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The map label for this gene is nuoB [H]

Identifier: 238028142

GI number: 238028142

Start: 2956439

End: 2956918

Strand: Reverse

Name: nuoB [H]

Synonym: bglu_1g25930

Alternate gene names: 238028142

Gene position: 2956918-2956439 (Counterclockwise)

Preceding gene: 238028143

Following gene: 238028141

Centisome position: 75.69

GC content: 63.96

Gene sequence:

>480_bases
ATGAGTATCGAAGGGGTCTTGAAGGAAGGGTTTGTCACCACGACGGCTGACAAGCTGATCAACTGGACGCGCACCGGGTC
GCTGTGGCCCGTCACGTTCGGTCTCGCGTGCTGTGCGGTCGAGATGATGCATGCGGGCGCGGCCCGTTACGATCTCGATC
GTTTCGGCGTGGTGTTCCGTCCGAGTCCGCGCCAGTCCGACGTGATGATCGTCGCCGGCACGCTCTGCAACAAGATGGCG
CCGGCCCTGCGGCGCGTCTATGACCAGATGGCCGAGCCGCGCTGGGTGATTTCGATGGGATCGTGCGCGAACGGCGGCGG
CTATTACCACTATTCCTATTCCGTCGTGCGCGGCTGCGACCGTATCGTGCCCGTCGACGTCTACGTGCCAGGGTGTCCGC
CGACTGCCGAGGCGCTCGTCTATGGCGTGATCCAGCTGCAAGCGAAGATCCGCCGGACCAGCACCATCGCCCGCCAATAA

Upstream 100 bases:

>100_bases
TATCTGGCGAAAGGGTGGCCTCGACTGGGAGTAATGGGCGGAACAATCACCGGTTTGCGTGAGGCGGCCACTCTCCGCCG
CCTGTCAGGAGTGGAAAGCA

Downstream 100 bases:

>100_bases
AAGTCCGAGCCCCCACAATATGGCAAGCAAAATCGAGACCCTCAAAGCCAACCTCGAGGCAGCGCTCGGCGAGCGCGTGG
TCAGCCTGACCGAGGCGGTG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSIEGVLKEGFVTTTADKLINWTRTGSLWPVTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMA
PALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTSTIARQ

Sequences:

>Translated_159_residues
MSIEGVLKEGFVTTTADKLINWTRTGSLWPVTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMA
PALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTSTIARQ
>Mature_158_residues
SIEGVLKEGFVTTTADKLINWTRTGSLWPVTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAP
ALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTSTIARQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=78.0821917808219, Blast_Score=254, Evalue=2e-68,
Organism=Escherichia coli, GI1788624, Length=149, Percent_Identity=51.006711409396, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1789074, Length=138, Percent_Identity=36.231884057971, Blast_Score=98, Evalue=2e-22,
Organism=Escherichia coli, GI1788834, Length=109, Percent_Identity=37.6146788990826, Blast_Score=89, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=145, Percent_Identity=71.0344827586207, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=80.7142857142857, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=80.7142857142857, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=80, Blast_Score=251, Evalue=1e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17502; Mature: 17370

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEGVLKEGFVTTTADKLINWTRTGSLWPVTFGLACCAVEMMHAGAARYDLDRFGVVFR
CCCCHHHHCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTSTIARQ
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIEGVLKEGFVTTTADKLINWTRTGSLWPVTFGLACCAVEMMHAGAARYDLDRFGVVFR
CCCHHHHCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTSTIARQ
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA