACCTGATTATGGTGTGGTACAGAGATGTGCTGTATATGAAGGC
GACAAACGATGTTAACGGACTCATATTCAAGGATGAGGTCTATGATATAAAGAAACAGGCAGCAAAGCATTCCTATCATG
GAATCGAGGCAATACTTGAGGCACTTGAGAAGGCGAAGATAAGAATTAACGCAAACGTCAATTTTGACCTGGTAATTGAG
CTGCTGCTTATGACCATAAAGGAGAACTAA

Upstream 100 bases:

>100_bases
TGGCAAGAATCGGAGAGATACGTGGACTGTTGCTTGATATATTCACGTAGGATATAAAAGCTATAACAGACACAGGCAGA
CTAAAACGGAGGCAGCAGCT

Downstream 100 bases:

>100_bases
TCACATGACAAAAATAGTTGGTATCAGATTCAGGACAGCAGGCAAAATATATTACTTTGACCCGGCTGATTTCGACCTCG
ACATGGCAATGCATGTTATC

Product: DNA polymerase III, delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MAGFKDIVGNEQIIEHLQNAISMGKVSHAYIINGPQLSGKMMIAEAFARALQCEKEGTDGCGECKSCHQADDHNHPDIIY
VSHEKPNNISVDDIRTQLNNDIVIKPYSSKYKIYIVDEAEKMNQQAQNALLKTIEEPPAYAVIMLLTTNADSFLQTIRSR
CITLNMKSVKDEVIKAYLMNEKHIPDYQADISAAFAQGIVGKAVKLASSDEFNELKESALSLIKRLDDIDLYELGAAVKQ
IAEYKLKVQDYFDLIMVWYRDVLYMKATNDVNGLIFKDEVYDIKKQAAKHSYHGIEAILEALEKAKIRINANVNFDLVIE
LLLMTIKEN

Sequences:

>Translated_329_residues
MAGFKDIVGNEQIIEHLQNAISMGKVSHAYIINGPQLSGKMMIAEAFARALQCEKEGTDGCGECKSCHQADDHNHPDIIY
VSHEKPNNISVDDIRTQLNNDIVIKPYSSKYKIYIVDEAEKMNQQAQNALLKTIEEPPAYAVIMLLTTNADSFLQTIRSR
CITLNMKSVKDEVIKAYLMNEKHIPDYQADISAAFAQGIVGKAVKLASSDEFNELKESALSLIKRLDDIDLYELGAAVKQ
IAEYKLKVQDYFDLIMVWYRDVLYMKATNDVNGLIFKDEVYDIKKQAAKHSYHGIEAILEALEKAKIRINANVNFDLVIE
LLLMTIKEN
>Mature_328_residues
AGFKDIVGNEQIIEHLQNAISMGKVSHAYIINGPQLSGKMMIAEAFARALQCEKEGTDGCGECKSCHQADDHNHPDIIYV
SHEKPNNISVDDIRTQLNNDIVIKPYSSKYKIYIVDEAEKMNQQAQNALLKTIEEPPAYAVIMLLTTNADSFLQTIRSRC
ITLNMKSVKDEVIKAYLMNEKHIPDYQADISAAFAQGIVGKAVKLASSDEFNELKESALSLIKRLDDIDLYELGAAVKQI
AEYKLKVQDYFDLIMVWYRDVLYMKATNDVNGLIFKDEVYDIKKQAAKHSYHGIEAILEALEKAKIRINANVNFDLVIEL
LLMTIKEN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=185, Percent_Identity=34.0540540540541, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787341, Length=230, Percent_Identity=27.3913043478261, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37125; Mature: 36994

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGFKDIVGNEQIIEHLQNAISMGKVSHAYIINGPQLSGKMMIAEAFARALQCEKEGTDG
CCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCC
CGECKSCHQADDHNHPDIIYVSHEKPNNISVDDIRTQLNNDIVIKPYSSKYKIYIVDEAE
CHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEEECHH
KMNQQAQNALLKTIEEPPAYAVIMLLTTNADSFLQTIRSRCITLNMKSVKDEVIKAYLMN
HHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHEECCHHHHHHHHHHHHHHC
EKHIPDYQADISAAFAQGIVGKAVKLASSDEFNELKESALSLIKRLDDIDLYELGAAVKQ
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IAEYKLKVQDYFDLIMVWYRDVLYMKATNDVNGLIFKDEVYDIKKQAAKHSYHGIEAILE
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
ALEKAKIRINANVNFDLVIELLLMTIKEN
HHHHCEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGFKDIVGNEQIIEHLQNAISMGKVSHAYIINGPQLSGKMMIAEAFARALQCEKEGTDG
CCCHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCC
CGECKSCHQADDHNHPDIIYVSHEKPNNISVDDIRTQLNNDIVIKPYSSKYKIYIVDEAE
CHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEEECHH
KMNQQAQNALLKTIEEPPAYAVIMLLTTNADSFLQTIRSRCITLNMKSVKDEVIKAYLMN
HHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHEECCHHHHHHHHHHHHHHC
EKHIPDYQADISAAFAQGIVGKAVKLASSDEFNELKESALSLIKRLDDIDLYELGAAVKQ
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IAEYKLKVQDYFDLIMVWYRDVLYMKATNDVNGLIFKDEVYDIKKQAAKHSYHGIEAILE
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
ALEKAKIRINANVNFDLVIELLLMTIKEN
HHHHCEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]