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The map label for this gene is nadE [H]

Identifier: 238028323

GI number: 238028323

Start: 3153185

End: 3154870

Strand: Reverse

Name: nadE [H]

Synonym: bglu_1g27840

Alternate gene names: 238028323

Gene position: 3154870-3153185 (Counterclockwise)

Preceding gene: 238028326

Following gene: 238028322

Centisome position: 80.76

GC content: 69.99

Gene sequence:

>1686_bases
ATGAAAACCCGTATCGCTCTCGCCCAACTCAACGTCACCGTCGGCGATTTCGCCGGTAACGTCGCCCGCATCGTCGAGGC
CGCGCGCGCCGCGCACGACGATGGTGCGCAGCTGCTCGTCGCGCCGGAGCTCGCGTTGTCCGGGTATCCGCCCGAGGATC
TGCTGCTGCGTCCGGCGTTCTACACCGAGGCGGCCGAGGCGCTGGCGCGGCTGGCCGAGTGCCTGAAGCCGTTCGAGGGG
CTCGCGGTGCTGGTCGGCCATCCGCTGCGCACGCCCAGCGCCGATGGTAATGCAAACCGTCCGATCGGGCGCGGCGCGAC
GCCGGTGGACACCTACAACGCGGCGTCGCTGCTCGTCGGCGGCGCGATCGCGGCCACCTATCGCAAGCAGGACCTGCCGA
ACACCGAGGTGTTCGACGAGAAGCGCTACTTCGCCACCGATCCGCAGCCGTGCGTGTTCGAGCTGAACGGCACGCGCTTC
GGCGTGATCATCTGCGAGGACGCCTGGCACGCCTCGGCCGCCCAGCTCGCGAAGGCCGCCGGCGCCGAGGTGCTGCTGGT
GCCGAACGGCTCGCCGTATCACATGGACAAGGACGCGGTACGGGTCGGGATCGTGCGCGCGCGGATCCGCGAGACGGGGC
TGCCGGTGGTCTACGTGAACCTGGTGGGCGGGCAGGACGAGCTCGTGTTCGACGGCGGCTCGTTCGTGCTCGACGGCGCG
GGCGAGCTGGTCGCGCGCATGCCGCAGTTCGAGGCGGGCCACGCGATCGTCGAGTTCGACGGCGCGCGCCCGCTGCCGGG
CGAGATCGCGGCGCCGCTGCCGGTCGAGGCGCAGGTCTACCGCGCGCTGGTGCTCGGCGTGCGCGACTATATCGGCAAGA
ACGGCTTTCCCGGCGCGCTGATCGGCCTGTCGGGCGGCGTCGACTCGGCGCTGGTGCTGGCGGTGGCCTGCGACGCGCTC
GGCCCCGAGCGGGTGCGCGCCGTGATGATGCCGTCGCGCTACACGGCCGACATTTCCACCACCGACGCGGCCGAGATGGC
GCGCCGCGTGGGCGTGCGCTACGACGAGATCGCCATCGCGCCGATGTTCGACGCGTTTTGCGGCGCGCTCGCCGACGAGT
TCGCCGGCCGCGACGAGGACGCCACCGAGGAGAACATCCAGGCGCGGATTCGCGGCACGCTGCTGATGGCGCTGTCTAAC
AAGTTCGGCTCGATCGTGCTGACCACCGGCAACAAGAGCGAGATGGCGGTGGGCTACTGCACGCTGTACGGCGACATGGC
GGGCGGCTTCGCCGTGATCAAGGACATCGCGAAGACGCTCGTCTATCGTCTCTGCCATTACCGCAACGCGGCCGCCGAGT
TCGGCTCGACCGAGGTGATCCCGGCGCGCATCCTCAGCCGCGCGCCGTCGGCCGAGCTGCGCGAGAACCAGACCGACCAG
GACAGCCTGCCGCCCTACGACGTGCTCGACGCGATCATGCGCATGTACATGGAAGAAGACCAGCCGCTCGCGGCGATCGT
CGCGGCCGGCTACGACGAGGCCGACGTGACGCGCGTCACGCGTCTCATCAAGATCAACGAATACAAGCGGCGCCAGGCGC
CGATCGGGATTCGCGTCACGCACCGCGCGTTCGGCCGCGACTGGCGCTACCCGATCACCTCGCGCTTCACCGAGCGTATC
GACTGA

Upstream 100 bases:

>100_bases
GTCAGAGATCCGTTTGGTTCGGCGCGCGCGACGCGGCGCGCACCGGTGATGCGCCCGCGGGCGCTCGTCACGCGAGCCGC
GAGGCCGCGCAGATTCCGTC

Downstream 100 bases:

>100_bases
CGGCGCGCCGCGCGTAGAATCCGTTCCAGTCATCCATTTACCGGAGCCTCATCATGAAACGCATTACCGCCATCATCAAA
CCGTTCAAGCTCGACGAAGT

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MKTRIALAQLNVTVGDFAGNVARIVEAARAAHDDGAQLLVAPELALSGYPPEDLLLRPAFYTEAAEALARLAECLKPFEG
LAVLVGHPLRTPSADGNANRPIGRGATPVDTYNAASLLVGGAIAATYRKQDLPNTEVFDEKRYFATDPQPCVFELNGTRF
GVIICEDAWHASAAQLAKAAGAEVLLVPNGSPYHMDKDAVRVGIVRARIRETGLPVVYVNLVGGQDELVFDGGSFVLDGA
GELVARMPQFEAGHAIVEFDGARPLPGEIAAPLPVEAQVYRALVLGVRDYIGKNGFPGALIGLSGGVDSALVLAVACDAL
GPERVRAVMMPSRYTADISTTDAAEMARRVGVRYDEIAIAPMFDAFCGALADEFAGRDEDATEENIQARIRGTLLMALSN
KFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCHYRNAAAEFGSTEVIPARILSRAPSAELRENQTDQ
DSLPPYDVLDAIMRMYMEEDQPLAAIVAAGYDEADVTRVTRLIKINEYKRRQAPIGIRVTHRAFGRDWRYPITSRFTERI
D

Sequences:

>Translated_561_residues
MKTRIALAQLNVTVGDFAGNVARIVEAARAAHDDGAQLLVAPELALSGYPPEDLLLRPAFYTEAAEALARLAECLKPFEG
LAVLVGHPLRTPSADGNANRPIGRGATPVDTYNAASLLVGGAIAATYRKQDLPNTEVFDEKRYFATDPQPCVFELNGTRF
GVIICEDAWHASAAQLAKAAGAEVLLVPNGSPYHMDKDAVRVGIVRARIRETGLPVVYVNLVGGQDELVFDGGSFVLDGA
GELVARMPQFEAGHAIVEFDGARPLPGEIAAPLPVEAQVYRALVLGVRDYIGKNGFPGALIGLSGGVDSALVLAVACDAL
GPERVRAVMMPSRYTADISTTDAAEMARRVGVRYDEIAIAPMFDAFCGALADEFAGRDEDATEENIQARIRGTLLMALSN
KFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCHYRNAAAEFGSTEVIPARILSRAPSAELRENQTDQ
DSLPPYDVLDAIMRMYMEEDQPLAAIVAAGYDEADVTRVTRLIKINEYKRRQAPIGIRVTHRAFGRDWRYPITSRFTERI
D
>Mature_561_residues
MKTRIALAQLNVTVGDFAGNVARIVEAARAAHDDGAQLLVAPELALSGYPPEDLLLRPAFYTEAAEALARLAECLKPFEG
LAVLVGHPLRTPSADGNANRPIGRGATPVDTYNAASLLVGGAIAATYRKQDLPNTEVFDEKRYFATDPQPCVFELNGTRF
GVIICEDAWHASAAQLAKAAGAEVLLVPNGSPYHMDKDAVRVGIVRARIRETGLPVVYVNLVGGQDELVFDGGSFVLDGA
GELVARMPQFEAGHAIVEFDGARPLPGEIAAPLPVEAQVYRALVLGVRDYIGKNGFPGALIGLSGGVDSALVLAVACDAL
GPERVRAVMMPSRYTADISTTDAAEMARRVGVRYDEIAIAPMFDAFCGALADEFAGRDEDATEENIQARIRGTLLMALSN
KFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCHYRNAAAEFGSTEVIPARILSRAPSAELRENQTDQ
DSLPPYDVLDAIMRMYMEEDQPLAAIVAAGYDEADVTRVTRLIKINEYKRRQAPIGIRVTHRAFGRDWRYPITSRFTERI
D

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60481; Mature: 60481

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRIALAQLNVTVGDFAGNVARIVEAARAAHDDGAQLLVAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEHHHHCCHHHHHHHHHHHCCCCCCEEEEECHHHHCCCCCHHHEECCHH
YTEAAEALARLAECLKPFEGLAVLVGHPLRTPSADGNANRPIGRGATPVDTYNAASLLVG
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
GAIAATYRKQDLPNTEVFDEKRYFATDPQPCVFELNGTRFGVIICEDAWHASAAQLAKAA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHC
GAEVLLVPNGSPYHMDKDAVRVGIVRARIRETGLPVVYVNLVGGQDELVFDGGSFVLDGA
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCEEECCC
GELVARMPQFEAGHAIVEFDGARPLPGEIAAPLPVEAQVYRALVLGVRDYIGKNGFPGAL
HHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
IGLSGGVDSALVLAVACDALGPERVRAVMMPSRYTADISTTDAAEMARRVGVRYDEIAIA
EEECCCCCHHHHHHHHHHCCCHHHHHEEECCCCEECCCCCCCHHHHHHHHCCCCHHEEEH
PMFDAFCGALADEFAGRDEDATEENIQARIRGTLLMALSNKFGSIVLTTGNKSEMAVGYC
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCEEEEEE
TLYGDMAGGFAVIKDIAKTLVYRLCHYRNAAAEFGSTEVIPARILSRAPSAELRENQTDQ
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHCCCCCC
DSLPPYDVLDAIMRMYMEEDQPLAAIVAAGYDEADVTRVTRLIKINEYKRRQAPIGIRVT
CCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
HRAFGRDWRYPITSRFTERID
HHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKTRIALAQLNVTVGDFAGNVARIVEAARAAHDDGAQLLVAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEHHHHCCHHHHHHHHHHHCCCCCCEEEEECHHHHCCCCCHHHEECCHH
YTEAAEALARLAECLKPFEGLAVLVGHPLRTPSADGNANRPIGRGATPVDTYNAASLLVG
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
GAIAATYRKQDLPNTEVFDEKRYFATDPQPCVFELNGTRFGVIICEDAWHASAAQLAKAA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHC
GAEVLLVPNGSPYHMDKDAVRVGIVRARIRETGLPVVYVNLVGGQDELVFDGGSFVLDGA
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCEEECCC
GELVARMPQFEAGHAIVEFDGARPLPGEIAAPLPVEAQVYRALVLGVRDYIGKNGFPGAL
HHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
IGLSGGVDSALVLAVACDALGPERVRAVMMPSRYTADISTTDAAEMARRVGVRYDEIAIA
EEECCCCCHHHHHHHHHHCCCHHHHHEEECCCCEECCCCCCCHHHHHHHHCCCCHHEEEH
PMFDAFCGALADEFAGRDEDATEENIQARIRGTLLMALSNKFGSIVLTTGNKSEMAVGYC
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCEEEEEE
TLYGDMAGGFAVIKDIAKTLVYRLCHYRNAAAEFGSTEVIPARILSRAPSAELRENQTDQ
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHCCCCCC
DSLPPYDVLDAIMRMYMEEDQPLAAIVAAGYDEADVTRVTRLIKINEYKRRQAPIGIRVT
CCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
HRAFGRDWRYPITSRFTERID
HHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]