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The map label for this gene is cls [H]

Identifier: 238895249

GI number: 238895249

Start: 3125951

End: 3127411

Strand: Direct

Name: cls [H]

Synonym: KP1_3301

Alternate gene names: 238895249

Gene position: 3125951-3127411 (Clockwise)

Preceding gene: 238895247

Following gene: 238895250

Centisome position: 59.56

GC content: 58.04

Gene sequence:

>1461_bases
ATGACAACCTTTTACACGGTGGTGAACTGGCTGGTCATTCTGGGGTACTGGCTGTTGATCGCCGGTGTAACATTACGAAT
TCTAATGAAACGCCGCGCTGTCCCGTCCGCCATGGCATGGTTGCTGATCATTTATATCCTGCCGCTGGTGGGGATCATCG
CCTATCTCTCCTTTGGCGAGCTGCATCTCGGCAAGCGTCGGGCCGAGCGCGCCAGGGCGATGTGGCCCTCCACGGCCAAA
TGGCTAAACGACCTCAAAGCCTGCAAACATATCTTCGCGGAAGATAACAGCCCGGTGGCCGAATCCCTGTTCAAGCTGTG
CGAACGGCGTCAGGGCATTGGCGGCGTAAAAGGCAACCAGCTACAGCTGCTGACGGAATCTGACGACGTGATGCAGGCCC
TGATCCGCGATATTCAGCTGGCCCGTCACAACATAGAGATGGTCTTTTATATCTGGCAGCCCGGCGGCATGGCCGACAGC
GTCGCTGAATCCCTGATGGCCGCCGCTCGCCGCGGGGTTCACTGTCGGCTAATGCTCGACTCCGCGGGCAGCGTGGCCTT
TTTCCGCAGCCCATGGGCGGCAATGATGCGTAACGCCGGTATTGAAGTCGTCGAAGCTCTTAAGGTTAACCTGATGCGCG
TATTCCTGCGCCGGATGGACCTGCGTCAGCATCGTAAAATGGTGATGATCGATAACTATATCGCCTACACTGGCAGCATG
AATATGGTCGATCCGCGCTACTTTAAACAGGACTCAGGAGTCGGACAGTGGATTGATCTGATGGCGCGAATGGAAGGCCC
GGTGGCGACCTCGATGGGCATCGTCTACTCCTGCGACTGGGAAATAGAGACCGGCAAGCGCATTCTGCCGCCGCCCCCGG
ACGTCAATATCATGCCGTTCGAAGAGGCCAGCGGACATACTATTCACACGATCGCCTCCGGACCGGGCTTTCCGGAAGAT
CTGATTCATCAGGCTCTCCTCACCGCCGCCTATGCGGCGAAAGAGCATCTGATCATGACCACCCCCTACTTCGTACCCAG
CGACGACCTGCTGCATGCGATCTGTACCGCCGCCCAGCGCGGCGTTGACGTGAGCATTATTTTACCGCGCAAGAACGATT
CGCTGCTGGTAGGCTGGGCCAGCCGGGCCTTCTTCACCGAGCTGCTGGCGGCCGGGGTCAAAATCTATCAGTTTGAGGGC
GGACTGTTGCATACCAAGAGCGTGCTGGTCGATGGCGAGCTAAGTCTGGTGGGGACCGTCAACCTGGATATGCGCAGCCT
GTGGCTCAACTTCGAGATTACCCTGGTCATCGATGACGCCGGTTTCGGCAGCGATCTGGCGGCGGTTCAGGATGATTATA
TCTCGCGCTCGCGCCTGCTGGATGCCCGTCTCTGGTTAAAACGTCCGCTGTGGCAGCGGATCGCCGAGCGACTGTTTTAC
TTCTTCAGCCCGTTGCTGTAA

Upstream 100 bases:

>100_bases
TGCCAGCAACTGACTACCGCTTTCGCTGCGTTTTTATTTGCCAGAAAAATACTGATGCGTAAACTCATCACATATCGTCA
TTCAAAAGGATTTTACATCT

Downstream 100 bases:

>100_bases
AACGTGCCCATCAGATACCGAATGAGTAGCAATCATGGATATGGATTTAAACAACCGCCTGACCGAAGACGAAACGCTCG
AGCAGGCTTACGATATCTTC

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTTFYTVVNWLVILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERARAMWPSTAK
WLNDLKACKHIFAEDNSPVAESLFKLCERRQGIGGVKGNQLQLLTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADS
VAESLMAAARRGVHCRLMLDSAGSVAFFRSPWAAMMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMVMIDNYIAYTGSM
NMVDPRYFKQDSGVGQWIDLMARMEGPVATSMGIVYSCDWEIETGKRILPPPPDVNIMPFEEASGHTIHTIASGPGFPED
LIHQALLTAAYAAKEHLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSLLVGWASRAFFTELLAAGVKIYQFEG
GLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLVIDDAGFGSDLAAVQDDYISRSRLLDARLWLKRPLWQRIAERLFY
FFSPLL

Sequences:

>Translated_486_residues
MTTFYTVVNWLVILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERARAMWPSTAK
WLNDLKACKHIFAEDNSPVAESLFKLCERRQGIGGVKGNQLQLLTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADS
VAESLMAAARRGVHCRLMLDSAGSVAFFRSPWAAMMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMVMIDNYIAYTGSM
NMVDPRYFKQDSGVGQWIDLMARMEGPVATSMGIVYSCDWEIETGKRILPPPPDVNIMPFEEASGHTIHTIASGPGFPED
LIHQALLTAAYAAKEHLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSLLVGWASRAFFTELLAAGVKIYQFEG
GLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLVIDDAGFGSDLAAVQDDYISRSRLLDARLWLKRPLWQRIAERLFY
FFSPLL
>Mature_485_residues
TTFYTVVNWLVILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERARAMWPSTAKW
LNDLKACKHIFAEDNSPVAESLFKLCERRQGIGGVKGNQLQLLTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADSV
AESLMAAARRGVHCRLMLDSAGSVAFFRSPWAAMMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMVMIDNYIAYTGSMN
MVDPRYFKQDSGVGQWIDLMARMEGPVATSMGIVYSCDWEIETGKRILPPPPDVNIMPFEEASGHTIHTIASGPGFPEDL
IHQALLTAAYAAKEHLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSLLVGWASRAFFTELLAAGVKIYQFEGG
LLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLVIDDAGFGSDLAAVQDDYISRSRLLDARLWLKRPLWQRIAERLFYF
FSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=486, Percent_Identity=93.6213991769547, Blast_Score=952, Evalue=0.0,
Organism=Escherichia coli, GI1787007, Length=386, Percent_Identity=26.4248704663212, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 54771; Mature: 54640

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFYTVVNWLVILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCH
LHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEDNSPVAESLFKLCERRQGIGGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCE
LQLLTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADSVAESLMAAARRGVHCRLMLD
EEEEECCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEC
SAGSVAFFRSPWAAMMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMVMIDNYIAYTGSM
CCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECEEEEECCC
NMVDPRYFKQDSGVGQWIDLMARMEGPVATSMGIVYSCDWEIETGKRILPPPPDVNIMPF
CCCCCHHHCCCCCCHHHHHHHHHHCCCCEECCCEEEEECEEECCCCCCCCCCCCCEEEEC
EEASGHTIHTIASGPGFPEDLIHQALLTAAYAAKEHLIMTTPYFVPSDDLLHAICTAAQR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHC
GVDVSIILPRKNDSLLVGWASRAFFTELLAAGVKIYQFEGGLLHTKSVLVDGELSLVGTV
CCEEEEEEECCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCEEEECEEEECCCEEEEEEE
NLDMRSLWLNFEITLVIDDAGFGSDLAAVQDDYISRSRLLDARLWLKRPLWQRIAERLFY
CCCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
FFSPLL
HHHCCC
>Mature Secondary Structure 
TTFYTVVNWLVILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCH
LHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEDNSPVAESLFKLCERRQGIGGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCE
LQLLTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADSVAESLMAAARRGVHCRLMLD
EEEEECCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEC
SAGSVAFFRSPWAAMMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMVMIDNYIAYTGSM
CCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECEEEEECCC
NMVDPRYFKQDSGVGQWIDLMARMEGPVATSMGIVYSCDWEIETGKRILPPPPDVNIMPF
CCCCCHHHCCCCCCHHHHHHHHHHCCCCEECCCEEEEECEEECCCCCCCCCCCCCEEEEC
EEASGHTIHTIASGPGFPEDLIHQALLTAAYAAKEHLIMTTPYFVPSDDLLHAICTAAQR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHC
GVDVSIILPRKNDSLLVGWASRAFFTELLAAGVKIYQFEGGLLHTKSVLVDGELSLVGTV
CCEEEEEEECCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCEEEECEEEECCCEEEEEEE
NLDMRSLWLNFEITLVIDDAGFGSDLAAVQDDYISRSRLLDARLWLKRPLWQRIAERLFY
CCCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
FFSPLL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA