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The map label for this gene is lacI [H]

Identifier: 238893422

GI number: 238893422

Start: 1263199

End: 1264269

Strand: Direct

Name: lacI [H]

Synonym: KP1_1309

Alternate gene names: 238893422

Gene position: 1263199-1264269 (Clockwise)

Preceding gene: 238893420

Following gene: 238893423

Centisome position: 24.07

GC content: 58.54

Gene sequence:

>1071_bases
ATGAAGTCTAAAAGCGCTACTCTGGAAGACGTCGCACGCCATGCGGGCGTCTCTTACCAGACCGTGTCCAGGGTACTCAA
CAAATCAGCTAATGTATCCGAAGCGACGCGTCGCAAGGTGGAGAAATCGATAGAGGCCTTACGCTATGTGCCAAACCGGC
TGGCGCAGCAGCTGGTGGGGAAGGAGAGCCAGACGGTCGGGCTGGTGACTATTTCTCTGGCCTGGCATGCGCCTTCCCAG
GTGGCGGCGGCGGTAAAACGCTATGCCAACCTGGAAGGTTACCAGGTTCTGATTTCGATGATCGATGAGAGCGTCAACCA
AAGCATCCAGGATTCCATCAATGAGCTGAAATCTCAGCGGGTGGATAAAGTCATTATCAACGTGCCGCTGGAGACCGAAC
AGGCGCAGAAAATCGCTGCTGACAACGATGATATCGTCTGCCTGTTTCTCGACGTTGATCCCTACAGCTCCGTGTTTAAC
GTTTCGTTTAACCCGGCGGACGGCACCCGGGCCAGCGTCAAATATCTCTATGAGATGGGGCATCGGGAGATCGCCCTGCT
GGCGGGGCCGGACAGCTCAGTCTCGGCCAAACTCCGGTTAAAAAGCTGGCTGGAGACCCTGGATGGTTACGGTCTGAAGC
CGGTGACCGTCCTGCACGGCAACTGGGATGCGCAGAGCGGCTACGCCGGCGCGCTGCAGATGCTGCGCGAAACGCCTAAT
TTCAGCGCTGTGCTGGTGGGTAATGATCAGATGGCGCTAGGGGTGCTCAGCGCCTTCCATCAGCATCAGGTGGCGGTGCC
GGGTGAGAAGTCGGTTATTGGCTATGACGATACCTACGAAAGCTCCTTTTTCTATCCGGCGCTGAGTACCGTGTCGCTCG
ACCTCGACCTGCAGGGGAAAGAGGCGGTGCGGCGGATCCTCGCCAGCACCAGTGGGGCGCCTCATACCTCATCCATTCTG
CCGGCGCGCCTGGTGATACGCCATTCCAGCGGCGCCCGAATCGAGCAGGGAAAAGATCTGCAGGCCATTGCAGAACAGCT
CCGCGCCATCGCCCACCGTCTGGCACCCTGA

Upstream 100 bases:

>100_bases
ATACTTTTGTCGCTATTGTTTTGCAGTGAGAGGTGGCGGTGAGCGTTTTTTCAAATGGCCCCTGTTACAATCATGGGATA
ACAACATAAGGAACCGGACC

Downstream 100 bases:

>100_bases
CGCCAAAGGCGGGGAAACTCGCCTTATCACCTTACCCTTAAATTGAGCAGGGTTGATCTTTGTCACAGCACGGCGTACCC
TTAATGTTATGTTATAACGT

Product: lac repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKSKSATLEDVARHAGVSYQTVSRVLNKSANVSEATRRKVEKSIEALRYVPNRLAQQLVGKESQTVGLVTISLAWHAPSQ
VAAAVKRYANLEGYQVLISMIDESVNQSIQDSINELKSQRVDKVIINVPLETEQAQKIAADNDDIVCLFLDVDPYSSVFN
VSFNPADGTRASVKYLYEMGHREIALLAGPDSSVSAKLRLKSWLETLDGYGLKPVTVLHGNWDAQSGYAGALQMLRETPN
FSAVLVGNDQMALGVLSAFHQHQVAVPGEKSVIGYDDTYESSFFYPALSTVSLDLDLQGKEAVRRILASTSGAPHTSSIL
PARLVIRHSSGARIEQGKDLQAIAEQLRAIAHRLAP

Sequences:

>Translated_356_residues
MKSKSATLEDVARHAGVSYQTVSRVLNKSANVSEATRRKVEKSIEALRYVPNRLAQQLVGKESQTVGLVTISLAWHAPSQ
VAAAVKRYANLEGYQVLISMIDESVNQSIQDSINELKSQRVDKVIINVPLETEQAQKIAADNDDIVCLFLDVDPYSSVFN
VSFNPADGTRASVKYLYEMGHREIALLAGPDSSVSAKLRLKSWLETLDGYGLKPVTVLHGNWDAQSGYAGALQMLRETPN
FSAVLVGNDQMALGVLSAFHQHQVAVPGEKSVIGYDDTYESSFFYPALSTVSLDLDLQGKEAVRRILASTSGAPHTSSIL
PARLVIRHSSGARIEQGKDLQAIAEQLRAIAHRLAP
>Mature_356_residues
MKSKSATLEDVARHAGVSYQTVSRVLNKSANVSEATRRKVEKSIEALRYVPNRLAQQLVGKESQTVGLVTISLAWHAPSQ
VAAAVKRYANLEGYQVLISMIDESVNQSIQDSINELKSQRVDKVIINVPLETEQAQKIAADNDDIVCLFLDVDPYSSVFN
VSFNPADGTRASVKYLYEMGHREIALLAGPDSSVSAKLRLKSWLETLDGYGLKPVTVLHGNWDAQSGYAGALQMLRETPN
FSAVLVGNDQMALGVLSAFHQHQVAVPGEKSVIGYDDTYESSFFYPALSTVSLDLDLQGKEAVRRILASTSGAPHTSSIL
PARLVIRHSSGARIEQGKDLQAIAEQLRAIAHRLAP

Specific function: Repressor of the lactose operon. Binds lactose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786540, Length=353, Percent_Identity=42.7762039660057, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=30.9446254071661, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789068, Length=313, Percent_Identity=32.2683706070287, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=28.0966767371601, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790369, Length=345, Percent_Identity=28.1159420289855, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=29.9093655589124, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789202, Length=328, Percent_Identity=28.9634146341463, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1787580, Length=325, Percent_Identity=26.7692307692308, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994940, Length=324, Percent_Identity=24.3827160493827, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790689, Length=320, Percent_Identity=26.25, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790715, Length=335, Percent_Identity=23.2835820895522, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787906, Length=259, Percent_Identity=29.3436293436293, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789456, Length=310, Percent_Identity=28.0645161290323, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786268, Length=158, Percent_Identity=31.6455696202532, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38798; Mature: 38798

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKSATLEDVARHAGVSYQTVSRVLNKSANVSEATRRKVEKSIEALRYVPNRLAQQLVG
CCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KESQTVGLVTISLAWHAPSQVAAAVKRYANLEGYQVLISMIDESVNQSIQDSINELKSQR
CCCCEEEEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKVIINVPLETEQAQKIAADNDDIVCLFLDVDPYSSVFNVSFNPADGTRASVKYLYEMG
HCEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCC
HREIALLAGPDSSVSAKLRLKSWLETLDGYGLKPVTVLHGNWDAQSGYAGALQMLRETPN
CCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCC
FSAVLVGNDQMALGVLSAFHQHQVAVPGEKSVIGYDDTYESSFFYPALSTVSLDLDLQGK
CEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCEECCCCEEEEEEECCCCH
EAVRRILASTSGAPHTSSILPARLVIRHSSGARIEQGKDLQAIAEQLRAIAHRLAP
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSKSATLEDVARHAGVSYQTVSRVLNKSANVSEATRRKVEKSIEALRYVPNRLAQQLVG
CCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KESQTVGLVTISLAWHAPSQVAAAVKRYANLEGYQVLISMIDESVNQSIQDSINELKSQR
CCCCEEEEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKVIINVPLETEQAQKIAADNDDIVCLFLDVDPYSSVFNVSFNPADGTRASVKYLYEMG
HCEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCC
HREIALLAGPDSSVSAKLRLKSWLETLDGYGLKPVTVLHGNWDAQSGYAGALQMLRETPN
CCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCC
FSAVLVGNDQMALGVLSAFHQHQVAVPGEKSVIGYDDTYESSFFYPALSTVSLDLDLQGK
CEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCEECCCCEEEEEEECCCCH
EAVRRILASTSGAPHTSSILPARLVIRHSSGARIEQGKDLQAIAEQLRAIAHRLAP
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3897196 [H]