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The map label for this gene is phoE [H]

Identifier: 238893262

GI number: 238893262

Start: 1105013

End: 1106065

Strand: Direct

Name: phoE [H]

Synonym: KP1_1135

Alternate gene names: 238893262

Gene position: 1105013-1106065 (Clockwise)

Preceding gene: 238893254

Following gene: 238893264

Centisome position: 21.05

GC content: 54.51

Gene sequence:

>1053_bases
ATGAAAAAGAGTACTCTGGCATTAATGATGATGGGCTTTGTGGCTTCAACAGCGACGCAGGCAGCGGAAGTTTATAATAA
GAACGCGAACAAGCTGGATGTGTACGGCAAGATCAAAGCCATGCACTATTTCAGCGACTATGACAGCAAGGATGGCGATC
AGACCTACGTGCGTTTCGGTATTAAAGGCGAAACGCAGATTAACGACGACCTGACCGGCTATGGCCGTTGGGAATCTGAA
TTCTCCGGTAACAAAACCGAGAGCGACTCCAGTCAGAAAACCCGTCTGGCGTTCGCCGGCGTGAAGCTGAAGAACTACGG
CTCCTTCGATTACGGTCGTAACCTTGGCGCCCTGTACGACGTGGAAGCCTGGACCGATATGTTCCCGGAATTCGGCGGCG
ATTCCTCTGCCCAGACCGATAACTTTATGACCAAGCGCGCCAGCGGCCTGGCGACCTACCGCAACACCGACTTCTTCGGT
CTGGTGGATGGCCTGGATCTGACCCTGCAGTACCAGGGTAAAAACGAAGGCCGTGAAGCGAAGAAACAGAACGGCGACGG
CGTCGGCACCTCGTTAAGCTATGATTTCGGCGGCAGCGACTTCGCCGTCAGCGCGGCCTACACCAGCTCCGACCGTACCA
ACGATCAGAACCTGCTGGCCCGCGGCCAGGGTTCGAAAGCGGAAGCCTGGGCGACCGGCCTGAAATATGACGCCAACAAT
ATCTACCTGGCGACCATGTACTCTGAAACCCGCAAGATGACCCCGATCAGCGGCGGCTTTGCCAACAAAGCGCAGAACTT
TGAAGCGGTGGCGCAGTATCAGTTCGACTTCGGTCTGCGTCCGTCCCTCGGCTATGTGCTGTCGAAAGGGAAGGATATCG
AAGGGGTGGGGAGTGAAGATCTGGTTAACTACATCGACGTGGGCCTGACCTACTACTTCAACAAAAACATGAACGCCTTC
GTGGATTACAAAATCAACCAGCTGAAAAGCGATAACAAACTCGGCATCAACGATGACGACATCGTCGCGCTGGGTATGAC
CTACCAGTTCTGA

Upstream 100 bases:

>100_bases
CATAAATATTTAATATCCCGGCGTTAAAAAACCTCCAGTTTTTGATGGGCGAGCGCCCGCAATTGAATAGCGCGTATAAA
TAAATCACCAGGACTGAATA

Downstream 100 bases:

>100_bases
TTAACCGCAGAACCACCCGCCGCAGCCCTGGCTGCGGCTTTTTTTTAGCGGCGATTCTTCGCCAAATGCAGGGTGTCGCT
GTTCATGGTGATCTGCGCGT

Product: outer membrane phosphoporin protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKKSTLALMMMGFVASTATQAAEVYNKNANKLDVYGKIKAMHYFSDYDSKDGDQTYVRFGIKGETQINDDLTGYGRWESE
FSGNKTESDSSQKTRLAFAGVKLKNYGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFG
LVDGLDLTLQYQGKNEGREAKKQNGDGVGTSLSYDFGGSDFAVSAAYTSSDRTNDQNLLARGQGSKAEAWATGLKYDANN
IYLATMYSETRKMTPISGGFANKAQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGVGSEDLVNYIDVGLTYYFNKNMNAF
VDYKINQLKSDNKLGINDDDIVALGMTYQF

Sequences:

>Translated_350_residues
MKKSTLALMMMGFVASTATQAAEVYNKNANKLDVYGKIKAMHYFSDYDSKDGDQTYVRFGIKGETQINDDLTGYGRWESE
FSGNKTESDSSQKTRLAFAGVKLKNYGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFG
LVDGLDLTLQYQGKNEGREAKKQNGDGVGTSLSYDFGGSDFAVSAAYTSSDRTNDQNLLARGQGSKAEAWATGLKYDANN
IYLATMYSETRKMTPISGGFANKAQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGVGSEDLVNYIDVGLTYYFNKNMNAF
VDYKINQLKSDNKLGINDDDIVALGMTYQF
>Mature_350_residues
MKKSTLALMMMGFVASTATQAAEVYNKNANKLDVYGKIKAMHYFSDYDSKDGDQTYVRFGIKGETQINDDLTGYGRWESE
FSGNKTESDSSQKTRLAFAGVKLKNYGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFG
LVDGLDLTLQYQGKNEGREAKKQNGDGVGTSLSYDFGGSDFAVSAAYTSSDRTNDQNLLARGQGSKAEAWATGLKYDANN
IYLATMYSETRKMTPISGGFANKAQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGVGSEDLVNYIDVGLTYYFNKNMNAF
VDYKINQLKSDNKLGINDDDIVALGMTYQF

Specific function: Uptake of inorganic phosphate, phosphorylated compounds, and some other negatively charged solutes [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family [H]

Homologues:

Organism=Escherichia coli, GI1786436, Length=351, Percent_Identity=84.3304843304843, Blast_Score=612, Evalue=1e-177,
Organism=Escherichia coli, GI1787641, Length=356, Percent_Identity=60.1123595505618, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1787160, Length=364, Percent_Identity=56.8681318681319, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1788544, Length=371, Percent_Identity=56.8733153638814, Blast_Score=400, Evalue=1e-113,

Paralogues:

None

Copy number: 794 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702 [H]

Pfam domain/function: PF00267 Porin_1 [H]

EC number: NA

Molecular weight: Translated: 38724; Mature: 38724

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00576 GRAM_NEG_PORIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSTLALMMMGFVASTATQAAEVYNKNANKLDVYGKIKAMHYFSDYDSKDGDQTYVRFG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCCEEEEEE
IKGETQINDDLTGYGRWESEFSGNKTESDSSQKTRLAFAGVKLKNYGSFDYGRNLGALYD
CCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEE
VEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGLVDGLDLTLQYQGKNEGREA
CHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEECCCCEEEEECCCEEEEEECCCCCCHHH
KKQNGDGVGTSLSYDFGGSDFAVSAAYTSSDRTNDQNLLARGQGSKAEAWATGLKYDANN
HHCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCHHEECCCCCCCHHHHCCCEECCCC
IYLATMYSETRKMTPISGGFANKAQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGVGSED
EEEEEEECCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCHHHHHCCCCCCCCCCHHH
LVNYIDVGLTYYFNKNMNAFVDYKINQLKSDNKLGINDDDIVALGMTYQF
HHHHHHCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKKSTLALMMMGFVASTATQAAEVYNKNANKLDVYGKIKAMHYFSDYDSKDGDQTYVRFG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCCEEEEEE
IKGETQINDDLTGYGRWESEFSGNKTESDSSQKTRLAFAGVKLKNYGSFDYGRNLGALYD
CCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEE
VEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGLVDGLDLTLQYQGKNEGREA
CHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEECCCCEEEEECCCEEEEEECCCCCCHHH
KKQNGDGVGTSLSYDFGGSDFAVSAAYTSSDRTNDQNLLARGQGSKAEAWATGLKYDANN
HHCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCHHEECCCCCCCHHHHCCCEECCCC
IYLATMYSETRKMTPISGGFANKAQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGVGSED
EEEEEEECCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCHHHHHCCCCCCCCCCHHH
LVNYIDVGLTYYFNKNMNAFVDYKINQLKSDNKLGINDDDIVALGMTYQF
HHHHHHCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3032618 [H]