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The map label for this gene is yjeA [H]

Identifier: 238892642

GI number: 238892642

Start: 428166

End: 429143

Strand: Direct

Name: yjeA [H]

Synonym: KP1_0426

Alternate gene names: 238892642

Gene position: 428166-429143 (Clockwise)

Preceding gene: 238892636

Following gene: 238892643

Centisome position: 8.16

GC content: 59.3

Gene sequence:

>978_bases
ATGAGCGAGACGGCAACCTGGCAGCCGAGCGCACCCATTCCCAATCTGTTGAAGCGCGCGGCGGTGATGGCGGAAATCCG
CCGTTTCTTTACCGATCGCGGCGTGCTGGAGGTGGAGACGCCTTGCATGAGTCAGGCGACGGTAACGGATATTCATTTGT
TCCCGTTCGAAACCCGTTTCGTCGGCCCGGGCCATTCTCAGGGGCTTAACCTGTACCTGATGACCAGTCCGGAATACCAC
ATGAAGCGCCTGCTGGCGGCAGGTTGCGGCCCGGTCTTCCAGCTGTGCCGCAGCTTCCGTAATGAAGAGATGGGGCGTCA
TCACAATCCGGAATTCACCATGCTGGAGTGGTATCGTCCATGCTATGACATGTATCGCCTGATCAATGAGGTGGACGATC
TGCTGCAGCAGGTGCTGGAGTGCCAACCGGCGGAAAGTCTCTCCTATCAGCAGGCGTTTCAGCGCCATCTGGAAATTGAC
CCGCTCTCTGCCGATAAAGCACAGCTGCGTGAAGTCGCAGCGAAGCTGGATCTGAGCAATATCGCCGATACTGAAGAAGA
TCGCGATACCCTGCTGCAGCTGCTGTTCACCATGGGCGTGGAGCCGCACATCGGCAAAGATCGACCAACGTTCATTTATC
ATTTCCCGGCGACTCAGGCCTCTTTAGCGCAGATCAGCCCGGAAGATCATCGGGTCGCTGAGCGCTTTGAGGTCTATTAC
AAAGGTATTGAGCTGGCGAACGGTTTCCACGAGCTGACCGACGCCCATGAACAGCGCCTGCGGTTTGAGCAGGATAACCG
CAAACGCGCCGCGCGCGGCCTTCCGCAGCAGCCCATCGATAACAACCTGCTGGCGGCGCTGGAGGCGGGTCTGCCGGACT
GCTCCGGCGTGGCGCTGGGCGTTGACCGCGTGGTCATGCTGGCGCTGGGCGCTGAAAGCATCGGCGAAGTGATCGCCTTT
ACGGTAGACCGCGCCTGA

Upstream 100 bases:

>100_bases
GATTAATGGCATGTAGTCTGCCGGAGATTGCACAAAATTTGTTTCACTCGCCGGTTGCGAGTAGACTTCGTGCCCTTAAT
TTGCAATCGGAGAAAGTACC

Downstream 100 bases:

>100_bases
TCGCTTTTTCACCTCCCTCCGCCGGCGGGTGGGGGAGGTGAAAGACGACTCTGAACCTGACACCCTCGCCCCGGCTTTAG
CCCGAATACCCACAATTTCC

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSETATWQPSAPIPNLLKRAAVMAEIRRFFTDRGVLEVETPCMSQATVTDIHLFPFETRFVGPGHSQGLNLYLMTSPEYH
MKRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRPCYDMYRLINEVDDLLQQVLECQPAESLSYQQAFQRHLEID
PLSADKAQLREVAAKLDLSNIADTEEDRDTLLQLLFTMGVEPHIGKDRPTFIYHFPATQASLAQISPEDHRVAERFEVYY
KGIELANGFHELTDAHEQRLRFEQDNRKRAARGLPQQPIDNNLLAALEAGLPDCSGVALGVDRVVMLALGAESIGEVIAF
TVDRA

Sequences:

>Translated_325_residues
MSETATWQPSAPIPNLLKRAAVMAEIRRFFTDRGVLEVETPCMSQATVTDIHLFPFETRFVGPGHSQGLNLYLMTSPEYH
MKRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRPCYDMYRLINEVDDLLQQVLECQPAESLSYQQAFQRHLEID
PLSADKAQLREVAAKLDLSNIADTEEDRDTLLQLLFTMGVEPHIGKDRPTFIYHFPATQASLAQISPEDHRVAERFEVYY
KGIELANGFHELTDAHEQRLRFEQDNRKRAARGLPQQPIDNNLLAALEAGLPDCSGVALGVDRVVMLALGAESIGEVIAF
TVDRA
>Mature_324_residues
SETATWQPSAPIPNLLKRAAVMAEIRRFFTDRGVLEVETPCMSQATVTDIHLFPFETRFVGPGHSQGLNLYLMTSPEYHM
KRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRPCYDMYRLINEVDDLLQQVLECQPAESLSYQQAFQRHLEIDP
LSADKAQLREVAAKLDLSNIADTEEDRDTLLQLLFTMGVEPHIGKDRPTFIYHFPATQASLAQISPEDHRVAERFEVYYK
GIELANGFHELTDAHEQRLRFEQDNRKRAARGLPQQPIDNNLLAALEAGLPDCSGVALGVDRVVMLALGAESIGEVIAFT
VDRA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=338, Percent_Identity=27.810650887574, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI5031815, Length=338, Percent_Identity=27.810650887574, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=87.6923076923077, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1790571, Length=323, Percent_Identity=33.4365325077399, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789256, Length=325, Percent_Identity=30.1538461538462, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71994340, Length=334, Percent_Identity=29.940119760479, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17535925, Length=334, Percent_Identity=29.940119760479, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17535927, Length=334, Percent_Identity=29.940119760479, Blast_Score=130, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6320242, Length=338, Percent_Identity=27.5147928994083, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324256, Length=354, Percent_Identity=26.8361581920904, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24640849, Length=335, Percent_Identity=27.1641791044776, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640851, Length=335, Percent_Identity=27.1641791044776, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 36846; Mature: 36715

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETATWQPSAPIPNLLKRAAVMAEIRRFFTDRGVLEVETPCMSQATVTDIHLFPFETRF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCEEE
VGPGHSQGLNLYLMTSPEYHMKRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRP
CCCCCCCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
CYDMYRLINEVDDLLQQVLECQPAESLSYQQAFQRHLEIDPLSADKAQLREVAAKLDLSN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
IADTEEDRDTLLQLLFTMGVEPHIGKDRPTFIYHFPATQASLAQISPEDHRVAERFEVYY
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHH
KGIELANGFHELTDAHEQRLRFEQDNRKRAARGLPQQPIDNNLLAALEAGLPDCSGVALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHHHH
VDRVVMLALGAESIGEVIAFTVDRA
HHHHHHHHHCHHHHHHHHHHEECCC
>Mature Secondary Structure 
SETATWQPSAPIPNLLKRAAVMAEIRRFFTDRGVLEVETPCMSQATVTDIHLFPFETRF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCEEE
VGPGHSQGLNLYLMTSPEYHMKRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRP
CCCCCCCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
CYDMYRLINEVDDLLQQVLECQPAESLSYQQAFQRHLEIDPLSADKAQLREVAAKLDLSN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
IADTEEDRDTLLQLLFTMGVEPHIGKDRPTFIYHFPATQASLAQISPEDHRVAERFEVYY
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHH
KGIELANGFHELTDAHEQRLRFEQDNRKRAARGLPQQPIDNNLLAALEAGLPDCSGVALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHHHH
VDRVVMLALGAESIGEVIAFTVDRA
HHHHHHHHHCHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA