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The map label for this gene is cynR [H]

Identifier: 238924356

GI number: 238924356

Start: 1884819

End: 1885724

Strand: Direct

Name: cynR [H]

Synonym: EUBREC_1997

Alternate gene names: 238924356

Gene position: 1884819-1885724 (Clockwise)

Preceding gene: 238924353

Following gene: 238924357

Centisome position: 54.64

GC content: 43.05

Gene sequence:

>906_bases
ATGAACCAGAATCTCTCATCTTACTGGATTTTCTATACTGTCGCCAATGCCGGCAATATATCAAAGGCAGCCAAGGAGCT
CTATATCAGCCAGCCTGCCATCAGCAAGTCAATTCAAAAGCTCGAGGACGGACTGGAATGCAAGCTTTTTTCCAGAAGCT
CCAGAGGTGTCGTCTTGACCGATGAGGGCAGGCTTCTGTATGATCATGTAAGCGAGGCCTTTGAGGCATTAAATGAGGGT
GAGGAAAAGCTGAAGCGCTCAATAGAGCTCGGTGTCGGCCAGCTTAAAATTGGTGTCAGCTCAACCCTCTGCAAATATAT
GCTTTTGCCATATCTCAAGGAATTCATACACAGAAATCCTCATATAAGCATCTCAATCGAATGTCAGTCCACCAATGAGA
CCTTAAAGCTCCTCGATGATGACAAAATAGATATAGGTCTGATTGGAAAACCAGCATCCCTTAAGACTTTCAATTTCTAT
TATCTGGACAACATTGAGGATATATTTGTCACTACAAAGGACTATCTGCGCAACTTAAGCGCCCGTGGTGTCAAAAAGAG
CGAGATTTTGCAAAACTCTACTCTCATGCTTCTCGATAAAGGCAATATGACACGTAAATATATTGATGATTATCTGCAGG
AGAACCAGATACACGTAAAGGACTCTATAGATATATCAAATATGGACCTGCTCATCGACTTTGCCAAAATAGGCGTAGGT
GTGGCCTGCGTCATAAAAAGCATAGTTTCAAAGGAGCTTGCTGACGGTACCCTGATTGAAATTCCTCTCGGCATACCAAT
CCACAAGCGTGAGGTTGGCTTTGCTTACAAGGAGTCTTCAAAGCTTTCCACCTCTCTGCAGACCTTTATCAAGTTTTATG
AGAGCTACACACCAGAGGATTTTTAA

Upstream 100 bases:

>100_bases
AATCTCATGGTATAATAAACACAGATTTGTTGATGTTCACACATTTTTGTGACAAGCAACGATTACAGCCCATTAAAAGT
ACAAACCACAGGAGGTACAA

Downstream 100 bases:

>100_bases
ATAAAAAAATATGACCCCGGAGGTATTATCTTGAAAGAACAAATACACACTATCCCCATATCAGACGCCTTTGACAATGC
CGGCGAATGCCCCTTCTGCT

Product: carbonate dehydratase

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MNQNLSSYWIFYTVANAGNISKAAKELYISQPAISKSIQKLEDGLECKLFSRSSRGVVLTDEGRLLYDHVSEAFEALNEG
EEKLKRSIELGVGQLKIGVSSTLCKYMLLPYLKEFIHRNPHISISIECQSTNETLKLLDDDKIDIGLIGKPASLKTFNFY
YLDNIEDIFVTTKDYLRNLSARGVKKSEILQNSTLMLLDKGNMTRKYIDDYLQENQIHVKDSIDISNMDLLIDFAKIGVG
VACVIKSIVSKELADGTLIEIPLGIPIHKREVGFAYKESSKLSTSLQTFIKFYESYTPEDF

Sequences:

>Translated_301_residues
MNQNLSSYWIFYTVANAGNISKAAKELYISQPAISKSIQKLEDGLECKLFSRSSRGVVLTDEGRLLYDHVSEAFEALNEG
EEKLKRSIELGVGQLKIGVSSTLCKYMLLPYLKEFIHRNPHISISIECQSTNETLKLLDDDKIDIGLIGKPASLKTFNFY
YLDNIEDIFVTTKDYLRNLSARGVKKSEILQNSTLMLLDKGNMTRKYIDDYLQENQIHVKDSIDISNMDLLIDFAKIGVG
VACVIKSIVSKELADGTLIEIPLGIPIHKREVGFAYKESSKLSTSLQTFIKFYESYTPEDF
>Mature_301_residues
MNQNLSSYWIFYTVANAGNISKAAKELYISQPAISKSIQKLEDGLECKLFSRSSRGVVLTDEGRLLYDHVSEAFEALNEG
EEKLKRSIELGVGQLKIGVSSTLCKYMLLPYLKEFIHRNPHISISIECQSTNETLKLLDDDKIDIGLIGKPASLKTFNFY
YLDNIEDIFVTTKDYLRNLSARGVKKSEILQNSTLMLLDKGNMTRKYIDDYLQENQIHVKDSIDISNMDLLIDFAKIGVG
VACVIKSIVSKELADGTLIEIPLGIPIHKREVGFAYKESSKLSTSLQTFIKFYESYTPEDF

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=24.5733788395904, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI157672245, Length=282, Percent_Identity=24.822695035461, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787601, Length=116, Percent_Identity=37.0689655172414, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=23.972602739726, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788748, Length=296, Percent_Identity=22.2972972972973, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082132, Length=292, Percent_Identity=21.9178082191781, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789173, Length=264, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693105, Length=289, Percent_Identity=21.7993079584775, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790399, Length=282, Percent_Identity=23.0496453900709, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789204, Length=303, Percent_Identity=22.4422442244224, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786448, Length=254, Percent_Identity=26.7716535433071, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367136, Length=245, Percent_Identity=25.3061224489796, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788706, Length=267, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789639, Length=117, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081904, Length=300, Percent_Identity=22.6666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787128, Length=147, Percent_Identity=29.2517006802721, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=287, Percent_Identity=21.9512195121951, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788297, Length=239, Percent_Identity=25.1046025104602, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=22.0394736842105, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34025; Mature: 34025

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNLSSYWIFYTVANAGNISKAAKELYISQPAISKSIQKLEDGLECKLFSRSSRGVVLT
CCCCCCEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEE
DEGRLLYDHVSEAFEALNEGEEKLKRSIELGVGQLKIGVSSTLCKYMLLPYLKEFIHRNP
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCC
HISISIECQSTNETLKLLDDDKIDIGLIGKPASLKTFNFYYLDNIEDIFVTTKDYLRNLS
CEEEEEEECCCCCEEEEECCCCEEEEEECCCCCEEEEEEEEECCCCEEEEECHHHHHCHH
ARGVKKSEILQNSTLMLLDKGNMTRKYIDDYLQENQIHVKDSIDISNMDLLIDFAKIGVG
HCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCHHHEEEHHHHCCH
VACVIKSIVSKELADGTLIEIPLGIPIHKREVGFAYKESSKLSTSLQTFIKFYESYTPED
HHHHHHHHHHHHHCCCEEEEEECCCCEECHHCCCEECCCHHHHHHHHHHHHHHHHCCCCC
F
C
>Mature Secondary Structure
MNQNLSSYWIFYTVANAGNISKAAKELYISQPAISKSIQKLEDGLECKLFSRSSRGVVLT
CCCCCCEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEE
DEGRLLYDHVSEAFEALNEGEEKLKRSIELGVGQLKIGVSSTLCKYMLLPYLKEFIHRNP
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCC
HISISIECQSTNETLKLLDDDKIDIGLIGKPASLKTFNFYYLDNIEDIFVTTKDYLRNLS
CEEEEEEECCCCCEEEEECCCCEEEEEECCCCCEEEEEEEEECCCCEEEEECHHHHHCHH
ARGVKKSEILQNSTLMLLDKGNMTRKYIDDYLQENQIHVKDSIDISNMDLLIDFAKIGVG
HCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCHHHEEEHHHHCCH
VACVIKSIVSKELADGTLIEIPLGIPIHKREVGFAYKESSKLSTSLQTFIKFYESYTPED
HHHHHHHHHHHHHCCCEEEEEECCCCEECHHCCCEECCCHHHHHHHHHHHHHHHHCCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]