Click here to switch to the map view.

The map label for this gene is folC [H]

Identifier: 238917533

GI number: 238917533

Start: 1664745

End: 1666028

Strand: Reverse

Name: folC [H]

Synonym: EUBELI_01612

Alternate gene names: 238917533

Gene position: 1666028-1664745 (Counterclockwise)

Preceding gene: 238917534

Following gene: 238917532

Centisome position: 77.7

GC content: 36.14

Gene sequence:

>1284_bases
ATGTATGATGAAATTATAGACAGACTTAACAGTATAGGACAGTTCGCTTCATCTTCAGGTGTACAGCGCGCAGAAATACT
GTTGAGGGATATGAATAATCCGGAAGAAAAGCTTAATATCATACATGTTGCAGGAACTAACGGAAAGGGATCTGTTTGTG
CATATATAGCGCAGATTCTTCAGGAAAACGGTTATAAGACAGGCCTTTTTACATCTCCTCATCTTGTAGATGTCCGGGAG
AGAATACGAATTAACAATGAAATGATTTCTAAAGAAGATTTTGTAGAAGCATATGAATATGTTGAAGAACATCATAGTGG
TGAACTGGCATATTTTGATTATCTTTTTGGAATGGCAGTATATTATTATAACAGGCAGAATGTTGATTATGTTGTTATGG
AAACAGGACTTGGAGGAAGACTGGATGCCACTAATGCAGTAAAACAGCCACTTATATCTGTTATATCAACAATATCACTT
GAACATACTGCAATATTAGGAGATACAATAGAAAAGATTGCAACAGAGAAAGCAGGAATAATAAAGCCTGGAATACCGGT
AGTGTGCAGCGGTGTGGACAAGCATGCTGCAAATGTAATAAAAGAAATTGCATCTGATAATTCAAGTGCATGCATTGTTT
TGGATGAGAATTCTTATGAACTTAAGCAAAATACATATGGTAATATTGATTTTTTAATTCATAATGAGTATTATAAGAAT
GATTGCTTCAGACTGGCTACTAATGCCTTATACCAGATTGAGAATGCAAGTATTGCACTTACTGTATGTGCAGTTCTTAA
AGATTCAGGTGTGAAACTGGATGACAGACTAACAAAAGAAGCTGTCAGCAAAACTCACTGGGCAGGAAGAATGGAACCGG
TTAAGGAGAATTTGTATGTTGATGGGGCACATAATCCGCAGGGGATAAGGAGCTTTGTGGATTCTGTGAACAGCCTTTAT
GAAAGCGAAGAATTATCTGATGCCACATTGCTTTTTTCAGTGGTAAGTGATAAGAATTATGAACAGATGATAAAGATATT
GTGTGAATGTAAACACTTCAAACATATATATGTTACGATGACAGGAGGTGCGAGAAGGCTTTCTTCGGAGATTATAAAAC
AGACATTTGAAGTGTATTTATCGGGGATTTCTATAGAAATATCTGACAGTGTGGACAGAGTCATACATGAATGGGATAAT
GGACTTATGTTTGCAACAGGTTCATTGTATCTTGTGGGTGATGTTGTCCGTGCATTAGAAAGAAAGGAAAATAATCATGA
TTAA

Upstream 100 bases:

>100_bases
GAGCAGATAAAAGCATATACAGGTGGCAGAAGGATAGCAGTGATAAGCTATAAAGAACATAGAATTACTGCCATAGACTG
CACATAGGAAGGATGAAACA

Downstream 100 bases:

>100_bases
TTTTGATGAAGAGATACAGAAGTTTCAGCCAAGTCTTGAAGTAGAAGACGCAGAGGATGCAATATATAACAATGATGTTC
CGGATATTACGGATTTGATA

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MYDEIIDRLNSIGQFASSSGVQRAEILLRDMNNPEEKLNIIHVAGTNGKGSVCAYIAQILQENGYKTGLFTSPHLVDVRE
RIRINNEMISKEDFVEAYEYVEEHHSGELAYFDYLFGMAVYYYNRQNVDYVVMETGLGGRLDATNAVKQPLISVISTISL
EHTAILGDTIEKIATEKAGIIKPGIPVVCSGVDKHAANVIKEIASDNSSACIVLDENSYELKQNTYGNIDFLIHNEYYKN
DCFRLATNALYQIENASIALTVCAVLKDSGVKLDDRLTKEAVSKTHWAGRMEPVKENLYVDGAHNPQGIRSFVDSVNSLY
ESEELSDATLLFSVVSDKNYEQMIKILCECKHFKHIYVTMTGGARRLSSEIIKQTFEVYLSGISIEISDSVDRVIHEWDN
GLMFATGSLYLVGDVVRALERKENNHD

Sequences:

>Translated_427_residues
MYDEIIDRLNSIGQFASSSGVQRAEILLRDMNNPEEKLNIIHVAGTNGKGSVCAYIAQILQENGYKTGLFTSPHLVDVRE
RIRINNEMISKEDFVEAYEYVEEHHSGELAYFDYLFGMAVYYYNRQNVDYVVMETGLGGRLDATNAVKQPLISVISTISL
EHTAILGDTIEKIATEKAGIIKPGIPVVCSGVDKHAANVIKEIASDNSSACIVLDENSYELKQNTYGNIDFLIHNEYYKN
DCFRLATNALYQIENASIALTVCAVLKDSGVKLDDRLTKEAVSKTHWAGRMEPVKENLYVDGAHNPQGIRSFVDSVNSLY
ESEELSDATLLFSVVSDKNYEQMIKILCECKHFKHIYVTMTGGARRLSSEIIKQTFEVYLSGISIEISDSVDRVIHEWDN
GLMFATGSLYLVGDVVRALERKENNHD
>Mature_427_residues
MYDEIIDRLNSIGQFASSSGVQRAEILLRDMNNPEEKLNIIHVAGTNGKGSVCAYIAQILQENGYKTGLFTSPHLVDVRE
RIRINNEMISKEDFVEAYEYVEEHHSGELAYFDYLFGMAVYYYNRQNVDYVVMETGLGGRLDATNAVKQPLISVISTISL
EHTAILGDTIEKIATEKAGIIKPGIPVVCSGVDKHAANVIKEIASDNSSACIVLDENSYELKQNTYGNIDFLIHNEYYKN
DCFRLATNALYQIENASIALTVCAVLKDSGVKLDDRLTKEAVSKTHWAGRMEPVKENLYVDGAHNPQGIRSFVDSVNSLY
ESEELSDATLLFSVVSDKNYEQMIKILCECKHFKHIYVTMTGGARRLSSEIIKQTFEVYLSGISIEISDSVDRVIHEWDN
GLMFATGSLYLVGDVVRALERKENNHD

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=341, Percent_Identity=30.791788856305, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI66932990, Length=344, Percent_Identity=31.1046511627907, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1788654, Length=303, Percent_Identity=28.0528052805281, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=397, Percent_Identity=27.455919395466, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17553148, Length=397, Percent_Identity=27.455919395466, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17553150, Length=397, Percent_Identity=27.455919395466, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6323760, Length=351, Percent_Identity=33.3333333333333, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324815, Length=330, Percent_Identity=29.0909090909091, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322718, Length=258, Percent_Identity=25.968992248062, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24641571, Length=322, Percent_Identity=30.4347826086957, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24581568, Length=149, Percent_Identity=34.2281879194631, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47860; Mature: 47860

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDEIIDRLNSIGQFASSSGVQRAEILLRDMNNPEEKLNIIHVAGTNGKGSVCAYIAQIL
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHEEEEEEECCCCCCHHHHHHHHHH
QENGYKTGLFTSPHLVDVRERIRINNEMISKEDFVEAYEYVEEHHSGELAYFDYLFGMAV
HHCCCCCCCCCCCCHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YYYNRQNVDYVVMETGLGGRLDATNAVKQPLISVISTISLEHTAILGDTIEKIATEKAGI
HEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IKPGIPVVCSGVDKHAANVIKEIASDNSSACIVLDENSYELKQNTYGNIDFLIHNEYYKN
CCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCCEEEEEECCHHHH
DCFRLATNALYQIENASIALTVCAVLKDSGVKLDDRLTKEAVSKTHWAGRMEPVKENLYV
HHHHHHHHHHHEECCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCEEE
DGAHNPQGIRSFVDSVNSLYESEELSDATLLFSVVSDKNYEQMIKILCECKHFKHIYVTM
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEE
TGGARRLSSEIIKQTFEVYLSGISIEISDSVDRVIHEWDNGLMFATGSLYLVGDVVRALE
CCCHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
RKENNHD
HHCCCCC
>Mature Secondary Structure
MYDEIIDRLNSIGQFASSSGVQRAEILLRDMNNPEEKLNIIHVAGTNGKGSVCAYIAQIL
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHEEEEEEECCCCCCHHHHHHHHHH
QENGYKTGLFTSPHLVDVRERIRINNEMISKEDFVEAYEYVEEHHSGELAYFDYLFGMAV
HHCCCCCCCCCCCCHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YYYNRQNVDYVVMETGLGGRLDATNAVKQPLISVISTISLEHTAILGDTIEKIATEKAGI
HEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IKPGIPVVCSGVDKHAANVIKEIASDNSSACIVLDENSYELKQNTYGNIDFLIHNEYYKN
CCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCCEEEEEECCHHHH
DCFRLATNALYQIENASIALTVCAVLKDSGVKLDDRLTKEAVSKTHWAGRMEPVKENLYV
HHHHHHHHHHHEECCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCEEE
DGAHNPQGIRSFVDSVNSLYESEELSDATLLFSVVSDKNYEQMIKILCECKHFKHIYVTM
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEE
TGGARRLSSEIIKQTFEVYLSGISIEISDSVDRVIHEWDNGLMFATGSLYLVGDVVRALE
CCCHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
RKENNHD
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]