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The map label for this gene is nfo [H]

Identifier: 238922381

GI number: 238922381

Start: 586397

End: 587263

Strand: Reverse

Name: nfo [H]

Synonym: EUBELI_20617

Alternate gene names: 238922381

Gene position: 587263-586397 (Counterclockwise)

Preceding gene: 238922385

Following gene: 238922380

Centisome position: 93.7

GC content: 39.33

Gene sequence:

>867_bases
ATGTCAAATATAAAGCTTGGAAGTCATGTGGGAATGGCAGGTAAGGAAATGTTTCTTGCATCTGCGAAGGAAGCAGTTTC
TTATGGGGCTAATGTATTTATGTTATACACAGGTGCGCCACAGAATACAAGAAGAAAAGAAATAAGTGAGCTTAATATAG
AAGCTGGCTGGAAGTATGCTAAGGAACATGGAATTGAGGAGATTATTGTACATGCACCTTATATAATAAATCTTGCAAAT
ACAATTAAGCCGGAGACTTATGAGCTTGCTGTTGAATTTCTTGAAAAAGAAATTATAAGGACTGCTGCCATGGGAAGCCA
TATAATGGTGCTTCATCCAGGAAGCCATGTCAATGCCGGAGTTGAAGCCGGAACCGCACAGATTATCAAAGGACTTAATA
CAGTGCTTAACCAGAACAATGATGATGTATATATTGCACTTGAGACAATGGCTGGCAAGGGAAGCGAGATAGGCAGGACA
TTTGAAGAGCTTAAGGCAATCTATGATGGCGTTGACAAGAAGGACAGATTGAGAGTGTGCTTTGATACATGTCATGTTAA
TGATGCTGGTTATGATTTAGTTAATCATTATGATGAAGTATTTGCAGAATTTGACAAAGTGATTGGTCTTGACCAGATAG
CAGTATTTCATATCAATGACAGCATGAATCCGCTTGGAGCGCATAAGGACAGACATGCCAACATTGATAAAGGAAATATA
GGATATGAGACTCTTCACAGACTTGTCCATGATGAAAGATTCTTAGATGTTCCTAAGATTCTTGAGACCCCATGGGTAGC
AGTTGAGGGAACTGATAAGAAAGAGCCTCCTTATAAGGAAGAGATTGCGTGGCTGCTTAAAGATTAA

Upstream 100 bases:

>100_bases
GCATTGACACATAACTATTGCAATATTAAAATTAATTCGTTTGGATTTTCTAACCGGACAGATAATAAATTATACAGGTT
AAATATTGGAGGAATCATAT

Downstream 100 bases:

>100_bases
TAAACTGTAGGAAAGTATGTTGTGAAAGGCGGGATAATAATATGTCAATACCAGCACATGAAGCAAAGTCAAGATTAATA
GAGGGTAATAAAAGATTTAT

Product: deoxyribonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSNIKLGSHVGMAGKEMFLASAKEAVSYGANVFMLYTGAPQNTRRKEISELNIEAGWKYAKEHGIEEIIVHAPYIINLAN
TIKPETYELAVEFLEKEIIRTAAMGSHIMVLHPGSHVNAGVEAGTAQIIKGLNTVLNQNNDDVYIALETMAGKGSEIGRT
FEELKAIYDGVDKKDRLRVCFDTCHVNDAGYDLVNHYDEVFAEFDKVIGLDQIAVFHINDSMNPLGAHKDRHANIDKGNI
GYETLHRLVHDERFLDVPKILETPWVAVEGTDKKEPPYKEEIAWLLKD

Sequences:

>Translated_288_residues
MSNIKLGSHVGMAGKEMFLASAKEAVSYGANVFMLYTGAPQNTRRKEISELNIEAGWKYAKEHGIEEIIVHAPYIINLAN
TIKPETYELAVEFLEKEIIRTAAMGSHIMVLHPGSHVNAGVEAGTAQIIKGLNTVLNQNNDDVYIALETMAGKGSEIGRT
FEELKAIYDGVDKKDRLRVCFDTCHVNDAGYDLVNHYDEVFAEFDKVIGLDQIAVFHINDSMNPLGAHKDRHANIDKGNI
GYETLHRLVHDERFLDVPKILETPWVAVEGTDKKEPPYKEEIAWLLKD
>Mature_287_residues
SNIKLGSHVGMAGKEMFLASAKEAVSYGANVFMLYTGAPQNTRRKEISELNIEAGWKYAKEHGIEEIIVHAPYIINLANT
IKPETYELAVEFLEKEIIRTAAMGSHIMVLHPGSHVNAGVEAGTAQIIKGLNTVLNQNNDDVYIALETMAGKGSEIGRTF
EELKAIYDGVDKKDRLRVCFDTCHVNDAGYDLVNHYDEVFAEFDKVIGLDQIAVFHINDSMNPLGAHKDRHANIDKGNIG
YETLHRLVHDERFLDVPKILETPWVAVEGTDKKEPPYKEEIAWLLKD

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=286, Percent_Identity=32.8671328671329, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531193, Length=268, Percent_Identity=33.955223880597, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322735, Length=275, Percent_Identity=30.1818181818182, Blast_Score=136, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 32205; Mature: 32074

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIKLGSHVGMAGKEMFLASAKEAVSYGANVFMLYTGAPQNTRRKEISELNIEAGWKYA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCHHHCCHHH
KEHGIEEIIVHAPYIINLANTIKPETYELAVEFLEKEIIRTAAMGSHIMVLHPGSHVNAG
HHCCHHHHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCC
VEAGTAQIIKGLNTVLNQNNDDVYIALETMAGKGSEIGRTFEELKAIYDGVDKKDRLRVC
CCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHEEE
FDTCHVNDAGYDLVNHYDEVFAEFDKVIGLDQIAVFHINDSMNPLGAHKDRHANIDKGNI
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC
GYETLHRLVHDERFLDVPKILETPWVAVEGTDKKEPPYKEEIAWLLKD
CHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SNIKLGSHVGMAGKEMFLASAKEAVSYGANVFMLYTGAPQNTRRKEISELNIEAGWKYA
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCHHHCCHHH
KEHGIEEIIVHAPYIINLANTIKPETYELAVEFLEKEIIRTAAMGSHIMVLHPGSHVNAG
HHCCHHHHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCC
VEAGTAQIIKGLNTVLNQNNDDVYIALETMAGKGSEIGRTFEELKAIYDGVDKKDRLRVC
CCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHEEE
FDTCHVNDAGYDLVNHYDEVFAEFDKVIGLDQIAVFHINDSMNPLGAHKDRHANIDKGNI
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC
GYETLHRLVHDERFLDVPKILETPWVAVEGTDKKEPPYKEEIAWLLKD
CHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA