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The map label for this gene is phoA [H]

Identifier: 238893309

GI number: 238893309

Start: 1151750

End: 1153204

Strand: Direct

Name: phoA [H]

Synonym: KP1_1186

Alternate gene names: 238893309

Gene position: 1151750-1153204 (Clockwise)

Preceding gene: 238893308

Following gene: 238893310

Centisome position: 21.94

GC content: 58.35

Gene sequence:

>1455_bases
TTGTTTTTTTTAAGTATTTTTTACATGGAGATGACAAAAGTGAAATTAAGCGCTCTGTTTATTGCCCTGATTCCTCTACT
GGGCTCGCCGGTTATTCATGCAGAAACTACTGCTGCGCCGGTTCTGGAAAATCGCGCTGCGCAGGGAGATATCACCACTC
CTGGCGGCGCGCGTCGTTTAACAGGCGATCAAACCGAAGCGCTGCGCGCCTCGTTAATCAATAAGCCAGCTAAAAACGTT
ATTTTGCTGATTGGCGATGGCATGGGTGATTCGGAAATTACCGCTGCGCGAAACTATGCCGAGGGGGCGGGCGGTTTCTT
TAAAGGAATTGATGCTCTGCCGTTAACCGGGCAGTACACGCATTATTCGCTGGATAAAAAAACCGGGAAACCGGACTACG
TGACCGACTCGGCGGCCTCCGCCACCGCCTGGACCACCGGCGTGAAGACTTATAACGGCGCGCTGGGCGTCGATATTCAT
GAGAATGCGCATCAGACCATCCTCGAGCTGGCGAAAGCGGCGGGGCTGGCCACCGGCAACGTTTCCACCGCCGAGCTGCA
GGACGCCACCCCCGCGGCGTTGGTAGCGCATGTGACATCGCGTAAATGCTACGGCCCGACGGTCACCAGCGAAAAATGCC
CCAGCAATGCGCTGGAAAAAGGGGGCAAAGGCTCCATTACCGAACAGCTGCTGAACGCCCGACCGGATGTCACCTTGGGC
GGCGGCGCGAAGACCTTTACCGAAACGGCGACGGCGGGCGAGTGGCAGGGCAAAACCCTGCGCGAGCAGGCGCAAGCGCG
CGGCTACCAGATTGTGACCGACGCGGCTTCTCTTGCCGCCGCGACGGAAGCCAGTCAGGATAAACCGCTGCTGGGACTCT
TTGCCGATGGCAATATGCCGGTACGCTGGGAAGGGCCGAAGGCGTCTTATCACGGTAATATCGATAAGCCGCCGGTGACC
TGTACGCCAAACCCGAAGCGTGACGCCTCGGTGCCGACGCTGGCGCAGATGACGGAGAAAGCGATTGACCTGCTCAGTCG
CAACGAGAAAGGTTTCTTCCTGCAAGTCGAAGGCGCTTCCATCGATAAGCAGGACCATGCGGCGAATCCGTGCGGCCAGA
TCGGCGAAACGGTTGATCTTGACGAAGCGGTGCAGAAGGCGCTGGAATTCGCGCGAAAAGACGGTAATACCCTGGTGATC
GTCACCGCCGACCATGCGCATGCCAGCCAGATCATCCCGGCGGATAGCAAAGCCCCGGGGCTGACCCAGGCTCTGAACAC
GCACGATGGCGCGGTGATGGTGATGAGCTACGGCAACTCTGAGGAAGAGTCGATGGAGCACACCGGCACCCAACTGCGCA
TTGCGGCCTACGGTCCGCATGCGGCTAACGTCGTAGGCCTGACCGATCAGACCGACCTGTTCACGACCATGAAAGCTGCC
CTGAGTCTCAAATAA

Upstream 100 bases:

>100_bases
TCTAAAGAAGCTGTTGCGGCATCCTCGTAGCTAGTCTTTGTGACACGACAAGGATGCCGTCATTTTTCTGTCATCTCCCT
TTTATAAAGTCTCCCTCGTT

Downstream 100 bases:

>100_bases
GTCGCTGCCGGTGGCGAAATTGCGTCACCGGCTGGTTTTTTTTCTTTCTGCGTCCAGACTTACAACGTTCCACTCACAGA
AGGATGGTGCGATGAAAATA

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MFFLSIFYMEMTKVKLSALFIALIPLLGSPVIHAETTAAPVLENRAAQGDITTPGGARRLTGDQTEALRASLINKPAKNV
ILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYSLDKKTGKPDYVTDSAASATAWTTGVKTYNGALGVDIH
ENAHQTILELAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPTVTSEKCPSNALEKGGKGSITEQLLNARPDVTLG
GGAKTFTETATAGEWQGKTLREQAQARGYQIVTDAASLAAATEASQDKPLLGLFADGNMPVRWEGPKASYHGNIDKPPVT
CTPNPKRDASVPTLAQMTEKAIDLLSRNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQKALEFARKDGNTLVI
VTADHAHASQIIPADSKAPGLTQALNTHDGAVMVMSYGNSEEESMEHTGTQLRIAAYGPHAANVVGLTDQTDLFTTMKAA
LSLK

Sequences:

>Translated_484_residues
MFFLSIFYMEMTKVKLSALFIALIPLLGSPVIHAETTAAPVLENRAAQGDITTPGGARRLTGDQTEALRASLINKPAKNV
ILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYSLDKKTGKPDYVTDSAASATAWTTGVKTYNGALGVDIH
ENAHQTILELAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPTVTSEKCPSNALEKGGKGSITEQLLNARPDVTLG
GGAKTFTETATAGEWQGKTLREQAQARGYQIVTDAASLAAATEASQDKPLLGLFADGNMPVRWEGPKASYHGNIDKPPVT
CTPNPKRDASVPTLAQMTEKAIDLLSRNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQKALEFARKDGNTLVI
VTADHAHASQIIPADSKAPGLTQALNTHDGAVMVMSYGNSEEESMEHTGTQLRIAAYGPHAANVVGLTDQTDLFTTMKAA
LSLK
>Mature_484_residues
MFFLSIFYMEMTKVKLSALFIALIPLLGSPVIHAETTAAPVLENRAAQGDITTPGGARRLTGDQTEALRASLINKPAKNV
ILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYSLDKKTGKPDYVTDSAASATAWTTGVKTYNGALGVDIH
ENAHQTILELAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPTVTSEKCPSNALEKGGKGSITEQLLNARPDVTLG
GGAKTFTETATAGEWQGKTLREQAQARGYQIVTDAASLAAATEASQDKPLLGLFADGNMPVRWEGPKASYHGNIDKPPVT
CTPNPKRDASVPTLAQMTEKAIDLLSRNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQKALEFARKDGNTLVI
VTADHAHASQIIPADSKAPGLTQALNTHDGAVMVMSYGNSEEESMEHTGTQLRIAAYGPHAANVVGLTDQTDLFTTMKAA
LSLK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=485, Percent_Identity=29.6907216494845, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI94721246, Length=478, Percent_Identity=29.0794979079498, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI157266292, Length=365, Percent_Identity=30.1369863013699, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI116734717, Length=358, Percent_Identity=30.4469273743017, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI294660770, Length=353, Percent_Identity=29.4617563739377, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI294660772, Length=301, Percent_Identity=29.9003322259136, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI48994877, Length=471, Percent_Identity=86.8365180467091, Blast_Score=843, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320689, Length=355, Percent_Identity=33.2394366197183, Blast_Score=136, Evalue=8e-33,
Organism=Drosophila melanogaster, GI21355981, Length=470, Percent_Identity=30.8510638297872, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19921912, Length=394, Percent_Identity=32.2335025380711, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI21355151, Length=478, Percent_Identity=31.5899581589958, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24585213, Length=385, Percent_Identity=30.1298701298701, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21355149, Length=367, Percent_Identity=31.3351498637602, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859923, Length=418, Percent_Identity=28.7081339712919, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24651554, Length=403, Percent_Identity=28.287841191067, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI85725200, Length=391, Percent_Identity=28.6445012787724, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI85815833, Length=391, Percent_Identity=28.6445012787724, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24657827, Length=368, Percent_Identity=30.1630434782609, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21358067, Length=363, Percent_Identity=27.8236914600551, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24657835, Length=401, Percent_Identity=28.428927680798, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 50947; Mature: 50947

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFLSIFYMEMTKVKLSALFIALIPLLGSPVIHAETTAAPVLENRAAQGDITTPGGARRL
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCCCCCCCCCCC
TGDQTEALRASLINKPAKNVILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYT
CCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCCE
HYSLDKKTGKPDYVTDSAASATAWTTGVKTYNGALGVDIHENAHQTILELAKAAGLATGN
EEECCCCCCCCCCCCCCCCCCCEEECCCEEECCEEEEEECCCHHHHHHHHHHHCCCCCCC
VSTAELQDATPAALVAHVTSRKCYGPTVTSEKCPSNALEKGGKGSITEQLLNARPDVTLG
CCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCCEEEC
GGAKTFTETATAGEWQGKTLREQAQARGYQIVTDAASLAAATEASQDKPLLGLFADGNMP
CCCCHHHCCCCCCCCCCHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCEEEEEECCCCC
VRWEGPKASYHGNIDKPPVTCTPNPKRDASVPTLAQMTEKAIDLLSRNEKGFFLQVEGAS
EEECCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
IDKQDHAANPCGQIGETVDLDEAVQKALEFARKDGNTLVIVTADHAHASQIIPADSKAPG
CCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCC
LTQALNTHDGAVMVMSYGNSEEESMEHTGTQLRIAAYGPHAANVVGLTDQTDLFTTMKAA
HHHHHCCCCCEEEEEECCCCHHHHHHHCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHH
LSLK
HCCC
>Mature Secondary Structure
MFFLSIFYMEMTKVKLSALFIALIPLLGSPVIHAETTAAPVLENRAAQGDITTPGGARRL
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCCCCCCCCCCC
TGDQTEALRASLINKPAKNVILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYT
CCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCCE
HYSLDKKTGKPDYVTDSAASATAWTTGVKTYNGALGVDIHENAHQTILELAKAAGLATGN
EEECCCCCCCCCCCCCCCCCCCEEECCCEEECCEEEEEECCCHHHHHHHHHHHCCCCCCC
VSTAELQDATPAALVAHVTSRKCYGPTVTSEKCPSNALEKGGKGSITEQLLNARPDVTLG
CCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCCEEEC
GGAKTFTETATAGEWQGKTLREQAQARGYQIVTDAASLAAATEASQDKPLLGLFADGNMP
CCCCHHHCCCCCCCCCCHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCEEEEEECCCCC
VRWEGPKASYHGNIDKPPVTCTPNPKRDASVPTLAQMTEKAIDLLSRNEKGFFLQVEGAS
EEECCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
IDKQDHAANPCGQIGETVDLDEAVQKALEFARKDGNTLVIVTADHAHASQIIPADSKAPG
CCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCC
LTQALNTHDGAVMVMSYGNSEEESMEHTGTQLRIAAYGPHAANVVGLTDQTDLFTTMKAA
HHHHHCCCCCEEEEEECCCCHHHHHHHCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHH
LSLK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]