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The map label for this gene is folD

Identifier: 238920876

GI number: 238920876

Start: 2889494

End: 2890351

Strand: Direct

Name: folD

Synonym: NT01EI_3003

Alternate gene names: 238920876

Gene position: 2889494-2890351 (Clockwise)

Preceding gene: 238920875

Following gene: 238920877

Centisome position: 75.79

GC content: 64.34

Gene sequence:

>858_bases
ATGGCAGCAAAAATTATTGATGGGAAAACGATTGCGCGACAAGTCAGAAGTGAAGTGGCGGCACGGGTGCGACAACGCCT
GGCGGACGGCAAACGGGCACCGGGACTGGCGGTCATCATGGTCGGTGACGATCCGGCCTCCCGGATCTACGTCGGCAGCA
AGCAGCGCGCCTGTGAAGAGGTCGGTTTTCTCTCCCGTTCCTATGCACTGCCGGACAGCACCGATGAGGCCCAGCTGCTT
GCGCTGATCGATACGCTCAACGCCGACGCGGCCATCGATGGCATTCTGGTACAGCTGCCGCTGCCCGCCGGCATCGATAA
CAGCCGGGTGCTGGAACGCATCCATCCGGACAAAGACGTGGATGGCTTTCACCCCTACAATCTGGGGCGCCTGTGCCAGC
GCACCCCCAAGCTCCGTCCCTGCACCCCGCGCGGCATCATTACCCTGCTGGAGCGCTGCGGCATCGAGACCCAGGGGATG
GATGCCGTGATGGTTGGCGCCTCCAACATCGTGGGAAGACCGATGGCGCTGGAGCTGCTGCTGGCCGGCTGCACCACCAC
CATCACCCACAGTCGCACCCGTAACCTGCAGCAGCACGTCGAGCGCGCCGATCTGATCGTCGCCGCCGTCGGTAAACCCG
GCTTTATCCCCGGCGAATGGGTCAAGCCCGGCGCAGTGGTGATCGATGTCGGCATCAATCGGCTGGAGAGCGGTAAGGTG
GTCGGCGACGTCGACTTTACCGGTGCCGCCCAGCGCGCCAGCTGGATCACCCCGGTCCCCGGCGGCGTCGGGCCGATGAC
CGTCGCCACCCTGATGCAGAACACCCTGCAGGCCTGTGAGGCGTTTCACGACGACTAA

Upstream 100 bases:

>100_bases
CATCAGGCCTTGATTAAGATCAATATTCACCTGACAGCAAGCCAGCCATTTGCGACAATAGTGCCATCCCCGCAACATAA
CCAGGATGGATCAACTCACC

Downstream 100 bases:

>100_bases
GAGCCGCTAACAAAACCGAGTTGATTCGAATAACATCAATTCCCATATAGTAGGCATGACACGAAAAATCATCAGTAAGC
AGCTCTGGAAATCCTTACAG

Product: bifunctional protein FolD

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MAAKIIDGKTIARQVRSEVAARVRQRLADGKRAPGLAVIMVGDDPASRIYVGSKQRACEEVGFLSRSYALPDSTDEAQLL
ALIDTLNADAAIDGILVQLPLPAGIDNSRVLERIHPDKDVDGFHPYNLGRLCQRTPKLRPCTPRGIITLLERCGIETQGM
DAVMVGASNIVGRPMALELLLAGCTTTITHSRTRNLQQHVERADLIVAAVGKPGFIPGEWVKPGAVVIDVGINRLESGKV
VGDVDFTGAAQRASWITPVPGGVGPMTVATLMQNTLQACEAFHDD

Sequences:

>Translated_285_residues
MAAKIIDGKTIARQVRSEVAARVRQRLADGKRAPGLAVIMVGDDPASRIYVGSKQRACEEVGFLSRSYALPDSTDEAQLL
ALIDTLNADAAIDGILVQLPLPAGIDNSRVLERIHPDKDVDGFHPYNLGRLCQRTPKLRPCTPRGIITLLERCGIETQGM
DAVMVGASNIVGRPMALELLLAGCTTTITHSRTRNLQQHVERADLIVAAVGKPGFIPGEWVKPGAVVIDVGINRLESGKV
VGDVDFTGAAQRASWITPVPGGVGPMTVATLMQNTLQACEAFHDD
>Mature_284_residues
AAKIIDGKTIARQVRSEVAARVRQRLADGKRAPGLAVIMVGDDPASRIYVGSKQRACEEVGFLSRSYALPDSTDEAQLLA
LIDTLNADAAIDGILVQLPLPAGIDNSRVLERIHPDKDVDGFHPYNLGRLCQRTPKLRPCTPRGIITLLERCGIETQGMD
AVMVGASNIVGRPMALELLLAGCTTTITHSRTRNLQQHVERADLIVAAVGKPGFIPGEWVKPGAVVIDVGINRLESGKVV
GDVDFTGAAQRASWITPVPGGVGPMTVATLMQNTLQACEAFHDD

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=45.3924914675768, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=41.8367346938776, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=43.0034129692833, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI36796743, Length=209, Percent_Identity=30.622009569378, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=76.7605633802817, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17568735, Length=290, Percent_Identity=39.6551724137931, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=45.3020134228188, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=41.7543859649123, Blast_Score=219, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6322933, Length=314, Percent_Identity=24.5222929936306, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17136816, Length=298, Percent_Identity=48.3221476510067, Blast_Score=245, Evalue=3e-65,
Organism=Drosophila melanogaster, GI17136818, Length=298, Percent_Identity=48.3221476510067, Blast_Score=244, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=43.1506849315069, Blast_Score=219, Evalue=1e-57,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=43.1506849315069, Blast_Score=219, Evalue=1e-57,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=43.1506849315069, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=43.1506849315069, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_EDWI9 (C5B8V3)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934391.1
- GeneID:   7959302
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3003
- OMA:   DVVYETA
- ProtClustDB:   CLSK2813526
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30471; Mature: 30339

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKIIDGKTIARQVRSEVAARVRQRLADGKRAPGLAVIMVGDDPASRIYVGSKQRACEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCHHHHHHH
VGFLSRSYALPDSTDEAQLLALIDTLNADAAIDGILVQLPLPAGIDNSRVLERIHPDKDV
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNLGRLCQRTPKLRPCTPRGIITLLERCGIETQGMDAVMVGASNIVGRPMALELL
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCHHHCCCHHHHHHH
LAGCTTTITHSRTRNLQQHVERADLIVAAVGKPGFIPGEWVKPGAVVIDVGINRLESGKV
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCE
VGDVDFTGAAQRASWITPVPGGVGPMTVATLMQNTLQACEAFHDD
EECCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAKIIDGKTIARQVRSEVAARVRQRLADGKRAPGLAVIMVGDDPASRIYVGSKQRACEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCHHHHHHH
VGFLSRSYALPDSTDEAQLLALIDTLNADAAIDGILVQLPLPAGIDNSRVLERIHPDKDV
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNLGRLCQRTPKLRPCTPRGIITLLERCGIETQGMDAVMVGASNIVGRPMALELL
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCHHHCCCHHHHHHH
LAGCTTTITHSRTRNLQQHVERADLIVAAVGKPGFIPGEWVKPGAVVIDVGINRLESGKV
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCE
VGDVDFTGAAQRASWITPVPGGVGPMTVATLMQNTLQACEAFHDD
EECCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA