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The map label for this gene is leuA [H]

Identifier: 238893056

GI number: 238893056

Start: 878987

End: 880603

Strand: Reverse

Name: leuA [H]

Synonym: KP1_0896

Alternate gene names: 238893056

Gene position: 880603-878987 (Counterclockwise)

Preceding gene: 238893080

Following gene: 238893055

Centisome position: 16.78

GC content: 55.41

Gene sequence:

>1617_bases
ATGCTCGTCAACAAGGCGCCAGGACACAGACTAAGGATCCAAACCATGAGCCAGCAAGTCATTATTTTCGATACGACCTT
ACGTGATGGCGAACAAGCGTTACAGGCAAGCCTGAGTGTCAAAGAGAAGCTGCAGATCGCACTGGCCCTTGAGCGAATGG
GGGTCGACGTAATGGAAGTCGGTTTCCCGGTATCCTCGCCGGGCGATTTTGAATCGGTGCAAACCATCGCACGCACCATT
AAAAACAGCCGCGTGTGCGCTCTGGCGCGCTGCGTTGAGAAAGATATCGACGTGGCGGCGGAATCGTTAAAAGTGGCCGA
GGCTTTCCGTATTCATACCTTTATTGCCACCTCGCCGATGCATATCGCCACCAAACTGCGCAGTACGCTGGATGAGGTTA
TTGAGCGCGCGATCTATATGGTGAAGCGGGCACGCAACTACACTGACGACGTTGAGTTCTCCTGTGAAGACGCCGGCCGT
ACGCCGATTGCCGATCTGGCTCGCGTCGTGGAAGCGGCGATCAACGCCGGCGCCACCACCATTAATATCCCGGATACCGT
CGGCTATACCATGCCGTTCGAATATGCCAACATCATCAGCGGCCTGTATGATCGCGTGCCGAATATCGACAAAGCAATTA
TTTCCGTCCACACCCATGATGATCTGGGCATCGCTGTCGGTAACGCCCTCGCCGCGGTCCACGCCGGTGCGCGCCAGGTA
GAAGGTGCGATGAACGGTATCGGCGAACGCGCCGGCAACTGCGCGCTGGAAGAAGTGATCATGGCGATCAAAGTGCGCAA
AGACATCATGAACGTGCACACCAACATTAATCATCATGAAATCTGGCGCACCAGCCAGACCGTCAGCCAGATCTGCAATA
TGCCGATCCCGGCGAACAAGGCCATCGTCGGCACCGGCGCCTTCGCCCACTCCTCCGGCATTCACCAGGACGGCGTGCTG
AAGAACCGCGAAAACTACGAGATCATGACCCCGGAATCTATCGGCCTGAACCAGGTGCAACTGAACCTGACTTCCCGCTC
CGGCCGCGCGGCGGTGAAACACCGGATGGAAGAGATGGGCTACCAGGAGAGCGATTACAACCTGGATCATCTGTACGACG
CATTCCTGAAGCTGGCGGACAAAAAAGGCCAGGTCTTCGATTACGATCTGGAAGCGCTGGCCTTTATCAACAAGCAGCAG
GAAGAGCCAGAGCATTTCCGCCTGGATTACTTCAACGTCCAATCCGGATCCAGCGATATCGCCACCGCCTCGATCAAACT
GGTCTGCGGGGATGAGATCAAAACCGAAGCCGCCAACGGCAACGGTCCGGTCGATGCTATCTACCAGGCGATTAATCGCG
TCACCGACTACAACATTGAATTAGTGAAGTATGGCTTAAGCGCGAAAGGCCACGGCAAAGACGCCCTGGGCCAGGTGGAT
ATCGTCGTTGACTACAATGGCCGCCGCTTCCATGGCGTTGGTCTGGCAACGGATATCGTAGAGTCGTCAGCCAAAGCGAT
GGTTCACGTGCTGAACAACATCTGGCGCGCCGCCGAAGTCGAAAAAGAATTGCAACGCAAAGCTCAGAATAAAGAGAACA
ATAAGGAAACCGTGTAA

Upstream 100 bases:

>100_bases
TAAACGCATGCCATTTGCGCGGTAGGCTGTTGGGCGACGTTCAGCGTTAAGTCATCTTCCAGCAAGACTATAAAACCCGC
GCCTTGGCGCGGGTTTTTTT

Downstream 100 bases:

>100_bases
TGTCGAAGAATTACCATATTGCCGTTTTGCCGGGTGATGGCATTGGCCCGGAAGTGATGAACCAGGCGATGAAAGTACTG
GAAGCCGTGCGCCAACGTTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MLVNKAPGHRLRIQTMSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARTI
KNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGR
TPIADLARVVEAAINAGATTINIPDTVGYTMPFEYANIISGLYDRVPNIDKAIISVHTHDDLGIAVGNALAAVHAGARQV
EGAMNGIGERAGNCALEEVIMAIKVRKDIMNVHTNINHHEIWRTSQTVSQICNMPIPANKAIVGTGAFAHSSGIHQDGVL
KNRENYEIMTPESIGLNQVQLNLTSRSGRAAVKHRMEEMGYQESDYNLDHLYDAFLKLADKKGQVFDYDLEALAFINKQQ
EEPEHFRLDYFNVQSGSSDIATASIKLVCGDEIKTEAANGNGPVDAIYQAINRVTDYNIELVKYGLSAKGHGKDALGQVD
IVVDYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV

Sequences:

>Translated_538_residues
MLVNKAPGHRLRIQTMSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARTI
KNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGR
TPIADLARVVEAAINAGATTINIPDTVGYTMPFEYANIISGLYDRVPNIDKAIISVHTHDDLGIAVGNALAAVHAGARQV
EGAMNGIGERAGNCALEEVIMAIKVRKDIMNVHTNINHHEIWRTSQTVSQICNMPIPANKAIVGTGAFAHSSGIHQDGVL
KNRENYEIMTPESIGLNQVQLNLTSRSGRAAVKHRMEEMGYQESDYNLDHLYDAFLKLADKKGQVFDYDLEALAFINKQQ
EEPEHFRLDYFNVQSGSSDIATASIKLVCGDEIKTEAANGNGPVDAIYQAINRVTDYNIELVKYGLSAKGHGKDALGQVD
IVVDYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV
>Mature_538_residues
MLVNKAPGHRLRIQTMSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARTI
KNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGR
TPIADLARVVEAAINAGATTINIPDTVGYTMPFEYANIISGLYDRVPNIDKAIISVHTHDDLGIAVGNALAAVHAGARQV
EGAMNGIGERAGNCALEEVIMAIKVRKDIMNVHTNINHHEIWRTSQTVSQICNMPIPANKAIVGTGAFAHSSGIHQDGVL
KNRENYEIMTPESIGLNQVQLNLTSRSGRAAVKHRMEEMGYQESDYNLDHLYDAFLKLADKKGQVFDYDLEALAFINKQQ
EEPEHFRLDYFNVQSGSSDIATASIKLVCGDEIKTEAANGNGPVDAIYQAINRVTDYNIELVKYGLSAKGHGKDALGQVD
IVVDYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=523, Percent_Identity=92.925430210325, Blast_Score=1024, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320071, Length=359, Percent_Identity=27.5766016713092, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320019, Length=359, Percent_Identity=27.5766016713092, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=541, Percent_Identity=25.3234750462107, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324682, Length=551, Percent_Identity=24.6823956442831, Blast_Score=126, Evalue=7e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 59231; Mature: 59231

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVNKAPGHRLRIQTMSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEV
CCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GFPVSSPGDFESVQTIARTIKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPM
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
HIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINAGATT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEE
INIPDTVGYTMPFEYANIISGLYDRVPNIDKAIISVHTHDDLGIAVGNALAAVHAGARQV
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
EGAMNGIGERAGNCALEEVIMAIKVRKDIMNVHTNINHHEIWRTSQTVSQICNMPIPANK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
AIVGTGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQVQLNLTSRSGRAAVKHRMEEMG
EEEECCCHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCC
YQESDYNLDHLYDAFLKLADKKGQVFDYDLEALAFINKQQEEPEHFRLDYFNVQSGSSDI
CCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCE
ATASIKLVCGDEIKTEAANGNGPVDAIYQAINRVTDYNIELVKYGLSAKGHGKDALGQVD
EEEEEEEEECCCHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEE
IVVDYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV
EEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLVNKAPGHRLRIQTMSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEV
CCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GFPVSSPGDFESVQTIARTIKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPM
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
HIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINAGATT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEE
INIPDTVGYTMPFEYANIISGLYDRVPNIDKAIISVHTHDDLGIAVGNALAAVHAGARQV
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
EGAMNGIGERAGNCALEEVIMAIKVRKDIMNVHTNINHHEIWRTSQTVSQICNMPIPANK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
AIVGTGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQVQLNLTSRSGRAAVKHRMEEMG
EEEECCCHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCC
YQESDYNLDHLYDAFLKLADKKGQVFDYDLEALAFINKQQEEPEHFRLDYFNVQSGSSDI
CCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCE
ATASIKLVCGDEIKTEAANGNGPVDAIYQAINRVTDYNIELVKYGLSAKGHGKDALGQVD
EEEEEEEEECCCHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEE
IVVDYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV
EEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA