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The map label for this gene is pnp [H]

Identifier: 238028147

GI number: 238028147

Start: 2960174

End: 2962303

Strand: Reverse

Name: pnp [H]

Synonym: bglu_1g25980

Alternate gene names: 238028147

Gene position: 2962303-2960174 (Counterclockwise)

Preceding gene: 238028148

Following gene: 238028146

Centisome position: 75.83

GC content: 65.45

Gene sequence:

>2130_bases
ATGTCTCTGTTCAACAAGATCGTGAAGGAATTCCAGTGGGGCCAGCACAAGGTGCGCCTCGAAACGGGTGAAATCGCCCG
TCAGGCAAGCGGCGCCGTGATCGTCGACGTCGAGGACACCGTGGTGCTCGCGACCGTGGTCGGCGCGAAGAACGCGAAGC
CGGGCCAGGATTTCTTCCCGCTGACGGTCGACTACATCGAGAAGACCTACTCCGCCGGCAAGATTCCCGGCGGCTTTTTC
CGCCGCGAAGGCCGTCCGTCGGAGCACGAGACGCTGACCTCGCGCCTGATCGATCGTCCGCTGCGCCCGCTGTTCCCGGA
AGGCTTCTACAACGAAGTCCAGGTCGTGATCCACGTGCTGTCGATCAACCCGGAAGTGCCGGCCGACATCCCCGCGCTGA
TCGGCGCGTCGGCCGCGCTTGCCGTCTCGGGCCTGCCGTTCAACGGCCCGGTCGGCGCCGCGCGCGTGGCCTACATCGAC
AACCAGTACGTGCTGAATCCGACGCGTGACCAGATCAAGGCATCGAGCCTCGATCTCGTCGTTGCCGGGACGGAGCGCGC
GGTGCTGATGGTCGAGTCGGAAGCCGACCAACTGCCGGAAGACGTGATGCTCGGCGCGGTCGTGTTCGGCCACGAGCAGA
TGCAGACCGCGATCGACGCGATCCACGAACTGGTCCGCGAAGGCGGCAAGCCGGAATGGGACTGGCAGCCGGCGCCGAAG
GACGAGGCGCTGATCGCCAACGTGACGGCAGCGGCGCAGGCCGACCTGCTGGCCGCCTACCAGATCCGCGACAAGCAGGC
CCGCTCGGCCAAGCTGAAGGAAGTCTACGCATCGACCTCGGCGAAGCTCGAGGCTGAAGCGGCGGCAGCGGGCACCACCG
CCGCCGACAAGGCGACCGTCGGCAACATCCTGTTCGACATCGAGGCGAAGATCGTCCGTTCGCAGATCCTGAACGGCGAG
CCGCGCATCGACGGCCGCGACACGCGCACCGTGCGCCCGATCGAGATCCGTACCGGCGTGCTGCCGCGCACCCACGGCTC
GGCGCTGTTTACGCGCGGCGAGACGCAGGCGCTGGTGGTGGCGACGCTCGGCACCAAGGGCGACGAGCAGATCATCGATG
CGCTCGAAGGCGAATACCGCGACCGCTTCATGCTCCACTACAACATGCCCCCGTTCGCGACCGGCGAAACGGGCCGCGTC
GGCTCGCCGAAGCGCCGCGAAATCGGCCACGGCCGCCTCGCCAAGCGCGCGCTGGTGGCGTGCCTGCCGAGCGCCGACGA
ATTCGGCTACTCGATCCGCGTCGTCTCGGAAATCACCGAATCGAACGGGTCGTCGTCGATGGCGTCGGTGTGCGGCGGCT
GCCTCGCGCTGATGGACGCGGGCGTGCCGATGAAGGCGCACGTCGCCGGCATCGCGATGGGCCTGATCCTCGAGGACAAC
AAGTTCGCGGTGCTGACCGACATCCTCGGCGATGAAGACCACCTCGGCGACATGGACTTCAAGGTCGCGGGCACCGAAGC
CGGTGTCACCGCGCTGCAGATGGACATCAAGATCCAGGGCATCACGAAGGAAATCATGCAGGTCGCGCTCGCGCAGGCCA
AGGAAGGCCGCATGCACATCCTCGGCAAGATGAAGGAATCGGTGTCGGGTGCGAACACGACGCTGTCCGAATTCGCGCCG
CGCATGATCACTGTCAAGATCAACCCGGAAAAGATTCGCGACGTGATCGGCAAGGGCGGTTCGGTGATCCGTGCGCTGAC
CGAGGAAACCGGCACGACCATCGACATCTCCGACGACGGCGTCGTGACCATCGCGAGCACCAGCAGCGAAGGCATGGCCG
AGGCGAAGAAGCGCATCGAGCAGATCACCGCCGAGATCGAAGTCGGCCAGGTCTACGAAGGCGCGGTGCTGAAGCTCCTC
GACTTCGGCGCGATCGTGAACCTGCTGCCGGGCAAGGATGGCCTGCTGCACATCTCGGAAATCGTCAACGAGCGTGTGAA
GGACATCAACGACTACCTGAAGGAAGGCCAGGTCGTGAAGATCAAGGTGATCCAGGTCGACGAGAAGGGCCGCGTGCGGC
TGTCGGCAAAGGCGCTCCTGAACGAGCAGAACGACGCACCGCAACAGTAA

Upstream 100 bases:

>100_bases
AAAAAACACACCTCTTCCCGCCGCGTCAGGTCGCACCACCGCCGCGCCGATCGTTTTTCCGCGCGGCGCATGCCATCCAT
GAACAAAACAAGGAGCGACC

Downstream 100 bases:

>100_bases
GCGCAACGAAAGCGGCCGGCGCCTGAACGGGCCGGCCGTTTTTCATGCAGGATGGACGGGCTTGCGCCGATTCCGGCGCA
AGCCCGTCCCTATCTTGGAG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 709; Mature: 708

Protein sequence:

>709_residues
MSLFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGAKNAKPGQDFFPLTVDYIEKTYSAGKIPGGFF
RREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSINPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYID
NQYVLNPTRDQIKASSLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
DEALIANVTAAAQADLLAAYQIRDKQARSAKLKEVYASTSAKLEAEAAAAGTTAADKATVGNILFDIEAKIVRSQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALVACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEDN
KFAVLTDILGDEDHLGDMDFKVAGTEAGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMKESVSGANTTLSEFAP
RMITVKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIEQITAEIEVGQVYEGAVLKLL
DFGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQVVKIKVIQVDEKGRVRLSAKALLNEQNDAPQQ

Sequences:

>Translated_709_residues
MSLFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGAKNAKPGQDFFPLTVDYIEKTYSAGKIPGGFF
RREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSINPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYID
NQYVLNPTRDQIKASSLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
DEALIANVTAAAQADLLAAYQIRDKQARSAKLKEVYASTSAKLEAEAAAAGTTAADKATVGNILFDIEAKIVRSQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALVACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEDN
KFAVLTDILGDEDHLGDMDFKVAGTEAGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMKESVSGANTTLSEFAP
RMITVKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIEQITAEIEVGQVYEGAVLKLL
DFGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQVVKIKVIQVDEKGRVRLSAKALLNEQNDAPQQ
>Mature_708_residues
SLFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGAKNAKPGQDFFPLTVDYIEKTYSAGKIPGGFFR
REGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSINPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYIDN
QYVLNPTRDQIKASSLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPKD
EALIANVTAAAQADLLAAYQIRDKQARSAKLKEVYASTSAKLEAEAAAAGTTAADKATVGNILFDIEAKIVRSQILNGEP
RIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRVG
SPKRREIGHGRLAKRALVACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEDNK
FAVLTDILGDEDHLGDMDFKVAGTEAGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMKESVSGANTTLSEFAPR
MITVKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIEQITAEIEVGQVYEGAVLKLLD
FGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQVVKIKVIQVDEKGRVRLSAKALLNEQNDAPQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=714, Percent_Identity=37.9551820728291, Blast_Score=441, Evalue=1e-124,
Organism=Escherichia coli, GI145693187, Length=695, Percent_Identity=65.4676258992806, Blast_Score=932, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=724, Percent_Identity=32.0441988950276, Blast_Score=319, Evalue=4e-87,
Organism=Drosophila melanogaster, GI281362905, Length=705, Percent_Identity=36.1702127659575, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24651641, Length=705, Percent_Identity=36.1702127659575, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24651643, Length=705, Percent_Identity=36.1702127659575, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI161079377, Length=656, Percent_Identity=36.1280487804878, Blast_Score=375, Evalue=1e-104,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76380; Mature: 76249

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGAKNAKPGQDFFP
CCHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCCCCCCC
LTVDYIEKTYSAGKIPGGFFRREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
EEHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEE
SINPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYIDNQYVLNPTRDQIKASSLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCCEEECCCHHHHHHCCEEEE
VAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
EECCCEEEEEEECCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DEALIANVTAAAQADLLAAYQIRDKQARSAKLKEVYASTSAKLEAEAAAAGTTAADKATV
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCHHH
GNILFDIEAKIVRSQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
CCEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALVA
EEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEDN
HCCCCHHCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTEAGVTALQMDIKIQGITKEIMQVALAQAKEGRMHI
CEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCHHH
LGKMKESVSGANTTLSEFAPRMITVKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
HHHHHHHCCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTSSEGMAEAKKRIEQITAEIEVGQVYEGAVLKLLDFGAIVNLLPGKDGLLHISE
EEEEECCCCCHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IVNERVKDINDYLKEGQVVKIKVIQVDEKGRVRLSAKALLNEQNDAPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGAKNAKPGQDFFP
CHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCCCCCCC
LTVDYIEKTYSAGKIPGGFFRREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
EEHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEE
SINPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYIDNQYVLNPTRDQIKASSLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCCEEECCCHHHHHHCCEEEE
VAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
EECCCEEEEEEECCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DEALIANVTAAAQADLLAAYQIRDKQARSAKLKEVYASTSAKLEAEAAAAGTTAADKATV
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCHHH
GNILFDIEAKIVRSQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
CCEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALVA
EEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEDN
HCCCCHHCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTEAGVTALQMDIKIQGITKEIMQVALAQAKEGRMHI
CEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCHHH
LGKMKESVSGANTTLSEFAPRMITVKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
HHHHHHHCCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTSSEGMAEAKKRIEQITAEIEVGQVYEGAVLKLLDFGAIVNLLPGKDGLLHISE
EEEEECCCCCHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IVNERVKDINDYLKEGQVVKIKVIQVDEKGRVRLSAKALLNEQNDAPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA