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The map label for this gene is kefB [H]

Identifier: 238921456

GI number: 238921456

Start: 3492212

End: 3494038

Strand: Reverse

Name: kefB [H]

Synonym: NT01EI_3608

Alternate gene names: 238921456

Gene position: 3494038-3492212 (Counterclockwise)

Preceding gene: 238921457

Following gene: 238921455

Centisome position: 91.65

GC content: 61.85

Gene sequence:

>1827_bases
ATGAACGCTGAACCCTCCCTGCTGTCGGCGGTGGTGCTGTTTTTGTTTGCTGCAGTGCTGGCGGTGCCCATCGCCCGCCG
CTTGGGCATCGGCGCCGTGCTCGGCTTTTTGATCGCCGGGATTGCTATCGGCCCCTGGGGTATCGGTCTGATCCGGGATG
TGGAGGAGATCCTGCACTTCTCGGAGATGGGCGTGGTCTTCCTGCTGTTTATCATCGGGTTGGAGCTGAATCCGGGCAAG
CTGTGGCGCCTGCGGCAGCTGATCTTCGGCGCCGGCGCCGCCCAGCTGCTGCTGAGCGCGCTGCTGCTTGGTGGGTTGTT
GGTCGCCAGCGGGTTTGCCTGGCAGGCAGCGGTCATTGGCGGGATTGGTCTGGCCATGTCGTCTACGGCCATGGCGCTGC
AGCTGATGCGCGAAAAGGGGATGAACCGCAATCAGGGTGGCCGCCTCGGGTTTGCGGTGCTGCTGTTCCAGGATATCGCC
GTCGTGCCGGCGCTGGCGCTGATGCCGATCTTGGCCGGGGGAGCCGATCACGCCATCAGCTGGGGGCACATTGCGCTGAA
GCTCGGTGCGCTGGCCGGTATCTGGGTCGGCGGACGGCTGCTGCTGCGCCCGCTGTTTCACTATATCGCCGCCACCGGCG
TGCGTGAAATCTTTACCGCTGCGGCGCTGTTGGTGGTGCTCGGCTCCGCGCTCCTGATGGATATGCTGGGTTTTTCAATG
GCGCTGGGGACCTTTATCGCCGGGGTGCTGTTGGCAGAGAGCGAGTTCCAGCACGAACTTGAGATCGCCATTGAGCCCTT
TAAGGGACTGTTGCTGGGGCTGTTTTTTATTTCGGTCGGTATGGCGCTCAATCTGGGTGTGCTGTATGCCTACTGGGCGC
AGATCCTGGCCGCAGTCGTTGCGCTGGTGATGGTCAAAAGCGCTGTGCTGTATCTGTTGGCGCGATTGTCGCGGATACGC
AGCGCGGTGCGGTTGCAGTTTTCGGCGGTGCTCAGCCAGGGGGGGGAGTTTGCCTTTGTGTTGTTTTCCGCCGCCGCCGC
CCAGCACCTGCTGAGTGCCCAGCAGAGCGCGATGCTGCTGGTGGTGGTGACGCTGTCGATGGTGACCACTCCACTGCTGA
TGCAGGCGCTCGATCGCTTGTTGGCCCAGCGGCTGAACCCGGCGGAGGAGAGTGCAGAAAAACCCTATGTCGCCGATGAT
GAGCCGCAGGTGATCTTGGTTGGCTTTGGCCGTTTTGGCCAGGTTATCGGTCGGCTGCTGATGGCCAATCAGACGCGGGT
CACCGTACTGGAGCGCGATGTCAGCGTGGTCAGCATGATGCGCCGCTACGGTTATAAGGTGTATTACGGCGATGCCACCC
ACCTGGATCTGCTGCATGCGGCTGGAGCGACACGGGCGGAGGCTATCGTCATCACCTGTAATGAGCCGGAGGAGACTATG
GCGGTGGTTCGTTTGTGTCAACAGCACTTTCCACAGCTGCGCATTCTGGCGCGCGCCCGCGGGCGGGTCGAGGCCCACGA
GCTGCTGCAGGCCGGTGTGACGCAGTTTAGCCGGGAGACCTTTTCCAGTGCGCTGGATCTGGGGCGTAAAACCTTGATTG
AAACTGGAGTGCACCCGCATCAGGCCTATCGGGCCCAGCAGCATTTCCGCCGTTTGGACATGCGCATGCTGTACGAGCTG
ATGCCGCATCGGCAAGGGGATATCGCGCAAATTTCCCGCATTCGCGAGGCGCGACGTGAGCTGGAGGAGATCTTTCAGCG
CGAGATGCTGCATGAGCAGTATCAAATGAATAGCTGGGATGAAGAGTGGCCGGAGGATGAGGCGTGA

Upstream 100 bases:

>100_bases
ACGATCTATTGGGCGCGGCGCCAGAAGTTGGAGATGTTGCACGCTCAGGCGCATGACTATGCCGAATGGTTAGCGGCGCC
GCTGCCCACCGGAGATGATG

Downstream 100 bases:

>100_bases
AGAAGCGATTTATTGCCGGAGCGTGCTGCCCCGTCTGTCATGCCATGGATAGTCTGGTGCTGTGGCAGGTTAATGAGCAT
GAGCAGATGCAGTGCGTGCG

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MNAEPSLLSAVVLFLFAAVLAVPIARRLGIGAVLGFLIAGIAIGPWGIGLIRDVEEILHFSEMGVVFLLFIIGLELNPGK
LWRLRQLIFGAGAAQLLLSALLLGGLLVASGFAWQAAVIGGIGLAMSSTAMALQLMREKGMNRNQGGRLGFAVLLFQDIA
VVPALALMPILAGGADHAISWGHIALKLGALAGIWVGGRLLLRPLFHYIAATGVREIFTAAALLVVLGSALLMDMLGFSM
ALGTFIAGVLLAESEFQHELEIAIEPFKGLLLGLFFISVGMALNLGVLYAYWAQILAAVVALVMVKSAVLYLLARLSRIR
SAVRLQFSAVLSQGGEFAFVLFSAAAAQHLLSAQQSAMLLVVVTLSMVTTPLLMQALDRLLAQRLNPAEESAEKPYVADD
EPQVILVGFGRFGQVIGRLLMANQTRVTVLERDVSVVSMMRRYGYKVYYGDATHLDLLHAAGATRAEAIVITCNEPEETM
AVVRLCQQHFPQLRILARARGRVEAHELLQAGVTQFSRETFSSALDLGRKTLIETGVHPHQAYRAQQHFRRLDMRMLYEL
MPHRQGDIAQISRIREARRELEEIFQREMLHEQYQMNSWDEEWPEDEA

Sequences:

>Translated_608_residues
MNAEPSLLSAVVLFLFAAVLAVPIARRLGIGAVLGFLIAGIAIGPWGIGLIRDVEEILHFSEMGVVFLLFIIGLELNPGK
LWRLRQLIFGAGAAQLLLSALLLGGLLVASGFAWQAAVIGGIGLAMSSTAMALQLMREKGMNRNQGGRLGFAVLLFQDIA
VVPALALMPILAGGADHAISWGHIALKLGALAGIWVGGRLLLRPLFHYIAATGVREIFTAAALLVVLGSALLMDMLGFSM
ALGTFIAGVLLAESEFQHELEIAIEPFKGLLLGLFFISVGMALNLGVLYAYWAQILAAVVALVMVKSAVLYLLARLSRIR
SAVRLQFSAVLSQGGEFAFVLFSAAAAQHLLSAQQSAMLLVVVTLSMVTTPLLMQALDRLLAQRLNPAEESAEKPYVADD
EPQVILVGFGRFGQVIGRLLMANQTRVTVLERDVSVVSMMRRYGYKVYYGDATHLDLLHAAGATRAEAIVITCNEPEETM
AVVRLCQQHFPQLRILARARGRVEAHELLQAGVTQFSRETFSSALDLGRKTLIETGVHPHQAYRAQQHFRRLDMRMLYEL
MPHRQGDIAQISRIREARRELEEIFQREMLHEQYQMNSWDEEWPEDEA
>Mature_608_residues
MNAEPSLLSAVVLFLFAAVLAVPIARRLGIGAVLGFLIAGIAIGPWGIGLIRDVEEILHFSEMGVVFLLFIIGLELNPGK
LWRLRQLIFGAGAAQLLLSALLLGGLLVASGFAWQAAVIGGIGLAMSSTAMALQLMREKGMNRNQGGRLGFAVLLFQDIA
VVPALALMPILAGGADHAISWGHIALKLGALAGIWVGGRLLLRPLFHYIAATGVREIFTAAALLVVLGSALLMDMLGFSM
ALGTFIAGVLLAESEFQHELEIAIEPFKGLLLGLFFISVGMALNLGVLYAYWAQILAAVVALVMVKSAVLYLLARLSRIR
SAVRLQFSAVLSQGGEFAFVLFSAAAAQHLLSAQQSAMLLVVVTLSMVTTPLLMQALDRLLAQRLNPAEESAEKPYVADD
EPQVILVGFGRFGQVIGRLLMANQTRVTVLERDVSVVSMMRRYGYKVYYGDATHLDLLHAAGATRAEAIVITCNEPEETM
AVVRLCQQHFPQLRILARARGRVEAHELLQAGVTQFSRETFSSALDLGRKTLIETGVHPHQAYRAQQHFRRLDMRMLYEL
MPHRQGDIAQISRIREARRELEEIFQREMLHEQYQMNSWDEEWPEDEA

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=386, Percent_Identity=26.1658031088083, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1789749, Length=596, Percent_Identity=72.3154362416107, Blast_Score=832, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=592, Percent_Identity=42.9054054054054, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1786685, Length=548, Percent_Identity=27.3722627737226, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 66549; Mature: 66549

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAEPSLLSAVVLFLFAAVLAVPIARRLGIGAVLGFLIAGIAIGPWGIGLIRDVEEILHF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SEMGVVFLLFIIGLELNPGKLWRLRQLIFGAGAAQLLLSALLLGGLLVASGFAWQAAVIG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GIGLAMSSTAMALQLMREKGMNRNQGGRLGFAVLLFQDIAVVPALALMPILAGGADHAIS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
WGHIALKLGALAGIWVGGRLLLRPLFHYIAATGVREIFTAAALLVVLGSALLMDMLGFSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGTFIAGVLLAESEFQHELEIAIEPFKGLLLGLFFISVGMALNLGVLYAYWAQILAAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVMVKSAVLYLLARLSRIRSAVRLQFSAVLSQGGEFAFVLFSAAAAQHLLSAQQSAMLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHH
VVVTLSMVTTPLLMQALDRLLAQRLNPAEESAEKPYVADDEPQVILVGFGRFGQVIGRLL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHH
MANQTRVTVLERDVSVVSMMRRYGYKVYYGDATHLDLLHAAGATRAEAIVITCNEPEETM
HCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECCCHHHH
AVVRLCQQHFPQLRILARARGRVEAHELLQAGVTQFSRETFSSALDLGRKTLIETGVHPH
HHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QAYRAQQHFRRLDMRMLYELMPHRQGDIAQISRIREARRELEEIFQREMLHEQYQMNSWD
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EEWPEDEA
CCCCCCCC
>Mature Secondary Structure
MNAEPSLLSAVVLFLFAAVLAVPIARRLGIGAVLGFLIAGIAIGPWGIGLIRDVEEILHF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SEMGVVFLLFIIGLELNPGKLWRLRQLIFGAGAAQLLLSALLLGGLLVASGFAWQAAVIG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GIGLAMSSTAMALQLMREKGMNRNQGGRLGFAVLLFQDIAVVPALALMPILAGGADHAIS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
WGHIALKLGALAGIWVGGRLLLRPLFHYIAATGVREIFTAAALLVVLGSALLMDMLGFSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGTFIAGVLLAESEFQHELEIAIEPFKGLLLGLFFISVGMALNLGVLYAYWAQILAAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVMVKSAVLYLLARLSRIRSAVRLQFSAVLSQGGEFAFVLFSAAAAQHLLSAQQSAMLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHH
VVVTLSMVTTPLLMQALDRLLAQRLNPAEESAEKPYVADDEPQVILVGFGRFGQVIGRLL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHH
MANQTRVTVLERDVSVVSMMRRYGYKVYYGDATHLDLLHAAGATRAEAIVITCNEPEETM
HCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECCCHHHH
AVVRLCQQHFPQLRILARARGRVEAHELLQAGVTQFSRETFSSALDLGRKTLIETGVHPH
HHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QAYRAQQHFRRLDMRMLYELMPHRQGDIAQISRIREARRELEEIFQREMLHEQYQMNSWD
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EEWPEDEA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA