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The map label for this gene is fumB [H]

Identifier: 238028088

GI number: 238028088

Start: 2899062

End: 2900585

Strand: Direct

Name: fumB [H]

Synonym: bglu_1g25390

Alternate gene names: 238028088

Gene position: 2899062-2900585 (Clockwise)

Preceding gene: 238028087

Following gene: 238028089

Centisome position: 74.21

GC content: 67.26

Gene sequence:

>1524_bases
ATGACCGTCATCAAACAGGAAGACCTGATCCAGAGCGTTGCGGATTCGCTCCAGTACATCAGCTACTACCATCCGCTCGA
CTACATCGAGGCGCTCGGCCGTGCCTACGAGGCCGAACAGAGCCCGGCCGCGAAGGACGCGATCGCGCAGATCCTGACCA
ACAGCCGGATGTGCGCCGAAGGCCGCCGGCCGATCTGCCAGGACACCGGGATCGTGACGATCTTCGTGAAGGTCGGCATG
AACGTGCGCTGGGACGGCGCGACGATGAGCGTGACCGACATGATCAACGAGGGCGTGCGCCGCGGCTATACGCATCCCGA
CAACGTGCTGCGCGCCTCGATCGTCAACCCGCCCGAAGGCGCCCGCAAGAACACCAGGGACAACACGCCGGCCGTGATCC
ATTACGAGATCGTGCCGGGCGACAAGGTGGACGTGCAGGTCGCGGCCAAGGGCGGCGGCTCCGAGAACAAGTCGAAGTTC
GTGATGCTGAACCCCTCGGACTCGATCGTCGACTGGGTGCTCAAGACCGTGCCGACGATGGGCGCGGGCTGGTGCCCGCC
CGGCATGCTCGGGATCGGCATCGGCGGCACCGCCGAGAAGGCGATGCTGATGGCCAAGGAATCGCTGATGGACGCGATCG
ACATCCAGGACGTCATCAAGCGGGGGCCGAAGGACTGGGCCGAGGAACTGCGCGTCGAGCTGTACGAGAAGGTCAACGCG
CTCGGCATCGGCGCGCAGGGCCTGGGCGGCCTGGCCACCGTGCTCGACGTCAAGATCATGGCCGCGCCGACCCACGCGGC
CTCGAAGCCCGTGGCGATGATCCCGAACTGCGCGGCCACCCGCCATGCGCACTTCACGCTCGACGGCTCGGGCGTCGCGC
GCCTCGACCCGCCGCCGCTCGACGCCTGGCCGAAGGTGCAGTGGGAACCGAACACCGAGACCAGCAAGCGCGTCGACCTC
AACACGCTGACGCCGCAGGAAGTCGCGAGCTGGGCGCCGGGCCAGACGCTGCTGCTATCGGGCAAGATGCTCACCGGCCG
CGACGCCGCGCATAAGCGCATCGCCGACATGCTCGCCAAGGGCGAGACGCTGCCGGTCGACTTCACGAACCGCGTGATCT
ACTACGTCGGCCCGGTCGATCCGGTGCGCGACGAGGCGGTCGGCCCCGCCGGCCCGACCACGGCCACCCGCATGGACAAG
TTCACCGAAACCATGCTCGCGCAGACCGGCCTGATCTCGATGGTCGGCAAGGCCGAGCGCGGCCCGGTGGCGATCGAGGC
GATCAAGAAGCACAAGGCGGCCTATCTGATGGCCGTGGGCGGCGCCGCCTACCTGGTGTCGAAGGCGATCCGCAGCGCCA
AGGTGCTGGCCTTCGAGGATCTCGGCATGGAAGCGATCTACGAGTTCGACGTGGTGGACATGCCGGTGACGGTCGCGGTG
GATTCGTCGGGTACCTCGGTGCATCAGACCGGGCCGAAGGAGTGGCAAGCGAAGATCGGCAAGATTCCGGTCGCCACCGC
CTGA

Upstream 100 bases:

>100_bases
CCGCGCGCGTCTCGCGAGCCGGCCTGAAAAAGCCGGCAAGGGCCGGCACCGGACCGGCCCGACGATTTTTCACACGAACC
GTCCCCACTGAGTCACAGCC

Downstream 100 bases:

>100_bases
GCGCAGCACGATCGGCATTGGCATTGCCGATTGAACGAGAATGATTTTCAGGCCGGATTTCAATCCGGCCTGCTTTACTA
TTAATCATTCTCATTAACGC

Product: putative fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTVIKQEDLIQSVADSLQYISYYHPLDYIEALGRAYEAEQSPAAKDAIAQILTNSRMCAEGRRPICQDTGIVTIFVKVGM
NVRWDGATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTRDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMDAIDIQDVIKRGPKDWAEELRVELYEKVNA
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVAMIPNCAATRHAHFTLDGSGVARLDPPPLDAWPKVQWEPNTETSKRVDL
NTLTPQEVASWAPGQTLLLSGKMLTGRDAAHKRIADMLAKGETLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDK
FTETMLAQTGLISMVGKAERGPVAIEAIKKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVVDMPVTVAV
DSSGTSVHQTGPKEWQAKIGKIPVATA

Sequences:

>Translated_507_residues
MTVIKQEDLIQSVADSLQYISYYHPLDYIEALGRAYEAEQSPAAKDAIAQILTNSRMCAEGRRPICQDTGIVTIFVKVGM
NVRWDGATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTRDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMDAIDIQDVIKRGPKDWAEELRVELYEKVNA
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVAMIPNCAATRHAHFTLDGSGVARLDPPPLDAWPKVQWEPNTETSKRVDL
NTLTPQEVASWAPGQTLLLSGKMLTGRDAAHKRIADMLAKGETLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDK
FTETMLAQTGLISMVGKAERGPVAIEAIKKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVVDMPVTVAV
DSSGTSVHQTGPKEWQAKIGKIPVATA
>Mature_506_residues
TVIKQEDLIQSVADSLQYISYYHPLDYIEALGRAYEAEQSPAAKDAIAQILTNSRMCAEGRRPICQDTGIVTIFVKVGMN
VRWDGATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEGARKNTRDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFV
MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMDAIDIQDVIKRGPKDWAEELRVELYEKVNAL
GIGAQGLGGLATVLDVKIMAAPTHAASKPVAMIPNCAATRHAHFTLDGSGVARLDPPPLDAWPKVQWEPNTETSKRVDLN
TLTPQEVASWAPGQTLLLSGKMLTGRDAAHKRIADMLAKGETLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKF
TETMLAQTGLISMVGKAERGPVAIEAIKKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVVDMPVTVAVD
SSGTSVHQTGPKEWQAKIGKIPVATA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=467, Percent_Identity=32.5481798715203, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1790564, Length=469, Percent_Identity=30.7036247334755, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789442, Length=291, Percent_Identity=29.8969072164948, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1789443, Length=160, Percent_Identity=28.75, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54440; Mature: 54309

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIKQEDLIQSVADSLQYISYYHPLDYIEALGRAYEAEQSPAAKDAIAQILTNSRMCAE
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHC
GRRPICQDTGIVTIFVKVGMNVRWDGATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEG
CCCCCCCCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHEEECCCCCCC
ARKNTRDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLMAKESLMDAIDIQDVIKRGPKDWAEELRVELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVAMIPNCAATRHAHFTLDGSGVARLDPPPL
EECCCCCCCHHHHHHEEEEEECCCCCCCCCEEECCCCCCCCCEEEEECCCCEEECCCCCC
DAWPKVQWEPNTETSKRVDLNTLTPQEVASWAPGQTLLLSGKMLTGRDAAHKRIADMLAK
CCCCCCEECCCCCCCCEEECCCCCHHHHHHCCCCCEEEEECCEECCCHHHHHHHHHHHHC
GETLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTETMLAQTGLISMVGKAER
CCCCCEEECCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GPVAIEAIKKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVVDMPVTVAV
CCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHEEEEEHHCCHHHEEEEEEECCCEEEEE
DSSGTSVHQTGPKEWQAKIGKIPVATA
CCCCCCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVIKQEDLIQSVADSLQYISYYHPLDYIEALGRAYEAEQSPAAKDAIAQILTNSRMCAE
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHC
GRRPICQDTGIVTIFVKVGMNVRWDGATMSVTDMINEGVRRGYTHPDNVLRASIVNPPEG
CCCCCCCCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHEEECCCCCCC
ARKNTRDNTPAVIHYEIVPGDKVDVQVAAKGGGSENKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLMAKESLMDAIDIQDVIKRGPKDWAEELRVELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKIMAAPTHAASKPVAMIPNCAATRHAHFTLDGSGVARLDPPPL
EECCCCCCCHHHHHHEEEEEECCCCCCCCCEEECCCCCCCCCEEEEECCCCEEECCCCCC
DAWPKVQWEPNTETSKRVDLNTLTPQEVASWAPGQTLLLSGKMLTGRDAAHKRIADMLAK
CCCCCCEECCCCCCCCEEECCCCCHHHHHHCCCCCEEEEECCEECCCHHHHHHHHHHHHC
GETLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTETMLAQTGLISMVGKAER
CCCCCEEECCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GPVAIEAIKKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVVDMPVTVAV
CCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHEEEEEHHCCHHHEEEEEEECCCEEEEE
DSSGTSVHQTGPKEWQAKIGKIPVATA
CCCCCCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]