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The map label for this gene is sleB [H]

Identifier: 238917486

GI number: 238917486

Start: 1616917

End: 1618059

Strand: Direct

Name: sleB [H]

Synonym: EUBELI_01564

Alternate gene names: 238917486

Gene position: 1616917-1618059 (Clockwise)

Preceding gene: 238917483

Following gene: 238917487

Centisome position: 75.41

GC content: 43.66

Gene sequence:

>1143_bases
ATGGCAGCTACATTTGCTGTTCCATTTTTTGCTTATCCTGACCAGTTGATGGCTGATACACAGAATGACCTCGACGCTGT
TAATAAACAGCTTGAAGAATTAAGAAATAAACAGAGCGAACTCAATGCATCTTACGGGGAGCTTAACGAGAAGCTGTCTG
CTTCCGGAGAGAAGCTGTCTTCTATTGAGGATGCAGTTAATGCGAAGCAGTCTGAGATTGATGCTACCAATATCCAGGTT
GCTGATATGCAGGCAGAGATTGACCAACAGTACGCCGCCATGAAACTTCGTATTCAGTTTATGTATGAGAATAATAATAC
TACTATCCTGTCAACGCTTCTGTCGGCTGAAAGCCTTTCTGACCTTTTATCCAAGTCAGAATATATCCAGCAGATTTCTA
ATTATGACCACCAGAAGATGCAGGAATTAAGTAACCTTCTGGCTTCTCTTAAAGAAACTCAGGCAAAGCTTGAAGAAGAG
ATGACCGAACTTGTCACTCTTAAAGATGATGCAGCCCGCGAAGCTGATAATTTTGCTGTGCTTCTCTCACAATGCCAGAC
AGAGCTTGACACTACAAGTGACAGCATTACTGATGCAGAGGCACTTGCGCTTGAATACGAAAAGCAGATTGAACAGGAAA
TGTTAGAGCGTCAGCGCCGTGAAATGGAAGCCCTTGAAGCTGCAAAAAAAGCACAGGAAGAGGCTGACAAAGTCAATAAC
GCCGGTAATACAGGCAATAATTCATCTTCCGGCTCTGCTATGGTTGACCAGAACGCTCTTAACAATGTTCTTAAGAATCA
CACTGCCGAAGATGTTGCAATGCTCGCAGCAATTATTGAATGTGAAGCCGGCAACCAGTCATATGAGGGCAAATGTGCTG
TAGGAAGTGTTGTTATCAACAGAGTTGCAGACCCACGCTTTGCCAACAGCATAAGCGGCGTAATATATGCACCTTACCAG
TTTTCACCTGTTGCCAGCGGACGATTTGCAATAGTACTTGCAAGAGGTGCAAATGCAGCCTGCACCCAGGCAGCCGTTGA
TGTGCTTAACGGTTATATCAATATTAACGCACTATATTTTCATGTATATGACAGTTCTGTCGATGTGGGCGGAACCGTTA
TCGGAGATCATGTATTTTACTAA

Upstream 100 bases:

>100_bases
TTTTTTGTTTATAAAAATATAATGATTACTAGGAGTCTATGTGAAACATTTTATCAGATTAAGATTAAAGAATTTAACAG
CCATTGGTCTTTCGCTTTGC

Downstream 100 bases:

>100_bases
CTCATTAAATATACCAAGGGGAGAATTATTAACTATGAAGAAAGCAACTAAAGCATCTGATAACAGATATTATCAAGCAA
GATTTTCTGCTGCACAGAAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: SCLE [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MAATFAVPFFAYPDQLMADTQNDLDAVNKQLEELRNKQSELNASYGELNEKLSASGEKLSSIEDAVNAKQSEIDATNIQV
ADMQAEIDQQYAAMKLRIQFMYENNNTTILSTLLSAESLSDLLSKSEYIQQISNYDHQKMQELSNLLASLKETQAKLEEE
MTELVTLKDDAAREADNFAVLLSQCQTELDTTSDSITDAEALALEYEKQIEQEMLERQRREMEALEAAKKAQEEADKVNN
AGNTGNNSSSGSAMVDQNALNNVLKNHTAEDVAMLAAIIECEAGNQSYEGKCAVGSVVINRVADPRFANSISGVIYAPYQ
FSPVASGRFAIVLARGANAACTQAAVDVLNGYININALYFHVYDSSVDVGGTVIGDHVFY

Sequences:

>Translated_380_residues
MAATFAVPFFAYPDQLMADTQNDLDAVNKQLEELRNKQSELNASYGELNEKLSASGEKLSSIEDAVNAKQSEIDATNIQV
ADMQAEIDQQYAAMKLRIQFMYENNNTTILSTLLSAESLSDLLSKSEYIQQISNYDHQKMQELSNLLASLKETQAKLEEE
MTELVTLKDDAAREADNFAVLLSQCQTELDTTSDSITDAEALALEYEKQIEQEMLERQRREMEALEAAKKAQEEADKVNN
AGNTGNNSSSGSAMVDQNALNNVLKNHTAEDVAMLAAIIECEAGNQSYEGKCAVGSVVINRVADPRFANSISGVIYAPYQ
FSPVASGRFAIVLARGANAACTQAAVDVLNGYININALYFHVYDSSVDVGGTVIGDHVFY
>Mature_379_residues
AATFAVPFFAYPDQLMADTQNDLDAVNKQLEELRNKQSELNASYGELNEKLSASGEKLSSIEDAVNAKQSEIDATNIQVA
DMQAEIDQQYAAMKLRIQFMYENNNTTILSTLLSAESLSDLLSKSEYIQQISNYDHQKMQELSNLLASLKETQAKLEEEM
TELVTLKDDAAREADNFAVLLSQCQTELDTTSDSITDAEALALEYEKQIEQEMLERQRREMEALEAAKKAQEEADKVNNA
GNTGNNSSSGSAMVDQNALNNVLKNHTAEDVAMLAAIIECEAGNQSYEGKCAVGSVVINRVADPRFANSISGVIYAPYQF
SPVASGRFAIVLARGANAACTQAAVDVLNGYININALYFHVYDSSVDVGGTVIGDHVFY

Specific function: Could be a lytic transglycosylase. Required for spore cortex hydrolysis during germination. Interacts strongly but noncovalently with spore components [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 41587; Mature: 41456

Theoretical pI: Translated: 4.07; Mature: 4.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATFAVPFFAYPDQLMADTQNDLDAVNKQLEELRNKQSELNASYGELNEKLSASGEKLS
CCCEEECCHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
SIEDAVNAKQSEIDATNIQVADMQAEIDQQYAAMKLRIQFMYENNNTTILSTLLSAESLS
HHHHHHCCHHHCCCCCCEEEEHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHH
DLLSKSEYIQQISNYDHQKMQELSNLLASLKETQAKLEEEMTELVTLKDDAAREADNFAV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LLSQCQTELDTTSDSITDAEALALEYEKQIEQEMLERQRREMEALEAAKKAQEEADKVNN
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AGNTGNNSSSGSAMVDQNALNNVLKNHTAEDVAMLAAIIECEAGNQSYEGKCAVGSVVIN
CCCCCCCCCCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RVADPRFANSISGVIYAPYQFSPVASGRFAIVLARGANAACTQAAVDVLNGYININALYF
HHCCCHHHHCCCCEEECCEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCEEEEEEEEE
HVYDSSVDVGGTVIGDHVFY
EEECCCCCCCCEECCCCCCC
>Mature Secondary Structure 
AATFAVPFFAYPDQLMADTQNDLDAVNKQLEELRNKQSELNASYGELNEKLSASGEKLS
CCEEECCHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
SIEDAVNAKQSEIDATNIQVADMQAEIDQQYAAMKLRIQFMYENNNTTILSTLLSAESLS
HHHHHHCCHHHCCCCCCEEEEHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHH
DLLSKSEYIQQISNYDHQKMQELSNLLASLKETQAKLEEEMTELVTLKDDAAREADNFAV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LLSQCQTELDTTSDSITDAEALALEYEKQIEQEMLERQRREMEALEAAKKAQEEADKVNN
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AGNTGNNSSSGSAMVDQNALNNVLKNHTAEDVAMLAAIIECEAGNQSYEGKCAVGSVVIN
CCCCCCCCCCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RVADPRFANSISGVIYAPYQFSPVASGRFAIVLARGANAACTQAAVDVLNGYININALYF
HHCCCHHHHCCCCEEECCEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCEEEEEEEEE
HVYDSSVDVGGTVIGDHVFY
EEECCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8830707; 8760912; 9384377; 10197998; 10658652 [H]