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The map label for this gene is birA [H]

Identifier: 238917596

GI number: 238917596

Start: 1734122

End: 1735135

Strand: Reverse

Name: birA [H]

Synonym: EUBELI_01675

Alternate gene names: 238917596

Gene position: 1735135-1734122 (Counterclockwise)

Preceding gene: 238917597

Following gene: 238917595

Centisome position: 80.92

GC content: 42.11

Gene sequence:

>1014_bases
ATGAAGACTAAGATTTTAAATGTTTTGAGAAAATCAGATGGATATGTGTCAGGGCAGGCACTGTGTAATATGCTGGGGGT
TTCAAGAACGGCTGTCTGGAAGGTGATAAACCAGTTAAAAGAAGAAGGCTATGTTATAGAGGCAGTCCAGAATAAGGGAT
ATCATATAACACAGTATCCTGATATTCTTACATCAAGCGAGATAATGAGTCAGCTTACATGTGTGGATACAGGCAGTACA
ACCGGGATTATCCGCGAAGTAGTGTATTATGATGAGACAGATTCTACCAACAACGAAGCTAAGAGAGCGGCAGAAAGAGA
TAATGCAGCTGATGGAACACTTTATATAACAGAGAGCCAGACAGGCGGCCGCGGAAGACGTGGCAGGAATTGGGTGTCTC
CTGCAGGAAGCGGGATATGGATGTCCCTTCTTTTAAGACCGGATATTGCACCAGTTAATGCGTCAATGCTTACAATAGTG
GCAGCCATGGCTGTGCAGGAGGCGATCCACACAGTTCTTAAAGAGGACGGACATGACGCAGAGTGCCGTATAAAGTGGCC
TAATGATATAGTTCTTAATAAGAAGAAGGTATGTGGAATATTAACAGAGATGAGTGCTGAGATGGATTACATACATTATG
TGGTTATCGGCATGGGAATTAATGTTAATACGACAGAATTTGATGATTCAATCAAGGCAACAGCTTCTTCATTGTATCTT
GAGACTGGCGACCATCTGAAAAGAAGCCGTATAGTAGCCGCATTTTCAGAGAGCTTTGCAAAGTACTATGATACATTTGT
TAAGACACAGAATCTGGCTGGGCTTAAAGAAGACTATAATTCAATGCTTGTCAATAAGGGCGGCGACGTCAAGGCAATAT
ATGCTGACAAGGAAATTGTTGGAAAAGCAATTGGAATCAATGATGAGGGTGAGCTTATCATTAAGACAGACGAGGGCGAG
AAGATTATCAGAACCGGAGAGGTTTCTGTGAGAGGATTATATGGATATGTTTGA

Upstream 100 bases:

>100_bases
CTAAGAATTTCATGAGCGACAGAACGGTGTCAGGTGTTATTCTTACAGTTGTTACGTTAGGACTTCTGCTTATGGCAGTA
ATAGCATGGCGGGTAATGGT

Downstream 100 bases:

>100_bases
TGAAAATGTAGTTTTATGTGTATCTAATTCTTATGAGAAGAAATACTATTTTAACCCTGATTTCGATAAGCTTCCGGATG
ATGTAAAGAACGAGTTAAAG

Product: BirA family transcriptional regulator

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKTKILNVLRKSDGYVSGQALCNMLGVSRTAVWKVINQLKEEGYVIEAVQNKGYHITQYPDILTSSEIMSQLTCVDTGST
TGIIREVVYYDETDSTNNEAKRAAERDNAADGTLYITESQTGGRGRRGRNWVSPAGSGIWMSLLLRPDIAPVNASMLTIV
AAMAVQEAIHTVLKEDGHDAECRIKWPNDIVLNKKKVCGILTEMSAEMDYIHYVVIGMGINVNTTEFDDSIKATASSLYL
ETGDHLKRSRIVAAFSESFAKYYDTFVKTQNLAGLKEDYNSMLVNKGGDVKAIYADKEIVGKAIGINDEGELIIKTDEGE
KIIRTGEVSVRGLYGYV

Sequences:

>Translated_337_residues
MKTKILNVLRKSDGYVSGQALCNMLGVSRTAVWKVINQLKEEGYVIEAVQNKGYHITQYPDILTSSEIMSQLTCVDTGST
TGIIREVVYYDETDSTNNEAKRAAERDNAADGTLYITESQTGGRGRRGRNWVSPAGSGIWMSLLLRPDIAPVNASMLTIV
AAMAVQEAIHTVLKEDGHDAECRIKWPNDIVLNKKKVCGILTEMSAEMDYIHYVVIGMGINVNTTEFDDSIKATASSLYL
ETGDHLKRSRIVAAFSESFAKYYDTFVKTQNLAGLKEDYNSMLVNKGGDVKAIYADKEIVGKAIGINDEGELIIKTDEGE
KIIRTGEVSVRGLYGYV
>Mature_337_residues
MKTKILNVLRKSDGYVSGQALCNMLGVSRTAVWKVINQLKEEGYVIEAVQNKGYHITQYPDILTSSEIMSQLTCVDTGST
TGIIREVVYYDETDSTNNEAKRAAERDNAADGTLYITESQTGGRGRRGRNWVSPAGSGIWMSLLLRPDIAPVNASMLTIV
AAMAVQEAIHTVLKEDGHDAECRIKWPNDIVLNKKKVCGILTEMSAEMDYIHYVVIGMGINVNTTEFDDSIKATASSLYL
ETGDHLKRSRIVAAFSESFAKYYDTFVKTQNLAGLKEDYNSMLVNKGGDVKAIYADKEIVGKAIGINDEGELIIKTDEGE
KIIRTGEVSVRGLYGYV

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=320, Percent_Identity=31.875, Blast_Score=138, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 37135; Mature: 37135

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKILNVLRKSDGYVSGQALCNMLGVSRTAVWKVINQLKEEGYVIEAVQNKGYHITQYP
CCHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCC
DILTSSEIMSQLTCVDTGSTTGIIREVVYYDETDSTNNEAKRAAERDNAADGTLYITESQ
CHHHHHHHHHHHEEECCCCCHHHHHHHHEECCCCCCCHHHHHHHHCCCCCCCEEEEEECC
TGGRGRRGRNWVSPAGSGIWMSLLLRPDIAPVNASMLTIVAAMAVQEAIHTVLKEDGHDA
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
ECRIKWPNDIVLNKKKVCGILTEMSAEMDYIHYVVIGMGINVNTTEFDDSIKATASSLYL
EEEEECCCCEEECCHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCHHHHHHHHHEE
ETGDHLKRSRIVAAFSESFAKYYDTFVKTQNLAGLKEDYNSMLVNKGGDVKAIYADKEIV
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECHHHH
GKAIGINDEGELIIKTDEGEKIIRTGEVSVRGLYGYV
HHEECCCCCCCEEEEECCCCEEEEECCEEEEEEECCC
>Mature Secondary Structure
MKTKILNVLRKSDGYVSGQALCNMLGVSRTAVWKVINQLKEEGYVIEAVQNKGYHITQYP
CCHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCC
DILTSSEIMSQLTCVDTGSTTGIIREVVYYDETDSTNNEAKRAAERDNAADGTLYITESQ
CHHHHHHHHHHHEEECCCCCHHHHHHHHEECCCCCCCHHHHHHHHCCCCCCCEEEEEECC
TGGRGRRGRNWVSPAGSGIWMSLLLRPDIAPVNASMLTIVAAMAVQEAIHTVLKEDGHDA
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
ECRIKWPNDIVLNKKKVCGILTEMSAEMDYIHYVVIGMGINVNTTEFDDSIKATASSLYL
EEEEECCCCEEECCHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCHHHHHHHHHEE
ETGDHLKRSRIVAAFSESFAKYYDTFVKTQNLAGLKEDYNSMLVNKGGDVKAIYADKEIV
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECHHHH
GKAIGINDEGELIIKTDEGEKIIRTGEVSVRGLYGYV
HHEECCCCCCCEEEEECCCCEEEEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]