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The map label for this gene is insH10 [H]

Identifier: 238027497

GI number: 238027497

Start: 2090913

End: 2091905

Strand: Reverse

Name: insH10 [H]

Synonym: bglu_1g19100

Alternate gene names: 238027497

Gene position: 2091905-2090913 (Counterclockwise)

Preceding gene: 238027498

Following gene: 238027496

Centisome position: 53.55

GC content: 60.52

Gene sequence:

>993_bases
ATGAAACGGCAAATGAGCTTTGCGGAAGCGGAAAGCGCGGGCAAGAAGCGCATGACCCGGCGTCAGCGTTTCCTGGACGA
GATGGAGAAGCTCGTGCCGTGGTCGCGACTGCTGACGGCAATCGAGCCGTACTACCCGAAAGGAACACTCGGCCGCCCGC
CGATCGGTCTGGAGCGGATGCTTCGAATCTACTTCCTGCAGCAGTGGTACAGCCTGTCGGACGAAGGGCTGGAGGATGCG
CTGTACGACAGCATCGCGATGCGGGCCTTCGCCGGGATTGATCTGGCCGTGGAGAACGTGCCGGATGCGACCGCGCTGCT
GAAGTTCCGGCGCCTGCTTCTCGAACATGACCTGACGCGCAAGCTGTTCGACGAGATTGGCATATCGCTGTGCGAACGCG
GGCTGATGATGAAGGAAGGCACGCTGGTCGACGCGACGATCATCGAAGCGCCGCCGTCGACCAAGAACGCCGAGAAGCGC
CGCGATCCGGACATGCATCAAACGAAGAAAGGCAACGCGTGGCACTTCGGCATGAAGGCCCACATCGGCGTCGATGCCGA
TTCGGGGCTGGTTCACAGCGTGGTTGGCACTTCCGCCAACGTGTCGGATGTTTCGCAAGCGCACGCGCTGTTGCATGATC
ACGAGCAAGAAGCGCTCGGCGACGCGGGCTATATCGGCGTAGACAAGCGCAATGAAATGAAGGGCCGGTCGGTGAAGTGG
CGCGTGGCGGCCAAGCGCGGAAAGATCAAGGCGATGCAGGATGGTGCGCTGAAGGATCTGATGATCGCGCTTGAGCGAAC
CAAGGCGCAAATCCGCGCGCGGGTCGAGCATCCGTTCCATGTTGTCAAAAACCTGTTCGGCCATTGCAAGGTGCGCTGCA
AGGGACTAGCGAAGAACACGGCGCAACTGTTCAGCCTGTTCGCCTTGGCGAACTTGGTGATTGCGCAAAACCGGTTTCGG
TCGGTTCACGGGAGTAGTCCGTCACGTGTATGA

Upstream 100 bases:

>100_bases
TCCTTTTACTTAAGGAAACACTGATTAATCCGGCTCGGTGGTCATCGCTGACGCAGCCGAGGACGTTGTAGAAGATAGTT
CACGGAACGAGCCCACGACG

Downstream 100 bases:

>100_bases
AAAACGCAGGAAATGAGGCGCTTTCCCGGGTCAAACTTCCCCGAATTCGCTGCGCTTCACTTCGGCATCCGGAGTTTGCG
CGTCAGCATCGCCGACAAGC

Product: transposase, IS4 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKRQMSFAEAESAGKKRMTRRQRFLDEMEKLVPWSRLLTAIEPYYPKGTLGRPPIGLERMLRIYFLQQWYSLSDEGLEDA
LYDSIAMRAFAGIDLAVENVPDATALLKFRRLLLEHDLTRKLFDEIGISLCERGLMMKEGTLVDATIIEAPPSTKNAEKR
RDPDMHQTKKGNAWHFGMKAHIGVDADSGLVHSVVGTSANVSDVSQAHALLHDHEQEALGDAGYIGVDKRNEMKGRSVKW
RVAAKRGKIKAMQDGALKDLMIALERTKAQIRARVEHPFHVVKNLFGHCKVRCKGLAKNTAQLFSLFALANLVIAQNRFR
SVHGSSPSRV

Sequences:

>Translated_330_residues
MKRQMSFAEAESAGKKRMTRRQRFLDEMEKLVPWSRLLTAIEPYYPKGTLGRPPIGLERMLRIYFLQQWYSLSDEGLEDA
LYDSIAMRAFAGIDLAVENVPDATALLKFRRLLLEHDLTRKLFDEIGISLCERGLMMKEGTLVDATIIEAPPSTKNAEKR
RDPDMHQTKKGNAWHFGMKAHIGVDADSGLVHSVVGTSANVSDVSQAHALLHDHEQEALGDAGYIGVDKRNEMKGRSVKW
RVAAKRGKIKAMQDGALKDLMIALERTKAQIRARVEHPFHVVKNLFGHCKVRCKGLAKNTAQLFSLFALANLVIAQNRFR
SVHGSSPSRV
>Mature_330_residues
MKRQMSFAEAESAGKKRMTRRQRFLDEMEKLVPWSRLLTAIEPYYPKGTLGRPPIGLERMLRIYFLQQWYSLSDEGLEDA
LYDSIAMRAFAGIDLAVENVPDATALLKFRRLLLEHDLTRKLFDEIGISLCERGLMMKEGTLVDATIIEAPPSTKNAEKR
RDPDMHQTKKGNAWHFGMKAHIGVDADSGLVHSVVGTSANVSDVSQAHALLHDHEQEALGDAGYIGVDKRNEMKGRSVKW
RVAAKRGKIKAMQDGALKDLMIALERTKAQIRARVEHPFHVVKNLFGHCKVRCKGLAKNTAQLFSLFALANLVIAQNRFR
SVHGSSPSRV

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=313, Percent_Identity=53.0351437699681, Blast_Score=334, Evalue=6e-93,
Organism=Escherichia coli, GI1789921, Length=321, Percent_Identity=52.0249221183801, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1789612, Length=321, Percent_Identity=52.0249221183801, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1789355, Length=321, Percent_Identity=52.0249221183801, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1788518, Length=321, Percent_Identity=52.0249221183801, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1788342, Length=321, Percent_Identity=52.0249221183801, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1787592, Length=321, Percent_Identity=52.0249221183801, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1786877, Length=321, Percent_Identity=52.0249221183801, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1786764, Length=321, Percent_Identity=52.0249221183801, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1786453, Length=321, Percent_Identity=52.0249221183801, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1788303, Length=321, Percent_Identity=51.7133956386293, Blast_Score=332, Evalue=3e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 37101; Mature: 37101

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQMSFAEAESAGKKRMTRRQRFLDEMEKLVPWSRLLTAIEPYYPKGTLGRPPIGLERM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
LRIYFLQQWYSLSDEGLEDALYDSIAMRAFAGIDLAVENVPDATALLKFRRLLLEHDLTR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHHH
KLFDEIGISLCERGLMMKEGTLVDATIIEAPPSTKNAEKRRDPDMHQTKKGNAWHFGMKA
HHHHHHHHHHHHCCCEECCCCEEEEEEEECCCCCCCCHHHCCCCHHHCCCCCEEEECCEE
HIGVDADSGLVHSVVGTSANVSDVSQAHALLHDHEQEALGDAGYIGVDKRNEMKGRSVKW
EECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEE
RVAAKRGKIKAMQDGALKDLMIALERTKAQIRARVEHPFHVVKNLFGHCKVRCKGLAKNT
EEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AQLFSLFALANLVIAQNRFRSVHGSSPSRV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKRQMSFAEAESAGKKRMTRRQRFLDEMEKLVPWSRLLTAIEPYYPKGTLGRPPIGLERM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
LRIYFLQQWYSLSDEGLEDALYDSIAMRAFAGIDLAVENVPDATALLKFRRLLLEHDLTR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHHH
KLFDEIGISLCERGLMMKEGTLVDATIIEAPPSTKNAEKRRDPDMHQTKKGNAWHFGMKA
HHHHHHHHHHHHCCCEECCCCEEEEEEEECCCCCCCCHHHCCCCHHHCCCCCEEEECCEE
HIGVDADSGLVHSVVGTSANVSDVSQAHALLHDHEQEALGDAGYIGVDKRNEMKGRSVKW
EECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEE
RVAAKRGKIKAMQDGALKDLMIALERTKAQIRARVEHPFHVVKNLFGHCKVRCKGLAKNT
EEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AQLFSLFALANLVIAQNRFRSVHGSSPSRV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]