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The map label for this gene is topB [H]

Identifier: 238892823

GI number: 238892823

Start: 621318

End: 623009

Strand: Reverse

Name: topB [H]

Synonym: KP1_0634

Alternate gene names: 238892823

Gene position: 623009-621318 (Counterclockwise)

Preceding gene: 238892824

Following gene: 238892820

Centisome position: 11.87

GC content: 61.58

Gene sequence:

>1692_bases
ATGGCCGCGCCGGAGGATTACGATCCGGCGCTGAAACACTGGCGGGCAGAGCATCTGCCGTTTATTCCCCCGGCATGGCG
GCTGGTGATCCGCCCGGAGGTGCGCAAGCAGTTCCGTATCATCGAAAAGCTGCTGAAACAGGCGGATGCAGTGGTGATTG
CCACCGATGCCGACCGCGAAGGCGAAACCATCGCCCGTGAAATTCTGGAGCAATGTCGCTGGCGCGGTCCGGTCCAGCGG
CTGTGGCTGTCGGCACTGGATGACGCCAGTATCCACAAGGCGCTTGCTGCGATGCGATCCGGTGACAGTACGTATCCGCT
GTATCAGGCTGGACTGGCGCGCGCCCGCGCCGACTGGCTGGTCGGCATCAACATGACCCGCGCCTGCACGCTGATGAACC
GGCGGCACAAAGGCGTGCTCTCCGTGGGCCGGGTGCAGACGCCGACCTTACGCCTGGTGGTGGAACGCGACCGGGAGATA
TCCCGCTTCACGCCCCGCCCCTGGTGGCGGGTGGACGCCCGGTTGCATGCTGAGGGCACTACCTTTCTGGCGCAGTGGCA
ACCCGATGGGGTGTACTGTGACGACGAAGGCCGCTGTATCCGTGAATCCGCCGCCCGTGAGGCCACTGCGAGCCTGCAAC
AGGCCGGGACCACGCTGGTACTGGATGTGACCACCCGGCGGGAGAAAACGCCGCCACCGCTGGCCTTCACCCTCAGCGCC
CTGCAGCAGGTCTGCTCAAAACAGTGGGGCATGGGCGCGCAGGAGGTACTGGATATCGCCCAGCGCCTGTATGAAACCCA
TAAGGCCACCACCTATCCGCGCACCGACTGCGGCTGGCTGCCGCTCTCCATGCACACTGAAGCACCGCAGGTTCTGGCGG
CGCTGGTCGCCAGCGACATCTCACTGCAGCCCCTCGCCACGCAACTGAATTTGCAACAACACTCCCGGCTATGGGACGAC
AGCAAAATCACCGCCCACCATGGCATCATCCCGACCCGTAAAACGGTAGATCTGACGCAGATGAATGACAGCGAGCGCAA
GGTTTACGGCCTGGTACGCAGCCATTTTCTGGCGCAGTTTCTGTCGGACCATGAGGTGGATAAAACCGACCTGCGCCTGA
ACTGCGCTGGGGAAACGCTGGCTGCCCGAGGCTCAGTCGTGATTGTCAGCGGCTGGCAACAACTGTTTTTACGCGAGATG
GCAACACATTCACCGGAAGAAACGCAGTCACTTCCCGTCCTGCAACAGGGACAAACCTGCCAGGTGGAACAGGTAGACGT
TCGCGCCCGTCAGACTCAACCGCCGGAGCACTACACCGAAGGGACGCTGATTGCCGCGATGAAAAACGCCGCCCGTTTTG
TCAGCGATGAGCGCTTGAAACAGCGGCTGAAAGAGAACGCCGGTATTGGCACCGAAGCCACCCGCGCCGGGATTATCCAG
ACGCTGCTTAAGCGTGGTTATCTGATTAAGCAACGCCGCTTTCTGTTGGCAACGGATACCGCCAGCGCGCTGATTGACGC
CCTGCCGGAAAAGCTGAAAGATCCGGGCACCACTGCACTGTGGGAGCAGATGCTGGATGACATCGCGACGGGGAAAATGG
GGCTGGAGGCGTTCCTGGTGCAGCAGCAAGAAACCGTTACAGCGCTCACGGCTCAAATAGTCAGAGGCCAGAAAAGTGGC
TCATCAGGTTAG

Upstream 100 bases:

>100_bases
GCGCGATATCGCCCGTGTTCTTGACGCCCGTAACCGCAGCGACGGCTACCTGCATAACGCCACCGTGACCATCACCTAGT
GCTTCGGCCATCTGCTGAGC

Downstream 100 bases:

>100_bases
CAAAACGCCCGGCGTTTGCCGGGCGTGAAAACAACGTCAGTCAACGATTACGGCTTGTGTTCAATCGCCGGGTTGTTGTT
GATGGCGATGCGCTGCGGTT

Product: putative DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MAAPEDYDPALKHWRAEHLPFIPPAWRLVIRPEVRKQFRIIEKLLKQADAVVIATDADREGETIAREILEQCRWRGPVQR
LWLSALDDASIHKALAAMRSGDSTYPLYQAGLARARADWLVGINMTRACTLMNRRHKGVLSVGRVQTPTLRLVVERDREI
SRFTPRPWWRVDARLHAEGTTFLAQWQPDGVYCDDEGRCIRESAAREATASLQQAGTTLVLDVTTRREKTPPPLAFTLSA
LQQVCSKQWGMGAQEVLDIAQRLYETHKATTYPRTDCGWLPLSMHTEAPQVLAALVASDISLQPLATQLNLQQHSRLWDD
SKITAHHGIIPTRKTVDLTQMNDSERKVYGLVRSHFLAQFLSDHEVDKTDLRLNCAGETLAARGSVVIVSGWQQLFLREM
ATHSPEETQSLPVLQQGQTCQVEQVDVRARQTQPPEHYTEGTLIAAMKNAARFVSDERLKQRLKENAGIGTEATRAGIIQ
TLLKRGYLIKQRRFLLATDTASALIDALPEKLKDPGTTALWEQMLDDIATGKMGLEAFLVQQQETVTALTAQIVRGQKSG
SSG

Sequences:

>Translated_563_residues
MAAPEDYDPALKHWRAEHLPFIPPAWRLVIRPEVRKQFRIIEKLLKQADAVVIATDADREGETIAREILEQCRWRGPVQR
LWLSALDDASIHKALAAMRSGDSTYPLYQAGLARARADWLVGINMTRACTLMNRRHKGVLSVGRVQTPTLRLVVERDREI
SRFTPRPWWRVDARLHAEGTTFLAQWQPDGVYCDDEGRCIRESAAREATASLQQAGTTLVLDVTTRREKTPPPLAFTLSA
LQQVCSKQWGMGAQEVLDIAQRLYETHKATTYPRTDCGWLPLSMHTEAPQVLAALVASDISLQPLATQLNLQQHSRLWDD
SKITAHHGIIPTRKTVDLTQMNDSERKVYGLVRSHFLAQFLSDHEVDKTDLRLNCAGETLAARGSVVIVSGWQQLFLREM
ATHSPEETQSLPVLQQGQTCQVEQVDVRARQTQPPEHYTEGTLIAAMKNAARFVSDERLKQRLKENAGIGTEATRAGIIQ
TLLKRGYLIKQRRFLLATDTASALIDALPEKLKDPGTTALWEQMLDDIATGKMGLEAFLVQQQETVTALTAQIVRGQKSG
SSG
>Mature_562_residues
AAPEDYDPALKHWRAEHLPFIPPAWRLVIRPEVRKQFRIIEKLLKQADAVVIATDADREGETIAREILEQCRWRGPVQRL
WLSALDDASIHKALAAMRSGDSTYPLYQAGLARARADWLVGINMTRACTLMNRRHKGVLSVGRVQTPTLRLVVERDREIS
RFTPRPWWRVDARLHAEGTTFLAQWQPDGVYCDDEGRCIRESAAREATASLQQAGTTLVLDVTTRREKTPPPLAFTLSAL
QQVCSKQWGMGAQEVLDIAQRLYETHKATTYPRTDCGWLPLSMHTEAPQVLAALVASDISLQPLATQLNLQQHSRLWDDS
KITAHHGIIPTRKTVDLTQMNDSERKVYGLVRSHFLAQFLSDHEVDKTDLRLNCAGETLAARGSVVIVSGWQQLFLREMA
THSPEETQSLPVLQQGQTCQVEQVDVRARQTQPPEHYTEGTLIAAMKNAARFVSDERLKQRLKENAGIGTEATRAGIIQT
LLKRGYLIKQRRFLLATDTASALIDALPEKLKDPGTTALWEQMLDDIATGKMGLEAFLVQQQETVTALTAQIVRGQKSGS
SG

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=527, Percent_Identity=25.2371916508539, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4507635, Length=506, Percent_Identity=25.6916996047431, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788061, Length=566, Percent_Identity=39.0459363957597, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1787529, Length=537, Percent_Identity=26.4432029795158, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32563869, Length=555, Percent_Identity=26.3063063063063, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=540, Percent_Identity=24.0740740740741, Blast_Score=108, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=547, Percent_Identity=27.0566727605119, Blast_Score=106, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24585251, Length=561, Percent_Identity=24.777183600713, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24640096, Length=512, Percent_Identity=25.78125, Blast_Score=113, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 63117; Mature: 62986

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPEDYDPALKHWRAEHLPFIPPAWRLVIRPEVRKQFRIIEKLLKQADAVVIATDADRE
CCCCCCCCHHHHHHHHHCCCCCCCHHEEEECHHHHHHHHHHHHHHHHCCEEEEEECCCCC
GETIAREILEQCRWRGPVQRLWLSALDDASIHKALAAMRSGDSTYPLYQAGLARARADWL
CHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEE
VGINMTRACTLMNRRHKGVLSVGRVQTPTLRLVVERDREISRFTPRPWWRVDARLHAEGT
EECCHHHHHHHHHHHCCCCEECCCCCCCCEEEEECCCCHHHHCCCCCCEEECCEEECCCC
TFLAQWQPDGVYCDDEGRCIRESAAREATASLQQAGTTLVLDVTTRREKTPPPLAFTLSA
EEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHH
LQQVCSKQWGMGAQEVLDIAQRLYETHKATTYPRTDCGWLPLSMHTEAPQVLAALVASDI
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCC
SLQPLATQLNLQQHSRLWDDSKITAHHGIIPTRKTVDLTQMNDSERKVYGLVRSHFLAQF
CCCHHHHHHCHHHHHHCCCCCCEEEECCCCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHH
LSDHEVDKTDLRLNCAGETLAARGSVVIVSGWQQLFLREMATHSPEETQSLPVLQQGQTC
HHCCCCCCCCEEEEECCCHHHCCCCEEEECCHHHHHHHHHHCCCCHHHHCCCCCCCCCCC
QVEQVDVRARQTQPPEHYTEGTLIAAMKNAARFVSDERLKQRLKENAGIGTEATRAGIIQ
CCHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
TLLKRGYLIKQRRFLLATDTASALIDALPEKLKDPGTTALWEQMLDDIATGKMGLEAFLV
HHHHCCCEECCCEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHH
QQQETVTALTAQIVRGQKSGSSG
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AAPEDYDPALKHWRAEHLPFIPPAWRLVIRPEVRKQFRIIEKLLKQADAVVIATDADRE
CCCCCCCHHHHHHHHHCCCCCCCHHEEEECHHHHHHHHHHHHHHHHCCEEEEEECCCCC
GETIAREILEQCRWRGPVQRLWLSALDDASIHKALAAMRSGDSTYPLYQAGLARARADWL
CHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEE
VGINMTRACTLMNRRHKGVLSVGRVQTPTLRLVVERDREISRFTPRPWWRVDARLHAEGT
EECCHHHHHHHHHHHCCCCEECCCCCCCCEEEEECCCCHHHHCCCCCCEEECCEEECCCC
TFLAQWQPDGVYCDDEGRCIRESAAREATASLQQAGTTLVLDVTTRREKTPPPLAFTLSA
EEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHH
LQQVCSKQWGMGAQEVLDIAQRLYETHKATTYPRTDCGWLPLSMHTEAPQVLAALVASDI
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCC
SLQPLATQLNLQQHSRLWDDSKITAHHGIIPTRKTVDLTQMNDSERKVYGLVRSHFLAQF
CCCHHHHHHCHHHHHHCCCCCCEEEECCCCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHH
LSDHEVDKTDLRLNCAGETLAARGSVVIVSGWQQLFLREMATHSPEETQSLPVLQQGQTC
HHCCCCCCCCEEEEECCCHHHCCCCEEEECCHHHHHHHHHHCCCCHHHHCCCCCCCCCCC
QVEQVDVRARQTQPPEHYTEGTLIAAMKNAARFVSDERLKQRLKENAGIGTEATRAGIIQ
CCHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
TLLKRGYLIKQRRFLLATDTASALIDALPEKLKDPGTTALWEQMLDDIATGKMGLEAFLV
HHHHCCCEECCCEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHH
QQQETVTALTAQIVRGQKSGSSG
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]