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The map label for this gene is sigG [H]

Identifier: 238917256

GI number: 238917256

Start: 1364556

End: 1365332

Strand: Direct

Name: sigG [H]

Synonym: EUBELI_01330

Alternate gene names: 238917256

Gene position: 1364556-1365332 (Clockwise)

Preceding gene: 238917249

Following gene: 238917257

Centisome position: 63.64

GC content: 39.38

Gene sequence:

>777_bases
ATGGCTGGATACAAAGTCGAAATATGCGGAGTCAATACAAGTGACCTTCCTCTTCTTAAACCCGCCCAGGCAAAAGAACT
GCTTGTAAAGATAAAATCAGGAGATACGCAGGCAAGAGAAACATTTATAAAAGGAAACTTAAGGCTTGTATTAAGCATTG
TACAACGCTTCAGCCAGAGTGGTGAAAATCTTGATGACCTGTTTCAGATAGGCTGTATAGGAATGATTAAAGCGATTGAC
AATTTTGATATATCGTTAAATGTACAGTTTTCAACATATGCTGTTCCCATGATAATCGGTGAGATAAAGCGTTTCCTGCG
TGATAACAGCACTATAAGAGTCAGCCGTTCCTTAAAAGATACAGCCTATAAAGCAATAACTACCCGTGAAACACTTATGA
AGAAAAATCTTAAAGAACCATCAATTACAGAAATAGCAGAAGAAATAGGCATTTCAAAAGCTGATATCGTAATTGCCCTT
GAAGCAGTCACACCTTCAATATCATTATATGAACCGGTATTCAATGATGGTGGTGACACCCTGTATGTTATTGATCAGGT
AAAGGACAAGAAAAACACTGAAGACCGATGGGTAAGAAATATTGCACTGCAAGAAGCAATAAACCGGCTTTCCCCAAGAG
AACGCCAGATAATAATACTCCGTTTCTTTGATTGTCTCACACAGACTGAAGTTGCCAAAGGCCTTGGGATATCACAGGCC
CAGGTAAGCCGTCTTGAAAAATGTGCCTTGCGTGATATGAAAAGACACCTTTGCTGA

Upstream 100 bases:

>100_bases
CAAATTAAATATATAAATTAATTATGAATAAGCCCAGCCAAAATGTCAATCCTTTTAATGTTGATAAATTGAAAAACCAA
TTATTATTTAAGGAGGGATT

Downstream 100 bases:

>100_bases
TATTCGTAATATAATAATATAAGCACTTAACCAGGTATTATAATGCGTCTATGTGAACTTAAAGGAAAAACTGTAATAAA
TGATACCGACTGCTGCATAT

Product: RNA polymerase sporulation-specific sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAGYKVEICGVNTSDLPLLKPAQAKELLVKIKSGDTQARETFIKGNLRLVLSIVQRFSQSGENLDDLFQIGCIGMIKAID
NFDISLNVQFSTYAVPMIIGEIKRFLRDNSTIRVSRSLKDTAYKAITTRETLMKKNLKEPSITEIAEEIGISKADIVIAL
EAVTPSISLYEPVFNDGGDTLYVIDQVKDKKNTEDRWVRNIALQEAINRLSPRERQIIILRFFDCLTQTEVAKGLGISQA
QVSRLEKCALRDMKRHLC

Sequences:

>Translated_258_residues
MAGYKVEICGVNTSDLPLLKPAQAKELLVKIKSGDTQARETFIKGNLRLVLSIVQRFSQSGENLDDLFQIGCIGMIKAID
NFDISLNVQFSTYAVPMIIGEIKRFLRDNSTIRVSRSLKDTAYKAITTRETLMKKNLKEPSITEIAEEIGISKADIVIAL
EAVTPSISLYEPVFNDGGDTLYVIDQVKDKKNTEDRWVRNIALQEAINRLSPRERQIIILRFFDCLTQTEVAKGLGISQA
QVSRLEKCALRDMKRHLC
>Mature_257_residues
AGYKVEICGVNTSDLPLLKPAQAKELLVKIKSGDTQARETFIKGNLRLVLSIVQRFSQSGENLDDLFQIGCIGMIKAIDN
FDISLNVQFSTYAVPMIIGEIKRFLRDNSTIRVSRSLKDTAYKAITTRETLMKKNLKEPSITEIAEEIGISKADIVIALE
AVTPSISLYEPVFNDGGDTLYVIDQVKDKKNTEDRWVRNIALQEAINRLSPRERQIIILRFFDCLTQTEVAKGLGISQAQ
VSRLEKCALRDMKRHLC

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=248, Percent_Identity=28.2258064516129, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789098, Length=249, Percent_Identity=26.9076305220884, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1789871, Length=260, Percent_Identity=26.1538461538462, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788231, Length=200, Percent_Identity=28, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29025; Mature: 28894

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGYKVEICGVNTSDLPLLKPAQAKELLVKIKSGDTQARETFIKGNLRLVLSIVQRFSQS
CCCCEEEEEECCCCCCCCCCCCHHHHHEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHC
GENLDDLFQIGCIGMIKAIDNFDISLNVQFSTYAVPMIIGEIKRFLRDNSTIRVSRSLKD
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECHHHH
TAYKAITTRETLMKKNLKEPSITEIAEEIGISKADIVIALEAVTPSISLYEPVFNDGGDT
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCEEECHHHCCCCCE
LYVIDQVKDKKNTEDRWVRNIALQEAINRLSPRERQIIILRFFDCLTQTEVAKGLGISQA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHCCCCCHH
QVSRLEKCALRDMKRHLC
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGYKVEICGVNTSDLPLLKPAQAKELLVKIKSGDTQARETFIKGNLRLVLSIVQRFSQS
CCCEEEEEECCCCCCCCCCCCHHHHHEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHC
GENLDDLFQIGCIGMIKAIDNFDISLNVQFSTYAVPMIIGEIKRFLRDNSTIRVSRSLKD
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECHHHH
TAYKAITTRETLMKKNLKEPSITEIAEEIGISKADIVIALEAVTPSISLYEPVFNDGGDT
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCEEECHHHCCCCCE
LYVIDQVKDKKNTEDRWVRNIALQEAINRLSPRERQIIILRFFDCLTQTEVAKGLGISQA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHCCCCCHH
QVSRLEKCALRDMKRHLC
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961408; 7883192; 11466286 [H]