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The map label for this gene is smeIM [H]

Identifier: 238027668

GI number: 238027668

Start: 2278804

End: 2279556

Strand: Reverse

Name: smeIM [H]

Synonym: bglu_1g20890

Alternate gene names: 238027668

Gene position: 2279556-2278804 (Counterclockwise)

Preceding gene: 238027669

Following gene: 238027667

Centisome position: 58.35

GC content: 63.35

Gene sequence:

>753_bases
ATGAGCGACTGGCTTGACCGATGCCACTTCGGAGACTGCCGCGAGCTGCTGCCGATGATCCCGGCCGGCGTGGCTGATGC
CGTCATTGCCGATCCGCCCTATGGCGACACTTCCCTGAAGTGGGATCGCGTGGTCGACGGCTGGATCGATGCATGCGCGC
GAACCCTGAAACCAGCCGGATCGATTTGGGTATTCGGCAGTCTCCGGTTCATCGCGCCCACGTTCGAACTGATGGAGCGC
GCCGGGTTCCGGTACTCGCAGGACATCGTGTGGCGCAAGCAGAACGGCACTGGCTTCCACAATGATCGCTTTCGGCGCGT
CCACGAGCATGCGGTGCTGTTCTATCGCGGCGCTTGGTCCGACGTCTACAAGTGCCCGCAGGTCACGCACGACGCGCGCA
AAAAACAGGTGCGCCGCAAGACTCGAGCAGCTCACTGGGGCGACATCGACCGCGGCCATTACACCAGCGTGGACGGCGGC
CCGCGCCTGCGCACGAGCGTGATCGACATGAGGAATGAGCACGGCCATGCGCTTCACCCTACACAGAAACCCGTCGATCT
GATCGAGCTGATGGTGCGCTACAGCGTGCCACCTGGCGGCATCGTGCTCGACCCTTTCCTCGGCAGCGGCAGTACAGCGC
TCGCCGCCATCCGAAACGGCAGACACTGGATCGGCTGCGAGCTCGATCCGGATTGCCGCGATATGCAGGCGCAGCGGCTG
CAGCAAAGCCCGCTGATCTTGGAAACGATATGA

Upstream 100 bases:

>100_bases
GAGCCGGCGCCAGTACGGGCCGGTCTTTTTCCGGCTTCATCGAAAGCCGCGTGCATCGAGCCGCGCGCCCTTCGATGCAA
CTGACCTACTGGATTTGACC

Downstream 100 bases:

>100_bases
GCGCCTATTACAACGAGATTGACCCGTATGCGGCGCAGTGGCTTCGCAACCTGATCGCCGCCGGCCACATCGCGCCTGGC
GACGTCGACGAACGGAGTAT

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSDWLDRCHFGDCRELLPMIPAGVADAVIADPPYGDTSLKWDRVVDGWIDACARTLKPAGSIWVFGSLRFIAPTFELMER
AGFRYSQDIVWRKQNGTGFHNDRFRRVHEHAVLFYRGAWSDVYKCPQVTHDARKKQVRRKTRAAHWGDIDRGHYTSVDGG
PRLRTSVIDMRNEHGHALHPTQKPVDLIELMVRYSVPPGGIVLDPFLGSGSTALAAIRNGRHWIGCELDPDCRDMQAQRL
QQSPLILETI

Sequences:

>Translated_250_residues
MSDWLDRCHFGDCRELLPMIPAGVADAVIADPPYGDTSLKWDRVVDGWIDACARTLKPAGSIWVFGSLRFIAPTFELMER
AGFRYSQDIVWRKQNGTGFHNDRFRRVHEHAVLFYRGAWSDVYKCPQVTHDARKKQVRRKTRAAHWGDIDRGHYTSVDGG
PRLRTSVIDMRNEHGHALHPTQKPVDLIELMVRYSVPPGGIVLDPFLGSGSTALAAIRNGRHWIGCELDPDCRDMQAQRL
QQSPLILETI
>Mature_249_residues
SDWLDRCHFGDCRELLPMIPAGVADAVIADPPYGDTSLKWDRVVDGWIDACARTLKPAGSIWVFGSLRFIAPTFELMERA
GFRYSQDIVWRKQNGTGFHNDRFRRVHEHAVLFYRGAWSDVYKCPQVTHDARKKQVRRKTRAAHWGDIDRGHYTSVDGGP
RLRTSVIDMRNEHGHALHPTQKPVDLIELMVRYSVPPGGIVLDPFLGSGSTALAAIRNGRHWIGCELDPDCRDMQAQRLQ
QSPLILETI

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=251, Percent_Identity=27.8884462151394, Blast_Score=87, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 28413; Mature: 28282

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDWLDRCHFGDCRELLPMIPAGVADAVIADPPYGDTSLKWDRVVDGWIDACARTLKPAG
CCHHHHHCCCCCHHHHHHHCCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
SIWVFGSLRFIAPTFELMERAGFRYSQDIVWRKQNGTGFHNDRFRRVHEHAVLFYRGAWS
CEEEEECHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHEEEEEECCCH
DVYKCPQVTHDARKKQVRRKTRAAHWGDIDRGHYTSVDGGPRLRTSVIDMRNEHGHALHP
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
TQKPVDLIELMVRYSVPPGGIVLDPFLGSGSTALAAIRNGRHWIGCELDPDCRDMQAQRL
CCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
QQSPLILETI
HCCCCEEECC
>Mature Secondary Structure 
SDWLDRCHFGDCRELLPMIPAGVADAVIADPPYGDTSLKWDRVVDGWIDACARTLKPAG
CHHHHHCCCCCHHHHHHHCCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
SIWVFGSLRFIAPTFELMERAGFRYSQDIVWRKQNGTGFHNDRFRRVHEHAVLFYRGAWS
CEEEEECHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHEEEEEECCCH
DVYKCPQVTHDARKKQVRRKTRAAHWGDIDRGHYTSVDGGPRLRTSVIDMRNEHGHALHP
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
TQKPVDLIELMVRYSVPPGGIVLDPFLGSGSTALAAIRNGRHWIGCELDPDCRDMQAQRL
CCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
QQSPLILETI
HCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]