Click here to switch to the map view.

The map label for this gene is gsp [H]

Identifier: 238920872

GI number: 238920872

Start: 2884064

End: 2885938

Strand: Direct

Name: gsp [H]

Synonym: NT01EI_2998

Alternate gene names: 238920872

Gene position: 2884064-2885938 (Clockwise)

Preceding gene: 238920864

Following gene: 238920875

Centisome position: 75.65

GC content: 56.59

Gene sequence:

>1875_bases
ATGAGCGTAGATATTACCCACAAGGACGCCCCTTTCGGTACTTTGCTTGGCTATGCGCCGGGCGGCGTCGCAATTTATTC
TTCTAACTATAGCACGCTGGATCCCCGGGTTTATCCCGATGAAGCCTCCCTGCGCAGCTACATCGACGACGAATATATGG
GCCATAAGTGGCAGTGCGTCGAGTTTGCCCGCCGTTTTCTTTTTCTGAACTATGGCGTCGTCTTTACCGACGTCGGCATG
GCCTATGAAATTTTCTCCCTGCGTTTTCTGCGCCAGGTCGTTAACGACAGCCTGCTGCCACTGCAGGCCTTCGCCAACGG
TTCGGCGCGGGCGCCGGTCGCCGGTGCTCTGCTTATCTGGCAAAAAGGCGGTGAGTTCAAGGATACCGGACACGTTGCCA
TCATCACCCAGCTGATGGGAGATCGGGTCCGCATCGCAGAGCAAAACGTTATCCACGCCCCGCTACCGCCGGGACAGCAG
TGGACGCGAGAGCTCACGCTTCAGCGACAGCATGGCCGCTATACCATTCTGGACTCATTTGACGATACCGAAATCCTCGG
CTGGATGATCCATACTGATGACGCCCACGGCAGTCTACCCCAGCCCTCCCTGGCACCACAGATGATGGCGCTGCATGGCG
CCCGGCGCAGCGATAAAAATACCTTTGACGGCCGCTGGCTGAATGAAAATGACCCACTGGAAAAAGCCTATGTACAGGCT
AATGGGCACGTCATCAACCAAAACCCGTATCAATATGTCACCATCAGCGAAAGCGCCGAGCAGGAGCTGATCAAGGCCAC
CAATGAGCTGCACCTGATGTACCTGCATGCTACAGATAAGGTGCTGCGCGATGACAGTCTGCTGGCGCTGTTCGATATTC
CCAAGATCCTCTGGCCACGTCTGCGCCTCTCTTGGCAACAGCGCCGCCACTGCATGATCACCGGACGCATGGATTTCTGT
ATGGATGAGCGAGGGCTGAAGGTCTACGAATATAATGCAGACTCGGCGTCCTGCCATACGGAGGCTGCGCTGATCCTGCA
ACGGTGGGCAAAGCAGGGCGACGGGGTTCCGGGATACAACCCGGGCGAGGATCTGCTCAATGAACTGGCCGGTGCCTGGC
GCCACAGTCATGCCCGCCCGTTTGTCCACATCATGCAGGATAACGATATTGAAGAGAGCTATCACGCGCTCTTTATGCAG
CGGACGCTATCGCAGGCCGGTTTCGATAGCAAGATAGTCAAAGGGCTGGCATCGCTGCGCTGGGACACCACCGGTCAACT
TATCGACGAGGATGGACGCTTGGTTAACTGCGTTTGGAAAACCTGGGCGTGGGAAACGGCCCTGGAGCAGGTGCGGGAGG
TCAGCGACACCGAATATGCCGCAGTGCCAATCCGCACCGGCCAGCCGGAAAAGCAGGTGCGGCTGATCGACGTTCTGCTG
CGTCCTGAAATCATGGTGTTCGAACCGCTGTGGACCGTCATCCCCGGTAACAAGGCGATCCTCCCGATCCTGTGGTCGCT
GTTCCCCCACCACCGCTATCTGTTGGATACCGATTTTGAGCCGACGCCGGAGCTGATCCGCAGCGGCTATGCCGTTAAGC
CGATAGCCGGACGCTGTGGCAGTAATATCGATCTGGTCAGCCGCCATGAGGAGGTGCTCGATACCACCAGCGGGAAATTC
CACGATCAGAAAAATATTTACCAACAGCTATGGTGCCTGCCACAGGTAGCGGACAAACATATCCAGGTCTGTACCTTTAC
CGTAGGCGGTAGCTACGGCGGTGCCTGCCTGCGCGCAGACAGTTCTTTGGTGATCAAGAAAGAGAGTGATATCGAACCGC
TTATCGTGCTCAAAGACAGTGATTTTCTGAAGTAA

Upstream 100 bases:

>100_bases
TGAGTTCACCTCTGTCTGGCTGGCGCCAGCAACTTGCCATGATGGCACCCTTTTCAACCCTGCCAGTCAGCTCCAGCAGC
GTTATACTGAGGTCAGGATC

Downstream 100 bases:

>100_bases
ACCACACCTGCCCAGCCCGCCACATTCGGCGGGCTTAAGCGCGACAGAGAGCCTGCTCCAGCAAAAGACTGGCGATAACA
CACGCCGGGCCGCGCAGAGA

Product: bifunctional glutathionylspermidine amidase/glutathionylspermidine synthetase

Products: NA

Alternate protein names: Glutathionylspermidine synthase; GSP synthetase; Glutathione:spermidine ligase [ADP-forming]; Glutathionylspermidine amidase; Glutathionylspermidine amidohydrolase [spermidine-forming]; GSP amidase [H]

Number of amino acids: Translated: 624; Mature: 623

Protein sequence:

>624_residues
MSVDITHKDAPFGTLLGYAPGGVAIYSSNYSTLDPRVYPDEASLRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGM
AYEIFSLRFLRQVVNDSLLPLQAFANGSARAPVAGALLIWQKGGEFKDTGHVAIITQLMGDRVRIAEQNVIHAPLPPGQQ
WTRELTLQRQHGRYTILDSFDDTEILGWMIHTDDAHGSLPQPSLAPQMMALHGARRSDKNTFDGRWLNENDPLEKAYVQA
NGHVINQNPYQYVTISESAEQELIKATNELHLMYLHATDKVLRDDSLLALFDIPKILWPRLRLSWQQRRHCMITGRMDFC
MDERGLKVYEYNADSASCHTEAALILQRWAKQGDGVPGYNPGEDLLNELAGAWRHSHARPFVHIMQDNDIEESYHALFMQ
RTLSQAGFDSKIVKGLASLRWDTTGQLIDEDGRLVNCVWKTWAWETALEQVREVSDTEYAAVPIRTGQPEKQVRLIDVLL
RPEIMVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFEPTPELIRSGYAVKPIAGRCGSNIDLVSRHEEVLDTTSGKF
HDQKNIYQQLWCLPQVADKHIQVCTFTVGGSYGGACLRADSSLVIKKESDIEPLIVLKDSDFLK

Sequences:

>Translated_624_residues
MSVDITHKDAPFGTLLGYAPGGVAIYSSNYSTLDPRVYPDEASLRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGM
AYEIFSLRFLRQVVNDSLLPLQAFANGSARAPVAGALLIWQKGGEFKDTGHVAIITQLMGDRVRIAEQNVIHAPLPPGQQ
WTRELTLQRQHGRYTILDSFDDTEILGWMIHTDDAHGSLPQPSLAPQMMALHGARRSDKNTFDGRWLNENDPLEKAYVQA
NGHVINQNPYQYVTISESAEQELIKATNELHLMYLHATDKVLRDDSLLALFDIPKILWPRLRLSWQQRRHCMITGRMDFC
MDERGLKVYEYNADSASCHTEAALILQRWAKQGDGVPGYNPGEDLLNELAGAWRHSHARPFVHIMQDNDIEESYHALFMQ
RTLSQAGFDSKIVKGLASLRWDTTGQLIDEDGRLVNCVWKTWAWETALEQVREVSDTEYAAVPIRTGQPEKQVRLIDVLL
RPEIMVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFEPTPELIRSGYAVKPIAGRCGSNIDLVSRHEEVLDTTSGKF
HDQKNIYQQLWCLPQVADKHIQVCTFTVGGSYGGACLRADSSLVIKKESDIEPLIVLKDSDFLK
>Mature_623_residues
SVDITHKDAPFGTLLGYAPGGVAIYSSNYSTLDPRVYPDEASLRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMA
YEIFSLRFLRQVVNDSLLPLQAFANGSARAPVAGALLIWQKGGEFKDTGHVAIITQLMGDRVRIAEQNVIHAPLPPGQQW
TRELTLQRQHGRYTILDSFDDTEILGWMIHTDDAHGSLPQPSLAPQMMALHGARRSDKNTFDGRWLNENDPLEKAYVQAN
GHVINQNPYQYVTISESAEQELIKATNELHLMYLHATDKVLRDDSLLALFDIPKILWPRLRLSWQQRRHCMITGRMDFCM
DERGLKVYEYNADSASCHTEAALILQRWAKQGDGVPGYNPGEDLLNELAGAWRHSHARPFVHIMQDNDIEESYHALFMQR
TLSQAGFDSKIVKGLASLRWDTTGQLIDEDGRLVNCVWKTWAWETALEQVREVSDTEYAAVPIRTGQPEKQVRLIDVLLR
PEIMVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFEPTPELIRSGYAVKPIAGRCGSNIDLVSRHEEVLDTTSGKFH
DQKNIYQQLWCLPQVADKHIQVCTFTVGGSYGGACLRADSSLVIKKESDIEPLIVLKDSDFLK

Specific function: Catalyzes the formation of an amide bond between glutathione and spermidine coupled with hydrolysis of ATP; also catalyzes the hydrolysis of glutathionylspermidine to glutathione and spermidine [H]

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase C51 domain [H]

Homologues:

Organism=Escherichia coli, GI1789361, Length=618, Percent_Identity=78.6407766990291, Blast_Score=1035, Evalue=0.0,
Organism=Escherichia coli, GI1790629, Length=384, Percent_Identity=26.5625, Blast_Score=123, Evalue=5e-29,
Organism=Escherichia coli, GI1789416, Length=391, Percent_Identity=25.0639386189258, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007921
- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF05257 CHAP; PF03738 GSP_synth [H]

EC number: =6.3.1.8; =3.5.1.78 [H]

Molecular weight: Translated: 70880; Mature: 70749

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDITHKDAPFGTLLGYAPGGVAIYSSNYSTLDPRVYPDEASLRSYIDDEYMGHKWQCV
CCEECCCCCCCCHHHEECCCCCEEEEECCCCCCCCEECCCHHHHHHHCCHHHCCCCHHHH
EFARRFLFLNYGVVFTDVGMAYEIFSLRFLRQVVNDSLLPLQAFANGSARAPVAGALLIW
HHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEEEEE
QKGGEFKDTGHVAIITQLMGDRVRIAEQNVIHAPLPPGQQWTRELTLQRQHGRYTILDSF
ECCCCCCCCCCEEEEEHHHCCCEEEECCCEEECCCCCCHHHHHHHHHHHCCCCEEEECCC
DDTEILGWMIHTDDAHGSLPQPSLAPQMMALHGARRSDKNTFDGRWLNENDPLEKAYVQA
CCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEEEE
NGHVINQNPYQYVTISESAEQELIKATNELHLMYLHATDKVLRDDSLLALFDIPKILWPR
CCEEECCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCHHHCCCCEEEEECCHHHHHHH
LRLSWQQRRHCMITGRMDFCMDERGLKVYEYNADSASCHTEAALILQRWAKQGDGVPGYN
HHHCHHCCCEEEEECCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PGEDLLNELAGAWRHSHARPFVHIMQDNDIEESYHALFMQRTLSQAGFDSKIVKGLASLR
CHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
WDTTGQLIDEDGRLVNCVWKTWAWETALEQVREVSDTEYAAVPIRTGQPEKQVRLIDVLL
CCCCCCEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
RPEIMVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFEPTPELIRSGYAVKPIAGRCG
CCCEEEECCCCEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCCCCHHCCC
SNIDLVSRHEEVLDTTSGKFHDQKNIYQQLWCLPQVADKHIQVCTFTVGGSYGGACLRAD
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHCCCCEEEEEEEECCCCCCEEEECC
SSLVIKKESDIEPLIVLKDSDFLK
CCEEEEECCCCCCEEEECCCCCCC
>Mature Secondary Structure 
SVDITHKDAPFGTLLGYAPGGVAIYSSNYSTLDPRVYPDEASLRSYIDDEYMGHKWQCV
CEECCCCCCCCHHHEECCCCCEEEEECCCCCCCCEECCCHHHHHHHCCHHHCCCCHHHH
EFARRFLFLNYGVVFTDVGMAYEIFSLRFLRQVVNDSLLPLQAFANGSARAPVAGALLIW
HHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEEEEE
QKGGEFKDTGHVAIITQLMGDRVRIAEQNVIHAPLPPGQQWTRELTLQRQHGRYTILDSF
ECCCCCCCCCCEEEEEHHHCCCEEEECCCEEECCCCCCHHHHHHHHHHHCCCCEEEECCC
DDTEILGWMIHTDDAHGSLPQPSLAPQMMALHGARRSDKNTFDGRWLNENDPLEKAYVQA
CCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEEEE
NGHVINQNPYQYVTISESAEQELIKATNELHLMYLHATDKVLRDDSLLALFDIPKILWPR
CCEEECCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCHHHCCCCEEEEECCHHHHHHH
LRLSWQQRRHCMITGRMDFCMDERGLKVYEYNADSASCHTEAALILQRWAKQGDGVPGYN
HHHCHHCCCEEEEECCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PGEDLLNELAGAWRHSHARPFVHIMQDNDIEESYHALFMQRTLSQAGFDSKIVKGLASLR
CHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
WDTTGQLIDEDGRLVNCVWKTWAWETALEQVREVSDTEYAAVPIRTGQPEKQVRLIDVLL
CCCCCCEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
RPEIMVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFEPTPELIRSGYAVKPIAGRCG
CCCEEEECCCCEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCCCCHHCCC
SNIDLVSRHEEVLDTTSGKFHDQKNIYQQLWCLPQVADKHIQVCTFTVGGSYGGACLRAD
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHCCCCEEEEEEEECCCCCCEEEECC
SSLVIKKESDIEPLIVLKDSDFLK
CCEEEEECCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7775463; 9278503 [H]