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The map label for this gene is ybaZ [C]

Identifier: 238924789

GI number: 238924789

Start: 2310335

End: 2311006

Strand: Direct

Name: ybaZ [C]

Synonym: EUBREC_2440

Alternate gene names: 238924789

Gene position: 2310335-2311006 (Clockwise)

Preceding gene: 238924788

Following gene: 238924797

Centisome position: 66.97

GC content: 46.28

Gene sequence:

>672_bases
ATGAAAACACGACAAGAAGCATTAGACTACGGCTTATCATTTCCAAATACATATCAGGAAGCACCATTCCACGACCCAAA
CTGGCAGCTCATCCGCGTAAAGGACAGCAAAAAGGTTTTCCTGTGGACCTACGAGCGCAATGGTTTTATCAACTTAAATG
TCAAGGTATCTCCTGCCTGGCGCGACTTCTGGAGAGATGCCTTCCCATCGGTCATCCCCGGCTGGCACCAGAACAAGGAC
AACTGGAATACCATCATTCTCGATGGCAGCATACCTGATGACGCTATAAAAAACATGATTTCAGACAGCTATGACCTTGT
CACCTATAATCCGACCCGGCTCATATATGAGGCTGTAAAAAGGATTCCAAAGGGTTGTGTTGCCACATATGCACAGGTTG
CTGAGCTTGCAGGCAATAAAAAAATGTGCCGGGCTGTCGGCAATGCCCTGCACAAGAATCCCAATCCTGATGAAATTCCA
TGCTACAGAGTGGTAAATGCAAAGGGTGAGCTGTCAGGGGCATTTGCCTTCGGCGGAGCCGATGAACAGGCTAACCGTCT
AAGAGCAGATGGCATTGAGGTCATAGACGGCAGGGTTGACCTTGATAAATATGGAATACGGATTGTAGATGATGTTATAT
ATACAAAAACAACAGTGGAACCACTTACTTAA

Upstream 100 bases:

>100_bases
ATGGAGAGATAGAGGGGTTGTATGAAGAATTAAGTCAATAAATTTGTTCTTTTTATGTTATACTGTAATCGTATACCTAA
AAAGAAAGCAGGCAAAATCT

Downstream 100 bases:

>100_bases
ATAATGGTTCCACTGTTATTTTGACAGTTTAGTGTTGTACAGGTGTAAGCTTACCTTTCTCCATTTCAAAAACTTCATCA
CATAACACTTCTATATCTTC

Product: endonuclease V related protein

Products: NA

Alternate protein names: Probable methylated-DNA--protein-cysteine methyltransferase; O-6-methylbase-DNA-alkyltransferase; Endonuclease V; Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKTRQEALDYGLSFPNTYQEAPFHDPNWQLIRVKDSKKVFLWTYERNGFINLNVKVSPAWRDFWRDAFPSVIPGWHQNKD
NWNTIILDGSIPDDAIKNMISDSYDLVTYNPTRLIYEAVKRIPKGCVATYAQVAELAGNKKMCRAVGNALHKNPNPDEIP
CYRVVNAKGELSGAFAFGGADEQANRLRADGIEVIDGRVDLDKYGIRIVDDVIYTKTTVEPLT

Sequences:

>Translated_223_residues
MKTRQEALDYGLSFPNTYQEAPFHDPNWQLIRVKDSKKVFLWTYERNGFINLNVKVSPAWRDFWRDAFPSVIPGWHQNKD
NWNTIILDGSIPDDAIKNMISDSYDLVTYNPTRLIYEAVKRIPKGCVATYAQVAELAGNKKMCRAVGNALHKNPNPDEIP
CYRVVNAKGELSGAFAFGGADEQANRLRADGIEVIDGRVDLDKYGIRIVDDVIYTKTTVEPLT
>Mature_223_residues
MKTRQEALDYGLSFPNTYQEAPFHDPNWQLIRVKDSKKVFLWTYERNGFINLNVKVSPAWRDFWRDAFPSVIPGWHQNKD
NWNTIILDGSIPDDAIKNMISDSYDLVTYNPTRLIYEAVKRIPKGCVATYAQVAELAGNKKMCRAVGNALHKNPNPDEIP
CYRVVNAKGELSGAFAFGGADEQANRLRADGIEVIDGRVDLDKYGIRIVDDVIYTKTTVEPLT

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the endonuclease V family [H]

Homologues:

Organism=Escherichia coli, GI1786659, Length=120, Percent_Identity=35.8333333333333, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 25314; Mature: 25314

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRQEALDYGLSFPNTYQEAPFHDPNWQLIRVKDSKKVFLWTYERNGFINLNVKVSPAW
CCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCEEEEEEEECHHH
RDFWRDAFPSVIPGWHQNKDNWNTIILDGSIPDDAIKNMISDSYDLVTYNPTRLIYEAVK
HHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEECHHHHHHHHHH
RIPKGCVATYAQVAELAGNKKMCRAVGNALHKNPNPDEIPCYRVVNAKGELSGAFAFGGA
HCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCC
DEQANRLRADGIEVIDGRVDLDKYGIRIVDDVIYTKTTVEPLT
CHHHHHHHCCCEEEECCEECHHHCCCEEEHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTRQEALDYGLSFPNTYQEAPFHDPNWQLIRVKDSKKVFLWTYERNGFINLNVKVSPAW
CCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCEEEEEEEECHHH
RDFWRDAFPSVIPGWHQNKDNWNTIILDGSIPDDAIKNMISDSYDLVTYNPTRLIYEAVK
HHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEECHHHHHHHHHH
RIPKGCVATYAQVAELAGNKKMCRAVGNALHKNPNPDEIPCYRVVNAKGELSGAFAFGGA
HCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCC
DEQANRLRADGIEVIDGRVDLDKYGIRIVDDVIYTKTTVEPLT
CHHHHHHHCCCEEEECCEECHHHCCCEEEHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA