Click here to switch to the map view.

The map label for this gene is cobB [H]

Identifier: 238925612

GI number: 238925612

Start: 3103912

End: 3104697

Strand: Reverse

Name: cobB [H]

Synonym: EUBREC_3268

Alternate gene names: 238925612

Gene position: 3104697-3103912 (Counterclockwise)

Preceding gene: 238925613

Following gene: 238925611

Centisome position: 90.0

GC content: 47.84

Gene sequence:

>786_bases
GTGGCTCAAAAAATAATAGGCAGCAATTTAAAGAAGAAAGGATGGACACATATGGGTAACAATGACAGCAGTGTAGAAAC
ATTTTTGCAGTGGATAAAGGCTTCGGACAATATTGTATTTTTCGGCGGAGCAGGTGTATCAACCGAGAGTGGCATACCTG
ATTTCAGAAGCGTGGACGGACTTTACAATCAGAAGTATGACTACCCGCCAGAGACCATCCTGAGCCATACATTTTACAGG
AAGCATCCGCAGGAGTTTTACCGATTCTACCGTGACAAGATGCTCTGCCTTGATGCAGAGCCGAACATCACGCATTACAA
GCTGGCAGAGCTTGAAAAAGCAGGAAAGCTTAAGGGCGTTGTGACGCAGAACATAGATGGACTGCACCAGAAGGCAGGCA
GCAAAAATGTGATGGAACTGCACGGCAGTGTGCTGAGAAACTACTGTGAGAGGTGCTTGCAGTTTGTAAGCGCAGAGGAA
ATCCTCAACTCGACAGACGTGCCAAAATGCCCAAAGTGCGGCGGTCCGGTAAAGCCGGATGTCGTATTGTACGAGGAGGG
CTTAAATCAGAAAACGCTTGAGGATGCAATCTACTACATCAGTCACGCCGATGTGCTCATAGTAGGCGGTACATCACTTG
CGGTATATCCGGCGGCAGGCCTCATAGACTATTACAATGGAAATAAGCTCGTGCTTATCAACAAGTCCACCACACCGATG
GACGCAAGAGCAGACCTTCTGATACAGCAGGGACTGGGCTCTGTATTTGAGCAGATAGAGGTGTAA

Upstream 100 bases:

>100_bases
GTGCTTCCGCTGCCGGACTACGTGTTCAAGGCAGACGATGCAGTGATGGTGCTTGGACTGAATGCAGATGTGGACAAGCT
GGTGCGCAAGCTATAAAAGT

Downstream 100 bases:

>100_bases
ACAGAGGATAATACACAGCATATTAAATGGCAGGCATAGGTAGAAGAGGAATATAGAATGGATAAATACGCAGTAGGAGA
TATCGTAAAACTGAAAAAAC

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MAQKIIGSNLKKKGWTHMGNNDSSVETFLQWIKASDNIVFFGGAGVSTESGIPDFRSVDGLYNQKYDYPPETILSHTFYR
KHPQEFYRFYRDKMLCLDAEPNITHYKLAELEKAGKLKGVVTQNIDGLHQKAGSKNVMELHGSVLRNYCERCLQFVSAEE
ILNSTDVPKCPKCGGPVKPDVVLYEEGLNQKTLEDAIYYISHADVLIVGGTSLAVYPAAGLIDYYNGNKLVLINKSTTPM
DARADLLIQQGLGSVFEQIEV

Sequences:

>Translated_261_residues
MAQKIIGSNLKKKGWTHMGNNDSSVETFLQWIKASDNIVFFGGAGVSTESGIPDFRSVDGLYNQKYDYPPETILSHTFYR
KHPQEFYRFYRDKMLCLDAEPNITHYKLAELEKAGKLKGVVTQNIDGLHQKAGSKNVMELHGSVLRNYCERCLQFVSAEE
ILNSTDVPKCPKCGGPVKPDVVLYEEGLNQKTLEDAIYYISHADVLIVGGTSLAVYPAAGLIDYYNGNKLVLINKSTTPM
DARADLLIQQGLGSVFEQIEV
>Mature_260_residues
AQKIIGSNLKKKGWTHMGNNDSSVETFLQWIKASDNIVFFGGAGVSTESGIPDFRSVDGLYNQKYDYPPETILSHTFYRK
HPQEFYRFYRDKMLCLDAEPNITHYKLAELEKAGKLKGVVTQNIDGLHQKAGSKNVMELHGSVLRNYCERCLQFVSAEEI
LNSTDVPKCPKCGGPVKPDVVLYEEGLNQKTLEDAIYYISHADVLIVGGTSLAVYPAAGLIDYYNGNKLVLINKSTTPMD
ARADLLIQQGLGSVFEQIEV

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=257, Percent_Identity=34.2412451361868, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI6912664, Length=271, Percent_Identity=32.8413284132841, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI6912660, Length=213, Percent_Identity=38.0281690140845, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI13787215, Length=254, Percent_Identity=33.4645669291339, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI63054862, Length=203, Percent_Identity=38.9162561576355, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI7657575, Length=233, Percent_Identity=33.0472103004292, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI6912662, Length=273, Percent_Identity=32.967032967033, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI13775602, Length=213, Percent_Identity=35.2112676056338, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI13775600, Length=213, Percent_Identity=35.2112676056338, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI300797705, Length=201, Percent_Identity=34.3283582089552, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI300797577, Length=241, Percent_Identity=29.045643153527, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI300797597, Length=226, Percent_Identity=30.5309734513274, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI215982798, Length=173, Percent_Identity=32.9479768786127, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI308199517, Length=191, Percent_Identity=35.0785340314136, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17541892, Length=244, Percent_Identity=31.5573770491803, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71990482, Length=278, Percent_Identity=29.4964028776978, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71990487, Length=280, Percent_Identity=29.2857142857143, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17567771, Length=275, Percent_Identity=30.5454545454545, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325242, Length=275, Percent_Identity=32, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320163, Length=256, Percent_Identity=26.953125, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324504, Length=256, Percent_Identity=26.171875, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320397, Length=259, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17137536, Length=237, Percent_Identity=31.6455696202532, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648389, Length=211, Percent_Identity=35.5450236966825, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28571445, Length=278, Percent_Identity=28.0575539568345, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24645650, Length=251, Percent_Identity=29.8804780876494, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28571443, Length=192, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571441, Length=192, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 29106; Mature: 28975

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKIIGSNLKKKGWTHMGNNDSSVETFLQWIKASDNIVFFGGAGVSTESGIPDFRSVDG
CCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHH
LYNQKYDYPPETILSHTFYRKHPQEFYRFYRDKMLCLDAEPNITHYKLAELEKAGKLKGV
HHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCHH
VTQNIDGLHQKAGSKNVMELHGSVLRNYCERCLQFVSAEEILNSTDVPKCPKCGGPVKPD
HHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VVLYEEGLNQKTLEDAIYYISHADVLIVGGTSLAVYPAAGLIDYYNGNKLVLINKSTTPM
EEEEECCCCHHHHHHHHHHHCCCCEEEECCCEEEEECCCCEEEEECCCEEEEEECCCCCC
DARADLLIQQGLGSVFEQIEV
CHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQKIIGSNLKKKGWTHMGNNDSSVETFLQWIKASDNIVFFGGAGVSTESGIPDFRSVDG
CCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHH
LYNQKYDYPPETILSHTFYRKHPQEFYRFYRDKMLCLDAEPNITHYKLAELEKAGKLKGV
HHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCHH
VTQNIDGLHQKAGSKNVMELHGSVLRNYCERCLQFVSAEEILNSTDVPKCPKCGGPVKPD
HHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VVLYEEGLNQKTLEDAIYYISHADVLIVGGTSLAVYPAAGLIDYYNGNKLVLINKSTTPM
EEEEECCCCHHHHHHHHHHHCCCCEEEECCCEEEEECCCCEEEEECCCEEEEEECCCCCC
DARADLLIQQGLGSVFEQIEV
CHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]