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The map label for this gene is pgl [H]

Identifier: 238917515

GI number: 238917515

Start: 1646848

End: 1648404

Strand: Reverse

Name: pgl [H]

Synonym: EUBELI_01594

Alternate gene names: 238917515

Gene position: 1648404-1646848 (Counterclockwise)

Preceding gene: 238917516

Following gene: 238917514

Centisome position: 76.88

GC content: 36.93

Gene sequence:

>1557_bases
ATGGAGTTAAAGTTAGTTATCAAAACAGGAAGAAGTGCTGTAGTAGAATTTGATGACGGTGGAAAGTATTATTCAAAGGA
AGAATACACACTCCTGGTTAATGGAGAAGAATATGGCAAGACAGAAAAGGTTGTTACAACAATATATGGTCTTAAGCCAG
ATACAGAATATAAGATTTCAGCTTTGTATGCAGGAAAAGAATATGGACCTGTTGAATTTAAGACAGATTATGAATATGTA
ACACTTAATGTCAGAGAATTTGGTGCGTATGGTGATGGAGAACATGATGATACTAATGCAATCCAGTGTGCGATTATGGC
AGCTCCTAAGGATTCCAGAGTATTAGTTCCAGAGGGTGTTTATAAGATATCAAGCATATTTTTAAAAGATAATCTTAACC
TTGAGCTTGCTAAAGGAGCAGTACTTTCAGCATTTACAGAAAGAGAAAAGTTCCCTATATTACCGGGACAGATTGAGACA
TACGATGAGAAAGATTATTACAATCTTGGTACATGGGAAGGCAATCCTCTGGATATGTTTTCAGCAATTGTATGTGGTAT
TAACTGTTCAAATGTTACAATTACAGGTGAAGGAACTATTGATGGATGTACAACTCACGATAACTGGTGGAAGAATTGTA
AGATAAGAAATACTGCGTGGAGACCAAGATTATTCTTTATCAATAACTGTAGTAATGTTACTATGCATGGAATTACAGTT
CAGAATTCACCATCATGGACAATCCACCCATATTTTAGTAAACATCTTAAGTTTATTGATGTTAAGATTCTTAATCCTGC
TAATTCACACAACACAGATGGTCTTGACCCGGAAAGCTGTCAGGATGTTCTTGTACTTGGAACATACATATCAGTTGGCG
ATGACTGTATAGCAATCAAGTCAGGCAAGATATATATGGCTCAGAAGGAAAAGACTCCTACAGAGGATATGACAGTACGC
CAGTGCTGTATGAGAGACGGACACGGTGCAGTAACAGTAGGAAGCGAGATTGCAGCTGGAGTTAAAGATGTTCATATCAG
AGACTGTATGTTCATGAATACAGACAGAGGTTTAAGAGTTAAGACAAGAAGAGGTCGTGGTAAATTATCTGTACTTGATG
ATATTTCGTTTGAGAATATTGATATGGATAATGTTATGACACCATTTGTTGTTAACAGTTTCTATTTCTGTGATCCGGAT
GGAAAGACAGAGTATGTTGGCTCTAGAAAGCCGCTTCCTGTTGATGACAGAACACCTTCAATTAAGAGTCTTACATTTAA
GGATATCAATGCGAAAAACTGCCATGTTGCAGGTGCATATATATTCGGTCTGCCAGAGAGTAAAGTTGAGAAGCTTACAT
TTGAGAATATTAACTTCAGCTATGCTAAAGATGCAAAGCCGGGTGTAGCAGCAATGATGCTTGGCTGTGATGAAGCAAGC
AGACAGGGACTTATTGTAAGTAATGTAGAAAAACTTATATTAAAGAATGTCAATATTGAAGGCTGTGATGGAGAAGCAAT
TGTGGCAGATAATGTTGATAAGATTGAAAGAGATTAG

Upstream 100 bases:

>100_bases
AGAGCAGTGTAGCTGTATCAGCAGCATACATAACAGCTTACAGTGAGTATATAAGAAACAGGGATTATCAGGATTACGGA
CGCAGCAATGGAGGTGTATT

Downstream 100 bases:

>100_bases
TATAAATATATAGAAATATGAGGAACAATCATGAATTTACATGCACCAAAGGGAATTGATGTTGAGAATTGGTTCATTGA
GTGCTATAGGAAACATAAGG

Product: glycoside hydrolase family 28

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MELKLVIKTGRSAVVEFDDGGKYYSKEEYTLLVNGEEYGKTEKVVTTIYGLKPDTEYKISALYAGKEYGPVEFKTDYEYV
TLNVREFGAYGDGEHDDTNAIQCAIMAAPKDSRVLVPEGVYKISSIFLKDNLNLELAKGAVLSAFTEREKFPILPGQIET
YDEKDYYNLGTWEGNPLDMFSAIVCGINCSNVTITGEGTIDGCTTHDNWWKNCKIRNTAWRPRLFFINNCSNVTMHGITV
QNSPSWTIHPYFSKHLKFIDVKILNPANSHNTDGLDPESCQDVLVLGTYISVGDDCIAIKSGKIYMAQKEKTPTEDMTVR
QCCMRDGHGAVTVGSEIAAGVKDVHIRDCMFMNTDRGLRVKTRRGRGKLSVLDDISFENIDMDNVMTPFVVNSFYFCDPD
GKTEYVGSRKPLPVDDRTPSIKSLTFKDINAKNCHVAGAYIFGLPESKVEKLTFENINFSYAKDAKPGVAAMMLGCDEAS
RQGLIVSNVEKLILKNVNIEGCDGEAIVADNVDKIERD

Sequences:

>Translated_518_residues
MELKLVIKTGRSAVVEFDDGGKYYSKEEYTLLVNGEEYGKTEKVVTTIYGLKPDTEYKISALYAGKEYGPVEFKTDYEYV
TLNVREFGAYGDGEHDDTNAIQCAIMAAPKDSRVLVPEGVYKISSIFLKDNLNLELAKGAVLSAFTEREKFPILPGQIET
YDEKDYYNLGTWEGNPLDMFSAIVCGINCSNVTITGEGTIDGCTTHDNWWKNCKIRNTAWRPRLFFINNCSNVTMHGITV
QNSPSWTIHPYFSKHLKFIDVKILNPANSHNTDGLDPESCQDVLVLGTYISVGDDCIAIKSGKIYMAQKEKTPTEDMTVR
QCCMRDGHGAVTVGSEIAAGVKDVHIRDCMFMNTDRGLRVKTRRGRGKLSVLDDISFENIDMDNVMTPFVVNSFYFCDPD
GKTEYVGSRKPLPVDDRTPSIKSLTFKDINAKNCHVAGAYIFGLPESKVEKLTFENINFSYAKDAKPGVAAMMLGCDEAS
RQGLIVSNVEKLILKNVNIEGCDGEAIVADNVDKIERD
>Mature_518_residues
MELKLVIKTGRSAVVEFDDGGKYYSKEEYTLLVNGEEYGKTEKVVTTIYGLKPDTEYKISALYAGKEYGPVEFKTDYEYV
TLNVREFGAYGDGEHDDTNAIQCAIMAAPKDSRVLVPEGVYKISSIFLKDNLNLELAKGAVLSAFTEREKFPILPGQIET
YDEKDYYNLGTWEGNPLDMFSAIVCGINCSNVTITGEGTIDGCTTHDNWWKNCKIRNTAWRPRLFFINNCSNVTMHGITV
QNSPSWTIHPYFSKHLKFIDVKILNPANSHNTDGLDPESCQDVLVLGTYISVGDDCIAIKSGKIYMAQKEKTPTEDMTVR
QCCMRDGHGAVTVGSEIAAGVKDVHIRDCMFMNTDRGLRVKTRRGRGKLSVLDDISFENIDMDNVMTPFVVNSFYFCDPD
GKTEYVGSRKPLPVDDRTPSIKSLTFKDINAKNCHVAGAYIFGLPESKVEKLTFENINFSYAKDAKPGVAAMMLGCDEAS
RQGLIVSNVEKLILKNVNIEGCDGEAIVADNVDKIERD

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 57712; Mature: 57712

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKLVIKTGRSAVVEFDDGGKYYSKEEYTLLVNGEEYGKTEKVVTTIYGLKPDTEYKIS
CEEEEEEECCCEEEEEECCCCCEECCCCEEEEECCHHHCCCCEEEEEEECCCCCCCEEEE
ALYAGKEYGPVEFKTDYEYVTLNVREFGAYGDGEHDDTNAIQCAIMAAPKDSRVLVPEGV
EEEECCCCCCEEEECCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEECCCCC
YKISSIFLKDNLNLELAKGAVLSAFTEREKFPILPGQIETYDEKDYYNLGTWEGNPLDMF
EEEEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHH
SAIVCGINCSNVTITGEGTIDGCTTHDNWWKNCKIRNTAWRPRLFFINNCSNVTMHGITV
HHHHHCCCCCEEEEECCCCCCCCCCCCCHHCCCEEECCCCCCEEEEEECCCCEEEEEEEE
QNSPSWTIHPYFSKHLKFIDVKILNPANSHNTDGLDPESCQDVLVLGTYISVGDDCIAIK
ECCCCEEECCCHHCCEEEEEEEEECCCCCCCCCCCCHHHCCCEEEEEEEEECCCCEEEEE
SGKIYMAQKEKTPTEDMTVRQCCMRDGHGAVTVGSEIAAGVKDVHIRDCMFMNTDRGLRV
CCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECHHHHCCCCEEEEEEEEEEECCCCEEE
KTRRGRGKLSVLDDISFENIDMDNVMTPFVVNSFYFCDPDGKTEYVGSRKPLPVDDRTPS
EEECCCCCEEEEECCCCCCCCHHHCCCCHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCC
IKSLTFKDINAKNCHVAGAYIFGLPESKVEKLTFENINFSYAKDAKPGVAAMMLGCDEAS
EEEEEEECCCCCCEEEEEEEEEECCHHHHHEEEEECCCEEECCCCCCCEEEEEEECCHHH
RQGLIVSNVEKLILKNVNIEGCDGEAIVADNVDKIERD
CCCEEHHHHHHHHHHCCCCCCCCCCEEEECCCHHHCCC
>Mature Secondary Structure
MELKLVIKTGRSAVVEFDDGGKYYSKEEYTLLVNGEEYGKTEKVVTTIYGLKPDTEYKIS
CEEEEEEECCCEEEEEECCCCCEECCCCEEEEECCHHHCCCCEEEEEEECCCCCCCEEEE
ALYAGKEYGPVEFKTDYEYVTLNVREFGAYGDGEHDDTNAIQCAIMAAPKDSRVLVPEGV
EEEECCCCCCEEEECCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEECCCCC
YKISSIFLKDNLNLELAKGAVLSAFTEREKFPILPGQIETYDEKDYYNLGTWEGNPLDMF
EEEEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHH
SAIVCGINCSNVTITGEGTIDGCTTHDNWWKNCKIRNTAWRPRLFFINNCSNVTMHGITV
HHHHHCCCCCEEEEECCCCCCCCCCCCCHHCCCEEECCCCCCEEEEEECCCCEEEEEEEE
QNSPSWTIHPYFSKHLKFIDVKILNPANSHNTDGLDPESCQDVLVLGTYISVGDDCIAIK
ECCCCEEECCCHHCCEEEEEEEEECCCCCCCCCCCCHHHCCCEEEEEEEEECCCCEEEEE
SGKIYMAQKEKTPTEDMTVRQCCMRDGHGAVTVGSEIAAGVKDVHIRDCMFMNTDRGLRV
CCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECHHHHCCCCEEEEEEEEEEECCCCEEE
KTRRGRGKLSVLDDISFENIDMDNVMTPFVVNSFYFCDPDGKTEYVGSRKPLPVDDRTPS
EEECCCCCEEEEECCCCCCCCHHHCCCCHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCC
IKSLTFKDINAKNCHVAGAYIFGLPESKVEKLTFENINFSYAKDAKPGVAAMMLGCDEAS
EEEEEEECCCCCCEEEEEEEEEECCHHHHHEEEEECCCEEECCCCCCCEEEEEEECCHHH
RQGLIVSNVEKLILKNVNIEGCDGEAIVADNVDKIERD
CCCEEHHHHHHHHHHCCCCCCCCCCEEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]