Click here to switch to the map view.

The map label for this gene is nagP [H]

Identifier: 238925270

GI number: 238925270

Start: 2806006

End: 2807523

Strand: Reverse

Name: nagP [H]

Synonym: EUBREC_2924

Alternate gene names: 238925270

Gene position: 2807523-2806006 (Counterclockwise)

Preceding gene: 238925271

Following gene: 238925269

Centisome position: 81.38

GC content: 42.95

Gene sequence:

>1518_bases
TTGGAGTATCTCCAGTTTAGTATACATAATGTAAAGGAGGGGAAATTTATGAAATTTCTTCAAAAACTGGGTAAAGCTTT
AATGCTTCCCGTAGCAGTACTTCCTATCTGTGGTATCCTCATGGGTATTGGATATTTTTTATGTCCTGCAACCATGCAGG
GTGGTGACATCGAGGGAGTGCGTAATTTAATAGGTCTGTTTTTAGTTAAAGCAGGGGGCGCTTTGATTGACAACATGGCC
ATCCTTTTTGTAATCGGCGTTGGTGTCGGTATGTCGGAAAAGAACGACGGTACCGGCGGTATTGCAGCTTTAGCTTCATG
GCTGATGATGACAACACTCCTGTCTACAGGCTTTGTTACCACAATCATGCCATCTATCGCTGACAGCGCTACAAAGACAC
TTGCATTCGACAAGATTGAAAACCCATTTATCGGTATCTTAGCCGGTATTATTGGTGCTTTGTGTTACAACAGATTTAAG
GATACAAAGCTTCCTGACTGGCTGTCATTCTTCAGTGGCAAGCGTTGCGTAGCAATTATCGCAGGTGTTGTTTCAATCCT
TGCATCTGTAGTATTACTGTTTGTATGGCCATTACTTTTCGGTGCTCTTGTATCTATCGGTAAAGCAATCGTTGGTCTTG
ATGTAGTCGGCGCAGGTATCTATGCATTCCTGAACCGTCTGTTAATCCCTACAGGATTACACCACGCATTAAACAATGTA
TTCTGGTTCGATACTATCGGACTTGGAGACCTCAAACATTTCTGGGCAGGCGAGACATCAAAGGATGTTTCATGGAGCCT
CGGAATGTACATGTCAGGTTTCTTCCCATGTATGATGTTTGGTATTCCTGGTGCTGCACTTGCCATGGTTAAGTGTGCAA
AGACTGCTAAGAAAAAGGCTGCCATCGGTATCCTTGCTTCTGCAGCTATCTGTGCATTCATCTGTGGTGTTACAGAGCCT
TTCGAGTTTGCATTTATGTTCCTTGCTCCTGTTCTCTATGTGATTTATGCTTTACTTTATGGTATTTTCACAATGATTAC
AGTAGCTCTTGGATTTAGAGCAGGCTTCTCATTCTCAGCCGGTGCGATGGATTTATTATTCTCATCTTCACTTCCGGCTG
CAGCAAAGACATGGCTCATCATTCCTCTTGGAATCGCAGCTTTTATCGTATTCTACATTGTATTCTATTTCGCTATCAAG
AAATGGGATCTTAAGACCCCTGGTAGAGAGGACGATGATGTCGAGGCAGAGAAGAAGGCAGTGCTTTCTAACAATGACTA
CACAGCAGTAGCAAAGACTATTCTTGAGGGATGCGGTGGCAAAGAAAATATTGCAAGCATTGACAACTGCATCACAAGAC
TCAGACTTGAGGTTAAGGATATCACTCAGGTTGATGACAAGAAGATTAAATCTGCAGGTGTTGCAGGCGTTATGAAGCCT
GGCAAGAATTCTGTTCAGGTTATTATCGGAACCAAGGTTCAGTTTGTTGCGGATGAGTTTTCTAAGCTTTGCGAGTAG

Upstream 100 bases:

>100_bases
GTGAGATAAAGATGCTCAAAGAGGGGGATGTATCCGCCGGTGAAGAGATTTTAAGTCTCGCGTAGATTTGAATATAATTA
TATAATAATAACGAACTTTA

Downstream 100 bases:

>100_bases
ATTTGGTCTGCAATATAGCAGTGTTGATTCACATTAGGTGATTCACATAATTTCATAATACATGATTAAAAGCCACACCG
GGTGTTATGCCTGGGGTGGC

Product: PTS system, N-acetylglucosamine-specific IIBC component

Products: NA

Alternate protein names: EIICB-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MEYLQFSIHNVKEGKFMKFLQKLGKALMLPVAVLPICGILMGIGYFLCPATMQGGDIEGVRNLIGLFLVKAGGALIDNMA
ILFVIGVGVGMSEKNDGTGGIAALASWLMMTTLLSTGFVTTIMPSIADSATKTLAFDKIENPFIGILAGIIGALCYNRFK
DTKLPDWLSFFSGKRCVAIIAGVVSILASVVLLFVWPLLFGALVSIGKAIVGLDVVGAGIYAFLNRLLIPTGLHHALNNV
FWFDTIGLGDLKHFWAGETSKDVSWSLGMYMSGFFPCMMFGIPGAALAMVKCAKTAKKKAAIGILASAAICAFICGVTEP
FEFAFMFLAPVLYVIYALLYGIFTMITVALGFRAGFSFSAGAMDLLFSSSLPAAAKTWLIIPLGIAAFIVFYIVFYFAIK
KWDLKTPGREDDDVEAEKKAVLSNNDYTAVAKTILEGCGGKENIASIDNCITRLRLEVKDITQVDDKKIKSAGVAGVMKP
GKNSVQVIIGTKVQFVADEFSKLCE

Sequences:

>Translated_505_residues
MEYLQFSIHNVKEGKFMKFLQKLGKALMLPVAVLPICGILMGIGYFLCPATMQGGDIEGVRNLIGLFLVKAGGALIDNMA
ILFVIGVGVGMSEKNDGTGGIAALASWLMMTTLLSTGFVTTIMPSIADSATKTLAFDKIENPFIGILAGIIGALCYNRFK
DTKLPDWLSFFSGKRCVAIIAGVVSILASVVLLFVWPLLFGALVSIGKAIVGLDVVGAGIYAFLNRLLIPTGLHHALNNV
FWFDTIGLGDLKHFWAGETSKDVSWSLGMYMSGFFPCMMFGIPGAALAMVKCAKTAKKKAAIGILASAAICAFICGVTEP
FEFAFMFLAPVLYVIYALLYGIFTMITVALGFRAGFSFSAGAMDLLFSSSLPAAAKTWLIIPLGIAAFIVFYIVFYFAIK
KWDLKTPGREDDDVEAEKKAVLSNNDYTAVAKTILEGCGGKENIASIDNCITRLRLEVKDITQVDDKKIKSAGVAGVMKP
GKNSVQVIIGTKVQFVADEFSKLCE
>Mature_505_residues
MEYLQFSIHNVKEGKFMKFLQKLGKALMLPVAVLPICGILMGIGYFLCPATMQGGDIEGVRNLIGLFLVKAGGALIDNMA
ILFVIGVGVGMSEKNDGTGGIAALASWLMMTTLLSTGFVTTIMPSIADSATKTLAFDKIENPFIGILAGIIGALCYNRFK
DTKLPDWLSFFSGKRCVAIIAGVVSILASVVLLFVWPLLFGALVSIGKAIVGLDVVGAGIYAFLNRLLIPTGLHHALNNV
FWFDTIGLGDLKHFWAGETSKDVSWSLGMYMSGFFPCMMFGIPGAALAMVKCAKTAKKKAAIGILASAAICAFICGVTEP
FEFAFMFLAPVLYVIYALLYGIFTMITVALGFRAGFSFSAGAMDLLFSSSLPAAAKTWLIIPLGIAAFIVFYIVFYFAIK
KWDLKTPGREDDDVEAEKKAVLSNNDYTAVAKTILEGCGGKENIASIDNCITRLRLEVKDITQVDDKKIKSAGVAGVMKP
GKNSVQVIIGTKVQFVADEFSKLCE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=500, Percent_Identity=40.2, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1787343, Length=494, Percent_Identity=37.8542510121458, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1787908, Length=526, Percent_Identity=34.6007604562738, Blast_Score=259, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 54224; Mature: 54224

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure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CCCCEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]