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The map label for this gene is mdeA [H]

Identifier: 238922369

GI number: 238922369

Start: 576643

End: 577959

Strand: Reverse

Name: mdeA [H]

Synonym: EUBELI_20605

Alternate gene names: 238922369

Gene position: 577959-576643 (Counterclockwise)

Preceding gene: 238922373

Following gene: 238922368

Centisome position: 92.22

GC content: 41.08

Gene sequence:

>1317_bases
ATGTCATGTAAACATATAAAGCCGGAGGGAAATATGGATAATTATTCAATTAACACAAAATGCGTCCAGGCAGGCTATAC
TCCTGGAAATGGTGAACCAAGACAGATTCCTATAATCCAGTCTACTACATTTAAGTATGATACAAGTGAGGATATGGGAA
AACTTTTTGACCTTGAAGCAAGTGGATATTTCTACACAAGACTTCAGAATCCAACTAATGATTATGTGGCAGCTAAGATT
GCAGCCCTTGAAGGTGGAAGTGCAGCAATGCTTACTTCATCAGGACAGGCAGCTAATTTCTTCGCTTTATTTAATATTTG
CGAAGCTGGAAGCCATATAGTAGCATCATCTTCTATATATGGAGGAACATTTAACCTTATTGATGTTACTTTGAAGAAGA
TGGGAATTGAAGCAACATTTGTTGACCCTGACTGCACAGAGGAAGAACTTAATGCAGCGTTCAAGGACAACACAAGAGCA
GTATTTGGTGAGACAATTGCCAATCCTGCACTTACAGTATTAGATATTGAGAAGTTTGCAAATGCAGCACATGCACACGG
TGTACCACTTATCGTCGATAATACATTCCCAACACCAGTTAATTGCAGACCAATTGAATGGGGAGCAGATATTGTAACAC
ATTCAACAACTAAATATATGGACGGACATGGAGCAGCAGTCGGTGGAGCTATTGTAGACAGCGGAAATTTTGACTGGATG
GCACATGCAGACAAGTTCCCTGGACTCTGCACACCTGATGAATCATATCATGGAATTACATATGCCGAGAAGTTCGGTAA
AGAAGGTGCGTTTATAACAAAATGTACTTCACAGCTTATGAGAGATTTAGGCTGCATACAGTCTCCACAGAATGCATTTA
TATTAAATCTTGGTCTTGAATCACTTCATGTGCGTATGCCAAGACATGTTGAAAACGGACAGGCTGTTGCAGAATTTCTT
GAGAAGCAGGATAAGGTTGAATTCGTTAATTATCCGGGACTTCCTTCTAACAAGTACTATGAGACAGCTAAGAAGTACAT
GCCAGAAGGTGGCTGCGGAGTAGTTTCATTCGAGTTAAAGGGTGGAAGAGAGGCTGCAGAGAAGTTTATGAAGAATTTAA
AGCTTGCAGCTATCGAAACTCATGTAGCAGATGCGAGAACATGCTGCCTTAACCCTGCAACATCAACACACAGACAGATG
AATGATGAGCAGCTTAAAGCCGCTGGAATTCCAGCAGGACTTATACGTATCTCATGTGGCCTTGAGGATAAGAAGGATCT
GATTGCGGATATTGCACAGGCGCTGGAGGCTATATAA

Upstream 100 bases:

>100_bases
TGATAATAGCATAATTGATATCATAACATATTATGTTAATAAATGTGTAGCCAGATTATTAAAATGTGCTGTTATATGTT
TTGCGTTTGTGATATAATTC

Downstream 100 bases:

>100_bases
TTCTAATAGAGAATTAAAGTTACCAATAAATCTGGATAATTAAGAAATTGAAATGTTTTATATGAATAAAGTCGTTAAAA
TGATGAGATTATTACAAATA

Product: O-acetylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSCKHIKPEGNMDNYSINTKCVQAGYTPGNGEPRQIPIIQSTTFKYDTSEDMGKLFDLEASGYFYTRLQNPTNDYVAAKI
AALEGGSAAMLTSSGQAANFFALFNICEAGSHIVASSSIYGGTFNLIDVTLKKMGIEATFVDPDCTEEELNAAFKDNTRA
VFGETIANPALTVLDIEKFANAAHAHGVPLIVDNTFPTPVNCRPIEWGADIVTHSTTKYMDGHGAAVGGAIVDSGNFDWM
AHADKFPGLCTPDESYHGITYAEKFGKEGAFITKCTSQLMRDLGCIQSPQNAFILNLGLESLHVRMPRHVENGQAVAEFL
EKQDKVEFVNYPGLPSNKYYETAKKYMPEGGCGVVSFELKGGREAAEKFMKNLKLAAIETHVADARTCCLNPATSTHRQM
NDEQLKAAGIPAGLIRISCGLEDKKDLIADIAQALEAI

Sequences:

>Translated_438_residues
MSCKHIKPEGNMDNYSINTKCVQAGYTPGNGEPRQIPIIQSTTFKYDTSEDMGKLFDLEASGYFYTRLQNPTNDYVAAKI
AALEGGSAAMLTSSGQAANFFALFNICEAGSHIVASSSIYGGTFNLIDVTLKKMGIEATFVDPDCTEEELNAAFKDNTRA
VFGETIANPALTVLDIEKFANAAHAHGVPLIVDNTFPTPVNCRPIEWGADIVTHSTTKYMDGHGAAVGGAIVDSGNFDWM
AHADKFPGLCTPDESYHGITYAEKFGKEGAFITKCTSQLMRDLGCIQSPQNAFILNLGLESLHVRMPRHVENGQAVAEFL
EKQDKVEFVNYPGLPSNKYYETAKKYMPEGGCGVVSFELKGGREAAEKFMKNLKLAAIETHVADARTCCLNPATSTHRQM
NDEQLKAAGIPAGLIRISCGLEDKKDLIADIAQALEAI
>Mature_437_residues
SCKHIKPEGNMDNYSINTKCVQAGYTPGNGEPRQIPIIQSTTFKYDTSEDMGKLFDLEASGYFYTRLQNPTNDYVAAKIA
ALEGGSAAMLTSSGQAANFFALFNICEAGSHIVASSSIYGGTFNLIDVTLKKMGIEATFVDPDCTEEELNAAFKDNTRAV
FGETIANPALTVLDIEKFANAAHAHGVPLIVDNTFPTPVNCRPIEWGADIVTHSTTKYMDGHGAAVGGAIVDSGNFDWMA
HADKFPGLCTPDESYHGITYAEKFGKEGAFITKCTSQLMRDLGCIQSPQNAFILNLGLESLHVRMPRHVENGQAVAEFLE
KQDKVEFVNYPGLPSNKYYETAKKYMPEGGCGVVSFELKGGREAAEKFMKNLKLAAIETHVADARTCCLNPATSTHRQMN
DEQLKAAGIPAGLIRISCGLEDKKDLIADIAQALEAI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=34.9881796690307, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=33.096926713948, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=30.6413301662708, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1790375, Length=380, Percent_Identity=32.1052631578947, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1789383, Length=437, Percent_Identity=25.1716247139588, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17538055, Length=424, Percent_Identity=34.4339622641509, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=412, Percent_Identity=30.5825242718447, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=35.377358490566, Blast_Score=197, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=416, Percent_Identity=43.2692307692308, Blast_Score=318, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6319307, Length=407, Percent_Identity=32.6781326781327, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=333, Percent_Identity=27.9279279279279, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6321254, Length=424, Percent_Identity=25.7075471698113, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=417, Percent_Identity=27.0983213429257, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=34.2789598108747, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47471; Mature: 47340

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCKHIKPEGNMDNYSINTKCVQAGYTPGNGEPRQIPIIQSTTFKYDTSEDMGKLFDLEA
CCCCCCCCCCCCCCEEECCEEEEECCCCCCCCCCEEEEEECCEEECCCCHHHHHEEEECC
SGYFYTRLQNPTNDYVAAKIAALEGGSAAMLTSSGQAANFFALFNICEAGSHIVASSSIY
CCEEEEEECCCCCCEEEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCC
GGTFNLIDVTLKKMGIEATFVDPDCTEEELNAAFKDNTRAVFGETIANPALTVLDIEKFA
CCEEHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCEEHHHHHCCCCEEEEEHHHHH
NAAHAHGVPLIVDNTFPTPVNCRPIEWGADIVTHSTTKYMDGHGAAVGGAIVDSGNFDWM
CHHHHCCCEEEECCCCCCCCCCEEECCCCCCEECCCCEEECCCCCEECCEEEECCCCCEE
AHADKFPGLCTPDESYHGITYAEKFGKEGAFITKCTSQLMRDLGCIQSPQNAFILNLGLE
EECCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHH
SLHVRMPRHVENGQAVAEFLEKQDKVEFVNYPGLPSNKYYETAKKYMPEGGCGVVSFELK
HHEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEEC
GGREAAEKFMKNLKLAAIETHVADARTCCLNPATSTHRQMNDEQLKAAGIPAGLIRISCG
CCHHHHHHHHHCCEEEEEHHHHHCCHHEECCCCCHHHHHCCHHHHHHCCCCEEEEEEECC
LEDKKDLIADIAQALEAI
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SCKHIKPEGNMDNYSINTKCVQAGYTPGNGEPRQIPIIQSTTFKYDTSEDMGKLFDLEA
CCCCCCCCCCCCCEEECCEEEEECCCCCCCCCCEEEEEECCEEECCCCHHHHHEEEECC
SGYFYTRLQNPTNDYVAAKIAALEGGSAAMLTSSGQAANFFALFNICEAGSHIVASSSIY
CCEEEEEECCCCCCEEEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCC
GGTFNLIDVTLKKMGIEATFVDPDCTEEELNAAFKDNTRAVFGETIANPALTVLDIEKFA
CCEEHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCEEHHHHHCCCCEEEEEHHHHH
NAAHAHGVPLIVDNTFPTPVNCRPIEWGADIVTHSTTKYMDGHGAAVGGAIVDSGNFDWM
CHHHHCCCEEEECCCCCCCCCCEEECCCCCCEECCCCEEECCCCCEECCEEEECCCCCEE
AHADKFPGLCTPDESYHGITYAEKFGKEGAFITKCTSQLMRDLGCIQSPQNAFILNLGLE
EECCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHH
SLHVRMPRHVENGQAVAEFLEKQDKVEFVNYPGLPSNKYYETAKKYMPEGGCGVVSFELK
HHEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEEC
GGREAAEKFMKNLKLAAIETHVADARTCCLNPATSTHRQMNDEQLKAAGIPAGLIRISCG
CCHHHHHHHHHCCEEEEEHHHHHCCHHEECCCCCHHHHHCCHHHHHHCCCCEEEEEEECC
LEDKKDLIADIAQALEAI
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]