Click here to switch to the map view.

The map label for this gene is ngoBVM [H]

Identifier: 238925032

GI number: 238925032

Start: 2561449

End: 2562534

Strand: Direct

Name: ngoBVM [H]

Synonym: EUBREC_2683

Alternate gene names: 238925032

Gene position: 2561449-2562534 (Clockwise)

Preceding gene: 238925031

Following gene: 238925033

Centisome position: 74.25

GC content: 56.91

Gene sequence:

>1086_bases
ATGCCGGACATTAAGCTGGGAAGCCTTTTCGACGGGATCGGCGTGTTCCCTCTGGCTGCTTCCCGGTGCGGTATCCGTCC
GGTATGGGCCAGTGAGATTGAAAAAGCGCCCATCTCCATAACCAAAAGGCACTTTCCCGACATGGTGCATTTGGGGGATA
TTACGAAGGTGGACGGCGGGAAAATCCCACCTGTCCATATAATTACCTTCGGTTCCCCCTGTCAGAACCTTTCTCTGATC
GGCAACCGCTCCGGCCTTGCCGGGGCAAAATCAAGCCTGTTCTATCAGGCGTTTCGTATCATACAGGAAATGAGGGATGC
TACTGATAACCTATATCCAGCTATCGCTGTTTGGGAAAACGTCATGGGAGCGTTTTCTACAAATGACCGGATGGACTTTA
GAGCCGTCCTATCCGCCTTCTCGGACACCGAAGTTCCAATGCCTCCTTCGGGAAGATGGGGAAACGCCGGAATGGTGCGA
GGGGGAACGCCTGATGTGTGCTGGCGGCTCATGGACGCCCAGTATTGGGCAGGCTCCCGAAGGCTGGCACGAAGGCAGCG
GATTTTCGTCGTGGCGGATTTTGGAGGCAGACGTGCCGCAGACATACTATTTAAGCCCCGTCCAATGCTCCCACTTCCTC
CGCCTTGCGGAGAGGGCGGGCGGGCCGCCGCCGAAGGAGATCGAACAGCTTCTTTTGAAACAGGGCGGCAGATACCAGTC
ATCCACCCCTTTCAGTGCTTCCGTATGCGGGGAGCGGCAAAAAGGCAGGAAGAAACGGCCTTCCGAAACAGCTTCGGATT
ACCAACTGACCCTTTTCCCACTCTTTTAGCCAGTGATGTAACGCCCTTTGCCTTCTGGTATGAGGGCGACCCGGAGGGCG
GCTGTATCCGTTTTCTGACGGAAACGGAAAGCGAACGGCTGATGGGGCTGCCGGAGGGCTGGACAAAGTACGGGGCGGAC
GGCGTGGAGATCCGGCCTCTGCAACGCTACAAGGCGCTGGGAAACGCGATTGCCCTCCCTTGCGCCGATTACATTATGGC
CGGGATTTATGAGGTGCTGGCTGACCGGGCCGGAAAGGAGGAATGA

Upstream 100 bases:

>100_bases
GAGGATGTAAAAAACGATATTGCGGTTGCCGGGCGGCTGGGCATTGCGGCGACCACACTGGAAGGGGTGCTTGCCGTGAA
GGGACACGGCACCCCGGGCC

Downstream 100 bases:

>100_bases
GCCCATGTTTGAAGCCTATATAACCAATACAGCCCTGTACCCCTTGATGGGGATCGAGGTAGGGACAACGGTACATTTCC
CCATGACGACACAGGAGTTG

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.NgoBV; Cytosine-specific methyltransferase NgoBV; M.NgoV [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MPDIKLGSLFDGIGVFPLAASRCGIRPVWASEIEKAPISITKRHFPDMVHLGDITKVDGGKIPPVHIITFGSPCQNLSLI
GNRSGLAGAKSSLFYQAFRIIQEMRDATDNLYPAIAVWENVMGAFSTNDRMDFRAVLSAFSDTEVPMPPSGRWGNAGMVR
GGTPDVCWRLMDAQYWAGSRRLARRQRIFVVADFGGRRAADILFKPRPMLPLPPPCGEGGRAAAEGDRTASFETGRQIPV
IHPFQCFRMRGAAKRQEETAFRNSFGLPTDPFPTLLASDVTPFAFWYEGDPEGGCIRFLTETESERLMGLPEGWTKYGAD
GVEIRPLQRYKALGNAIALPCADYIMAGIYEVLADRAGKEE

Sequences:

>Translated_361_residues
MPDIKLGSLFDGIGVFPLAASRCGIRPVWASEIEKAPISITKRHFPDMVHLGDITKVDGGKIPPVHIITFGSPCQNLSLI
GNRSGLAGAKSSLFYQAFRIIQEMRDATDNLYPAIAVWENVMGAFSTNDRMDFRAVLSAFSDTEVPMPPSGRWGNAGMVR
GGTPDVCWRLMDAQYWAGSRRLARRQRIFVVADFGGRRAADILFKPRPMLPLPPPCGEGGRAAAEGDRTASFETGRQIPV
IHPFQCFRMRGAAKRQEETAFRNSFGLPTDPFPTLLASDVTPFAFWYEGDPEGGCIRFLTETESERLMGLPEGWTKYGAD
GVEIRPLQRYKALGNAIALPCADYIMAGIYEVLADRAGKEE
>Mature_360_residues
PDIKLGSLFDGIGVFPLAASRCGIRPVWASEIEKAPISITKRHFPDMVHLGDITKVDGGKIPPVHIITFGSPCQNLSLIG
NRSGLAGAKSSLFYQAFRIIQEMRDATDNLYPAIAVWENVMGAFSTNDRMDFRAVLSAFSDTEVPMPPSGRWGNAGMVRG
GTPDVCWRLMDAQYWAGSRRLARRQRIFVVADFGGRRAADILFKPRPMLPLPPPCGEGGRAAAEGDRTASFETGRQIPVI
HPFQCFRMRGAAKRQEETAFRNSFGLPTDPFPTLLASDVTPFAFWYEGDPEGGCIRFLTETESERLMGLPEGWTKYGADG
VEIRPLQRYKALGNAIALPCADYIMAGIYEVLADRAGKEE

Specific function: This methylase recognizes the double-stranded sequence GGNNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBV endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 39739; Mature: 39608

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDIKLGSLFDGIGVFPLAASRCGIRPVWASEIEKAPISITKRHFPDMVHLGDITKVDGG
CCCCCCCHHHCCCCHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHCCCEEECCCEEECCCC
KIPPVHIITFGSPCQNLSLIGNRSGLAGAKSSLFYQAFRIIQEMRDATDNLYPAIAVWEN
CCCCEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VMGAFSTNDRMDFRAVLSAFSDTEVPMPPSGRWGNAGMVRGGTPDVCWRLMDAQYWAGSR
HHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHH
RLARRQRIFVVADFGGRRAADILFKPRPMLPLPPPCGEGGRAAAEGDRTASFETGRQIPV
HHHCCCEEEEEECCCCCEEEHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
IHPFQCFRMRGAAKRQEETAFRNSFGLPTDPFPTLLASDVTPFAFWYEGDPEGGCIRFLT
ECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCEEEEEE
ETESERLMGLPEGWTKYGADGVEIRPLQRYKALGNAIALPCADYIMAGIYEVLADRAGKE
CCCHHHHCCCCCCHHHHCCCCCEECHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCCC
E
C
>Mature Secondary Structure 
PDIKLGSLFDGIGVFPLAASRCGIRPVWASEIEKAPISITKRHFPDMVHLGDITKVDGG
CCCCCCHHHCCCCHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHCCCEEECCCEEECCCC
KIPPVHIITFGSPCQNLSLIGNRSGLAGAKSSLFYQAFRIIQEMRDATDNLYPAIAVWEN
CCCCEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VMGAFSTNDRMDFRAVLSAFSDTEVPMPPSGRWGNAGMVRGGTPDVCWRLMDAQYWAGSR
HHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHH
RLARRQRIFVVADFGGRRAADILFKPRPMLPLPPPCGEGGRAAAEGDRTASFETGRQIPV
HHHCCCEEEEEECCCCCEEEHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
IHPFQCFRMRGAAKRQEETAFRNSFGLPTDPFPTLLASDVTPFAFWYEGDPEGGCIRFLT
ECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCEEEEEE
ETESERLMGLPEGWTKYGADGVEIRPLQRYKALGNAIALPCADYIMAGIYEVLADRAGKE
CCCHHHHCCCCCCHHHHCCCCCEECHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]