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The map label for this gene is pulH [H]

Identifier: 238893135

GI number: 238893135

Start: 964846

End: 965361

Strand: Reverse

Name: pulH [H]

Synonym: KP1_0986

Alternate gene names: 238893135

Gene position: 965361-964846 (Counterclockwise)

Preceding gene: 238893136

Following gene: 238893134

Centisome position: 18.39

GC content: 68.6

Gene sequence:

>516_bases
GTGTCTCAGCGCGGTTTCACCCTCCTGGAGATGATGCTGGTCCTGCTGCTGATCGGCGTCAGCGCCAGCATGGTGCTGCT
GGCGTTTCCCTCCGCCCGCACCCAGGAGGCCACACAGATCCTGGCGCGCTTTCAGGCGCAACTGGATTTTGTCCGCGAGC
GCGGGCAGCAAACCGGTCAGCTCTTCGGCATTATCATCCACCCTGAGCGCTGGCAGTTTATGCGCCTGCAGCCTGCCGAC
GATAGCGCCCCGGCGGCGGCTGACGACCGGTGGGGCAACGCGCAGTGGCTGCCGCTGCAGGCCGGACGCGTCGCCACGGC
GGAAACCCTCCCGCGGGCCAGGCTGACGCTGCGCTTTCCCGACGGCCAGGCGTGGACGCCGGGCGAGCAGCCCGACGTGC
TTATTTTTCCCGGCGGTGAAGTCACGCCGTTTCAGCTGCGGATTGACGCCGCGACGGGCATCAACGTCGACGCCCAGGGC
GACAGCCAGCCGCTGGCGGCGCGGGAGCAGCCATGA

Upstream 100 bases:

>100_bases
GCCCGGGTCAGCATGGCGCCATCGACGTCTTCAGCGTCGGCCCGGACGGGATGCCCGACACCAATGACGATATCGGCAAC
TGGACCCTCGGGAAGAAATA

Downstream 100 bases:

>100_bases
AGCGTGAAGCCGGAATGACGCTTATCGAAGTGATGGTCGCACTGGTGATTTTCGCCCTCGCTGGCCTGGCGGTGATGCAG
TCCACCCTGCAGCAGACGCG

Product: pullulanase-specific type II secretion system component H

Products: NA

Alternate protein names: Pullulanase secretion protein pulH [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MSQRGFTLLEMMLVLLLIGVSASMVLLAFPSARTQEATQILARFQAQLDFVRERGQQTGQLFGIIIHPERWQFMRLQPAD
DSAPAAADDRWGNAQWLPLQAGRVATAETLPRARLTLRFPDGQAWTPGEQPDVLIFPGGEVTPFQLRIDAATGINVDAQG
DSQPLAAREQP

Sequences:

>Translated_171_residues
MSQRGFTLLEMMLVLLLIGVSASMVLLAFPSARTQEATQILARFQAQLDFVRERGQQTGQLFGIIIHPERWQFMRLQPAD
DSAPAAADDRWGNAQWLPLQAGRVATAETLPRARLTLRFPDGQAWTPGEQPDVLIFPGGEVTPFQLRIDAATGINVDAQG
DSQPLAAREQP
>Mature_170_residues
SQRGFTLLEMMLVLLLIGVSASMVLLAFPSARTQEATQILARFQAQLDFVRERGQQTGQLFGIIIHPERWQFMRLQPADD
SAPAAADDRWGNAQWLPLQAGRVATAETLPRARLTLRFPDGQAWTPGEQPDVLIFPGGEVTPFQLRIDAATGINVDAQGD
SQPLAAREQP

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2165

COG function: function code NU; Type II secretory pathway, pseudopilin PulG

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP H family [H]

Homologues:

Organism=Escherichia coli, GI1789726, Length=156, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002416
- InterPro:   IPR012902
- InterPro:   IPR001120 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 18849; Mature: 18718

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRGFTLLEMMLVLLLIGVSASMVLLAFPSARTQEATQILARFQAQLDFVRERGQQTGQ
CCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
LFGIIIHPERWQFMRLQPADDSAPAAADDRWGNAQWLPLQAGRVATAETLPRARLTLRFP
EEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEECHHCCCCEEEEEECC
DGQAWTPGEQPDVLIFPGGEVTPFQLRIDAATGINVDAQGDSQPLAAREQP
CCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQRGFTLLEMMLVLLLIGVSASMVLLAFPSARTQEATQILARFQAQLDFVRERGQQTGQ
CCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
LFGIIIHPERWQFMRLQPADDSAPAAADDRWGNAQWLPLQAGRVATAETLPRARLTLRFP
EEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEECHHCCCCEEEEEECC
DGQAWTPGEQPDVLIFPGGEVTPFQLRIDAATGINVDAQGDSQPLAAREQP
CCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2129543 [H]