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The map label for this gene is metG [H]

Identifier: 238925543

GI number: 238925543

Start: 3044663

End: 3046684

Strand: Reverse

Name: metG [H]

Synonym: EUBREC_3199

Alternate gene names: 238925543

Gene position: 3046684-3044663 (Counterclockwise)

Preceding gene: 238925544

Following gene: 238925542

Centisome position: 88.32

GC content: 46.69

Gene sequence:

>2022_bases
ATGACAACAGCCATTGCGTACACATCAGGCAAGCCTCATATTGGAAACACATATGAGATCATCCTTGCAGACGCAATCGC
CAGATACAAGAGAGCAGAGGGATACGACGTATATTTCCAGACAGGAACAGATGAGCACGGACAGAAAATCCAGGAAAAGG
CTGAGGCAGCCGGAATTTCACCGAAGGAGTATGTAGATCAGATTTCAGGTGAGGTAAAGAGAATCTGGGATCTGGTAAAT
TCATCATATGACAATTTTGTCCGCACAACAGATGAGGACCATGAGAAATGTGTAAAGAAAATCTTCAAGAAGCTTTACGA
TCAGGGCGACATCTACAAGGGCTCATACGAGGGACTTTACTGTACACCATGTGAGTCATTCTGGACAGAGTCACAGCTCG
TGGACGGAAAATGCCCGGACTGCGGACGTGAGGTACAGCCTGCAAAGGAGGAGGCATACTTCTTCAAGATGAGCAAATAT
GCAGACAGACTCATCGACTATATCAACACACACCCTGATTTCATCCAGCCGGTTTCAAGGAAAAACGAGATGATGAACAA
CTTCCTGCTTCCGGGACTTCAGGATCTTTGCGTATCAAGAACATCATTCAGCTGGGGAATTCCGGTAGATTTCGATCCAA
AGCATGTAGTGTATGTATGGCTTGATGCCCTCACAAACTATATTACAAAAATCGGTTACGATCCTGACGGATCATCAGAG
CTTTTCAAGAAAAACTGGCCTGCAGATCTGCACCTTATCGGAAAAGATATCGTGCGTTTCCACACTATTTACTGGCCTAT
TTTCCTTATGGCACTTGACCTTCCGCTTCCAAAGCAGGTATTCGGACATCCATGGCTGCTTCAGGGCGGTGACAAGATGA
GTAAGTCAAAAGGAAACGTTATCTACGCAGACGATATGGTCAGGCTTTTCGGCGTGGATGCCACACGTTACTTTGTGCTT
CATGAGATGCCATTCGAGAACGACGGAATCATCACATGGGATCTTGTAATCGAGAGATTCAACTCAGACCTTGCAAACAT
ACTTGGAAACCTTGTAAACAGAACAGTTTCCATGAGCAATAAGTATTTTGACGGCATCGTAAAGAGCACAGGCGCAACAG
GCGATGCAGATCAGACTGCCATTGACGCAGATCTTAAGGCTGTTGTTACCGGCACAAGAGATAAAGTGGCAAAGAAAATG
GAAGGACTTCGTGTGGCAGATGCCATCACAGAGGTATTCGCACTTTTCCGCCGCTGCAACAAATACATCGACGAGACAAC
ACCTTGGGTGCTTGCAAAGGACGAGGCACAGCAGGATCGTCTTGCTGAGGTGCTTTACAACCTCACAGAGTCTATCACAA
TCGGAGCAAGCCTGCTTCATTCATTCCTGCCTGAGACAGCAGAAAAGATTGTGGCACAGCTTAACACAACACTCCGTGAG
TTCGATGACCTTGACAAATTTGGTCTTTATGAGAGCGGCACAAAGGTTACAGACAAACCTGAGATCCTCTTCGGCAGATT
AAAGGCAGAGGATGTGATGCCTGAGGTAGAGAAAATCCAGGCGGTACAGAAAGCAGAGTTTGAGGCTGAGCAGGCTAAGC
TTGCTGCAGTTGAGGGTAAGGCAGCATGTGGCTGTGGTGCAGGTGAAAATGCCGCAGATGGTGCAGACCTTGAGCCGATG
GATGTGGAAGCAAAGGAAGAAATCACATTTGATGATTTCGAAAAGCTTCAGTTCCAGGTTGGTGAAATCATAAAATGTGA
GGCAGTTGCGAAGTCAAAGAAGCTTCTCTGCTCACAGGTAAAGATTGGAAACCAGACAAAGCAGATAGTATCAGGTATCA
GAAAGTACTATTCTCCTGAGGAGATGGTAGGCAAGAAGGTAATGGTTCTCGTAAACTTAAAACCGGCTAAGCTCGCAGGT
GTTTTATCAGAGGGAATGCTTCTGTGCGCAGAGGATGCAGAGGGCAATCTCGCACTCCTGACACCGGAGAAGCCAATGCC
GGCAGGAGCACCTATTGAGTGA

Upstream 100 bases:

>100_bases
TGAGGAATATACTGTATGTGATACGGCATTATAGAAGACTATATGTGCGAAGCATTAAAAACAGGAGGAAACTAAATTGG
CAAACAAAGGTAAATATTAC

Downstream 100 bases:

>100_bases
TCCGGACGTAAGCGACAAGAAAGGAGTCTCATATGGAATATTTTGATATAGTGGACGAGCAGGGAAATCCAACAGGACAG
ACCGTAGAGCGCAAGCAGGC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MTTAIAYTSGKPHIGNTYEIILADAIARYKRAEGYDVYFQTGTDEHGQKIQEKAEAAGISPKEYVDQISGEVKRIWDLVN
SSYDNFVRTTDEDHEKCVKKIFKKLYDQGDIYKGSYEGLYCTPCESFWTESQLVDGKCPDCGREVQPAKEEAYFFKMSKY
ADRLIDYINTHPDFIQPVSRKNEMMNNFLLPGLQDLCVSRTSFSWGIPVDFDPKHVVYVWLDALTNYITKIGYDPDGSSE
LFKKNWPADLHLIGKDIVRFHTIYWPIFLMALDLPLPKQVFGHPWLLQGGDKMSKSKGNVIYADDMVRLFGVDATRYFVL
HEMPFENDGIITWDLVIERFNSDLANILGNLVNRTVSMSNKYFDGIVKSTGATGDADQTAIDADLKAVVTGTRDKVAKKM
EGLRVADAITEVFALFRRCNKYIDETTPWVLAKDEAQQDRLAEVLYNLTESITIGASLLHSFLPETAEKIVAQLNTTLRE
FDDLDKFGLYESGTKVTDKPEILFGRLKAEDVMPEVEKIQAVQKAEFEAEQAKLAAVEGKAACGCGAGENAADGADLEPM
DVEAKEEITFDDFEKLQFQVGEIIKCEAVAKSKKLLCSQVKIGNQTKQIVSGIRKYYSPEEMVGKKVMVLVNLKPAKLAG
VLSEGMLLCAEDAEGNLALLTPEKPMPAGAPIE

Sequences:

>Translated_673_residues
MTTAIAYTSGKPHIGNTYEIILADAIARYKRAEGYDVYFQTGTDEHGQKIQEKAEAAGISPKEYVDQISGEVKRIWDLVN
SSYDNFVRTTDEDHEKCVKKIFKKLYDQGDIYKGSYEGLYCTPCESFWTESQLVDGKCPDCGREVQPAKEEAYFFKMSKY
ADRLIDYINTHPDFIQPVSRKNEMMNNFLLPGLQDLCVSRTSFSWGIPVDFDPKHVVYVWLDALTNYITKIGYDPDGSSE
LFKKNWPADLHLIGKDIVRFHTIYWPIFLMALDLPLPKQVFGHPWLLQGGDKMSKSKGNVIYADDMVRLFGVDATRYFVL
HEMPFENDGIITWDLVIERFNSDLANILGNLVNRTVSMSNKYFDGIVKSTGATGDADQTAIDADLKAVVTGTRDKVAKKM
EGLRVADAITEVFALFRRCNKYIDETTPWVLAKDEAQQDRLAEVLYNLTESITIGASLLHSFLPETAEKIVAQLNTTLRE
FDDLDKFGLYESGTKVTDKPEILFGRLKAEDVMPEVEKIQAVQKAEFEAEQAKLAAVEGKAACGCGAGENAADGADLEPM
DVEAKEEITFDDFEKLQFQVGEIIKCEAVAKSKKLLCSQVKIGNQTKQIVSGIRKYYSPEEMVGKKVMVLVNLKPAKLAG
VLSEGMLLCAEDAEGNLALLTPEKPMPAGAPIE
>Mature_672_residues
TTAIAYTSGKPHIGNTYEIILADAIARYKRAEGYDVYFQTGTDEHGQKIQEKAEAAGISPKEYVDQISGEVKRIWDLVNS
SYDNFVRTTDEDHEKCVKKIFKKLYDQGDIYKGSYEGLYCTPCESFWTESQLVDGKCPDCGREVQPAKEEAYFFKMSKYA
DRLIDYINTHPDFIQPVSRKNEMMNNFLLPGLQDLCVSRTSFSWGIPVDFDPKHVVYVWLDALTNYITKIGYDPDGSSEL
FKKNWPADLHLIGKDIVRFHTIYWPIFLMALDLPLPKQVFGHPWLLQGGDKMSKSKGNVIYADDMVRLFGVDATRYFVLH
EMPFENDGIITWDLVIERFNSDLANILGNLVNRTVSMSNKYFDGIVKSTGATGDADQTAIDADLKAVVTGTRDKVAKKME
GLRVADAITEVFALFRRCNKYIDETTPWVLAKDEAQQDRLAEVLYNLTESITIGASLLHSFLPETAEKIVAQLNTTLREF
DDLDKFGLYESGTKVTDKPEILFGRLKAEDVMPEVEKIQAVQKAEFEAEQAKLAAVEGKAACGCGAGENAADGADLEPMD
VEAKEEITFDDFEKLQFQVGEIIKCEAVAKSKKLLCSQVKIGNQTKQIVSGIRKYYSPEEMVGKKVMVLVNLKPAKLAGV
LSEGMLLCAEDAEGNLALLTPEKPMPAGAPIE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=545, Percent_Identity=33.2110091743119, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI14043022, Length=516, Percent_Identity=26.937984496124, Blast_Score=179, Evalue=1e-44,
Organism=Escherichia coli, GI1788432, Length=702, Percent_Identity=28.3475783475783, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=44.4444444444444, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1786861, Length=183, Percent_Identity=25.1366120218579, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI212645096, Length=507, Percent_Identity=32.3471400394477, Blast_Score=252, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI71992781, Length=402, Percent_Identity=31.3432835820896, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17540794, Length=582, Percent_Identity=25.9450171821306, Blast_Score=174, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321610, Length=519, Percent_Identity=30.8285163776493, Blast_Score=256, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321704, Length=551, Percent_Identity=23.4119782214156, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21358483, Length=490, Percent_Identity=31.6326530612245, Blast_Score=247, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24655311, Length=551, Percent_Identity=27.7676950998185, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75337; Mature: 75205

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAIAYTSGKPHIGNTYEIILADAIARYKRAEGYDVYFQTGTDEHGQKIQEKAEAAGIS
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCC
PKEYVDQISGEVKRIWDLVNSSYDNFVRTTDEDHEKCVKKIFKKLYDQGDIYKGSYEGLY
HHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
CTPCESFWTESQLVDGKCPDCGREVQPAKEEAYFFKMSKYADRLIDYINTHPDFIQPVSR
ECCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KNEMMNNFLLPGLQDLCVSRTSFSWGIPVDFDPKHVVYVWLDALTNYITKIGYDPDGSSE
HHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCHH
LFKKNWPADLHLIGKDIVRFHTIYWPIFLMALDLPLPKQVFGHPWLLQGGDKMSKSKGNV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEECCCCCCCCCCCCE
IYADDMVRLFGVDATRYFVLHEMPFENDGIITWDLVIERFNSDLANILGNLVNRTVSMSN
EEECCHHHHHCCCHHEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCH
KYFDGIVKSTGATGDADQTAIDADLKAVVTGTRDKVAKKMEGLRVADAITEVFALFRRCN
HHHHHHHHCCCCCCCCCCHHHHCCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KYIDETTPWVLAKDEAQQDRLAEVLYNLTESITIGASLLHSFLPETAEKIVAQLNTTLRE
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDDLDKFGLYESGTKVTDKPEILFGRLKAEDVMPEVEKIQAVQKAEFEAEQAKLAAVEGK
HHCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AACGCGAGENAADGADLEPMDVEAKEEITFDDFEKLQFQVGEIIKCEAVAKSKKLLCSQV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KIGNQTKQIVSGIRKYYSPEEMVGKKVMVLVNLKPAKLAGVLSEGMLLCAEDAEGNLALL
HCCCHHHHHHHHHHHHCCCHHHCCCEEEEEEECCHHHHHHHHHCCCEEEEECCCCCEEEE
TPEKPMPAGAPIE
CCCCCCCCCCCCC
>Mature Secondary Structure 
TTAIAYTSGKPHIGNTYEIILADAIARYKRAEGYDVYFQTGTDEHGQKIQEKAEAAGIS
CEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCC
PKEYVDQISGEVKRIWDLVNSSYDNFVRTTDEDHEKCVKKIFKKLYDQGDIYKGSYEGLY
HHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
CTPCESFWTESQLVDGKCPDCGREVQPAKEEAYFFKMSKYADRLIDYINTHPDFIQPVSR
ECCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KNEMMNNFLLPGLQDLCVSRTSFSWGIPVDFDPKHVVYVWLDALTNYITKIGYDPDGSSE
HHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCHH
LFKKNWPADLHLIGKDIVRFHTIYWPIFLMALDLPLPKQVFGHPWLLQGGDKMSKSKGNV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEECCCCCCCCCCCCE
IYADDMVRLFGVDATRYFVLHEMPFENDGIITWDLVIERFNSDLANILGNLVNRTVSMSN
EEECCHHHHHCCCHHEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCH
KYFDGIVKSTGATGDADQTAIDADLKAVVTGTRDKVAKKMEGLRVADAITEVFALFRRCN
HHHHHHHHCCCCCCCCCCHHHHCCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KYIDETTPWVLAKDEAQQDRLAEVLYNLTESITIGASLLHSFLPETAEKIVAQLNTTLRE
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDDLDKFGLYESGTKVTDKPEILFGRLKAEDVMPEVEKIQAVQKAEFEAEQAKLAAVEGK
HHCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AACGCGAGENAADGADLEPMDVEAKEEITFDDFEKLQFQVGEIIKCEAVAKSKKLLCSQV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KIGNQTKQIVSGIRKYYSPEEMVGKKVMVLVNLKPAKLAGVLSEGMLLCAEDAEGNLALL
HCCCHHHHHHHHHHHHCCCHHHCCCEEEEEEECCHHHHHHHHHCCCEEEEECCCCCEEEE
TPEKPMPAGAPIE
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]