Click here to switch to the map view.

The map label for this gene is gdh [H]

Identifier: 238917538

GI number: 238917538

Start: 1672241

End: 1673599

Strand: Reverse

Name: gdh [H]

Synonym: EUBELI_01617

Alternate gene names: 238917538

Gene position: 1673599-1672241 (Counterclockwise)

Preceding gene: 238917540

Following gene: 238917537

Centisome position: 78.05

GC content: 42.68

Gene sequence:

>1359_bases
ATGGGGTATGTTGATGAAGTATTAGACATCGTGTCTAAGAAGAATGCAGATCAGCCTGAATTTTTACAGGCAGTAACAGA
GGTTCTTGAGTCTTTAAGACCAGTCGTTGAGGCTAACGAGGAAGTTTACAGAAAGAACGCTATTCTTGAAAGAATTACAG
AGCCAGATCGTCAGATTATGTTCAGAGTTCCTTGGGTAGATGACAAGGGACAGGTACAGGTAAACAGAGGTTTCCGTGTA
CAGTTCAATAACGCTATTGGACCATACAAGGGTGGTTTAAGATTACATCCTTCAGTAAACTTAGGCATCATCAAGTTCTT
AGGTTTCGAACAGGTATTCAAGAACTCACTTACAACTCTTCCAATCGGTGGTGGTAAGGGTGGTTCTGATTTTGATCCTA
AGGGCAAGTCAGACAGAGAAATCATGGCATTCTGCCAGAGCTTTATGACAGAACTTTGCAAATATATCGGTGCTGATGTC
GATGTTCCAGCTGGTGATATCGGAACAGGCGCAAGAGAAATCGGATTCATGTTCGGTCAGTATAAGAGAATCCGTGGAAT
GTTCGAAGGCGTACTTACAGGTAAGGGACTTACATACGGTGGATCACTTGCAAGAACAGAAGCTACAGGTTATGGTTTAT
TATACCTTACTAATGCTTTATGGAAAGATAATGGAATGTCACTTGAGGGCAAGACAGCAGCTGTATCTGGTTCAGGTAAT
GTTGCTATCTACGCTATCCAGAAGGCACAGCAGTTAGGTGTTAAGGTTGTTACATGCTCAGACTCAACAGGCTGGATTTA
TGATCCAGAAGGAATTGATGTTGCACTTCTTAAGGAAGTTAAGGAAGTTAAGAGAGCACGTCTTACAGAATATGCTGCTG
CTAAGCCATCAGCTCAGTATTTTGCTAAGCAGAATGGTGAACACGGAGTATGGCAGTACAAGGTAGATCTTGCTCTTCCA
TGTGCTACACAGAATGAGTTAGACATCGAGGATGCTAAGATGCTTGTTGCTAACGGTGTTGTATCTGTAACAGAGGGTGC
TAACATGCCTACAACATTAGAAGCTACTAAGTATCTCCAGGAGAATGGTGTTCTCTTCGTAGGTGGTAAGGCTGCTAACG
CTGGTGGTGTTGCTACATCTGCTCTTGAGATGAGCCAGAATTCAGAGAGACTTTCATGGACATTCGAAGAAGTTGATGGA
AAGCTTAAGGGAATCATGGAAACAATCTACGCTAACATTTCAGATGCTGCTAAGAGATATGATATGACAGTTGGTGGAAA
GACAGACTATGTTGCTGGTGCTAATATCGCTGGTTTCGAGAAGGTTGTTGACGCTATGCTTGCTCAGGGAGTTTGCTAA

Upstream 100 bases:

>100_bases
AAAACTTGCGGAAATTAACCAGAACAGATATAATAACGCTAGAATCGATGTGCAGTAATGTACAATCTTAAATAATAATT
TAAAGGAGTGTTTTAAGGTT

Downstream 100 bases:

>100_bases
TTACAGGCTTTAATGTAATAATTGTCTTATAATATGATTTAATTGTAAGGTTCTGCAGACAATATGTCTGTAGAACCTTT
TTTTACTTGGCAGATTGCGA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MGYVDEVLDIVSKKNADQPEFLQAVTEVLESLRPVVEANEEVYRKNAILERITEPDRQIMFRVPWVDDKGQVQVNRGFRV
QFNNAIGPYKGGLRLHPSVNLGIIKFLGFEQVFKNSLTTLPIGGGKGGSDFDPKGKSDREIMAFCQSFMTELCKYIGADV
DVPAGDIGTGAREIGFMFGQYKRIRGMFEGVLTGKGLTYGGSLARTEATGYGLLYLTNALWKDNGMSLEGKTAAVSGSGN
VAIYAIQKAQQLGVKVVTCSDSTGWIYDPEGIDVALLKEVKEVKRARLTEYAAAKPSAQYFAKQNGEHGVWQYKVDLALP
CATQNELDIEDAKMLVANGVVSVTEGANMPTTLEATKYLQENGVLFVGGKAANAGGVATSALEMSQNSERLSWTFEEVDG
KLKGIMETIYANISDAAKRYDMTVGGKTDYVAGANIAGFEKVVDAMLAQGVC

Sequences:

>Translated_452_residues
MGYVDEVLDIVSKKNADQPEFLQAVTEVLESLRPVVEANEEVYRKNAILERITEPDRQIMFRVPWVDDKGQVQVNRGFRV
QFNNAIGPYKGGLRLHPSVNLGIIKFLGFEQVFKNSLTTLPIGGGKGGSDFDPKGKSDREIMAFCQSFMTELCKYIGADV
DVPAGDIGTGAREIGFMFGQYKRIRGMFEGVLTGKGLTYGGSLARTEATGYGLLYLTNALWKDNGMSLEGKTAAVSGSGN
VAIYAIQKAQQLGVKVVTCSDSTGWIYDPEGIDVALLKEVKEVKRARLTEYAAAKPSAQYFAKQNGEHGVWQYKVDLALP
CATQNELDIEDAKMLVANGVVSVTEGANMPTTLEATKYLQENGVLFVGGKAANAGGVATSALEMSQNSERLSWTFEEVDG
KLKGIMETIYANISDAAKRYDMTVGGKTDYVAGANIAGFEKVVDAMLAQGVC
>Mature_451_residues
GYVDEVLDIVSKKNADQPEFLQAVTEVLESLRPVVEANEEVYRKNAILERITEPDRQIMFRVPWVDDKGQVQVNRGFRVQ
FNNAIGPYKGGLRLHPSVNLGIIKFLGFEQVFKNSLTTLPIGGGKGGSDFDPKGKSDREIMAFCQSFMTELCKYIGADVD
VPAGDIGTGAREIGFMFGQYKRIRGMFEGVLTGKGLTYGGSLARTEATGYGLLYLTNALWKDNGMSLEGKTAAVSGSGNV
AIYAIQKAQQLGVKVVTCSDSTGWIYDPEGIDVALLKEVKEVKRARLTEYAAAKPSAQYFAKQNGEHGVWQYKVDLALPC
ATQNELDIEDAKMLVANGVVSVTEGANMPTTLEATKYLQENGVLFVGGKAANAGGVATSALEMSQNSERLSWTFEEVDGK
LKGIMETIYANISDAAKRYDMTVGGKTDYVAGANIAGFEKVVDAMLAQGVC

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI4885281, Length=404, Percent_Identity=28.960396039604, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI31377775, Length=403, Percent_Identity=29.0322580645161, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1788059, Length=451, Percent_Identity=56.7627494456763, Blast_Score=505, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17544676, Length=391, Percent_Identity=28.3887468030691, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324951, Length=458, Percent_Identity=50, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319256, Length=452, Percent_Identity=48.6725663716814, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI45549226, Length=380, Percent_Identity=30.2631578947368, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45553475, Length=380, Percent_Identity=30.2631578947368, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24649283, Length=375, Percent_Identity=30.4, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24649460, Length=296, Percent_Identity=32.4324324324324, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48972; Mature: 48841

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYVDEVLDIVSKKNADQPEFLQAVTEVLESLRPVVEANEEVYRKNAILERITEPDRQIM
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
FRVPWVDDKGQVQVNRGFRVQFNNAIGPYKGGLRLHPSVNLGIIKFLGFEQVFKNSLTTL
EEECCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHCHHHHHHCCCCEE
PIGGGKGGSDFDPKGKSDREIMAFCQSFMTELCKYIGADVDVPAGDIGTGAREIGFMFGQ
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
YKRIRGMFEGVLTGKGLTYGGSLARTEATGYGLLYLTNALWKDNGMSLEGKTAAVSGSGN
HHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEHHHCCCCCCCCCCCEEEEECCCC
VAIYAIQKAQQLGVKVVTCSDSTGWIYDPEGIDVALLKEVKEVKRARLTEYAAAKPSAQY
EEEEEEEEHHHCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
FAKQNGEHGVWQYKVDLALPCATQNELDIEDAKMLVANGVVSVTEGANMPTTLEATKYLQ
HHHCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHH
ENGVLFVGGKAANAGGVATSALEMSQNSERLSWTFEEVDGKLKGIMETIYANISDAAKRY
HCCEEEEECCCCCCCCHHHHHHHHCCCCCHHEEEHHHHCHHHHHHHHHHHHCHHHHHHHH
DMTVGGKTDYVAGANIAGFEKVVDAMLAQGVC
CEEECCCCCEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GYVDEVLDIVSKKNADQPEFLQAVTEVLESLRPVVEANEEVYRKNAILERITEPDRQIM
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
FRVPWVDDKGQVQVNRGFRVQFNNAIGPYKGGLRLHPSVNLGIIKFLGFEQVFKNSLTTL
EEECCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHCHHHHHHCCCCEE
PIGGGKGGSDFDPKGKSDREIMAFCQSFMTELCKYIGADVDVPAGDIGTGAREIGFMFGQ
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
YKRIRGMFEGVLTGKGLTYGGSLARTEATGYGLLYLTNALWKDNGMSLEGKTAAVSGSGN
HHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEHHHCCCCCCCCCCCEEEEECCCC
VAIYAIQKAQQLGVKVVTCSDSTGWIYDPEGIDVALLKEVKEVKRARLTEYAAAKPSAQY
EEEEEEEEHHHCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
FAKQNGEHGVWQYKVDLALPCATQNELDIEDAKMLVANGVVSVTEGANMPTTLEATKYLQ
HHHCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHH
ENGVLFVGGKAANAGGVATSALEMSQNSERLSWTFEEVDGKLKGIMETIYANISDAAKRY
HCCEEEEECCCCCCCCHHHHHHHHCCCCCHHEEEHHHHCHHHHHHHHHHHHCHHHHHHHH
DMTVGGKTDYVAGANIAGFEKVVDAMLAQGVC
CEEECCCCCEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]