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The map label for this gene is yadF [H]

Identifier: 238892769

GI number: 238892769

Start: 564688

End: 565368

Strand: Reverse

Name: yadF [H]

Synonym: KP1_0572

Alternate gene names: 238892769

Gene position: 565368-564688 (Counterclockwise)

Preceding gene: 238892773

Following gene: 238892768

Centisome position: 10.77

GC content: 67.11

Gene sequence:

>681_bases
GTGACGACGTTAAAACCTTTGCTGGCCCGCAACCGCAGCTGGGCGCTGCAGAAGTGTCAGCATGACCCGGACTATTTCGA
AAAATGGGTCGACGGGCAGCGGCCCCACTCGCTGTGGATCGGCTGCTCCGACAGCCGCGTGCCCGCCGAGGTGCTCACCG
GCAGCCAGCCCGGCGAGCTCTTTGTCCACCGTAATATCGCCAACATGCTCGATCCCGCCGACGACAACGTCATGAGCGTC
CTGCAGTATGCCCTCCACTACCTTGAGGTGGAGCGGGTGGTGCTCTGCGGCCACTACGGCTGCGGCGGCGTGCAGGCGGC
GCTCTCTCTGCCCACCCTGCCGCTGGCGCAGGAGTCCTCGGCGCTGGCTCGCCGCATCGGCCAGCTGCGCCACACCCTGC
ATCACGAAATTGCGCAGGTCGCCGATGGGTGTTGTGTTGCGGCCTCCCCCGGCGCCAGTGCCAACGCCAGCGCCGACGCG
GAGCGTTCTCGCCATGCGCTGGACGCCCTGGTGGAAGCCAACGTTCGCGCCCAGTTCACCCGTCTGCTGGAGAGTGAGCC
GGTGCAGACGGTGCTCGCCAGCGGGCGGCCGCTCAGCCTGCACGGCTGCGTCTACGATCTGGCTTCCGGCCATTTAACCA
CCCTCGTCGAACACCTCTCACCGCAGGAGCACGCCCCATGA

Upstream 100 bases:

>100_bases
TCCTCAGGCGTGAAGGGTTCCGGTAAACACTATTTTTACTAGTCGCTGTACGAGTCTTGATGGACATATTCACTCGATGA
ATTAACTCAGGAGAATACTT

Downstream 100 bases:

>100_bases
ATACGTTTCGTCAGGACTCTCTCGCCGGCATCGTCGTCTTCCTTGTCGCCCTGCCGCTGTGCCTCGGCATCGCCCAGGCC
AGCGGCTTACCGCCTTTCGT

Product: putative carbonic anhdrase

Products: NA

Alternate protein names: Carbonate dehydratase 2 [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MTTLKPLLARNRSWALQKCQHDPDYFEKWVDGQRPHSLWIGCSDSRVPAEVLTGSQPGELFVHRNIANMLDPADDNVMSV
LQYALHYLEVERVVLCGHYGCGGVQAALSLPTLPLAQESSALARRIGQLRHTLHHEIAQVADGCCVAASPGASANASADA
ERSRHALDALVEANVRAQFTRLLESEPVQTVLASGRPLSLHGCVYDLASGHLTTLVEHLSPQEHAP

Sequences:

>Translated_226_residues
MTTLKPLLARNRSWALQKCQHDPDYFEKWVDGQRPHSLWIGCSDSRVPAEVLTGSQPGELFVHRNIANMLDPADDNVMSV
LQYALHYLEVERVVLCGHYGCGGVQAALSLPTLPLAQESSALARRIGQLRHTLHHEIAQVADGCCVAASPGASANASADA
ERSRHALDALVEANVRAQFTRLLESEPVQTVLASGRPLSLHGCVYDLASGHLTTLVEHLSPQEHAP
>Mature_225_residues
TTLKPLLARNRSWALQKCQHDPDYFEKWVDGQRPHSLWIGCSDSRVPAEVLTGSQPGELFVHRNIANMLDPADDNVMSVL
QYALHYLEVERVVLCGHYGCGGVQAALSLPTLPLAQESSALARRIGQLRHTLHHEIAQVADGCCVAASPGASANASADAE
RSRHALDALVEANVRAQFTRLLESEPVQTVLASGRPLSLHGCVYDLASGHLTTLVEHLSPQEHAP

Specific function: Unknown

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786318, Length=215, Percent_Identity=40.9302325581395, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1786534, Length=193, Percent_Identity=30.5699481865285, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324292, Length=215, Percent_Identity=31.1627906976744, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 24560; Mature: 24429

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLKPLLARNRSWALQKCQHDPDYFEKWVDGQRPHSLWIGCSDSRVPAEVLTGSQPGEL
CCCHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCCCCE
FVHRNIANMLDPADDNVMSVLQYALHYLEVERVVLCGHYGCGGVQAALSLPTLPLAQESS
EEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCCCCCCCHH
ALARRIGQLRHTLHHEIAQVADGCCVAASPGASANASADAERSRHALDALVEANVRAQFT
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLLESEPVQTVLASGRPLSLHGCVYDLASGHLTTLVEHLSPQEHAP
HHHHCCHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTLKPLLARNRSWALQKCQHDPDYFEKWVDGQRPHSLWIGCSDSRVPAEVLTGSQPGEL
CCHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCCCCE
FVHRNIANMLDPADDNVMSVLQYALHYLEVERVVLCGHYGCGGVQAALSLPTLPLAQESS
EEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCCCCCCCHH
ALARRIGQLRHTLHHEIAQVADGCCVAASPGASANASADAERSRHALDALVEANVRAQFT
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLLESEPVQTVLASGRPLSLHGCVYDLASGHLTTLVEHLSPQEHAP
HHHHCCHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 10493123 [H]