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The map label for this gene is lysS

Identifier: 238921195

GI number: 238921195

Start: 3221902

End: 3223419

Strand: Reverse

Name: lysS

Synonym: NT01EI_3337

Alternate gene names: 238921195

Gene position: 3223419-3221902 (Counterclockwise)

Preceding gene: 238921196

Following gene: 238921194

Centisome position: 84.55

GC content: 55.99

Gene sequence:

>1518_bases
ATGTCTGAACAACAGCAACAAGGCGCCGAACAGGCTCTGGACTTAAACAACGAAATGCAGGCTCGCCGCGAGAAACTGGC
GGCTCTGCGTAAGGAAGGCATCGCTTTCCCTAACGATTTCCGCCGCGACACCACCTCTGATAAGCTGCACAGCCTGTATG
ACGGTAAGAGCAAGGAAGAGCTGGAAGCGCTGAACGTTGAAGTTTCCGTTGCCGGGCGCATGATGACCCGCCGTATCATG
GGTAAAGCCTCTTTCGTTACGCTGCAGGATATGGGTGGCCGTATTCAGCTGTATGTTGCACGTGACGATCTGAAAGACGA
TGTTTACAGCGAACAGTTCAAGAAATGGGACCTGGGCGACATCATCGGCGCACGCGGTACTCTGTTCCGTACTCAGACCG
GCGAGCTGTCCATTCATTGCCACTCTATCCATCTGCTGACCAAGGCCCTGCGCCCGCTGCCGGACAAATTCCACGGTCTG
TCTGACCAGGAAACCCGCTATCGCCAGCGCTATCTGGACCTGATCGCCAACGAATCTTCCCGCGAGACTTTCCGTACCCG
CTCCAAGATCCTGGCTGCTATCCGCAACTTCATGGTCAACAAAGGCTTCATGGAAGTCGAAACCCCGATGATGCAGGTGA
TCCCGGGGGGCGCATCCGCCCGTCCGTTCATCACCCACCACAATGCGCTGGACATCGATATGTACCTGCGTATTGCGCCG
GAGCTGTACCTGAAGCGTCTGGTGGTCGGCGGCTTCGAGCGCGTGTTCGAAATCAACCGTAACTTCCGTAACGAAGGCGT
CTCTCCGCGTCATAACCCTGAGTTCACCATGATGGAACTCTATATGGCCTATGCGGACTACAAAGACCTGATCGTCCTGA
CCGAAGAGCTGTTCCGCACCATCAGCCAGGATGTTCTGGGCAGCAGCGTGGTGCAGTATGGCGATCAGACCTTCGACTTC
GGCAAGCCGTTCATCAAGATGAGCATGAAAGAGGCCATCTGCCACTACCGTCCGGACATCGCCTCCGCCGATTTGGATGA
CATGGATAAAGCCTGCAAGATCGCCGAATCCCTGGGTATCAAGATCGAGAAAAGCTGGGGTCTGGGCCGCGTACAGTGCG
AAATCTTTGATGAAACGGCAGAGAGCCAGCTGATTCAGCCGACCTTCATCACCGAATACCCGGCTGAGGTTTCTCCGCTG
GCGCGCCGTAACGACGATAACCCGTTCATCACCGACCGCTTTGAGTTCTTCATCGGCGGCCGTGAAATCGGTAACGGTTT
CTCTGAGCTGAACGACGCCGAAGATCAGGCAGAGCGTTTCCAGGATCAGGTGAAAGCCAAAGATGCCGGTGACGACGAAG
CCATGTTCTATGACGAAGACTACGTGACCGCACTGGAGCACGGTCTGCCGCCGACCGCAGGCCTGGGCGTGGGTATCGAC
CGTATGATGATGTTGTTCACCAACAGCCATACCATCCGTGACGTGATCCTGTTCCCGGCGATGCGTCCGCAGAAATAA

Upstream 100 bases:

>100_bases
ACGTACCAGCGTTGAGACACGTAATACACAGGCCGTGTTAGATGGCGACCTGGATAGGTTTATTGAGGCAAGTTTAAAAG
CCGGGTTATGAGGAAACACT

Downstream 100 bases:

>100_bases
GTGTTGTCTTAATCGCTTATCAGCCCCGGCTATGCCGGGGTTTTTTTTGTGTACAGCCCCCCGGCCAGGCTGTGGGTTCA
GTAACGCTTTCAGCTTTGAG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSEQQQQGAEQALDLNNEMQARREKLAALRKEGIAFPNDFRRDTTSDKLHSLYDGKSKEELEALNVEVSVAGRMMTRRIM
GKASFVTLQDMGGRIQLYVARDDLKDDVYSEQFKKWDLGDIIGARGTLFRTQTGELSIHCHSIHLLTKALRPLPDKFHGL
SDQETRYRQRYLDLIANESSRETFRTRSKILAAIRNFMVNKGFMEVETPMMQVIPGGASARPFITHHNALDIDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTEELFRTISQDVLGSSVVQYGDQTFDF
GKPFIKMSMKEAICHYRPDIASADLDDMDKACKIAESLGIKIEKSWGLGRVQCEIFDETAESQLIQPTFITEYPAEVSPL
ARRNDDNPFITDRFEFFIGGREIGNGFSELNDAEDQAERFQDQVKAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGVGID
RMMMLFTNSHTIRDVILFPAMRPQK

Sequences:

>Translated_505_residues
MSEQQQQGAEQALDLNNEMQARREKLAALRKEGIAFPNDFRRDTTSDKLHSLYDGKSKEELEALNVEVSVAGRMMTRRIM
GKASFVTLQDMGGRIQLYVARDDLKDDVYSEQFKKWDLGDIIGARGTLFRTQTGELSIHCHSIHLLTKALRPLPDKFHGL
SDQETRYRQRYLDLIANESSRETFRTRSKILAAIRNFMVNKGFMEVETPMMQVIPGGASARPFITHHNALDIDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTEELFRTISQDVLGSSVVQYGDQTFDF
GKPFIKMSMKEAICHYRPDIASADLDDMDKACKIAESLGIKIEKSWGLGRVQCEIFDETAESQLIQPTFITEYPAEVSPL
ARRNDDNPFITDRFEFFIGGREIGNGFSELNDAEDQAERFQDQVKAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGVGID
RMMMLFTNSHTIRDVILFPAMRPQK
>Mature_504_residues
SEQQQQGAEQALDLNNEMQARREKLAALRKEGIAFPNDFRRDTTSDKLHSLYDGKSKEELEALNVEVSVAGRMMTRRIMG
KASFVTLQDMGGRIQLYVARDDLKDDVYSEQFKKWDLGDIIGARGTLFRTQTGELSIHCHSIHLLTKALRPLPDKFHGLS
DQETRYRQRYLDLIANESSRETFRTRSKILAAIRNFMVNKGFMEVETPMMQVIPGGASARPFITHHNALDIDMYLRIAPE
LYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTEELFRTISQDVLGSSVVQYGDQTFDFG
KPFIKMSMKEAICHYRPDIASADLDDMDKACKIAESLGIKIEKSWGLGRVQCEIFDETAESQLIQPTFITEYPAEVSPLA
RRNDDNPFITDRFEFFIGGREIGNGFSELNDAEDQAERFQDQVKAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGVGIDR
MMMLFTNSHTIRDVILFPAMRPQK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=514, Percent_Identity=42.0233463035019, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI194272210, Length=514, Percent_Identity=42.0233463035019, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI45439306, Length=464, Percent_Identity=25, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI40789249, Length=268, Percent_Identity=26.4925373134328, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1789256, Length=505, Percent_Identity=80.990099009901, Blast_Score=867, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=505, Percent_Identity=80.990099009901, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI87082379, Length=321, Percent_Identity=29.595015576324, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788173, Length=310, Percent_Identity=25.4838709677419, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=487, Percent_Identity=41.8891170431211, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535925, Length=487, Percent_Identity=41.8891170431211, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71994340, Length=487, Percent_Identity=41.8891170431211, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17551876, Length=503, Percent_Identity=25.4473161033797, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32566633, Length=243, Percent_Identity=27.5720164609054, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=512, Percent_Identity=41.015625, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324256, Length=481, Percent_Identity=29.3139293139293, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6323011, Length=543, Percent_Identity=25.5985267034991, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321807, Length=467, Percent_Identity=22.9122055674518, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640849, Length=483, Percent_Identity=42.4430641821946, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640851, Length=511, Percent_Identity=40.9001956947162, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17136276, Length=469, Percent_Identity=24.3070362473348, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24584738, Length=287, Percent_Identity=25.7839721254355, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_EDWI9 (C5BAR6)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934710.1
- GeneID:   7959637
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3337
- OMA:   VDMIANP
- ProtClustDB:   PRK00484
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 57740; Mature: 57609

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQQQQGAEQALDLNNEMQARREKLAALRKEGIAFPNDFRRDTTSDKLHSLYDGKSKEE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHCCCCHHH
LEALNVEVSVAGRMMTRRIMGKASFVTLQDMGGRIQLYVARDDLKDDVYSEQFKKWDLGD
HHHHCEEHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHCCCCHHH
IIGARGTLFRTQTGELSIHCHSIHLLTKALRPLPDKFHGLSDQETRYRQRYLDLIANESS
HHCCCCCEEEECCCCEEEEEHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHCCCH
RETFRTRSKILAAIRNFMVNKGFMEVETPMMQVIPGGASARPFITHHNALDIDMYLRIAP
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHCCCCCCCCCEEEECCCEEHHHHHHHHH
ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTEELFRT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHH
ISQDVLGSSVVQYGDQTFDFGKPFIKMSMKEAICHYRPDIASADLDDMDKACKIAESLGI
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
KIEKSWGLGRVQCEIFDETAESQLIQPTFITEYPAEVSPLARRNDDNPFITDRFEFFIGG
EEEECCCCCEEEEEEECCHHHHHCCCCHHCCCCCCCCCHHHHCCCCCCEEECCHHEEECC
REIGNGFSELNDAEDQAERFQDQVKAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGVGID
HHHCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHCCCCCCCCCCCHH
RMMMLFTNSHTIRDVILFPAMRPQK
HHHHHHHCCCHHHHHHEECCCCCCC
>Mature Secondary Structure 
SEQQQQGAEQALDLNNEMQARREKLAALRKEGIAFPNDFRRDTTSDKLHSLYDGKSKEE
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHCCCCHHH
LEALNVEVSVAGRMMTRRIMGKASFVTLQDMGGRIQLYVARDDLKDDVYSEQFKKWDLGD
HHHHCEEHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHCCCCHHH
IIGARGTLFRTQTGELSIHCHSIHLLTKALRPLPDKFHGLSDQETRYRQRYLDLIANESS
HHCCCCCEEEECCCCEEEEEHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHCCCH
RETFRTRSKILAAIRNFMVNKGFMEVETPMMQVIPGGASARPFITHHNALDIDMYLRIAP
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHCCCCCCCCCEEEECCCEEHHHHHHHHH
ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTEELFRT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHH
ISQDVLGSSVVQYGDQTFDFGKPFIKMSMKEAICHYRPDIASADLDDMDKACKIAESLGI
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
KIEKSWGLGRVQCEIFDETAESQLIQPTFITEYPAEVSPLARRNDDNPFITDRFEFFIGG
EEEECCCCCEEEEEEECCHHHHHCCCCHHCCCCCCCCCHHHHCCCCCCEEECCHHEEECC
REIGNGFSELNDAEDQAERFQDQVKAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGVGID
HHHCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHCCCCCCCCCCCHH
RMMMLFTNSHTIRDVILFPAMRPQK
HHHHHHHCCCHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA