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The map label for this gene is dacA [H]

Identifier: 238920819

GI number: 238920819

Start: 2833893

End: 2835104

Strand: Direct

Name: dacA [H]

Synonym: NT01EI_2945

Alternate gene names: 238920819

Gene position: 2833893-2835104 (Clockwise)

Preceding gene: 238920818

Following gene: 238920820

Centisome position: 74.34

GC content: 60.07

Gene sequence:

>1212_bases
ATGAAACGCAAAGTTGCTTCCCAAACACTGAAATACGCTGTCGCCGGCGGTCTGCTGGCCATAGCGCTCACCGGTGCGGC
CCGCGCCGACGACGTTAACCTGAAGACCATGATCCCGGGCGTGCCGCAAATCGATGCCGAAGCCTATATTCTGATCGACT
ACAACTCCGGTAAGGTGTTGGCGGAGCACAACGCCGACGAGCGCCGCAATCCGGCCTCGCTGACCAAGATGATGACCAGC
TACGTCATCGGACAGGCAATGCGCGCGGGCAAGTTCGGTGAGAACGACGTCGTCACCGTCGGCAAAGACGCCTGGGCGAC
GGGAAACCCGGTGTTCCAGGGCTCCTCGCTGATGTTCCTCAAGCCGGGCGATCAGGTCTCCGTGGGCAACCTGATCCGCG
GGATCAACCTGGTCTCCGGTAACGATGCCTGCGTCGCCATGGCCGACTATGTCGCCGGCAGCCAGGACGCCTTCGTCGGC
CTGATGAACAGCTACGTGAAGGCGCTGGGTCTGCAGAACACCCACTTTGAGACGGTACACGGCCTGGACGCGCCGGGGCA
GTTCAGCTCCGCCCGCGACATGGCACTGATCGGCCAGGCGCTGATCCGCGACGTCCCCCATGAGTACGCCATCTACAAAG
AGAAAGAGTTCACCTATAACAATATCCGTCAGACCAACCGTAACGGTCTGCTGTGGGATACCAGTATGAACGTGGACGGC
ATCAAAACCGGCCATACCGCCTCTGCGGGCTATAACCTGGTAGCCTCGGCGACCGAGGGCAATATGCGCCTGATCTCCGC
CGTCATGGGCGGGCGCACCTTTAAGGGGCGCGAGACCGAGAGCAAGAAGCTGCTGACCTGGGGCTTCCGCTTCTTTGAAA
CCGTCTCGCCGCTGAAGGCCGGGCGTGAGTTCGCCTCTGAACCGGTCTGGTTCGGTGACACCGACCGCGCTCAGCTGGGT
GTCGATAAAGATGTCTACCTGACCATCCCGCGCGGACGGATGAAAGATCTCAAGGCCAGCTACGTGCTGAACAGCAACGA
ACTGGATGCTCCGCTGGCCAAGGATCAGGTGGTCGGTACCATCAACTTCCAGCTGGACGGCAAGACCATCGAACAGCGCC
CGCTGGTGGTGCTGAACGAAGTGAAAGAGGGTGGTTTCTTCAGCCGGATGATCGATCATATCAAGCTGCTGTTTCATCGC
TGGTTTGGTTAA

Upstream 100 bases:

>100_bases
ATTGTGTAAAAAACCATTGCCACGCTATGCCTTAGCGTACCATCTGCTATAGTGTGGCTCGTTTGTCACTGTTTAATTCA
CGGATGTTGTAGTCCCCATC

Downstream 100 bases:

>100_bases
TCTGTGGCCAGCCTCTTGAAAAGGGCATCGACGGCCCCATTTAATCGGTAAGTAAGGCTCCCGCTACGGCGGGAGTTATA
ATTTGTAGATAGTGCAATAC

Product: D-alanyl-D-alanine carboxypeptidase fraction A

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKRKVASQTLKYAVAGGLLAIALTGAARADDVNLKTMIPGVPQIDAEAYILIDYNSGKVLAEHNADERRNPASLTKMMTS
YVIGQAMRAGKFGENDVVTVGKDAWATGNPVFQGSSLMFLKPGDQVSVGNLIRGINLVSGNDACVAMADYVAGSQDAFVG
LMNSYVKALGLQNTHFETVHGLDAPGQFSSARDMALIGQALIRDVPHEYAIYKEKEFTYNNIRQTNRNGLLWDTSMNVDG
IKTGHTASAGYNLVASATEGNMRLISAVMGGRTFKGRETESKKLLTWGFRFFETVSPLKAGREFASEPVWFGDTDRAQLG
VDKDVYLTIPRGRMKDLKASYVLNSNELDAPLAKDQVVGTINFQLDGKTIEQRPLVVLNEVKEGGFFSRMIDHIKLLFHR
WFG

Sequences:

>Translated_403_residues
MKRKVASQTLKYAVAGGLLAIALTGAARADDVNLKTMIPGVPQIDAEAYILIDYNSGKVLAEHNADERRNPASLTKMMTS
YVIGQAMRAGKFGENDVVTVGKDAWATGNPVFQGSSLMFLKPGDQVSVGNLIRGINLVSGNDACVAMADYVAGSQDAFVG
LMNSYVKALGLQNTHFETVHGLDAPGQFSSARDMALIGQALIRDVPHEYAIYKEKEFTYNNIRQTNRNGLLWDTSMNVDG
IKTGHTASAGYNLVASATEGNMRLISAVMGGRTFKGRETESKKLLTWGFRFFETVSPLKAGREFASEPVWFGDTDRAQLG
VDKDVYLTIPRGRMKDLKASYVLNSNELDAPLAKDQVVGTINFQLDGKTIEQRPLVVLNEVKEGGFFSRMIDHIKLLFHR
WFG
>Mature_403_residues
MKRKVASQTLKYAVAGGLLAIALTGAARADDVNLKTMIPGVPQIDAEAYILIDYNSGKVLAEHNADERRNPASLTKMMTS
YVIGQAMRAGKFGENDVVTVGKDAWATGNPVFQGSSLMFLKPGDQVSVGNLIRGINLVSGNDACVAMADYVAGSQDAFVG
LMNSYVKALGLQNTHFETVHGLDAPGQFSSARDMALIGQALIRDVPHEYAIYKEKEFTYNNIRQTNRNGLLWDTSMNVDG
IKTGHTASAGYNLVASATEGNMRLISAVMGGRTFKGRETESKKLLTWGFRFFETVSPLKAGREFASEPVWFGDTDRAQLG
VDKDVYLTIPRGRMKDLKASYVLNSNELDAPLAKDQVVGTINFQLDGKTIEQRPLVVLNEVKEGGFFSRMIDHIKLLFHR
WFG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=385, Percent_Identity=84.1558441558442, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1787062, Length=362, Percent_Identity=66.2983425414365, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI87082022, Length=394, Percent_Identity=47.7157360406091, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI87082054, Length=260, Percent_Identity=26.1538461538462, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44118; Mature: 44118

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKVASQTLKYAVAGGLLAIALTGAARADDVNLKTMIPGVPQIDAEAYILIDYNSGKVL
CCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEECCCCCCCCCCEEEEEEECCCEEE
AEHNADERRNPASLTKMMTSYVIGQAMRAGKFGENDVVTVGKDAWATGNPVFQGSSLMFL
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEECCCEEEEE
KPGDQVSVGNLIRGINLVSGNDACVAMADYVAGSQDAFVGLMNSYVKALGLQNTHFETVH
CCCCCCCHHHHHHCCEEECCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHCC
GLDAPGQFSSARDMALIGQALIRDVPHEYAIYKEKEFTYNNIRQTNRNGLLWDTSMNVDG
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHCCCCCEEEECCCCCCC
IKTGHTASAGYNLVASATEGNMRLISAVMGGRTFKGRETESKKLLTWGFRFFETVSPLKA
CCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GREFASEPVWFGDTDRAQLGVDKDVYLTIPRGRMKDLKASYVLNSNELDAPLAKDQVVGT
HHHHHCCCCCCCCCCCHHCCCCCCEEEEECCCCHHHCCHHEEECCCCCCCCCCCCCEEEE
INFQLDGKTIEQRPLVVLNEVKEGGFFSRMIDHIKLLFHRWFG
EEEEECCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRKVASQTLKYAVAGGLLAIALTGAARADDVNLKTMIPGVPQIDAEAYILIDYNSGKVL
CCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEECCCCCCCCCCEEEEEEECCCEEE
AEHNADERRNPASLTKMMTSYVIGQAMRAGKFGENDVVTVGKDAWATGNPVFQGSSLMFL
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEECCCEEEEE
KPGDQVSVGNLIRGINLVSGNDACVAMADYVAGSQDAFVGLMNSYVKALGLQNTHFETVH
CCCCCCCHHHHHHCCEEECCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHCC
GLDAPGQFSSARDMALIGQALIRDVPHEYAIYKEKEFTYNNIRQTNRNGLLWDTSMNVDG
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHCCCCCEEEECCCCCCC
IKTGHTASAGYNLVASATEGNMRLISAVMGGRTFKGRETESKKLLTWGFRFFETVSPLKA
CCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GREFASEPVWFGDTDRAQLGVDKDVYLTIPRGRMKDLKASYVLNSNELDAPLAKDQVVGT
HHHHHCCCCCCCCCCCHHCCCCCCEEEEECCCCHHHCCHHEEECCCCCCCCCCCCCEEEE
INFQLDGKTIEQRPLVVLNEVKEGGFFSRMIDHIKLLFHRWFG
EEEEECCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]