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The map label for this gene is rffG [C]

Identifier: 238925589

GI number: 238925589

Start: 3083501

End: 3084559

Strand: Reverse

Name: rffG [C]

Synonym: EUBREC_3245

Alternate gene names: 238925589

Gene position: 3084559-3083501 (Counterclockwise)

Preceding gene: 238925590

Following gene: 238925588

Centisome position: 89.42

GC content: 32.48

Gene sequence:

>1059_bases
ATGAATTATTTTAGAAAACTTATTCTTATAAAGGAGAAGAAGCTAATGAATATTTTAATTATAGGGGCATCAAGTTTCAT
TGGTGCTTATCAAGTTGAACAATTGATTAATACACCTGATTTTGTGGGGGGAGTAGTCGGAACGGGGAGAAATCCTCGAT
TTAGGGATTATTATGAAAGTATCGGTGTGCCTTATTATAATATTGATGTATGTGATTATGATTCTTTTAAGGTTTTAGAT
CAGTATGATTTTGATGCTGCCGTTTTATTCGCTACAATGATGCCTTCAAACATTCCTAAAGATGATGATTCGGATGATAC
AGCAGAATACTATAAAGTTAATGTACTGGGGACATTAAATGCACTAGAATATTGTAGGACTCGAGGCATAAATAAATTGA
TTTCATTCGGTACGCGTTTCGACTGCAGACTCTATGATTCTAATACGGTAATTACAGAGGAAACACCGCTAAATTATTCG
TTAACCGATGATCATGCTGCATTTGTGATGTCAAATAACGCAAAATGGGATGTCATGAGATACTACAATGAAAAATATGA
GATGAACAATATATACTTTAGAATACCAACAATTTTTGGGGTTGGACCACATGGGAGTTTTTATAAGGATGGAATTTATA
GAAAATCTGGCATACAAATTTTTATAGATAAAGCGGCAGCAGGTGAACCAATAGAGGTATATGGAAGCACGGATACTACA
AAAGATTTACTTTATGTAAAAGATTTAAATATCGGTGTTTTAAACGCATTAAAGATTCCAACATCTAAAGGCTTTTATAA
TATAGGATATGAACAGAATTTCCGTTTGATAGATATAGTAAAAGCAATTATTGAGGTCTTTTCAAACGACAATAACATTT
CAACTGTTATACAGCGTCCAGATATTCCTAATAATGGAAGTTTTCCTTTAATGGATATAACTAAACTCAAGAAGGATATT
GGGTTCGAACCGCATTATTCAAACATTGTAGATATGATGGAAGATTATAAATGTGAACTTGAAAGGGGGCTTTACACTAG
ACTTTTTAATGTTTATTAA

Upstream 100 bases:

>100_bases
GATAAGGCTTTATATTATATTGAAAATTATGATAATCGGATAAGACTTGCGCAAAATGCACACAACTTAATTGATGGCAA
CGGATGCCAAAAGATTATTG

Downstream 100 bases:

>100_bases
TAATCTTTAATACTTCATATAAGATGTTAGAGGGGAAATTCCTATTTCTGGAGGTTTTCTTGCGGCGAATAATCTGAGTT
GAAAAATAAAGATAAAGCTG

Product: UDP-glucose 4-epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NDP-Sugar Dehydratase Or Epimerase; UDP-Glucose 4-Epimerase; DTDP-Glucose 4 6-Dehydratase; Nucleoside-Diphosphate-Sugar Epimerase; DTDP Glucose 4 6-Dehydratase; NAD Dependent Epimerase/Dehydratase Family Protein; Nucleotide Sugar Epimerase

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNYFRKLILIKEKKLMNILIIGASSFIGAYQVEQLINTPDFVGGVVGTGRNPRFRDYYESIGVPYYNIDVCDYDSFKVLD
QYDFDAAVLFATMMPSNIPKDDDSDDTAEYYKVNVLGTLNALEYCRTRGINKLISFGTRFDCRLYDSNTVITEETPLNYS
LTDDHAAFVMSNNAKWDVMRYYNEKYEMNNIYFRIPTIFGVGPHGSFYKDGIYRKSGIQIFIDKAAAGEPIEVYGSTDTT
KDLLYVKDLNIGVLNALKIPTSKGFYNIGYEQNFRLIDIVKAIIEVFSNDNNISTVIQRPDIPNNGSFPLMDITKLKKDI
GFEPHYSNIVDMMEDYKCELERGLYTRLFNVY

Sequences:

>Translated_352_residues
MNYFRKLILIKEKKLMNILIIGASSFIGAYQVEQLINTPDFVGGVVGTGRNPRFRDYYESIGVPYYNIDVCDYDSFKVLD
QYDFDAAVLFATMMPSNIPKDDDSDDTAEYYKVNVLGTLNALEYCRTRGINKLISFGTRFDCRLYDSNTVITEETPLNYS
LTDDHAAFVMSNNAKWDVMRYYNEKYEMNNIYFRIPTIFGVGPHGSFYKDGIYRKSGIQIFIDKAAAGEPIEVYGSTDTT
KDLLYVKDLNIGVLNALKIPTSKGFYNIGYEQNFRLIDIVKAIIEVFSNDNNISTVIQRPDIPNNGSFPLMDITKLKKDI
GFEPHYSNIVDMMEDYKCELERGLYTRLFNVY
>Mature_352_residues
MNYFRKLILIKEKKLMNILIIGASSFIGAYQVEQLINTPDFVGGVVGTGRNPRFRDYYESIGVPYYNIDVCDYDSFKVLD
QYDFDAAVLFATMMPSNIPKDDDSDDTAEYYKVNVLGTLNALEYCRTRGINKLISFGTRFDCRLYDSNTVITEETPLNYS
LTDDHAAFVMSNNAKWDVMRYYNEKYEMNNIYFRIPTIFGVGPHGSFYKDGIYRKSGIQIFIDKAAAGEPIEVYGSTDTT
KDLLYVKDLNIGVLNALKIPTSKGFYNIGYEQNFRLIDIVKAIIEVFSNDNNISTVIQRPDIPNNGSFPLMDITKLKKDI
GFEPHYSNIVDMMEDYKCELERGLYTRLFNVY

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 40433; Mature: 40433

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYFRKLILIKEKKLMNILIIGASSFIGAYQVEQLINTPDFVGGVVGTGRNPRFRDYYES
CCHHHHHHEEECCCEEEEEEEECCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHH
IGVPYYNIDVCDYDSFKVLDQYDFDAAVLFATMMPSNIPKDDDSDDTAEYYKVNVLGTLN
CCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEHHH
ALEYCRTRGINKLISFGTRFDCRLYDSNTVITEETPLNYSLTDDHAAFVMSNNAKWDVMR
HHHHHHHCCHHHHHHCCCCEEEEEECCCEEEECCCCCCEEECCCCEEEEEECCCCCHHHH
YYNEKYEMNNIYFRIPTIFGVGPHGSFYKDGIYRKSGIQIFIDKAAAGEPIEVYGSTDTT
HHCCCEECCEEEEEECEEEEECCCCCHHHCCCEECCCCEEEEECCCCCCCEEEECCCCCC
KDLLYVKDLNIGVLNALKIPTSKGFYNIGYEQNFRLIDIVKAIIEVFSNDNNISTVIQRP
CEEEEEEECCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
DIPNNGSFPLMDITKLKKDIGFEPHYSNIVDMMEDYKCELERGLYTRLFNVY
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYFRKLILIKEKKLMNILIIGASSFIGAYQVEQLINTPDFVGGVVGTGRNPRFRDYYES
CCHHHHHHEEECCCEEEEEEEECCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHH
IGVPYYNIDVCDYDSFKVLDQYDFDAAVLFATMMPSNIPKDDDSDDTAEYYKVNVLGTLN
CCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEHHH
ALEYCRTRGINKLISFGTRFDCRLYDSNTVITEETPLNYSLTDDHAAFVMSNNAKWDVMR
HHHHHHHCCHHHHHHCCCCEEEEEECCCEEEECCCCCCEEECCCCEEEEEECCCCCHHHH
YYNEKYEMNNIYFRIPTIFGVGPHGSFYKDGIYRKSGIQIFIDKAAAGEPIEVYGSTDTT
HHCCCEECCEEEEEECEEEEECCCCCHHHCCCEECCCCEEEEECCCCCCCEEEECCCCCC
KDLLYVKDLNIGVLNALKIPTSKGFYNIGYEQNFRLIDIVKAIIEVFSNDNNISTVIQRP
CEEEEEEECCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
DIPNNGSFPLMDITKLKKDIGFEPHYSNIVDMMEDYKCELERGLYTRLFNVY
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA