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The map label for this gene is galE [C]

Identifier: 238925593

GI number: 238925593

Start: 3087467

End: 3088510

Strand: Reverse

Name: galE [C]

Synonym: EUBREC_3249

Alternate gene names: 238925593

Gene position: 3088510-3087467 (Counterclockwise)

Preceding gene: 238925594

Following gene: 238925592

Centisome position: 89.53

GC content: 38.12

Gene sequence:

>1044_bases
ATGAAGGTATTCCAAGCATTGATAGAAAATATCAGGAGGAAATATTTCATGTTAAATGGAAAGTCGATTTTGGTAACAGG
GGGAACAGGTTCATTTGGACATCAGTTTGTTAGTTATGTGTTGGAGCATTATGAGCCTAAGAAAATAATAATATATTCAA
GAGACGAATATAAGCAGTTCGTAATGCAGAATGAATATAAGAAACATCCACAGGCTGATAAGCTGCGTTTCTTTATAGGA
GATGTAAGGGATAAAGAAAGACTTTACAGAGCTTTCGATGGTGTGGATTATGTGGTACATGCAGCAGCATTAAAGCAGGT
TCCATCATGCGAGTACAATCCGATGGAAGCTGTTAAGACAAATATCAATGGTGCTATGAATATAGTGGATGCAGCATTAG
ATTGTGGGGTAAAGCGTGTTGTTGCTCTTTCGACTGACAAGGCGGTTAATCCAATTAATCTTTATGGAGGTACAAAACTT
GTTTCTGATAAGCTGTTTATTGCAGCAAATGCATATGCAGGGGAAAAGGACGTCAATTTTTCAATCGTCAGATATGGTAA
TGTAGCAGGAAGCAGAGGCTCAGTTATTCCATTTTTCAGAAATATAGTTGCTAATGGTGGTACTTCTCTTCCAATAACAG
ACTATAGGATGACAAGATTCTGGATAAGCCTTGAGCAGGGAGTAGAACTTGTGATTAAGGCATTGTCAGAGGCAAAAGGA
GGAGAGACTTTTATCTCAAAGATTCCTTCATTTAAGATAACAGATTTGGCACAAGCTATATTACCGGGATGCGAGATGCC
GGAGGTTGGTATACGTGAAGGAGAGAAACTTCATGAAATAATGGTTACAAGAGAAGACTCAATGCTCACATATGAATATG
ATAAGCACTTCATCGTATATCCACATTTTGATTGGTGGGATACTAATAAGATACAGGTTGGAGGGAAAAAAGTAGAGCAG
GGATTTGAATATAGTTCGGGGACAAATACCCAGTGGCTCAGTGTTGATGAGATTAAAACACTGTTAAAAACGGGCAAAAT
GTAA

Upstream 100 bases:

>100_bases
GAGATTCATGAAGAATCGTTAAGGTGCATGGAGGCATAAATTTATATCGGATGAATTATTATGCCACTCGGACTATGTAC
GTTCGGGTGGCTTTTTATTT

Downstream 100 bases:

>100_bases
TAGGTATACCGAAGCTTTGGTTAGATGCTAAGAAATTTTATTTTAACCAATTTTTTATGGACTGAAATGATAAGGCGGGA
ATTCCATAATCAGATAAATA

Product: UDP-4-dehydro-6-deoxy-2-acetamido-D-glucose 4-reductase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKVFQALIENIRRKYFMLNGKSILVTGGTGSFGHQFVSYVLEHYEPKKIIIYSRDEYKQFVMQNEYKKHPQADKLRFFIG
DVRDKERLYRAFDGVDYVVHAAALKQVPSCEYNPMEAVKTNINGAMNIVDAALDCGVKRVVALSTDKAVNPINLYGGTKL
VSDKLFIAANAYAGEKDVNFSIVRYGNVAGSRGSVIPFFRNIVANGGTSLPITDYRMTRFWISLEQGVELVIKALSEAKG
GETFISKIPSFKITDLAQAILPGCEMPEVGIREGEKLHEIMVTREDSMLTYEYDKHFIVYPHFDWWDTNKIQVGGKKVEQ
GFEYSSGTNTQWLSVDEIKTLLKTGKM

Sequences:

>Translated_347_residues
MKVFQALIENIRRKYFMLNGKSILVTGGTGSFGHQFVSYVLEHYEPKKIIIYSRDEYKQFVMQNEYKKHPQADKLRFFIG
DVRDKERLYRAFDGVDYVVHAAALKQVPSCEYNPMEAVKTNINGAMNIVDAALDCGVKRVVALSTDKAVNPINLYGGTKL
VSDKLFIAANAYAGEKDVNFSIVRYGNVAGSRGSVIPFFRNIVANGGTSLPITDYRMTRFWISLEQGVELVIKALSEAKG
GETFISKIPSFKITDLAQAILPGCEMPEVGIREGEKLHEIMVTREDSMLTYEYDKHFIVYPHFDWWDTNKIQVGGKKVEQ
GFEYSSGTNTQWLSVDEIKTLLKTGKM
>Mature_347_residues
MKVFQALIENIRRKYFMLNGKSILVTGGTGSFGHQFVSYVLEHYEPKKIIIYSRDEYKQFVMQNEYKKHPQADKLRFFIG
DVRDKERLYRAFDGVDYVVHAAALKQVPSCEYNPMEAVKTNINGAMNIVDAALDCGVKRVVALSTDKAVNPINLYGGTKL
VSDKLFIAANAYAGEKDVNFSIVRYGNVAGSRGSVIPFFRNIVANGGTSLPITDYRMTRFWISLEQGVELVIKALSEAKG
GETFISKIPSFKITDLAQAILPGCEMPEVGIREGEKLHEIMVTREDSMLTYEYDKHFIVYPHFDWWDTNKIQVGGKKVEQ
GFEYSSGTNTQWLSVDEIKTLLKTGKM

Specific function: Galactose metabolism; third step. [C]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 39226; Mature: 39226

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFQALIENIRRKYFMLNGKSILVTGGTGSFGHQFVSYVLEHYEPKKIIIYSRDEYKQF
CHHHHHHHHHHHHHEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHH
VMQNEYKKHPQADKLRFFIGDVRDKERLYRAFDGVDYVVHAAALKQVPSCEYNPMEAVKT
HHHHHHHCCCCHHHHHEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHH
NINGAMNIVDAALDCGVKRVVALSTDKAVNPINLYGGTKLVSDKLFIAANAYAGEKDVNF
CCCHHHHHHHHHHHCCHHEEEEECCCCCCCCEEECCCCEEECCEEEEEEECCCCCCCCCE
SIVRYGNVAGSRGSVIPFFRNIVANGGTSLPITDYRMTRFWISLEQGVELVIKALSEAKG
EEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHEEEEHHHHHHHHHHHHHHCCC
GETFISKIPSFKITDLAQAILPGCEMPEVGIREGEKLHEIMVTREDSMLTYEYDKHFIVY
CHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEECCCCEEEEEECCEEEEE
PHFDWWDTNKIQVGGKKVEQGFEYSSGTNTQWLSVDEIKTLLKTGKM
CCCCCCCCCEEEECCHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MKVFQALIENIRRKYFMLNGKSILVTGGTGSFGHQFVSYVLEHYEPKKIIIYSRDEYKQF
CHHHHHHHHHHHHHEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHH
VMQNEYKKHPQADKLRFFIGDVRDKERLYRAFDGVDYVVHAAALKQVPSCEYNPMEAVKT
HHHHHHHCCCCHHHHHEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHH
NINGAMNIVDAALDCGVKRVVALSTDKAVNPINLYGGTKLVSDKLFIAANAYAGEKDVNF
CCCHHHHHHHHHHHCCHHEEEEECCCCCCCCEEECCCCEEECCEEEEEEECCCCCCCCCE
SIVRYGNVAGSRGSVIPFFRNIVANGGTSLPITDYRMTRFWISLEQGVELVIKALSEAKG
EEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHEEEEHHHHHHHHHHHHHHCCC
GETFISKIPSFKITDLAQAILPGCEMPEVGIREGEKLHEIMVTREDSMLTYEYDKHFIVY
CHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEECCCCEEEEEECCEEEEE
PHFDWWDTNKIQVGGKKVEQGFEYSSGTNTQWLSVDEIKTLLKTGKM
CCCCCCCCCEEEECCHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]