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The map label for this gene is celB [H]

Identifier: 238892954

GI number: 238892954

Start: 761323

End: 762666

Strand: Direct

Name: celB [H]

Synonym: KP1_0780

Alternate gene names: 238892954

Gene position: 761323-762666 (Clockwise)

Preceding gene: 238892952

Following gene: 238892957

Centisome position: 14.51

GC content: 55.88

Gene sequence:

>1344_bases
ATGTCTGCTAACCATGCTGCGTTTAATTTGATATTTCGCTTTGTAGAAAATTACATAAGCCCCATAGCAGGGCGGATCTC
GTCGCAGCGCCACGTCATGGCTATCCGTGACGGATTTATCTCAGCGATGCCGTTTATGATCGTCGGATCGTTCCTGTTAG
TGTTTGCTTATCCACCGTTCTCACCGGATACCACCTGGGGTTTTGCCCGCGCCTGGCTGGATCTGGCGAAAGAGTTTGAG
GGGCGTATTCTGACCCCCTTTGATATGACGATGGGGATCATGTCGATCTACATTTGCGCGGCAATCTCCTATAACCTCGG
TAAGCATTACGAGAAATCCAATCAGCTCGATCCCTTTATGTGCGCAATGCTGTCGATCATGGCCTTTTTGCTGATTGCCG
CGCCGAAGACCAATGGCACGCTGCCGGTGGATAGCCTCGGGGGGACCGGGATCTTTACCGCCATCCTGGTGGCGATCTAC
TGCGTGGAGATGATGCGTTTCTTGAAAGCGCACAACATTGGGATCCGCTTGCCGGATCAGGTTCCGCCGATGATCAAAAA
CTCGTTTGATCTGTTGATCCCGGTGCTGGTGGTCGTGCTGACGCTCTATCCGCTGAGCCTGTTTATCCAGCACCATTTTG
ACATGCTGATCCCGCAGGCCATTATGGCCATTTTCAAGCCGCTGGTTTCGGCAGCCGACTCTCTGCCGGCAATTCTGTTG
GCGGTGCTGATCGGCCACCTGCTGTGGTTTGCCGGGATCCACGGTGCGGCGATCGTGTCCGGGATGCTGCAGATGTTCTG
GCTGACTAACCTCGGTATGAACCAGCAGGCGCTGGCCCAGGGCGCGCCTCTGCCGCATATCTTTATGGAGGCGTTCTGGA
CCTTCTTTATCGTGGTCGGAGGTTCCGGGGCGACCATGGGACTGGTGTTCTGTTATCTGCGCAGCCGTTCAGCGCATTTG
CGCTCCATTGGTCGTTTGAGCGTGGTGCCAAGCCTGTTCAATATCAACGAGCCGGTGATCTTTGGGACGCCTATCGTCAT
GAACCCAGTGTTCTTTATTCCGTTCCTGCTGGCGCCGATGGTGAACGCGGTGCTGGCCTGGGCGGCAATGAAGCTGGATC
TGATTGGCCGCGTCATCTCCGTCGTACCATGGACGGCGCCAGCGCCAATTGGCGGCGCCTGGGCGCTGGGCTGGGATTTC
CGGGCGGCGATTCTGGTCATTGTTCTGGCCTGCGTGTCGGCAATCATCTACTTCCCATTCTTTAAGGTATATGAGAAACA
GCTGCTTGCTCAGGAAGCGGAAGAGGCCGAGCGGGCGGAGCAGGAGAGTCAACAGACCGCATAA

Upstream 100 bases:

>100_bases
TTGCACATCCGCCAGCGGCCTGCTGCGTCAAAAAGCGGTCATCGTAACGGCAGGGTAATCGTTGTTGGGCCAGTATCAAT
AGCCTGTCAGGAGGGCTACT

Downstream 100 bases:

>100_bases
ATAGCAACGGGCGGTAAAGGCCGCCCGTTACTTTTCAGGTAAGTGGTAATTATTTAAGCGTACAGTCGCCGCACTGTTGG
ACATCCGGCAGGCGATAGCG

Product: lactose/cellobiose-specific PTS family enzyme IIC component

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Cel; PTS system cellobiose-specific EIIC component [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSANHAAFNLIFRFVENYISPIAGRISSQRHVMAIRDGFISAMPFMIVGSFLLVFAYPPFSPDTTWGFARAWLDLAKEFE
GRILTPFDMTMGIMSIYICAAISYNLGKHYEKSNQLDPFMCAMLSIMAFLLIAAPKTNGTLPVDSLGGTGIFTAILVAIY
CVEMMRFLKAHNIGIRLPDQVPPMIKNSFDLLIPVLVVVLTLYPLSLFIQHHFDMLIPQAIMAIFKPLVSAADSLPAILL
AVLIGHLLWFAGIHGAAIVSGMLQMFWLTNLGMNQQALAQGAPLPHIFMEAFWTFFIVVGGSGATMGLVFCYLRSRSAHL
RSIGRLSVVPSLFNINEPVIFGTPIVMNPVFFIPFLLAPMVNAVLAWAAMKLDLIGRVISVVPWTAPAPIGGAWALGWDF
RAAILVIVLACVSAIIYFPFFKVYEKQLLAQEAEEAERAEQESQQTA

Sequences:

>Translated_447_residues
MSANHAAFNLIFRFVENYISPIAGRISSQRHVMAIRDGFISAMPFMIVGSFLLVFAYPPFSPDTTWGFARAWLDLAKEFE
GRILTPFDMTMGIMSIYICAAISYNLGKHYEKSNQLDPFMCAMLSIMAFLLIAAPKTNGTLPVDSLGGTGIFTAILVAIY
CVEMMRFLKAHNIGIRLPDQVPPMIKNSFDLLIPVLVVVLTLYPLSLFIQHHFDMLIPQAIMAIFKPLVSAADSLPAILL
AVLIGHLLWFAGIHGAAIVSGMLQMFWLTNLGMNQQALAQGAPLPHIFMEAFWTFFIVVGGSGATMGLVFCYLRSRSAHL
RSIGRLSVVPSLFNINEPVIFGTPIVMNPVFFIPFLLAPMVNAVLAWAAMKLDLIGRVISVVPWTAPAPIGGAWALGWDF
RAAILVIVLACVSAIIYFPFFKVYEKQLLAQEAEEAERAEQESQQTA
>Mature_446_residues
SANHAAFNLIFRFVENYISPIAGRISSQRHVMAIRDGFISAMPFMIVGSFLLVFAYPPFSPDTTWGFARAWLDLAKEFEG
RILTPFDMTMGIMSIYICAAISYNLGKHYEKSNQLDPFMCAMLSIMAFLLIAAPKTNGTLPVDSLGGTGIFTAILVAIYC
VEMMRFLKAHNIGIRLPDQVPPMIKNSFDLLIPVLVVVLTLYPLSLFIQHHFDMLIPQAIMAIFKPLVSAADSLPAILLA
VLIGHLLWFAGIHGAAIVSGMLQMFWLTNLGMNQQALAQGAPLPHIFMEAFWTFFIVVGGSGATMGLVFCYLRSRSAHLR
SIGRLSVVPSLFNINEPVIFGTPIVMNPVFFIPFLLAPMVNAVLAWAAMKLDLIGRVISVVPWTAPAPIGGAWALGWDFR
AAILVIVLACVSAIIYFPFFKVYEKQLLAQEAEEAERAEQESQQTA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=448, Percent_Identity=29.4642857142857, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 49286; Mature: 49155

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SANHAAFNLIFRFVENYISPIAGRISSQRHVMAIRDGFISAMPFMIVGSFLLVFAYPPF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SPDTTWGFARAWLDLAKEFEGRILTPFDMTMGIMSIYICAAISYNLGKHYEKSNQLDPFM
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
CAMLSIMAFLLIAAPKTNGTLPVDSLGGTGIFTAILVAIYCVEMMRFLKAHNIGIRLPDQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VPPMIKNSFDLLIPVLVVVLTLYPLSLFIQHHFDMLIPQAIMAIFKPLVSAADSLPAILL
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLIGHLLWFAGIHGAAIVSGMLQMFWLTNLGMNQQALAQGAPLPHIFMEAFWTFFIVVG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCC
GSGATMGLVFCYLRSRSAHLRSIGRLSVVPSLFNINEPVIFGTPIVMNPVFFIPFLLAPM
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHH
VNAVLAWAAMKLDLIGRVISVVPWTAPAPIGGAWALGWDFRAAILVIVLACVSAIIYFPF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHH
FKVYEKQLLAQEAEEAERAEQESQQTA
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8407820 [H]