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The map label for this gene is pbpD [H]

Identifier: 238925495

GI number: 238925495

Start: 3001349

End: 3003772

Strand: Reverse

Name: pbpD [H]

Synonym: EUBREC_3151

Alternate gene names: 238925495

Gene position: 3003772-3001349 (Counterclockwise)

Preceding gene: 238925496

Following gene: 238925494

Centisome position: 87.07

GC content: 45.71

Gene sequence:

>2424_bases
ATGGCAAAACAGAAAAAGAAAAAGAAGAAAAAGCAGCATCGCGTTTTCTGGTTTTTCATAAAGCTTCAGATTTTTCTGAT
GGTACTGATACTTGGTGGATTATGCTTTTATTACTTTGGAGGCTATGCAGACAAGGTGGCGAAGCTTCACTCAGAGGCAG
TAGAGCTCGTGCAGAAATCTGACAAAAACACATTTTTACCGGCGCGCACGAGTACACTCTACGATACAAACGGTGATGAG
ATATCAGAGACGGCCACTACCAAAAAGGCTGACTATGTGAAGTATGAGGATATACCGCAGAACTTCGTAAACTGCATGGT
CAGTATCGAGGACAAGAAGTTCTACAAGCACAATGGTGTGGACCTAAAGGCAATAGTGCGTGCCGCAAAGTCCATCATAA
AGAACAAGAGAATTACCCAGGGTGGCAGTACCATCACGATGCAGCTCGCCCGAAATATTTATCTGGATACGAACAAGAAC
TGGCAGCGAAAAGTCAAGGAAATGTTCATAGCGATGGAGCTTGAAAGGAAATATTCAAAGAACGATATTATGGAGTTCTA
CCTGAATAATGTCTATTTCATGAACGGATATTATGGTATTCAGGCAGCGTGCCATGGCTATTTTAACTGTGAGCTTTCGG
ATCTTGATTTGTCGCAGACTGCCTTTTTGTGTGCGATTCCGAACAGCCCGACATACTATGACCCGATAAACAACAAGGAT
CATACATTGACACGTCGCAATCTCATATTGAAAAATCTAAGGGAAGATGGCAAAATCACACAGCAGGAATATGAGGCCGC
TATAAACGAGGAGATAACCCTCAATATGCCTGAGAAGGATGACACGGTCAAGTACAATTATGTGGATACCTATGCATATT
ACTGCGCTACGAGGGCGCTCATGGAGAATGAGGGCTTCAAGTTCAAGTATTACTTTGACTCAGACGATGAAGAAAAGGAG
TATGATGCTTCATATGATGAGATGTATTCATATTGTCAGAAAAAGCTCTACTCAGACGGTTACAAGATTTATACATCAAT
TGATCTGACTATGCAGCAGCAGCTTCAGGATTCAATTGACCTTACGCTTTTGGACTTTACAGACACGACCGATGATGGCA
CATTTAAGCTGCAGAGTGCTGCAACCTGTATAGACAACGACACAGGCGAGGTGGTTGCAATAGTTGGTGGACGAAGCCAG
GATGCGGTCAGTCATACGCTAAACCGTGCTTTCCAAAGCCATCGTCAGCCGGGAAGCTCAATCAAGCCGCTCATTGTGTA
TACACCATCATTTGAGCGTGGTAAGACACCGGATACCATCGTAAATGACCACAAGTTTGATGGAGGACCGTCTAACTCGG
GTGATTCATATTACGGTGATGTGACTATCAGGTTTGCCGTTCAAAAATCACTGAATACTGTAGCATGGCAGCTATATGAT
GAGCTCACGCCAAAGGTAGGCTTACAGTATCTCAAGGATATGAATTTTACCAATATTGTAAAGGATGACTATGTCACAGC
AACAGCACTTGGAGGTTTTACAACAGGTGTATCCACACTGGAGATGGCATCTGCATATTCAACACTTGAGAACGATGGAA
TGTACAGGAATCCGACATGTATTAAGTCGATTGTAGATTCTGACAACAATATCATATATACATCATCGCAGAAGGATACC
GTCATATATACAGAGACAGCATCACGTATGATGACAGATGTCATGACAGATGTTATGAAATCAGGAACCGGACGTAGCGC
TAATCTGGACAATGGCATGCCTTGCGCCGGCAAAACCGGAACCACAAACGATAAGAAGGATGGCTGGTTCTGTGGCTTTA
CCAGATACTACACCACATGTGTATGGGTAGGCTGCGATATGCCTGAGACAGTCAAAAAACTGACAGGAAGCTCTTACCCG
GCAGAAATATGGAAGAACTACATGGATCAGATACATGCTGACCTTACACCAATAGATTTCCTGCCATATGCCCAAATATC
GGATGATTATCAGGACAGCCAGGAGACACAGGATACACCTGCCGATGACCAGACGCAGAATAATCCTACAGATCAGACAG
TGACCGCACCTGATGCAGGAACCACCACACCGGATAAGACAACAACAGACACGAATAAGACAGATGCTGCTGGTGGAAAT
CATGAAAAAACACCTACTACACCATCAGAAAATCCATCAGGTGGAGCCGGAGACAGTGGTTCAGCCGGAGGCACAGGTGG
AGCTGGAGACAGTGGTTCAGCCGGAGACACAGGTGGAGCCGGAGACAGTGGTTCAACCGGAGGTGCAGGTGGAGCTGGAG
GCAGTGGTTCAACCGGAGGTGCAGGTGGAGCCGGAGGCAGCGGTTCAACCGGAGGCACAGGTGGAGCTACCGGTGGTACC
ACCGGAGGCGAAGCAGCCCAGTAG

Upstream 100 bases:

>100_bases
GTAGTATAATAGACAGTACATATTGGATATAGTTGTCAATTATCTATAAAGTATGCCGATATACAATGGTATACCGATAG
TTTGGAGGTAACGTTTAGAA

Downstream 100 bases:

>100_bases
ATTTTTCTTTGCAGTGCTGTCGGGAGTTGTGTTCTTTGCAGCATACGCATCTGTCACGATAGGAAACAAGACGATAGATG
CGCTCATATATTCAGTGACC

Product: Glycosyltransferase Family 51 candidate bifunctional family GT51 b-glycosyltransferase/PBP transpeptidase (candidate murein polymerase)

Products: NA

Alternate protein names: PBP 4 [H]

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MAKQKKKKKKKQHRVFWFFIKLQIFLMVLILGGLCFYYFGGYADKVAKLHSEAVELVQKSDKNTFLPARTSTLYDTNGDE
ISETATTKKADYVKYEDIPQNFVNCMVSIEDKKFYKHNGVDLKAIVRAAKSIIKNKRITQGGSTITMQLARNIYLDTNKN
WQRKVKEMFIAMELERKYSKNDIMEFYLNNVYFMNGYYGIQAACHGYFNCELSDLDLSQTAFLCAIPNSPTYYDPINNKD
HTLTRRNLILKNLREDGKITQQEYEAAINEEITLNMPEKDDTVKYNYVDTYAYYCATRALMENEGFKFKYYFDSDDEEKE
YDASYDEMYSYCQKKLYSDGYKIYTSIDLTMQQQLQDSIDLTLLDFTDTTDDGTFKLQSAATCIDNDTGEVVAIVGGRSQ
DAVSHTLNRAFQSHRQPGSSIKPLIVYTPSFERGKTPDTIVNDHKFDGGPSNSGDSYYGDVTIRFAVQKSLNTVAWQLYD
ELTPKVGLQYLKDMNFTNIVKDDYVTATALGGFTTGVSTLEMASAYSTLENDGMYRNPTCIKSIVDSDNNIIYTSSQKDT
VIYTETASRMMTDVMTDVMKSGTGRSANLDNGMPCAGKTGTTNDKKDGWFCGFTRYYTTCVWVGCDMPETVKKLTGSSYP
AEIWKNYMDQIHADLTPIDFLPYAQISDDYQDSQETQDTPADDQTQNNPTDQTVTAPDAGTTTPDKTTTDTNKTDAAGGN
HEKTPTTPSENPSGGAGDSGSAGGTGGAGDSGSAGDTGGAGDSGSTGGAGGAGGSGSTGGAGGAGGSGSTGGTGGATGGT
TGGEAAQ

Sequences:

>Translated_807_residues
MAKQKKKKKKKQHRVFWFFIKLQIFLMVLILGGLCFYYFGGYADKVAKLHSEAVELVQKSDKNTFLPARTSTLYDTNGDE
ISETATTKKADYVKYEDIPQNFVNCMVSIEDKKFYKHNGVDLKAIVRAAKSIIKNKRITQGGSTITMQLARNIYLDTNKN
WQRKVKEMFIAMELERKYSKNDIMEFYLNNVYFMNGYYGIQAACHGYFNCELSDLDLSQTAFLCAIPNSPTYYDPINNKD
HTLTRRNLILKNLREDGKITQQEYEAAINEEITLNMPEKDDTVKYNYVDTYAYYCATRALMENEGFKFKYYFDSDDEEKE
YDASYDEMYSYCQKKLYSDGYKIYTSIDLTMQQQLQDSIDLTLLDFTDTTDDGTFKLQSAATCIDNDTGEVVAIVGGRSQ
DAVSHTLNRAFQSHRQPGSSIKPLIVYTPSFERGKTPDTIVNDHKFDGGPSNSGDSYYGDVTIRFAVQKSLNTVAWQLYD
ELTPKVGLQYLKDMNFTNIVKDDYVTATALGGFTTGVSTLEMASAYSTLENDGMYRNPTCIKSIVDSDNNIIYTSSQKDT
VIYTETASRMMTDVMTDVMKSGTGRSANLDNGMPCAGKTGTTNDKKDGWFCGFTRYYTTCVWVGCDMPETVKKLTGSSYP
AEIWKNYMDQIHADLTPIDFLPYAQISDDYQDSQETQDTPADDQTQNNPTDQTVTAPDAGTTTPDKTTTDTNKTDAAGGN
HEKTPTTPSENPSGGAGDSGSAGGTGGAGDSGSAGDTGGAGDSGSTGGAGGAGGSGSTGGAGGAGGSGSTGGTGGATGGT
TGGEAAQ
>Mature_806_residues
AKQKKKKKKKQHRVFWFFIKLQIFLMVLILGGLCFYYFGGYADKVAKLHSEAVELVQKSDKNTFLPARTSTLYDTNGDEI
SETATTKKADYVKYEDIPQNFVNCMVSIEDKKFYKHNGVDLKAIVRAAKSIIKNKRITQGGSTITMQLARNIYLDTNKNW
QRKVKEMFIAMELERKYSKNDIMEFYLNNVYFMNGYYGIQAACHGYFNCELSDLDLSQTAFLCAIPNSPTYYDPINNKDH
TLTRRNLILKNLREDGKITQQEYEAAINEEITLNMPEKDDTVKYNYVDTYAYYCATRALMENEGFKFKYYFDSDDEEKEY
DASYDEMYSYCQKKLYSDGYKIYTSIDLTMQQQLQDSIDLTLLDFTDTTDDGTFKLQSAATCIDNDTGEVVAIVGGRSQD
AVSHTLNRAFQSHRQPGSSIKPLIVYTPSFERGKTPDTIVNDHKFDGGPSNSGDSYYGDVTIRFAVQKSLNTVAWQLYDE
LTPKVGLQYLKDMNFTNIVKDDYVTATALGGFTTGVSTLEMASAYSTLENDGMYRNPTCIKSIVDSDNNIIYTSSQKDTV
IYTETASRMMTDVMTDVMKSGTGRSANLDNGMPCAGKTGTTNDKKDGWFCGFTRYYTTCVWVGCDMPETVKKLTGSSYPA
EIWKNYMDQIHADLTPIDFLPYAQISDDYQDSQETQDTPADDQTQNNPTDQTVTAPDAGTTTPDKTTTDTNKTDAAGGNH
EKTPTTPSENPSGGAGDSGSAGGTGGAGDSGSAGDTGGAGDSGSTGGAGGAGGSGSTGGAGGAGGSGSTGGTGGATGGTT
GGEAAQ

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=350, Percent_Identity=27.7142857142857, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1786343, Length=599, Percent_Identity=26.2103505843072, Blast_Score=161, Evalue=2e-40,
Organism=Escherichia coli, GI1788867, Length=550, Percent_Identity=22.9090909090909, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789601, Length=168, Percent_Identity=29.7619047619048, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 88508; Mature: 88376

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKKKKKKKQHRVFWFFIKLQIFLMVLILGGLCFYYFGGYADKVAKLHSEAVELVQKS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
DKNTFLPARTSTLYDTNGDEISETATTKKADYVKYEDIPQNFVNCMVSIEDKKFYKHNGV
CCCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHEEEEECCCHHHHCCCC
DLKAIVRAAKSIIKNKRITQGGSTITMQLARNIYLDTNKNWQRKVKEMFIAMELERKYSK
CHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHCCH
NDIMEFYLNNVYFMNGYYGIQAACHGYFNCELSDLDLSQTAFLCAIPNSPTYYDPINNKD
HHHHHHHHCCEEEECCCEEHHHHHCCEEECEECCCCCCCCEEEEECCCCCCEECCCCCCC
HTLTRRNLILKNLREDGKITQQEYEAAINEEITLNMPEKDDTVKYNYVDTYAYYCATRAL
CHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCEEEEEHHHHHHHHHHHHH
MENEGFKFKYYFDSDDEEKEYDASYDEMYSYCQKKLYSDGYKIYTSIDLTMQQQLQDSID
HHCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHCCC
LTLLDFTDTTDDGTFKLQSAATCIDNDTGEVVAIVGGRSQDAVSHTLNRAFQSHRQPGSS
EEEEEECCCCCCCCEEECCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCC
IKPLIVYTPSFERGKTPDTIVNDHKFDGGPSNSGDSYYGDVTIRFAVQKSLNTVAWQLYD
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEEEEEHHHHHHHHHHHHH
ELTPKVGLQYLKDMNFTNIVKDDYVTATALGGFTTGVSTLEMASAYSTLENDGMYRNPTC
HHCHHHHHHHHHCCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IKSIVDSDNNIIYTSSQKDTVIYTETASRMMTDVMTDVMKSGTGRSANLDNGMPCAGKTG
HHHHHCCCCCEEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
TTNDKKDGWFCGFTRYYTTCVWVGCDMPETVKKLTGSSYPAEIWKNYMDQIHADLTPIDF
CCCCCCCCEEEEHHHHEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
LPYAQISDDYQDSQETQDTPADDQTQNNPTDQTVTAPDAGTTTPDKTTTDTNKTDAAGGN
CCCCCCCCCHHCCHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCC
HEKTPTTPSENPSGGAGDSGSAGGTGGAGDSGSAGDTGGAGDSGSTGGAGGAGGSGSTGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGGAGGSGSTGGTGGATGGTTGGEAAQ
CCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKQKKKKKKKQHRVFWFFIKLQIFLMVLILGGLCFYYFGGYADKVAKLHSEAVELVQKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
DKNTFLPARTSTLYDTNGDEISETATTKKADYVKYEDIPQNFVNCMVSIEDKKFYKHNGV
CCCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHEEEEECCCHHHHCCCC
DLKAIVRAAKSIIKNKRITQGGSTITMQLARNIYLDTNKNWQRKVKEMFIAMELERKYSK
CHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHCCH
NDIMEFYLNNVYFMNGYYGIQAACHGYFNCELSDLDLSQTAFLCAIPNSPTYYDPINNKD
HHHHHHHHCCEEEECCCEEHHHHHCCEEECEECCCCCCCCEEEEECCCCCCEECCCCCCC
HTLTRRNLILKNLREDGKITQQEYEAAINEEITLNMPEKDDTVKYNYVDTYAYYCATRAL
CHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCEEEEEHHHHHHHHHHHHH
MENEGFKFKYYFDSDDEEKEYDASYDEMYSYCQKKLYSDGYKIYTSIDLTMQQQLQDSID
HHCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHCCC
LTLLDFTDTTDDGTFKLQSAATCIDNDTGEVVAIVGGRSQDAVSHTLNRAFQSHRQPGSS
EEEEEECCCCCCCCEEECCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCC
IKPLIVYTPSFERGKTPDTIVNDHKFDGGPSNSGDSYYGDVTIRFAVQKSLNTVAWQLYD
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEEEEEHHHHHHHHHHHHH
ELTPKVGLQYLKDMNFTNIVKDDYVTATALGGFTTGVSTLEMASAYSTLENDGMYRNPTC
HHCHHHHHHHHHCCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IKSIVDSDNNIIYTSSQKDTVIYTETASRMMTDVMTDVMKSGTGRSANLDNGMPCAGKTG
HHHHHCCCCCEEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
TTNDKKDGWFCGFTRYYTTCVWVGCDMPETVKKLTGSSYPAEIWKNYMDQIHADLTPIDF
CCCCCCCCEEEEHHHHEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
LPYAQISDDYQDSQETQDTPADDQTQNNPTDQTVTAPDAGTTTPDKTTTDTNKTDAAGGN
CCCCCCCCCHHCCHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCC
HEKTPTTPSENPSGGAGDSGSAGGTGGAGDSGSAGDTGGAGDSGSTGGAGGAGGSGSTGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGGAGGSGSTGGTGGATGGTTGGEAAQ
CCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961491; 9274030; 9384377 [H]