Click here to switch to the map view.

The map label for this gene is hyaF [H]

Identifier: 238921261

GI number: 238921261

Start: 3290602

End: 3291459

Strand: Reverse

Name: hyaF [H]

Synonym: NT01EI_3404

Alternate gene names: 238921261

Gene position: 3291459-3290602 (Counterclockwise)

Preceding gene: 238921262

Following gene: 238921260

Centisome position: 86.34

GC content: 64.92

Gene sequence:

>858_bases
ATGAGCGAGACCTTTTTCCATCTGTTGGGGCCGGGCACGCAGCCCGATGACGACAGTTTTAGCATGAACCCGCTGCCGAT
CACCTGCGGGGTCAACGGCGATCCGAGCATGGCGGCGCTGGAGCACTGCGCTCACAGTCCGGCGGTGATGGCGCTGCTGA
CGCGGCTGCTGGTTCAGCTGGAACAGGGGATTCCCCCCCTGGGCGAGGTGATCGGCTGGGATCTGTCGGCGCTGGATGAC
GAGGATATGACCTTTCTCAACGCCCTGCTGGGCGAAGGGGAGGTCTCGGTGCGTATTCAGCATCCGGACGGCAGCGAAAG
CGAGATCCAAGAGGCGATCTTCTGTGGCCTGTGGCGGGTTCGCCGTCTGCAGGGGGGCTGCGTGCTGGAGGATCGGCTGG
AGGCGGGCTGTGCGCCGCTGCCGCTGTGGCAGGCGGCCAGCGCCGATACGCTGCCGGATGATTCGCTGCTGCCGCCGCCC
ATCGAGGGGCTGATGAACGGGCTGCCGCTGGCCCATGAGCTGCTGATGCATGTGCGCGATCCGGCGACCCAACCCCACAG
CATCAACCTGACTCAGTTGCCGCTGAGCGAGGCCGATCGTCTGTTTTTAGCGCGCCTGTGCGGGACGGGCTGCATTCAGA
TCCGCACTTTTGGCTACGGTGAGAGCCGTATCGACGCGACGGGGCTGCGCCATGTCTGGCATGTACGCTGTCTGGATACC
CTGAAGGGGACGCTGCTCGACAGCTATGAGGTGTGTACCTTGCCGGAGCTGGTGCTGGCGGCGCCGGAGGATCTGAGCGA
CTCGGCCCAGCGTCTGGGCGATGTCTGTCGTTGGCTGGAAGAGGTGGCCCATGTTTGA

Upstream 100 bases:

>100_bases
CGAACTGCGCGGCGTGCTCAGCGGGATCCACCCGTGGGCCGATCTGATCGCCCTGATGCGCCCGCTGGTTGAATCCGTCC
TTCCGCAGGAGTCCTGATCG

Downstream 100 bases:

>100_bases
GCGCGGCTATGCCCAGGCGATCGCGGATGATGCCCGGCTGGAGTGCAACCTGTGCTGGTGGGTGTACGATCCGGCGCAGG
GGGACAGAGTGGGGCAAATT

Product: hydrogenase-1 operon protein HyaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSETFFHLLGPGTQPDDDSFSMNPLPITCGVNGDPSMAALEHCAHSPAVMALLTRLLVQLEQGIPPLGEVIGWDLSALDD
EDMTFLNALLGEGEVSVRIQHPDGSESEIQEAIFCGLWRVRRLQGGCVLEDRLEAGCAPLPLWQAASADTLPDDSLLPPP
IEGLMNGLPLAHELLMHVRDPATQPHSINLTQLPLSEADRLFLARLCGTGCIQIRTFGYGESRIDATGLRHVWHVRCLDT
LKGTLLDSYEVCTLPELVLAAPEDLSDSAQRLGDVCRWLEEVAHV

Sequences:

>Translated_285_residues
MSETFFHLLGPGTQPDDDSFSMNPLPITCGVNGDPSMAALEHCAHSPAVMALLTRLLVQLEQGIPPLGEVIGWDLSALDD
EDMTFLNALLGEGEVSVRIQHPDGSESEIQEAIFCGLWRVRRLQGGCVLEDRLEAGCAPLPLWQAASADTLPDDSLLPPP
IEGLMNGLPLAHELLMHVRDPATQPHSINLTQLPLSEADRLFLARLCGTGCIQIRTFGYGESRIDATGLRHVWHVRCLDT
LKGTLLDSYEVCTLPELVLAAPEDLSDSAQRLGDVCRWLEEVAHV
>Mature_284_residues
SETFFHLLGPGTQPDDDSFSMNPLPITCGVNGDPSMAALEHCAHSPAVMALLTRLLVQLEQGIPPLGEVIGWDLSALDDE
DMTFLNALLGEGEVSVRIQHPDGSESEIQEAIFCGLWRVRRLQGGCVLEDRLEAGCAPLPLWQAASADTLPDDSLLPPPI
EGLMNGLPLAHELLMHVRDPATQPHSINLTQLPLSEADRLFLARLCGTGCIQIRTFGYGESRIDATGLRHVWHVRCLDTL
KGTLLDSYEVCTLPELVLAAPEDLSDSAQRLGDVCRWLEEVAHV

Specific function: Not known. Could enhance the incorporation of nickel to the hydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupH/hyaF family [H]

Homologues:

Organism=Escherichia coli, GI1787211, Length=283, Percent_Identity=75.9717314487633, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006894 [H]

Pfam domain/function: PF04809 HupH_C [H]

EC number: NA

Molecular weight: Translated: 31054; Mature: 30923

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETFFHLLGPGTQPDDDSFSMNPLPITCGVNGDPSMAALEHCAHSPAVMALLTRLLVQL
CCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
EQGIPPLGEVIGWDLSALDDEDMTFLNALLGEGEVSVRIQHPDGSESEIQEAIFCGLWRV
HHCCCHHHHHHCCCHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RRLQGGCVLEDRLEAGCAPLPLWQAASADTLPDDSLLPPPIEGLMNGLPLAHELLMHVRD
HHHCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
PATQPHSINLTQLPLSEADRLFLARLCGTGCIQIRTFGYGESRIDATGLRHVWHVRCLDT
CCCCCCCCCEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LKGTLLDSYEVCTLPELVLAAPEDLSDSAQRLGDVCRWLEEVAHV
HHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SETFFHLLGPGTQPDDDSFSMNPLPITCGVNGDPSMAALEHCAHSPAVMALLTRLLVQL
CCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
EQGIPPLGEVIGWDLSALDDEDMTFLNALLGEGEVSVRIQHPDGSESEIQEAIFCGLWRV
HHCCCHHHHHHCCCHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RRLQGGCVLEDRLEAGCAPLPLWQAASADTLPDDSLLPPPIEGLMNGLPLAHELLMHVRD
HHHCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
PATQPHSINLTQLPLSEADRLFLARLCGTGCIQIRTFGYGESRIDATGLRHVWHVRCLDT
CCCCCCCCCEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LKGTLLDSYEVCTLPELVLAAPEDLSDSAQRLGDVCRWLEEVAHV
HHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]