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The map label for this gene is tesA [H]

Identifier: 238920888

GI number: 238920888

Start: 2902284

End: 2902898

Strand: Direct

Name: tesA [H]

Synonym: NT01EI_3015

Alternate gene names: 238920888

Gene position: 2902284-2902898 (Clockwise)

Preceding gene: 238920885

Following gene: 238920889

Centisome position: 76.13

GC content: 67.64

Gene sequence:

>615_bases
GTGGTCATGATCCTGTGTGGCCTGACGGCAGCCCGTGCCGATACACTGCTGGTGCTGGGGGACAGCCTGAGCGCCGCCTA
TCGCCTGCCGGAGCAGCAGGGCTGGGTCGCACGTCTGGCACAGCAATGGCAGCGGCGTACCCCGCCGCTGACGGTGATCA
ACGCCAGCATCAGCGGCGATACCGCCGGGCAGGGCCTGCTGCGCCTGCCGGCGCTGCTGCAGCAGCACCGGCCGCGCTGG
GTGCTGATTGAGCTGGGGGCCAACGATGGGCTACGCGGCGTTGCTCCTGACGCCATCGCCGCGACGCTGTCCGCTGTCAT
CGGACAGGTGCAGCAGACTGGCGCCACGCCGCTGCTGATGCAGATCCGCCTGCCGCCCAACTACGGCCGGCGCTACGGCG
ACGCCTTTGCGGCTATCTACCCTCAGCTGGCAGCGCGCTTTAACGCTACACTGCTGCCGTTTTATATGGAGGCGGTGGTC
GGCCATCCCGACTGGATCCAGAATGACGGCCTGCACCCCAACGCGCTGGCCCAGCCATTTATCGCCGACTGGATGGCGCA
GCGCCTGCTGCCGCAGCTAACGCGCGCGGAGGACGGCGCGCCGTCTATTAATTAA

Upstream 100 bases:

>100_bases
CAGCAGTAAGGATGGAGATCTGGTTTTCACCCTCACCAACCCGTTTGGCGACATGATGAACTTCAATGACGTTTTGCGCT
GGCATCGGATACTCCTGATA

Downstream 100 bases:

>100_bases
ACATGAATATTCAGCGACTAACAGGGCAACTTAGGTAAACTTATGCAAAAAGCCATCTTAATCACCGGCTGTTCCAGCGG
CATCGGACTGGCGGCGGCCA

Product: acyl-CoA thioesterase I

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MVMILCGLTAARADTLLVLGDSLSAAYRLPEQQGWVARLAQQWQRRTPPLTVINASISGDTAGQGLLRLPALLQQHRPRW
VLIELGANDGLRGVAPDAIAATLSAVIGQVQQTGATPLLMQIRLPPNYGRRYGDAFAAIYPQLAARFNATLLPFYMEAVV
GHPDWIQNDGLHPNALAQPFIADWMAQRLLPQLTRAEDGAPSIN

Sequences:

>Translated_204_residues
MVMILCGLTAARADTLLVLGDSLSAAYRLPEQQGWVARLAQQWQRRTPPLTVINASISGDTAGQGLLRLPALLQQHRPRW
VLIELGANDGLRGVAPDAIAATLSAVIGQVQQTGATPLLMQIRLPPNYGRRYGDAFAAIYPQLAARFNATLLPFYMEAVV
GHPDWIQNDGLHPNALAQPFIADWMAQRLLPQLTRAEDGAPSIN
>Mature_204_residues
MVMILCGLTAARADTLLVLGDSLSAAYRLPEQQGWVARLAQQWQRRTPPLTVINASISGDTAGQGLLRLPALLQQHRPRW
VLIELGANDGLRGVAPDAIAATLSAVIGQVQQTGATPLLMQIRLPPNYGRRYGDAFAAIYPQLAARFNATLLPFYMEAVV
GHPDWIQNDGLHPNALAQPFIADWMAQRLLPQLTRAEDGAPSIN

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=191, Percent_Identity=59.1623036649215, Blast_Score=233, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 22162; Mature: 22162

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMILCGLTAARADTLLVLGDSLSAAYRLPEQQGWVARLAQQWQRRTPPLTVINASISGD
CEEEEECCCHHCCCEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
TAGQGLLRLPALLQQHRPRWVLIELGANDGLRGVAPDAIAATLSAVIGQVQQTGATPLLM
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
QIRLPPNYGRRYGDAFAAIYPQLAARFNATLLPFYMEAVVGHPDWIQNDGLHPNALAQPF
EEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
IADWMAQRLLPQLTRAEDGAPSIN
HHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVMILCGLTAARADTLLVLGDSLSAAYRLPEQQGWVARLAQQWQRRTPPLTVINASISGD
CEEEEECCCHHCCCEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
TAGQGLLRLPALLQQHRPRWVLIELGANDGLRGVAPDAIAATLSAVIGQVQQTGATPLLM
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
QIRLPPNYGRRYGDAFAAIYPQLAARFNATLLPFYMEAVVGHPDWIQNDGLHPNALAQPF
EEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
IADWMAQRLLPQLTRAEDGAPSIN
HHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]