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The map label for this gene is rpoH [H]

Identifier: 238921550

GI number: 238921550

Start: 3589285

End: 3590139

Strand: Reverse

Name: rpoH [H]

Synonym: NT01EI_3702

Alternate gene names: 238921550

Gene position: 3590139-3589285 (Counterclockwise)

Preceding gene: 238921551

Following gene: 238921549

Centisome position: 94.17

GC content: 58.13

Gene sequence:

>855_bases
ATGACCAAAGAAATGCAAACTTTAACCTTGGTTCCCCAGGGCAGTCTGGAGGCCTACGTGCGGGCTGCCAACGCCTATCC
GATGCTGACGGCAGAGGAAGAGCGGGCGCTGGGGGAACGGCTGCATTACAAGGGAGACCTGGATGCAGCCAAACAGCTGA
TCCTGTCGCACCTGCGCTTTGTGGTTCATGTCGCCCGTAACTACGCTGGCTACGGACTGCCGCAGGCAGATCTGATCCAG
GAGGGCAATATCGGCCTGATGAAGGCCGTGCGTCGCTTTAACCCCGAGATGGGGGTGCGCCTGGTATCATTTGCCGTGCA
CTGGATCAAGGCCGAGATCCATGAATATGTGCTGCGCAACTGGCGTATCGTTAAAGTCGCGACTACAAAGGCTCAGCGTA
AGCTGTTCTTTAACCTGCGTAAAACCAAGCAGCGCCTGGGATGGTTTAATCAGGACGAGGTCGAGCTGGTGGCCCGGGAA
CTGGGCGTGAGCGAGAAGGACGTGCTGGAAATGGAGTCGCGCATGGCCGCTCAGGACATGGCGTTCGACCTGCCCGGTGA
TGACGAGCAGGAGGGTGCACCGGCAGCGCCGGTGCTGTTCCTGCAGGATAAGTCCTCTGACTTTGCCAATGAGATCGAGG
ATGACAACTGGGAAAGCCACGCCGCAGATAAGCTGAGCGTCGCCCTGGAGGGGCTGGACGAGCGTAGTCAGCACATCATT
CGCGCCCGTTGGCTGGACAGTGACAATAAGGCCACTCTGCAAGAGTTGGCCGATCGCTATGGCGTTTCCGCAGAGCGCGT
GCGTCAGCTGGAAAAAAATGCCATGAAGAAGCTGCGTATGGCGATAGAAGCCTGA

Upstream 100 bases:

>100_bases
ACGGTCAAATTATTCAGGATCGTGCTAGCCTTTACCGTTTGGACCTTTTGCCCGGCCGAAAGCTGTAACGCACGGACTGT
TATCGTATTGAGAGGGTTTG

Downstream 100 bases:

>100_bases
GCGCGGTAACGGCAGGATAAGGGGCGATGCGACGGCATCGCCTTTTTTGTGTCCGAACGTCGGCATCGCCGCGCCGGGCG
TACGATGGAGGATGTATGGA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTKEMQTLTLVPQGSLEAYVRAANAYPMLTAEEERALGERLHYKGDLDAAKQLILSHLRFVVHVARNYAGYGLPQADLIQ
EGNIGLMKAVRRFNPEMGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVARE
LGVSEKDVLEMESRMAAQDMAFDLPGDDEQEGAPAAPVLFLQDKSSDFANEIEDDNWESHAADKLSVALEGLDERSQHII
RARWLDSDNKATLQELADRYGVSAERVRQLEKNAMKKLRMAIEA

Sequences:

>Translated_284_residues
MTKEMQTLTLVPQGSLEAYVRAANAYPMLTAEEERALGERLHYKGDLDAAKQLILSHLRFVVHVARNYAGYGLPQADLIQ
EGNIGLMKAVRRFNPEMGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVARE
LGVSEKDVLEMESRMAAQDMAFDLPGDDEQEGAPAAPVLFLQDKSSDFANEIEDDNWESHAADKLSVALEGLDERSQHII
RARWLDSDNKATLQELADRYGVSAERVRQLEKNAMKKLRMAIEA
>Mature_283_residues
TKEMQTLTLVPQGSLEAYVRAANAYPMLTAEEERALGERLHYKGDLDAAKQLILSHLRFVVHVARNYAGYGLPQADLIQE
GNIGLMKAVRRFNPEMGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVAREL
GVSEKDVLEMESRMAAQDMAFDLPGDDEQEGAPAAPVLFLQDKSSDFANEIEDDNWESHAADKLSVALEGLDERSQHIIR
ARWLDSDNKATLQELADRYGVSAERVRQLEKNAMKKLRMAIEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=284, Percent_Identity=82.7464788732394, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1789098, Length=281, Percent_Identity=29.1814946619217, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789448, Length=243, Percent_Identity=30.8641975308642, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32394; Mature: 32263

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEMQTLTLVPQGSLEAYVRAANAYPMLTAEEERALGERLHYKGDLDAAKQLILSHLRF
CCCCCCEEEECCCCCHHHHHHHCCCCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVHVARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEMGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVARELGVSEKDVLEMESRMAAQDM
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AFDLPGDDEQEGAPAAPVLFLQDKSSDFANEIEDDNWESHAADKLSVALEGLDERSQHII
EECCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RARWLDSDNKATLQELADRYGVSAERVRQLEKNAMKKLRMAIEA
HHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKEMQTLTLVPQGSLEAYVRAANAYPMLTAEEERALGERLHYKGDLDAAKQLILSHLRF
CCCCCEEEECCCCCHHHHHHHCCCCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVHVARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEMGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVARELGVSEKDVLEMESRMAAQDM
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AFDLPGDDEQEGAPAAPVLFLQDKSSDFANEIEDDNWESHAADKLSVALEGLDERSQHII
EECCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RARWLDSDNKATLQELADRYGVSAERVRQLEKNAMKKLRMAIEA
HHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]