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The map label for this gene is glpF [H]

Identifier: 238896579

GI number: 238896579

Start: 4559672

End: 4560475

Strand: Reverse

Name: glpF [H]

Synonym: KP1_4778

Alternate gene names: 238896579

Gene position: 4560475-4559672 (Counterclockwise)

Preceding gene: 238896580

Following gene: 238896574

Centisome position: 86.89

GC content: 61.44

Gene sequence:

>804_bases
ATGAACCAGACTTCTACCTTAACCGGGCAGTGCGTGGCCGAGTTTCTTGGCACCGGATTGCTCATTTTCTTCGGCGCGGG
CTGCGTCGCTGCGCTGCGGGTCGCCGGGGCCAGCTTTGGTCAGTGGGAGATCAGTATTATCTGGGGCCTTGGCGTCGCCA
TGGCCATCTACCTGACGGCCGGTGTCTCCGGCGCGCACCTAAATCCGGCGGTGACCATTGCCCTGTGGCTGTTCGCCTGT
TTTGAACGCCGCAAGGTGCTGCCGTTTATTGTTGCCCAGACGGCCGGGGCCTTCTGCGCCGCCGCGCTGGTGTATGGGCT
CTATCGCCAGCTGTTTCTCGATCTTGAACAGAGTCAGCATATCGTGCGCGGCACTGCCGCCAGCCTTAACCTGGCCGGGG
TCTTTTCCACGTACCCGCATCCACATATCACTTTTATACAAGCGTTTGCCGTGGAGACCACCATCACGGCAATCCTGATG
GCGATGATCATGGCCCTGACCGACGACGGCAACGGAATTCCGCGCGGGCCGCTGGCGCCGTTGCTGATTGGCTTGCTGAT
CGCCGTGATCGGCGCGTCGATGGGGCCGCTAACCGGCTTTGCGCTGAATCCGGCGCGCGACTTCGGCCCAAAACTGTTTA
CCAGTCTGGCCGGGTGGGGCTCGATCGCTTTTACCGGTGGGCTGGCGATCCCTTACTTTCTGGTGCCGCTGCTGGCGCCG
GTGGTGGGGGCGATTATCGGGGCGTTTTTGTATCGCAAGCTTATCGGCCGCCATCTGCCGTGCGAATGCGGTATCGATGA
GTAA

Upstream 100 bases:

>100_bases
GCAATGCGGTCGCCACCGGGCTGCTACTGGCCGGTCAGGCGAATTAAACGGGCGCTCGCGCCAGCCTCTCTCTTTAACGT
GCTATTTCAGGATGCCGATA

Downstream 100 bases:

>100_bases
AGCGGGACCCGCCGCAGCGCGGCGGGTTTCCCCGGCCTGATGCCGGGGGGGTTATTTTTTGATGACCGCGCTGAGATCGC
CAAGTTTAGCGGCGGGGTTC

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNQTSTLTGQCVAEFLGTGLLIFFGAGCVAALRVAGASFGQWEISIIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFAC
FERRKVLPFIVAQTAGAFCAAALVYGLYRQLFLDLEQSQHIVRGTAASLNLAGVFSTYPHPHITFIQAFAVETTITAILM
AMIMALTDDGNGIPRGPLAPLLIGLLIAVIGASMGPLTGFALNPARDFGPKLFTSLAGWGSIAFTGGLAIPYFLVPLLAP
VVGAIIGAFLYRKLIGRHLPCECGIDE

Sequences:

>Translated_267_residues
MNQTSTLTGQCVAEFLGTGLLIFFGAGCVAALRVAGASFGQWEISIIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFAC
FERRKVLPFIVAQTAGAFCAAALVYGLYRQLFLDLEQSQHIVRGTAASLNLAGVFSTYPHPHITFIQAFAVETTITAILM
AMIMALTDDGNGIPRGPLAPLLIGLLIAVIGASMGPLTGFALNPARDFGPKLFTSLAGWGSIAFTGGLAIPYFLVPLLAP
VVGAIIGAFLYRKLIGRHLPCECGIDE
>Mature_267_residues
MNQTSTLTGQCVAEFLGTGLLIFFGAGCVAALRVAGASFGQWEISIIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFAC
FERRKVLPFIVAQTAGAFCAAALVYGLYRQLFLDLEQSQHIVRGTAASLNLAGVFSTYPHPHITFIQAFAVETTITAILM
AMIMALTDDGNGIPRGPLAPLLIGLLIAVIGASMGPLTGFALNPARDFGPKLFTSLAGWGSIAFTGGLAIPYFLVPLLAP
VVGAIIGAFLYRKLIGRHLPCECGIDE

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=260, Percent_Identity=42.6923076923077, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI4826645, Length=255, Percent_Identity=41.9607843137255, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI22538420, Length=258, Percent_Identity=37.5968992248062, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI4502187, Length=254, Percent_Identity=35.4330708661417, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI310133356, Length=206, Percent_Identity=32.0388349514563, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI310114181, Length=206, Percent_Identity=32.0388349514563, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI86792455, Length=205, Percent_Identity=33.6585365853659, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45446752, Length=206, Percent_Identity=27.6699029126214, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4502179, Length=245, Percent_Identity=31.0204081632653, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI6912506, Length=244, Percent_Identity=29.0983606557377, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI4502183, Length=245, Percent_Identity=31.0204081632653, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790362, Length=268, Percent_Identity=81.3432835820896, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1787101, Length=213, Percent_Identity=30.5164319248826, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994009, Length=260, Percent_Identity=35.7692307692308, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71992966, Length=258, Percent_Identity=34.4961240310078, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32564052, Length=259, Percent_Identity=33.2046332046332, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17533613, Length=259, Percent_Identity=33.2046332046332, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17531429, Length=254, Percent_Identity=33.0708661417323, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17531431, Length=254, Percent_Identity=33.0708661417323, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17544068, Length=256, Percent_Identity=35.9375, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71992961, Length=258, Percent_Identity=31.0077519379845, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17558372, Length=258, Percent_Identity=24.8062015503876, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71993722, Length=260, Percent_Identity=26.9230769230769, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=213, Percent_Identity=41.3145539906103, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322985, Length=288, Percent_Identity=27.0833333333333, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550503, Length=256, Percent_Identity=27.734375, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24762346, Length=248, Percent_Identity=30.241935483871, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24762348, Length=248, Percent_Identity=30.241935483871, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130305, Length=248, Percent_Identity=30.241935483871, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24762344, Length=248, Percent_Identity=30.241935483871, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652747, Length=252, Percent_Identity=27.3809523809524, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45551084, Length=252, Percent_Identity=27.3809523809524, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24762342, Length=245, Percent_Identity=26.530612244898, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28030; Mature: 28030

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTSTLTGQCVAEFLGTGLLIFFGAGCVAALRVAGASFGQWEISIIWGLGVAMAIYLTA
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHH
GVSGAHLNPAVTIALWLFACFERRKVLPFIVAQTAGAFCAAALVYGLYRQLFLDLEQSQH
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVRGTAASLNLAGVFSTYPHPHITFIQAFAVETTITAILMAMIMALTDDGNGIPRGPLAP
HHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LLIGLLIAVIGASMGPLTGFALNPARDFGPKLFTSLAGWGSIAFTGGLAIPYFLVPLLAP
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHH
VVGAIIGAFLYRKLIGRHLPCECGIDE
HHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNQTSTLTGQCVAEFLGTGLLIFFGAGCVAALRVAGASFGQWEISIIWGLGVAMAIYLTA
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHH
GVSGAHLNPAVTIALWLFACFERRKVLPFIVAQTAGAFCAAALVYGLYRQLFLDLEQSQH
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVRGTAASLNLAGVFSTYPHPHITFIQAFAVETTITAILMAMIMALTDDGNGIPRGPLAP
HHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LLIGLLIAVIGASMGPLTGFALNPARDFGPKLFTSLAGWGSIAFTGGLAIPYFLVPLLAP
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHH
VVGAIIGAFLYRKLIGRHLPCECGIDE
HHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]