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The map label for this gene is dhaD [H]

Identifier: 238896589

GI number: 238896589

Start: 4569994

End: 4571091

Strand: Reverse

Name: dhaD [H]

Synonym: KP1_4790

Alternate gene names: 238896589

Gene position: 4571091-4569994 (Counterclockwise)

Preceding gene: 238896595

Following gene: 238896588

Centisome position: 87.09

GC content: 59.56

Gene sequence:

>1098_bases
ATGCTAAAAGTTATTCAATCTCCAGCCAAATATCTTCAGGGTCCTGATGCTGCTGTTCTGTTCGGTCAATATGCCAAAAA
CCTGGCGGAGAGCTTCTTCGTCATCGCCGACGATTTCGTAATGAAGCTGGCGGGAGAGAAAGTGGTGAATGGCCTGCAGA
GCCACGATATTCGCTGCCATGCGGAACGGTTTAACGGTGAATGCAGCCATGCGGAAATCAACCGTCTGATGGCGATTTTG
CAAAAACAGGGCTGCCGCGGCGTGGTCGGGATCGGCGGTGGTAAAACCCTCGATACCGCGAAGGCGATCGGTTACTACCA
GAAGCTGCCGGTGGTGGTGATCCCGACCATCGCCTCGACCGATGCGCCGACCAGCGCGCTGTCGGTGATCTACACCGAAG
CGGGCGAGTTTGAAGAGTATCTGATCTATCCGAAAAACCCGGATATGGTGGTGATGGACACGGCGATTATCGCCAAAGCG
CCGGTACGCCTGCTGGTCTCCGGCATGGGCGATGCGCTCTCCACCTGGTTCGAGGCCAAAGCTTGCTACGATGCGCGCGC
CACCAGCATGGCCGGAGGACAGTCCACCGAGGCGGCGCTGAGCCTCGCCCGCCTGTGCTATGATACGCTGCTGGCGGAGG
GCGAAAAGGCCCGTCTGGCGGCGCAGGCCGGGGTAGTGACCGAAGCGCTGGAGCGCATCATCGAGGCGAACACTTACCTC
AGCGGCATTGGCTTTGAAAGCAGTGGCCTGGCCGCCGCCCATGCAATCCACAACGGTTTCACCATTCTTGAAGAGTGCCA
TCACCTGTATCACGGTGAGAAAGTGGCCTTCGGTACCCTGGCGCAGCTGGTGCTGCAGAACAGCCCGATGGACGAGATTG
AAACGGTGCTGGGCTTCTGCCAGCGCGTCGGCCTGCCGGTGACGCTCGCGCAGATGGGCGTCAAAGAGGGGATCGACGAG
AAAATCGCCGCGGTGGCGAAAGCTACCTGCGCGGAAGGGGAAACCATCCATAATATGCCGTTTGCGGTGACCCCGGAGAG
CGTCCATGCCGCTATCCTCACCGCCGATCTGTTAGGCCAGCAGTGGCTGGCGCGTTAA

Upstream 100 bases:

>100_bases
CTGCTCCGGTACGCTCCGTTCAGGCCGCGCTTCACTGGCCGGCGCGGATAACGCCAGGGCTCATCATGTTTACATGCGCA
CTTATTTGAGGGTGAAAGGA

Downstream 100 bases:

>100_bases
TTCGCGGTGGCTAAACCGCTGGCCCAGGTCAGCGGTTTTTCTTTCTCCCCTCCGGCAGTCGCTGCCGGAGGGGTTCTCTA
TGGTACAACGCGGAAAAGGA

Product: putative glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MLKVIQSPAKYLQGPDAAVLFGQYAKNLAESFFVIADDFVMKLAGEKVVNGLQSHDIRCHAERFNGECSHAEINRLMAIL
QKQGCRGVVGIGGGKTLDTAKAIGYYQKLPVVVIPTIASTDAPTSALSVIYTEAGEFEEYLIYPKNPDMVVMDTAIIAKA
PVRLLVSGMGDALSTWFEAKACYDARATSMAGGQSTEAALSLARLCYDTLLAEGEKARLAAQAGVVTEALERIIEANTYL
SGIGFESSGLAAAHAIHNGFTILEECHHLYHGEKVAFGTLAQLVLQNSPMDEIETVLGFCQRVGLPVTLAQMGVKEGIDE
KIAAVAKATCAEGETIHNMPFAVTPESVHAAILTADLLGQQWLAR

Sequences:

>Translated_365_residues
MLKVIQSPAKYLQGPDAAVLFGQYAKNLAESFFVIADDFVMKLAGEKVVNGLQSHDIRCHAERFNGECSHAEINRLMAIL
QKQGCRGVVGIGGGKTLDTAKAIGYYQKLPVVVIPTIASTDAPTSALSVIYTEAGEFEEYLIYPKNPDMVVMDTAIIAKA
PVRLLVSGMGDALSTWFEAKACYDARATSMAGGQSTEAALSLARLCYDTLLAEGEKARLAAQAGVVTEALERIIEANTYL
SGIGFESSGLAAAHAIHNGFTILEECHHLYHGEKVAFGTLAQLVLQNSPMDEIETVLGFCQRVGLPVTLAQMGVKEGIDE
KIAAVAKATCAEGETIHNMPFAVTPESVHAAILTADLLGQQWLAR
>Mature_365_residues
MLKVIQSPAKYLQGPDAAVLFGQYAKNLAESFFVIADDFVMKLAGEKVVNGLQSHDIRCHAERFNGECSHAEINRLMAIL
QKQGCRGVVGIGGGKTLDTAKAIGYYQKLPVVVIPTIASTDAPTSALSVIYTEAGEFEEYLIYPKNPDMVVMDTAIIAKA
PVRLLVSGMGDALSTWFEAKACYDARATSMAGGQSTEAALSLARLCYDTLLAEGEKARLAAQAGVVTEALERIIEANTYL
SGIGFESSGLAAAHAIHNGFTILEECHHLYHGEKVAFGTLAQLVLQNSPMDEIETVLGFCQRVGLPVTLAQMGVKEGIDE
KIAAVAKATCAEGETIHNMPFAVTPESVHAAILTADLLGQQWLAR

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Exhibits a rather broad substrate specificity since it can also oxidize 1,2-prop

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=363, Percent_Identity=58.6776859504132, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1786815, Length=294, Percent_Identity=35.0340136054422, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI87082107, Length=300, Percent_Identity=26.3333333333333, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 38990; Mature: 38990

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVIQSPAKYLQGPDAAVLFGQYAKNLAESFFVIADDFVMKLAGEKVVNGLQSHDIRCH
CCCHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEE
AERFNGECSHAEINRLMAILQKQGCRGVVGIGGGKTLDTAKAIGYYQKLPVVVIPTIAST
HHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCC
DAPTSALSVIYTEAGEFEEYLIYPKNPDMVVMDTAIIAKAPVRLLVSGMGDALSTWFEAK
CCCHHHHHHHHHCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHH
ACYDARATSMAGGQSTEAALSLARLCYDTLLAEGEKARLAAQAGVVTEALERIIEANTYL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SGIGFESSGLAAAHAIHNGFTILEECHHLYHGEKVAFGTLAQLVLQNSPMDEIETVLGFC
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
QRVGLPVTLAQMGVKEGIDEKIAAVAKATCAEGETIHNMPFAVTPESVHAAILTADLLGQ
HHCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCEECCCCCEECCHHHHHHHHHHHHHHH
QWLAR
HHHCC
>Mature Secondary Structure
MLKVIQSPAKYLQGPDAAVLFGQYAKNLAESFFVIADDFVMKLAGEKVVNGLQSHDIRCH
CCCHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEE
AERFNGECSHAEINRLMAILQKQGCRGVVGIGGGKTLDTAKAIGYYQKLPVVVIPTIAST
HHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCC
DAPTSALSVIYTEAGEFEEYLIYPKNPDMVVMDTAIIAKAPVRLLVSGMGDALSTWFEAK
CCCHHHHHHHHHCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHH
ACYDARATSMAGGQSTEAALSLARLCYDTLLAEGEKARLAAQAGVVTEALERIIEANTYL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SGIGFESSGLAAAHAIHNGFTILEECHHLYHGEKVAFGTLAQLVLQNSPMDEIETVLGFC
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
QRVGLPVTLAQMGVKEGIDEKIAAVAKATCAEGETIHNMPFAVTPESVHAAILTADLLGQ
HHCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCEECCCCCEECCHHHHHHHHHHHHHHH
QWLAR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA